Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I2 R1 13 61.7 1951856 97.4% 1901107 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,379 C→T G6D (GGC→GAC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,3790CT100.0% 163.4 / NA 42G6D (GGC→GAC) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/23);  total (19/23)

GTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGAT  >  NC_000913/4128234‑4128526
                                                                                                                                                 |                                                                                                                                                   
gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCt                                                                                                                                                 <  2:235503/150‑1 (MQ=255)
   ggTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCAt                                                                                                                                             >  2:504791/1‑151 (MQ=255)
    gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCAtt                                                                                                                                            <  1:404529/151‑1 (MQ=255)
      aGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCa                                                                                                                                          <  2:553367/151‑1 (MQ=255)
              gCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATgag                                                                                                                                  <  2:20022/151‑1 (MQ=255)
                  gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATAc                                                                                                                              <  1:312050/151‑1 (MQ=255)
                     gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACtt                                                                                                                            <  2:759961/150‑1 (MQ=255)
                     gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTa                                                                                                                           <  2:79989/151‑1 (MQ=255)
                       tCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTaa                                                                                                                          <  2:705859/150‑1 (MQ=255)
                       tCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTaa                                                                                                                          <  1:91821/150‑1 (MQ=255)
                         aCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATcc                                                                                                                       >  2:260917/1‑151 (MQ=255)
                          ccTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCct                                                                                                                      <  2:620/151‑1 (MQ=255)
                            tGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCctct                                                                                                                    >  1:321164/1‑151 (MQ=255)
                              acgacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGTGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTc                                                                                                                  <  2:2710/151‑1 (MQ=255)
                                         gtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                                                                                       <  2:341634/151‑1 (MQ=255)
                                           tCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                                                                                                      >  2:866873/1‑150 (MQ=255)
                                               cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                                                 >  2:130261/1‑151 (MQ=255)
                                                  tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                                                 >  1:788113/1‑148 (MQ=255)
                                                    aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                                                                                            <  2:437293/151‑1 (MQ=255)
                                                    aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                                                                                            <  2:195833/151‑1 (MQ=255)
                                                     ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                                                                                            >  2:545710/1‑150 (MQ=255)
                                                             aaCACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATAATTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTg                                                                                   >  1:311523/1‑151 (MQ=255)
                                                                 ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTACACAGCGTATGGGCTTATATACTCGTCGCAACAAACAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgt                                                                               <  1:636448/151‑1 (MQ=255)
                                                                    tAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTggg                                                                            >  2:671413/1‑151 (MQ=255)
                                                                           aaTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAc                                                                     >  1:17234/1‑151 (MQ=255)
                                                                              ggAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGt                                                                  >  2:861291/1‑151 (MQ=255)
                                                                               gAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGt                                                                  >  2:470749/1‑150 (MQ=255)
                                                                                        aTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCAt                                                        >  1:310150/1‑151 (MQ=255)
                                                                                            ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCATCGATCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACAAGACCACACAGTTGATGTGGGTACTGACCGTAAAACCGCATa                                                       <  1:220115/148‑1 (MQ=255)
                                                                                                       tCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACa                                           <  2:926768/149‑1 (MQ=255)
                                                                                                          ctctTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCGTTCAGCCATGAGATACTTACTCCTCGTCGTCAATAAATTGAGACTTGACCAAAGAGTTGATGTGGGTACTGTCCGTAAACCCACATATTTTACCGTACAggaa                                       <  1:456254/150‑3 (MQ=255)
                                                                                                            cttcttGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGt                                      <  1:66579/149‑1 (MQ=255)
                                                                                                                ttGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGt                                >  2:146061/1‑151 (MQ=255)
                                                                                                                 tGCCGTGCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTAcc                                  <  1:967785/148‑1 (MQ=255)
                                                                                                                       gCTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATc                          <  2:942871/150‑1 (MQ=255)
                                                                                                                        cTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgt                        >  2:683215/1‑151 (MQ=255)
                                                                                                                        cTCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgt                        >  1:402365/1‑151 (MQ=255)
                                                                                                                         tCAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgtg                       >  1:21856/1‑151 (MQ=255)
                                                                                                                          cAGCGTATGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgtg                       <  2:823185/150‑1 (MQ=255)
                                                                                                                           aGCGTATGGGCTGATATATTCGTAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgtgt                      >  1:225316/1‑150 (MQ=255)
                                                                                                                                 tGGGCTGATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCAc               >  1:47049/1‑151 (MQ=255)
                                                                                                                                       gATATATTCGTCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCg         <  2:310192/151‑1 (MQ=255)
                                                                                                                                              tcgtcgCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGAt  >  1:86000/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGAT  >  NC_000913/4128234‑4128526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A