Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I1 R1	14	47.9	1492993	99.0%	1478063	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,019	G→A	intergenic (‑48/‑142)	fadB ← / → pepQ	fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,019	0	G	A	100.0%	174.5 / NA	46	intergenic (‑48/‑142)	fadB/pepQ	fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/27); total (19/27)

AGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAATGGA  >  NC_000913/4030873‑4031165

aGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAg                                                                                                                                                 >  1:627253/1‑150 (MQ=255)

 gtgtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAgtg                                                                                                                                               <  2:178824/151‑1 (MQ=255)

   gtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAgtgtg                                                                                                                                             >  2:468332/1‑151 (MQ=255)

     cGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTa                                                                                                                                           <  2:224677/151‑1 (MQ=255)

        gTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTAttt                                                                                                                                        <  2:112267/151‑1 (MQ=255)

           tattaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTtgt                                                                                                                                     >  2:444729/1‑151 (MQ=255)

           tattaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTtgt                                                                                                                                     >  1:473402/1‑151 (MQ=255)

           tattaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTtg                                                                                                                                      <  2:254611/150‑1 (MQ=255)

            attaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTtgtg                                                                                                                                    <  2:29775/151‑1 (MQ=255)

             ttaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTgtgt                                                                                                                                   >  2:410316/1‑151 (MQ=255)

             ttaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTgtgt                                                                                                                                   >  1:325765/1‑151 (MQ=255)

                   gAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTaaaaa                                                                                                                             <  1:549271/151‑1 (MQ=255)

                   gAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTaaaa                                                                                                                              >  2:182653/1‑150 (MQ=255)

                   gAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTaaa                                                                                                                               <  2:148912/149‑1 (MQ=255)

                      ccTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAatat                                                                                                                          <  2:117128/151‑1 (MQ=255)

                         ggggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGca                                                                                                                       <  1:26231/151‑1 (MQ=255)

                                      ccAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTa                                                                                                           <  2:225834/150‑1 (MQ=255)

                                        aGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATCTTGTGTTAAAAATATGCAAACAAAAGATTAAAg                                                                                                        <  2:362742/151‑1 (MQ=255)

                                           tCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGaa                                                                                                      <  1:50347/150‑1 (MQ=255)

                                           tCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGaa                                                                                                      <  1:272446/150‑1 (MQ=255)

                                              gCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGc                                                                                                  <  1:67263/151‑1 (MQ=255)

                                                 aTGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGa                                                                                               <  2:318636/151‑1 (MQ=255)

                                                     cATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCGTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTCGTGTTAAAAAGATGCAAACAAAAGATAAAAGAAATGGCGATCt                                                                                            <  2:506911/150‑1 (MQ=255)

                                                          tCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGa                                                                                          <  2:503555/147‑1 (MQ=255)

                                                           ccagccagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACg                                                                                     >  1:28353/1‑151 (MQ=255)

                                                                          aCAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCa                                                                       <  1:638487/150‑1 (MQ=255)

                                                                            aGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGc                                                                    <  1:653039/151‑1 (MQ=255)

                                                                                   cGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAt                                                             <  1:67495/151‑1 (MQ=255)

                                                                                     ccTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGa                                                           <  1:519646/151‑1 (MQ=255)

                                                                                          gTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAt                                                      >  2:462014/1‑151 (MQ=255)

                                                                                            aaaGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtg                                                     >  1:130850/1‑150 (MQ=255)

                                                                                                    tCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTa                                                >  2:50250/1‑147 (MQ=255)

                                                                                                     cAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATg                                           <  1:704728/151‑1 (MQ=255)

                                                                                                          tcCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCgg                                        >  2:530724/1‑149 (MQ=255)

                                                                                                                     cAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTc                            >  1:132589/1‑150 (MQ=255)

                                                                                                                     cAAGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCa                           <  2:59060/151‑1 (MQ=255)

                                                                                                                      aaGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCa                           >  2:648591/1‑150 (MQ=255)

                                                                                                                       aGGTGATCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCaaa                         <  1:719578/151‑1 (MQ=255)

                                                                                                                             tCTGGTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAAc                    >  1:440274/1‑150 (MQ=255)

                                                                                                                                 gTCGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAg               <  1:310849/151‑1 (MQ=255)

                                                                                                                                   cGTACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAggg             >  2:344077/1‑151 (MQ=255)

                                                                                                                                     tACCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGt            >  2:379894/1‑150 (MQ=255)

                                                                                                                                      aCCAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTa           >  1:102734/1‑150 (MQ=255)

                                                                                                                                       ccAGATGAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaa          >  2:491589/1‑150 (MQ=255)

                                                                                                                                             gAGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAATg    <  2:714357/150‑1 (MQ=255)

                                                                                                                                              aGTCAAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAATGGa  <  2:197339/151‑1 (MQ=255)

AGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAATGGA  >  NC_000913/4030873‑4031165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A