Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F50 I1 R1 10 71.6 2231174 98.9% 2206631 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,336,073 C→G G141A (GGT→GCT)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,0730CG100.0% 226.4 / NA 56G141A (GGT→GCT) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (32/24);  total (32/24)

TCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGA  >  NC_000913/3335926‑3336218
                                                                                                                                                   |                                                                                                                                                 
tCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGct                                                                                                                                                 <  1:144004/150‑1 (MQ=255)
   ggTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACAGGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGcttctt                                                                                                                                             <  1:362234/151‑1 (MQ=255)
    gTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCttcttc                                                                                                                                            >  2:328309/1‑151 (MQ=255)
     ttCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTcc                                                                                                                                           >  1:808935/1‑151 (MQ=255)
       cACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAg                                                                                                                                         <  1:755293/151‑1 (MQ=255)
          gcgcTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGttt                                                                                                                                      >  2:508509/1‑151 (MQ=255)
              tGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATg                                                                                                                                  >  1:415039/1‑151 (MQ=255)
                cGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGAAAGCTTCTTCCAGTTTGATGGt                                                                                                                                <  1:294773/151‑1 (MQ=255)
                             cGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTg                                                                                                                    <  2:893920/150‑1 (MQ=255)
                                   gCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAgg                                                                                                             <  2:17587/151‑1 (MQ=255)
                                   gCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAgg                                                                                                             >  2:808639/1‑151 (MQ=255)
                                     cTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGcc                                                                                                           >  1:269206/1‑151 (MQ=255)
                                          gCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGaa                                                                                                       >  2:594992/1‑150 (MQ=255)
                                            cAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtg                                                                                                    >  2:630057/1‑151 (MQ=255)
                                             aGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtgt                                                                                                   >  1:585983/1‑151 (MQ=255)
                                              gggTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtgtg                                                                                                  >  2:232067/1‑151 (MQ=255)
                                               ggTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATgtgt                                                                                                 <  2:587689/151‑1 (MQ=255)
                                                  tGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAg                                                                                               <  1:635626/150‑1 (MQ=255)
                                                    cagcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATgtgt                                                                                                 >  2:186126/1‑146 (MQ=255)
                                                        acacaTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATc                                                                                            >  2:510960/1‑147 (MQ=255)
                                                         cacaTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCg                                                                                       <  1:453260/151‑1 (MQ=255)
                                                         cacaTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCg                                                                                       >  1:1030658/1‑151 (MQ=255)
                                                         cacaTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCg                                                                                       <  1:280865/151‑1 (MQ=255)
                                                         cacaTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCg                                                                                       <  2:441716/151‑1 (MQ=255)
                                                              gatgGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGAcgc                                                                                  >  2:693813/1‑151 (MQ=255)
                                                                          tGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGt                                                                       >  1:838199/1‑150 (MQ=255)
                                                                           gcgcCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTa                                                                      <  1:616427/150‑1 (MQ=255)
                                                                             gcCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACaa                                                                   <  1:762823/151‑1 (MQ=255)
                                                                              ccAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAAc                                                                  >  2:1065126/1‑151 (MQ=255)
                                                                                aaCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCg                                                                <  1:666139/151‑1 (MQ=255)
                                                                                aaCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCg                                                                >  1:905294/1‑151 (MQ=255)
                                                                                   gCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGcc                                                              >  2:503142/1‑150 (MQ=255)
                                                                                     tGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAgg                                                           >  2:457139/1‑151 (MQ=255)
                                                                                      gACTTTTTCCTTCCCAATATGTGCACGTTTCAAACAACCACCGACGGAGGCTTTACCGGAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTACAAGCCCAGAAGGGTGTAGATCAGCCGGACGCGCACCGATGGTAAAACCGCCAgg                                                           <  2:463495/150‑1 (MQ=255)
                                                                                      gACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGt                                                          <  1:992791/151‑1 (MQ=255)
                                                                                      gACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGt                                                          <  1:554783/151‑1 (MQ=255)
                                                                                      gACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGt                                                          >  1:537396/1‑151 (MQ=255)
                                                                                          ttATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGt                                                       <  1:604153/150‑1 (MQ=255)
                                                                                          ttATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTg                                                      >  1:905455/1‑151 (MQ=255)
                                                                                                aTCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACtt                                                <  1:43471/151‑1 (MQ=255)
                                                                                                  cACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGc                                              >  2:811166/1‑151 (MQ=255)
                                                                                                             cACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCaa                                    >  2:749439/1‑150 (MQ=255)
                                                                                                                cTTTCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAgcg                                >  2:860642/1‑151 (MQ=255)
                                                                                                                 tttCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAgcgc                               >  1:784187/1‑151 (MQ=255)
                                                                                                                   tCAAACGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAgcgcg                              <  2:615706/150‑1 (MQ=255)
                                                                                                                     aaaCGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCt                            >  1:230553/1‑150 (MQ=255)
                                                                                                                      aaCGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTG‑CCCTGACCAAAGCGCGCTAcc                         >  2:1053150/1‑151 (MQ=255)
                                                                                                                       aCGACCATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTAcc                         <  1:112423/151‑1 (MQ=255)
                                                                                                                            cATCGACGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCgg                    >  1:598863/1‑151 (MQ=255)
                                                                                                                                  cGGAAGCTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGcccc                >  1:899344/1‑149 (MQ=255)
                                                                                                                                        cTTTAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGccc         >  1:281645/1‑150 (MQ=255)
                                                                                                                                          ttAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAg       <  2:479344/150‑1 (MQ=255)
                                                                                                                                           tAACGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGACCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGa      <  2:1010251/150‑1 (MQ=255)
                                                                                                                                            aaCGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGCTCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGAt     <  2:966231/150‑1 (MQ=255)
                                                                                                                                              cGTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGAt     >  1:94621/1‑148 (MQ=255)
                                                                                                                                               gTAAGCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGa  >  1:948286/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGA  >  NC_000913/3335926‑3336218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A