Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,221,741	C→T	R249R (CGG→CGA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,221,741	0	C	T	100.0%	212.4 / NA	56	R249R (CGG→CGA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/26); total (30/26)

AATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGG  >  NC_000913/4221593‑4221883

aaTCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCg                                                                                                                                              <  1:179695/151‑1 (MQ=255)

              aaaTCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCAt                                                                                                                                <  2:6155/151‑1 (MQ=255)

                 tCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGt                                                                                                                             >  1:768935/1‑151 (MQ=255)

                  ccATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGtt                                                                                                                            <  1:368537/151‑1 (MQ=255)

                    aTCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTAt                                                                                                                          >  2:179833/1‑151 (MQ=255)

                       agagAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATcca                                                                                                                       <  1:452023/151‑1 (MQ=255)

                         agaATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATccacc                                                                                                                     >  2:132194/1‑151 (MQ=255)

                         agaATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATccacc                                                                                                                     >  2:216997/1‑151 (MQ=255)

                          gaATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATccacc                                                                                                                     >  2:91777/1‑150 (MQ=255)

                              aGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTa                                                                                                                 >  2:512840/1‑150 (MQ=255)

                                  tttAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATg                                                                                                            >  1:216676/1‑151 (MQ=255)

                                    tAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGc                                                                                                          >  1:273759/1‑151 (MQ=255)

                                     aaTATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCt                                                                                                         >  2:654899/1‑151 (MQ=255)

                                       tatTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGc                                                                                                       >  2:606061/1‑151 (MQ=255)

                                         ttCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGcc                                                                                                      >  1:419964/1‑150 (MQ=255)

                                          tctcTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCa                                                                                                     <  1:189293/150‑1 (MQ=255)

                                          tctcTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCAt                                                                                                    >  2:669157/1‑151 (MQ=255)

                                             ctTTGATTTCGCTCATAGTTCTTGTTTTATCGTTAGTTACCTTAGGATCATAAACCGATACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAATCGGACCGTCAAAGCCTGGTTATCCACCAGTAATATGGCTGcc                                                                                                      >  1:357567/1‑146 (MQ=255)

                                               ttGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTATTGGGTACATCCTTCAGTTGTGATCTTTATTCTTAGTCGGACCGTCAAAGTATGGTTATCCACCAGTAATATGGCTGCCATAaca                                                                                                >  1:698759/1‑150 (MQ=255)

                                                 gCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACt                                                                                              >  1:660367/1‑150 (MQ=255)

                                                 gCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTg                                                                                             <  2:563505/151‑1 (MQ=255)

                                                   tttCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGAt                                                                                           >  2:360184/1‑151 (MQ=255)

                                                      cGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGcc                                                                                        >  1:628870/1‑151 (MQ=255)

                                                       gCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGcc                                                                                        >  2:703194/1‑150 (MQ=255)

                                                       gCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCa                                                                                       <  2:532422/151‑1 (MQ=255)

                                                       gCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCa                                                                                       <  2:306270/151‑1 (MQ=255)

                                                          cATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCTAtt                                                                                     <  1:132161/150‑1 (MQ=255)

                                                          cATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCAt                                                                                      <  1:560194/149‑1 (MQ=255)

                                                               ttCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTc                                                                               >  2:96975/1‑151 (MQ=255)

                                                                 cTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTcc                                                                              >  1:333320/1‑150 (MQ=255)

                                                                  ttATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCAt                                                                            >  1:507772/1‑151 (MQ=255)

                                                                   tATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTcc                                                                              <  1:300148/148‑1 (MQ=255)

                                                                   tATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCa                                                                             <  2:412185/149‑1 (MQ=255)

                                                                         aTCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTc                                                                      >  2:590219/1‑150 (MQ=255)

                                                                         aTCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCa                                                                     <  2:181694/151‑1 (MQ=255)

                                                                             ttagttaCATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAat                                                                 >  2:459380/1‑151 (MQ=255)

                                                                                gttaCATTAGCATCATAAGCCGAAACAACATAATGAGTACATCCTTCAGTTGTGTTCTTTACGCTTAGTCGAACCGTCAAAGCATGGTTAGCCGCCAGAACTATGGCTGCAATAACACTGATGCCATTTTGTTCCAAATTGTCAAAat                                                                 <  1:483786/148‑1 (MQ=255)

                                                                                   aCATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCt                                                            <  1:493291/150‑1 (MQ=255)

                                                                                     aTTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTc                                                         <  1:258856/151‑1 (MQ=255)

                                                                                     aTTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTc                                                         <  1:136362/151‑1 (MQ=255)

                                                                                       tAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCa                                                        <  1:585289/150‑1 (MQ=255)

                                                                                         ggATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATa                                                      >  2:78706/1‑150 (MQ=255)

                                                                                              aTAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGc                                                <  1:606004/151‑1 (MQ=255)

                                                                                                     gAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAAt                                         >  2:6923/1‑151 (MQ=255)

                                                                                                     gAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAAt                                         >  1:12987/1‑151 (MQ=255)

                                                                                                          cACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCg                                    <  2:666467/151‑1 (MQ=255)

                                                                                                             gTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCg                                    >  1:762439/1‑148 (MQ=255)

                                                                                                               aaTGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgcaggc                               <  2:374790/151‑1 (MQ=255)

                                                                                                                aTGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCgcaggca                              <  2:581047/151‑1 (MQ=255)

                                                                                                                   ggTACATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGgcagca                           <  2:447971/151‑1 (MQ=255)

                                                                                                                      aCATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAgcaacg                        >  1:633736/1‑151 (MQ=255)

                                                                                                                       cATCCTTCAGTTGTGTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAgcaacgc                       <  2:541627/151‑1 (MQ=255)

                                                                                                                                  tgtgTTCTTTATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTc             <  2:177063/150‑1 (MQ=255)

                                                                                                                                          ttATTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCt    >  1:657164/1‑151 (MQ=255)

                                                                                                                                            aTTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTgg  >  2:236176/1‑151 (MQ=255)

                                                                                                                                            aTTCTTAGTCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTg   <  1:10039/150‑1 (MQ=255)

AATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTAGGATCATAAACCGAAACCACGTAATGGGTACATCCTTCAGTTGTGTTCTTTATTCTTAGCCGGACCGTCAAAGCATGGTTATCCACCAGTAATATGGCTGCCATAACACTGATGCCATTTGATTCCATCTTGTCAAAATATGCTTTCATAACTAGCCCGAAATCACCGCAGGCAGCAACGCAACGCCCCTCAGGACGGCTGG  >  NC_000913/4221593‑4221883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A