Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F50 I1 R1
|
10 |
71.6 |
2231174 |
98.9% |
2206631 |
150.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,908,805 |
Δ2 bp |
coding (634‑635/891 nt) |
pstA ← |
phosphate ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,908,805 | 0 | G | . | 100.0%
| 232.2
/ NA
| 48 | coding (635/891 nt) | pstA | phosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (22/26); total (22/26) |
| * | NC_000913 | 3,908,806 | 0 | T | . | 100.0%
| 232.2
/ NA
| 48 | coding (634/891 nt) | pstA | phosphate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (22/26); total (22/26) |
CGCAAACTTAAAGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCGGTCATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTTCTCGGTGGTGCGGATAACAATCGGCAC > NC_000913/3908669‑3908947
||
cGCAAACTTAAAGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGaca < 1:894880/150‑1 (MQ=255)
aaaCTTAAAGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCg > 2:914087/1‑150 (MQ=255)
aaCTTAAAGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCCGCAGGATACCG‑‑CATAATCCCGGACACCGAc < 2:606783/151‑1 (MQ=255)
aaaGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCttt < 1:940125/151‑1 (MQ=255)
aaaGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCttt > 2:180120/1‑151 (MQ=255)
aaGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTc < 1:335348/151‑1 (MQ=255)
gATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTc > 2:689536/1‑149 (MQ=255)
gATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTc > 1:353618/1‑149 (MQ=255)
cACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAAt > 2:453798/1‑151 (MQ=255)
aCCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATc < 2:24862/151‑1 (MQ=255)
cAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCaga < 1:180128/151‑1 (MQ=255)
aGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCa > 1:974642/1‑148 (MQ=255)
gTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGat < 2:630907/151‑1 (MQ=255)
ttGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCag > 1:72745/1‑146 (MQ=255)
gCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGatcatc > 2:883813/1‑151 (MQ=255)
cGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCt > 1:327311/1‑151 (MQ=255)
gATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCtt > 1:939296/1‑151 (MQ=255)
gATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCtt > 1:291813/1‑151 (MQ=255)
tCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTcc > 2:89021/1‑151 (MQ=255)
tCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTc < 2:923981/150‑1 (MQ=255)
cGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTcc < 1:826612/150‑1 (MQ=255)
cGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTcc > 1:25290/1‑150 (MQ=255)
cGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCa < 1:53017/151‑1 (MQ=255)
gCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACt > 2:669231/1‑151 (MQ=255)
gCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACtt < 2:72386/149‑1 (MQ=255)
gCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACtt < 2:1045185/149‑1 (MQ=255)
gCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCg > 1:551348/1‑151 (MQ=255)
catcatGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCgg < 2:415834/151‑1 (MQ=255)
atGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGgtgt > 1:741004/1‑150 (MQ=255)
ccGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCa < 2:664872/151‑1 (MQ=255)
cGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAg < 1:228843/151‑1 (MQ=255)
gAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATaa > 2:52342/1‑149 (MQ=255)
tGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCt > 1:199355/1‑151 (MQ=255)
gagaGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGc < 1:851479/151‑1 (MQ=255)
gagCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAg < 1:548538/151‑1 (MQ=255)
gagCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAg < 1:883647/151‑1 (MQ=255)
cgcgGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCt < 1:631618/151‑1 (MQ=255)
aaaCAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACg < 1:749187/150‑1 (MQ=255)
ggcgCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTc < 1:1059243/151‑1 (MQ=255)
gcgGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGc < 1:6691/151‑1 (MQ=255)
cgGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGc > 2:728319/1‑150 (MQ=255)
tCACCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTtc > 2:736310/1‑151 (MQ=255)
aCCAGCAATACGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTtctc < 1:567960/151‑1 (MQ=255)
cGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTTCTCGGTGGTGCgg < 2:668051/151‑1 (MQ=255)
cGGGCAATCGCCAGCAGGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTTCTCGGTGGTGCgg > 1:952884/1‑151 (MQ=255)
aaTCGCCAGCAGGAGACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTGCAGCATGTTCTCGGTGGTGCGGATaa < 2:347585/150‑1 (MQ=255)
agcagGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTTCTCGGTGGTGCGGATAACAATCGGc < 1:296056/151‑1 (MQ=255)
cagGATACCG‑‑CATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTTCTCGGTGGTGCGGATAACAATCGGCAc > 1:740716/1‑151 (MQ=255)
||
CGCAAACTTAAAGATCGTCACCGGCAGGTTGGCGATCGGCTGCATCATGTCCGTGCTCCAGAACTGGTTGGAGAGCGCGGTAAACAGCAGCGGCGCGGTTTCACCAGCAATACGGGCAATCGCCAGCAGGATACCGGTCATAATCCCGGACACCGACGCTTTCAGCGTAATCGCAGAGATCATCTTCCACTTCGGTGTACCCAGCGCATAAGCCGCTTCACGCAGGCTGTACGGCACCAGTTTCAGCATGTTCTCGGTGGTGCGGATAACAATCGGCAC > NC_000913/3908669‑3908947
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
