Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,890	A→G	D549G (GAC→GGC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,890	0	A	G	100.0%	161.1 / NA	46	D549G (GAC→GGC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/21); total (25/21)

CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTG  >  NC_000913/4182743‑4183036

cagcagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGt                                                                                                                                                 >  2:200255/1‑151 (MQ=255)

    agTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTacac                                                                                                                                             >  2:505564/1‑151 (MQ=255)

    agTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTacac                                                                                                                                             >  1:233589/1‑151 (MQ=255)

         aaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGAc                                                                                                                                        <  1:397047/151‑1 (MQ=255)

          agagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACt                                                                                                                                       <  1:415691/151‑1 (MQ=255)

            agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTc                                                                                                                                      >  2:528065/1‑150 (MQ=255)

              ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACt                                                                                                                                   >  1:94852/1‑151 (MQ=255)

               tcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACt                                                                                                                                   >  2:450758/1‑150 (MQ=255)

                    ggTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTc                                                                                                                             >  2:450179/1‑151 (MQ=255)

                         cagccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTa                                                                                                                        <  2:38059/151‑1 (MQ=255)

                             cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTc                                                                                                                    >  2:551973/1‑151 (MQ=255)

                                 tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCaa                                                                                                                 >  1:206912/1‑150 (MQ=255)

                                     tcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                                                                                                             >  1:257473/1‑150 (MQ=255)

                                      cAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa                                                                                                            <  2:686724/150‑1 (MQ=255)

                                           tATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcccc                                                                                                       <  2:291630/150‑1 (MQ=255)

                                            aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTg                                                                                                     <  1:572574/151‑1 (MQ=255)

                                                 ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt                                                                                                >  1:4215/1‑151 (MQ=255)

                                                     aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg                                                                                             >  1:222004/1‑150 (MQ=255)

                                                        aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa                                                                                           >  1:238946/1‑149 (MQ=255)

                                                           ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                      >  2:284079/1‑151 (MQ=255)

                                                           ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                      <  1:163627/151‑1 (MQ=255)

                                                                gTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc                                                                                   <  2:686319/149‑1 (MQ=255)

                                                                   tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATc                                                                              >  1:472326/1‑151 (MQ=255)

                                                                            gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg                                                                     >  2:654286/1‑151 (MQ=255)

                                                                                 aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                                                >  1:183758/1‑151 (MQ=255)

                                                                                 aaCGACGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGCTCGAGGCGTACACCCGACTCACTACGGGCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCg                                                                 >  1:521937/1‑150 (MQ=255)

                                                                                             ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGa                                                     <  2:275905/150‑1 (MQ=255)

                                                                                             ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAc                                                    <  2:386966/151‑1 (MQ=255)

                                                                                               gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTa                                                  <  1:466315/151‑1 (MQ=255)

                                                                                                  cTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                                                >  2:260496/1‑150 (MQ=255)

                                                                                                    cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACg                                               <  1:284046/149‑1 (MQ=255)

                                                                                                    cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa                                              <  2:423683/150‑1 (MQ=255)

                                                                                                       cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc                                          >  2:215124/1‑151 (MQ=255)

                                                                                                        ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg                                         >  1:760152/1‑151 (MQ=255)

                                                                                                         cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg                                        >  1:407456/1‑151 (MQ=255)

                                                                                                             cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc                                    <  1:408899/151‑1 (MQ=255)

                                                                                                                 cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg                                >  2:207830/1‑151 (MQ=255)

                                                                                                                      ccGTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt                           <  1:767539/151‑1 (MQ=255)

                                                                                                                        gTGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                         <  2:183791/151‑1 (MQ=255)

                                                                                                                         tGAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                         <  2:521370/150‑1 (MQ=255)

                                                                                                                          gAACGTGCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt                       <  1:676548/151‑1 (MQ=255)

                                                                                                                                gCAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt                  <  1:198675/150‑1 (MQ=255)

                                                                                                                                 cAGGCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa                <  2:669657/151‑1 (MQ=255)

                                                                                                                                   ggCTTCGAAGTTCGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa                >  2:480323/1‑149 (MQ=255)

                                                                                                                                        cGAAGTTCGAGGCGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc         <  2:79816/151‑1 (MQ=255)

                                                                                                                                               cGAGGCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGgtg  >  2:262768/1‑151 (MQ=255)

CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTG  >  NC_000913/4182743‑4183036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A