Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F50 I3 R1 105 107.9 3491495 95.1% 3320411 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,793,850 (C)6→7 coding (260/552 nt) btuE ← glutathione peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,793,8441.C98.9% 342.9 / ‑3.3 90coding (266/552 nt)btuEglutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (37/52);  total (37/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATAT  >  NC_000913/1793698‑1793984
                                                                                                                                                    |                                                                                                                                            
gCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑cccc                                                                                                                                          >  4:446460/1‑151 (MQ=255)
 cGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑ccccc                                                                                                                                         >  2:807850/1‑151 (MQ=255)
  ggTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑cccccc                                                                                                                                        >  2:1126452/1‑151 (MQ=255)
         ggCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATgtgg                                                                                                                                  <  2:255843/151‑1 (MQ=255)
          gCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATgtggt                                                                                                                                 <  1:251256/151‑1 (MQ=255)
              ttGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGtggtgg                                                                                                                               <  2:580820/149‑1 (MQ=255)
              ttGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTggtggt                                                                                                                              >  2:835162/1‑150 (MQ=255)
               tGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTa                                                                                                                             <  1:501494/150‑1 (MQ=255)
                gCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTAc                                                                                                                            <  1:206101/150‑1 (MQ=255)
                   gACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGt                                                                                                                         <  2:476762/150‑1 (MQ=255)
                   gACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTa                                                                                                                        <  2:303458/151‑1 (MQ=255)
                       aTACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGtt                                                                                                                    <  1:1131823/151‑1 (MQ=255)
                         aCGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGtttt                                                                                                                  <  3:117056/151‑1 (MQ=255)
                          cGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGtttt                                                                                                                  >  1:854032/1‑150 (MQ=255)
                           gggCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTaa                                                                                                                <  2:842519/151‑1 (MQ=255)
                           gggCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTaa                                                                                                                <  2:653234/151‑1 (MQ=255)
                             gCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAAtc                                                                                                              <  2:454465/151‑1 (MQ=255)
                              cATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAAtct                                                                                                             <  1:1195563/151‑1 (MQ=255)
                              cATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAAtct                                                                                                             <  1:940636/151‑1 (MQ=255)
                                 aGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATctct                                                                                                           <  3:254962/150‑1 (MQ=255)
                                 aGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATctct                                                                                                           <  3:50614/150‑1 (MQ=255)
                                   aaTCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCa                                                                                                        <  4:365955/151‑1 (MQ=255)
                                    aTCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCAt                                                                                                       <  1:399697/151‑1 (MQ=255)
                                      ccGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCg                                                                                                     <  2:199996/151‑1 (MQ=255)
                                      ccGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCg                                                                                                     <  3:161208/151‑1 (MQ=255)
                                      ccGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCg                                                                                                     <  4:433893/151‑1 (MQ=255)
                                       cGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGc                                                                                                    <  1:955516/151‑1 (MQ=255)
                                            tctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGccc                                                                                               >  1:707068/1‑151 (MQ=255)
                                            tctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGccc                                                                                               >  1:167325/1‑151 (MQ=255)
                                             ctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGcc                                                                                                <  1:376427/149‑1 (MQ=255)
                                             ctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGc                                                                                                 <  1:389263/148‑1 (MQ=255)
                                               tCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCg                                                                                              <  4:24151/149‑1 (MQ=255)
                                                ccGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGt                                                                                            <  4:260508/150‑1 (MQ=255)
                                                     gcgACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGt                                                                                      >  2:426450/1‑151 (MQ=255)
                                                        aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTcc                                                                                   >  1:1065834/1‑151 (MQ=255)
                                                                cGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACt                                                                            <  1:359090/150‑1 (MQ=255)
                                                                 ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTgg                                                                          >  1:584782/1‑151 (MQ=255)
                                                                   cgcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGtt                                                                        >  3:143486/1‑151 (MQ=255)
                                                                   cgcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGtt                                                                        <  2:160225/151‑1 (MQ=255)
                                                                    gcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTg                                                                       >  2:362669/1‑151 (MQ=255)
                                                                      tGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGc                                                                      >  1:880524/1‑150 (MQ=255)
                                                                      tGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGc                                                                      >  4:270581/1‑150 (MQ=255)
                                                                       gCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGc                                                                      <  1:877161/149‑1 (MQ=255)
                                                                        cGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACg                                                                   <  1:444907/151‑1 (MQ=255)
                                                                        cGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACg                                                                   >  4:6358/1‑151 (MQ=255)
                                                                           caatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGa                                                                <  1:447368/151‑1 (MQ=255)
                                                                            aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGaa                                                               <  3:248184/151‑1 (MQ=255)
                                                                            aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGaa                                                               <  1:924724/151‑1 (MQ=255)
                                                                             atcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAAt                                                              >  4:196903/1‑151 (MQ=255)
                                                                              tcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATc                                                             >  2:1267020/1‑151 (MQ=255)
                                                                               caatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATcc                                                            <  3:69832/151‑1 (MQ=255)
                                                                                    tttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGc                                                        >  2:495724/1‑150 (MQ=255)
                                                                                      tGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCAc                                                      <  1:847849/150‑1 (MQ=255)
                                                                                       gATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCAcc                                                     >  2:578082/1‑150 (MQ=255)
                                                                                       gATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCAcc                                                     >  4:354154/1‑150 (MQ=255)
                                                                                           cAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaa                                                <  1:616654/151‑1 (MQ=255)
                                                                                           cAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaa                                                 >  2:642859/1‑150 (MQ=255)
                                                                                            aGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                               <  2:1265109/151‑1 (MQ=255)
                                                                                            aGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                               <  2:1102173/151‑1 (MQ=255)
                                                                                            aGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                               <  2:579892/151‑1 (MQ=255)
                                                                                              cGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAAcc                                             <  2:685185/151‑1 (MQ=255)
                                                                                               ggATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAAcc                                             >  2:232312/1‑150 (MQ=255)
                                                                                               ggATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCt                                            >  2:623162/1‑151 (MQ=255)
                                                                                                gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtc                                           <  2:711853/151‑1 (MQ=255)
                                                                                                gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtc                                           <  2:796664/151‑1 (MQ=255)
                                                                                                 aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                          <  1:621830/151‑1 (MQ=255)
                                                                                                 aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                          >  2:113215/1‑151 (MQ=255)
                                                                                                   ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                        >  1:522011/1‑151 (MQ=255)
                                                                                                   ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcga                                         <  1:1270703/150‑1 (MQ=255)
                                                                                                    gCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatc                                       <  3:143066/151‑1 (MQ=255)
                                                                                                     cGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatcg                                      <  3:114592/151‑1 (MQ=255)
                                                                                                       tCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGAc                                    >  3:411314/1‑151 (MQ=255)
                                                                                                            cGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAgg                               >  2:578219/1‑151 (MQ=255)
                                                                                                            cGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAg                                <  2:706923/150‑1 (MQ=255)
                                                                                                              ccATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCt                             <  1:143864/151‑1 (MQ=255)
                                                                                                              ccATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCt                             >  1:986912/1‑151 (MQ=255)
                                                                                                              caaTTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGAGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCt                             >  3:352321/3‑151 (MQ=255)
                                                                                                               cATTAACTTCAATCTTACTGAACATCGGGAACGTCTC‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCCCCATAAAACCTCGATCGACCCAGGCtt                            <  2:1051525/151‑1 (MQ=255)
                                                                                                                   aaCTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTg                        >  1:248208/1‑151 (MQ=255)
                                                                                                                      ttCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAat                     >  3:163519/1‑151 (MQ=255)
                                                                                                                       tCAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa                      <  2:133424/149‑1 (MQ=255)
                                                                                                                        cAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAatat                   >  1:1101736/1‑151 (MQ=255)
                                                                                                                        cAATCTTACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAata                    <  4:405147/150‑1 (MQ=255)
                                                                                                                             ttACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc              >  4:121007/1‑151 (MQ=255)
                                                                                                                             ttACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc              >  2:769059/1‑151 (MQ=255)
                                                                                                                              tACTGAACATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc              <  2:974532/150‑1 (MQ=255)
                                                                                                                                    aCATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt       >  3:321255/1‑151 (MQ=255)
                                                                                                                                    aCATCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt       >  2:243196/1‑151 (MQ=255)
                                                                                                                                      aTCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTc      >  2:147353/1‑150 (MQ=255)
                                                                                                                                      aTCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa     <  3:272367/151‑1 (MQ=255)
                                                                                                                                       tCGGGAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat    >  2:1280246/1‑151 (MQ=255)
                                                                                                                                         gggAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCatat  >  2:311291/1‑151 (MQ=255)
                                                                                                                                         gggAACGTC‑ACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCatat  >  2:1048307/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATAT  >  NC_000913/1793698‑1793984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A