Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I2 R1 150 68.2 2132745 98.7% 2105019 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,224,823 A→G A16A (GCT→GCC)  pbpG ← D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,224,8230AG98.6% 268.7 / NA 73A16A (GCT→GCC) pbpGD‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (32/40);  minor base C (0/1);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGA  >  NC_000913/2224676‑2224970
                                                                                                                                                   |                                                                                                                                                   
aaTCGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCAcggcc                                                                                                                                                  >  1:670267/1‑151 (MQ=255)
     gACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCAt                                                                                                                                             <  2:237597/151‑1 (MQ=255)
      aCGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATc                                                                                                                                            >  2:787099/1‑151 (MQ=255)
      aCGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATc                                                                                                                                            <  1:689482/151‑1 (MQ=255)
        gCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATc                                                                                                                                            >  1:161059/1‑149 (MQ=255)
             aGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGcc                                                                                                                                      >  2:653359/1‑150 (MQ=255)
             aGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCa                                                                                                                                     <  1:605599/151‑1 (MQ=255)
               aTCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAgg                                                                                                                                   >  2:298953/1‑151 (MQ=255)
               aTCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAgg                                                                                                                                   >  2:263211/1‑151 (MQ=255)
                 ccGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCt                                                                                                                                 <  2:1018237/151‑1 (MQ=255)
                       ggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaataa                                                                                                                           <  1:1033082/151‑1 (MQ=255)
                         ttCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaataa                                                                                                                           >  2:159537/1‑149 (MQ=255)
                         ttCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaataa                                                                                                                           >  2:340683/1‑149 (MQ=255)
                          tCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAg                                                                                                                         <  1:816812/150‑1 (MQ=255)
                          tCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAg                                                                                                                         <  1:610061/150‑1 (MQ=255)
                                aGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCg                                                                                                                  <  2:562529/151‑1 (MQ=255)
                                  aTCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGaa                                                                                                                <  1:340694/151‑1 (MQ=255)
                                  aTCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGa                                                                                                                 >  2:328076/1‑150 (MQ=255)
                                    cACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGaaa                                                                                                               <  1:1019755/150‑1 (MQ=255)
                                    cACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAAt                                                                                                              >  1:275357/1‑151 (MQ=255)
                                     aCTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAAtt                                                                                                             >  2:178563/1‑151 (MQ=255)
                                       tttGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTc                                                                                                           >  2:457518/1‑151 (MQ=255)
                                        ttgttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTc                                                                                                           <  2:483863/150‑1 (MQ=255)
                                         tgttgGTATGCAGATCAACAAACATCGCGCTAACGGAGGCAATTGCCGGTTGTGAGGCGGTGGAAGCGGCTGCCGTTTTCGCAACGGCCTTGGGTGCAAAAGGCGCGGCCAGCAGCAGGGCCAGGCTAAATAAAGAAACGCGAAAttt                                                                                                            <  2:471595/148‑1 (MQ=255)
                                          gttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCgg                                                                                                         <  1:1008083/150‑1 (MQ=255)
                                             ggTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGTCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCat                                                                                                      <  2:754346/150‑1 (MQ=255)
                                             ggTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCatg                                                                                                     <  1:59930/151‑1 (MQ=255)
                                             ggTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCatg                                                                                                     <  1:14435/151‑1 (MQ=255)
                                                   cAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAg                                                                                                <  1:681519/150‑1 (MQ=255)
                                                   cAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAg                                                                                                <  1:423273/150‑1 (MQ=255)
                                                    aGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCa                                                                                              <  1:73464/151‑1 (MQ=255)
                                                     gATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCAt                                                                                             >  1:371568/1‑151 (MQ=255)
                                                        caacaaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTc                                                                                           <  1:729567/150‑1 (MQ=255)
                                                        caacaaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCa                                                                                          <  1:185286/151‑1 (MQ=255)
                                                         aacaaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTGCGGCATGATGAGCATTCAg                                                                                         <  1:426949/151‑1 (MQ=255)
                                                          acaaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTc                                                                                           >  2:319258/1‑148 (MQ=255)
                                                            aaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATa                                                                                      <  1:966091/151‑1 (MQ=255)
                                                             atcatcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGAt                                                                                       >  1:991166/1‑149 (MQ=255)
                                                               catcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTg                                                                                   >  2:274740/1‑151 (MQ=255)
                                                                atcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTgg                                                                                  >  2:843740/1‑151 (MQ=255)
                                                                atcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTgg                                                                                  >  2:100847/1‑151 (MQ=255)
                                                                  cgcgCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGtt                                                                                >  1:729731/1‑151 (MQ=255)
                                                                         ccGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCAcg                                                                            <  2:692552/148‑1 (MQ=255)
                                                                           ggAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCa                                                                        <  1:363455/150‑1 (MQ=255)
                                                                                 aaTTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTg                                                                 >  2:978909/1‑151 (MQ=255)
                                                                                 aaTTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTg                                                                 <  2:455087/151‑1 (MQ=255)
                                                                                 aaTTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTg                                                                 >  2:750677/1‑151 (MQ=255)
                                                                                     tCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTgcgc                                                              <  1:688683/150‑1 (MQ=255)
                                                                                        ggTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCAccg                                                          >  1:767305/1‑151 (MQ=255)
                                                                                          ttGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCAccgcc                                                        >  2:442583/1‑151 (MQ=255)
                                                                                              gAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCgg                                                      >  1:296345/1‑149 (MQ=255)
                                                                                                   ggtggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAgg                                               >  1:304274/1‑151 (MQ=255)
                                                                                                    gtggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAgg                                               >  1:796765/1‑150 (MQ=255)
                                                                                                      ggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGAtt                                            >  1:471001/1‑151 (MQ=255)
                                                                                                      ggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGAt                                             <  1:319268/150‑1 (MQ=255)
                                                                                                           cGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTAc                                         <  2:1050491/149‑1 (MQ=255)
                                                                                                           cGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTg                                       <  2:531714/151‑1 (MQ=255)
                                                                                                             gCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAg                                     <  1:349956/151‑1 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGc                                   <  2:441362/150‑1 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCt                                  >  2:309309/1‑151 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCt                                  >  2:1036096/1‑151 (MQ=255)
                                                                                                                   gTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGc                               <  1:159542/151‑1 (MQ=255)
                                                                                                                     tttCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCg                              >  2:973454/1‑150 (MQ=255)
                                                                                                                         gCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGcc                         <  1:944371/151‑1 (MQ=255)
                                                                                                                             cGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatc                      >  2:55438/1‑150 (MQ=255)
                                                                                                                                   gCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACg                <  2:1031046/150‑1 (MQ=255)
                                                                                                                                   gCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACg                >  2:907199/1‑150 (MQ=255)
                                                                                                                                   gCGGTGCAAAAGGCACCGCCAGCATCAAGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGGCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAAc                 <  1:87874/149‑1 (MQ=255)
                                                                                                                                      gTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCa            <  1:900275/151‑1 (MQ=255)
                                                                                                                                        gCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaa          <  1:658373/151‑1 (MQ=255)
                                                                                                                                        gCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaa           <  1:445259/150‑1 (MQ=255)
                                                                                                                                        gCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaa           <  2:1001821/150‑1 (MQ=255)
                                                                                                                                            aaGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGt      >  1:320054/1‑151 (MQ=255)
                                                                                                                                             aGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg     <  1:979969/151‑1 (MQ=255)
                                                                                                                                                cacgGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGa  <  2:532480/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATCGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGA  >  NC_000913/2224676‑2224970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A