Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	144	141.8	4677362	93.6%	4378010	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,888,023	(A)_8→9	intergenic (‑32/+38)	rnd ← / ← fadD	ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,888,015	1	.	A	96.2%	505.5 / 9.9	130	intergenic (‑24/+46)	rnd/fadD	ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand): ref base . (2/3); new base A (64/61); total (66/64)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 142 total aligned reads displayed.

CCCCAGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCC  >  NC_000913/1887866‑1888157

 cccAGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑a                                                                                                                                                >  1:940152/1‑150 (MQ=255)

  ccAGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa                                                                                                                                              <  3:515766/151‑1 (MQ=255)

  ccAGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aa                                                                                                                                               >  2:1182474/1‑150 (MQ=255)

    aGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa‑a                                                                                                                                            >  2:11443/1‑150 (MQ=255)

    aGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa‑a                                                                                                                                            >  1:361828/1‑150 (MQ=255)

    aGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGACCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa                                                                                                                                              >  3:347756/1‑149 (MQ=255)

     gCTGCGGGTAATTAGTGCGCGTACGAACAAATTCAGTATCCAGGGCAATCGCCGGAACGGCACGGTCGGCTTCACACAAAGAAGCCAGCGCATCGGCCGTGGTAAGCATTTGGTAATCCAAATTGTTTGCTCTATCGTGGGCGTC‑‑aaa                                                                                                                                              <  1:700676/148‑1 (MQ=255)

       tGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa‑aaaa                                                                                                                                         >  2:502146/1‑150 (MQ=255)

        gCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa‑aaaa                                                                                                                                         <  1:52319/149‑1 (MQ=255)

          gggTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa‑aaaaaacg                                                                                                                                     >  1:753925/1‑148 (MQ=255)

          gggTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑aaa‑aaaaaac                                                                                                                                      <  1:740816/150‑3 (MQ=255)

           ggTAATAAGTGCGCGCACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑AAC‑AAAAACGcc                                                                                                                                    <  1:349974/151‑1 (MQ=255)

             taataaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCg                                                                                                                                   >  2:466440/1‑151 (MQ=255)

              aataaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCgg                                                                                                                                  >  2:193593/1‑151 (MQ=255)

              aataaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCgg                                                                                                                                  >  2:933851/1‑151 (MQ=255)

              aataaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCgg                                                                                                                                  >  2:1690152/1‑151 (MQ=255)

                 aaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGAtt                                                                                                                               >  3:332128/1‑151 (MQ=255)

                  aGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTa                                                                                                                              <  2:1668359/151‑1 (MQ=255)

                   gTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTaa                                                                                                                             <  2:260866/151‑1 (MQ=255)

                    tGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAAc                                                                                                                            >  1:451917/1‑151 (MQ=255)

                      cgcgTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCg                                                                                                                          >  1:199673/1‑151 (MQ=255)

                      cgcgTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCg                                                                                                                          >  1:292376/1‑151 (MQ=255)

                      cgcgTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCg                                                                                                                          >  3:396811/1‑151 (MQ=255)

                        cgTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGc                                                                                                                        >  2:911429/1‑151 (MQ=255)

                        cgTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGc                                                                                                                        <  1:740140/151‑1 (MQ=255)

                         gTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCg                                                                                                                       <  1:38710/151‑1 (MQ=255)

                         gTACGAACAAATGCAGTATCAAGGGCTATCGCCGGAAAGGAACGGACGGCTTCACACAAAGAAGCCAGCGCATGGTCCGTGGAAATAATTGGGTAATTCAAATTGTTTTCTCTTTAGTGGGGGTCA‑AAA‑AAAAACGCCGGATTAACCg                                                                                                                          <  4:444380/148‑1 (MQ=255)

                               aCAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTgac                                                                                                                 >  2:419774/1‑151 (MQ=255)

                                cAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTgacg                                                                                                                >  3:18352/1‑151 (MQ=255)

                                    ttCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACgactg                                                                                                            >  3:553260/1‑151 (MQ=255)

                                          aTCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTaa                                                                                                      <  2:982991/151‑1 (MQ=255)

                                            ccAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACg                                                                                                    <  2:37566/151‑1 (MQ=255)

                                             cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                   <  1:380403/151‑1 (MQ=255)

                                             cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                   <  4:505844/151‑1 (MQ=255)

                                              aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCt                                                                                                  >  1:1691576/1‑151 (MQ=255)

                                              aGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCt                                                                                                  <  2:1274843/151‑1 (MQ=255)

                                                   tATCGCCGGAAAGGCACGGACGGCTTCACACAAAGCAGCCAGCGCATCGTACGCGGCAATAATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGc                                                                                             >  1:515507/1‑151 (MQ=255)

                                                     tCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCtt                                                                                           >  1:453458/1‑151 (MQ=255)

                                                     tCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCtt                                                                                           >  2:1343581/1‑151 (MQ=255)

                                                      cGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCttt                                                                                          <  4:428089/151‑1 (MQ=255)

                                                       gtttgattggCACGAACGGGTGAACGCAAAGAAGATACGGCCTCGTCCGTGGTAATCATTTGGTAATTCAAACTGTTTTCTCTTTAGTCTGCGTC‑‑AAAGAACAACGCCGGATTAACCTGCGTCTGTTGACTGACTTATCGCTCAGGCttt                                                                                          <  1:1479074/142‑1 (MQ=255)

                                                       gCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCttt                                                                                          >  1:602233/1‑150 (MQ=255)

                                                           gAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGt                                                                                     <  2:1412713/151‑1 (MQ=255)

                                                           gAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGt                                                                                     <  4:570460/151‑1 (MQ=255)

                                                                    ggacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGc                                                                            <  1:843684/151‑1 (MQ=255)

                                                                      acggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcg                                                                          >  1:609700/1‑151 (MQ=255)

                                                                        ggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcg                                                                        >  4:147177/1‑151 (MQ=255)

                                                                        ggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcg                                                                        <  2:675545/151‑1 (MQ=255)

                                                                          cTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgcg                                                                      >  2:502787/1‑151 (MQ=255)

                                                                             cacacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCtt                                                                   >  3:215360/1‑151 (MQ=255)

                                                                             cacacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCtt                                                                   <  1:996273/151‑1 (MQ=255)

                                                                             cacacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCt                                                                    <  2:794787/150‑1 (MQ=255)

                                                                              acacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                  <  2:294873/151‑1 (MQ=255)

                                                                              acacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                  <  2:1697655/151‑1 (MQ=255)

                                                                                acaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGt                                                                <  2:844545/151‑1 (MQ=255)

                                                                                 caAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGt                                                                <  1:148935/150‑1 (MQ=255)

                                                                                 caAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTc                                                               >  3:318504/1‑151 (MQ=255)

                                                                                       agCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTa                                                          >  2:1654877/1‑150 (MQ=255)

                                                                                         ccAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAAtt                                                       >  1:162996/1‑151 (MQ=255)

                                                                                         ccAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAAtt                                                       <  1:130492/151‑1 (MQ=255)

                                                                                         ccAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAAtt                                                       <  2:1155148/151‑1 (MQ=255)

                                                                                            gcgcATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATtctc                                                    <  1:1527173/151‑1 (MQ=255)

                                                                                            gcgcATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATtctc                                                    <  4:153296/151‑1 (MQ=255)

                                                                                            gcgcATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATtctc                                                    >  4:479077/1‑151 (MQ=255)

                                                                                             cgcATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcg                                                   <  2:396466/151‑1 (MQ=255)

                                                                                               cATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                 <  1:272009/151‑1 (MQ=255)

                                                                                                aTCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtcg                                                <  1:1000469/151‑1 (MQ=255)

                                                                                                  cGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGc                                               >  2:1111371/1‑150 (MQ=255)

                                                                                                    tCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCaa                                             >  2:1341224/1‑150 (MQ=255)

                                                                                                    tCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCaa                                             >  1:1520979/1‑150 (MQ=255)

                                                                                                     ccGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCaaa                                            <  1:1066772/150‑1 (MQ=255)

                                                                                                          gTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                      <  2:1129403/151‑1 (MQ=255)

                                                                                                           tAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                     >  1:1711099/1‑151 (MQ=255)

                                                                                                            aaTCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTcc                                    >  3:427390/1‑151 (MQ=255)

                                                                                                            aaTCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTcc                                    <  2:1292637/151‑1 (MQ=255)

                                                                                                            aaTCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTc                                     <  1:217645/150‑1 (MQ=255)

                                                                                                              tCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                 >  1:339283/1‑151 (MQ=255)

                                                                                                                aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACg                                >  1:1336537/1‑151 (MQ=255)

                                                                                                                 tttGGGAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGt                               >  2:33451/1‑151 (MQ=255)

                                                                                                                   tGGTAATTCAAATTGTTTTCTCTTTAGTGGGTGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGtt                              <  3:225523/150‑1 (MQ=255)

                                                                                                                   tGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGtt                              >  1:855777/1‑150 (MQ=255)

                                                                                                                    ggTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTa                             <  2:100506/150‑1 (MQ=255)

                                                                                                                      tAATTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCCCTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGtt                              >  1:1211405/1‑147 (MQ=255)

                                                                                                                       aaTTCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGAt                          <  4:560275/150‑1 (MQ=255)

                                                                                                                          tCAAATTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTc                       <  3:45256/150‑1 (MQ=255)

                                                                                                                            aaaTTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGt                    >  3:487888/1‑151 (MQ=255)

                                                                                                                            aaaTTGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGt                    >  2:769160/1‑151 (MQ=255)

                                                                                                                                tGTTTTCTCTTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACt                >  2:1518756/1‑151 (MQ=255)

                                                                                                                                    ttCTCTTTAGTGGGCGACA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTTACGACTGACTTAACGATCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGCTAGATTTCGGTAACtcatc            >  1:1127297/1‑151 (MQ=255)

                                                                                                                                      ctctTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc          >  2:340489/1‑151 (MQ=255)

                                                                                                                                      ctctTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc          >  2:1217234/1‑151 (MQ=255)

                                                                                                                                       tctTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACg         >  2:1231967/1‑151 (MQ=255)

                                                                                                                                        ctTTAGTGGGCGTCAAAAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca           <  2:1554657/149‑1 (MQ=255)

                                                                                                                                        ctTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca           <  1:11441/148‑1 (MQ=255)

                                                                                                                                        ctTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca           <  1:1023536/148‑1 (MQ=255)

                                                                                                                                        ctTTAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACg         >  3:231849/1‑150 (MQ=255)

                                                                                                                                         tttAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGa        >  2:365785/1‑150 (MQ=255)

                                                                                                                                           tAGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAAc     >  4:338335/1‑151 (MQ=255)

                                                                                                                                            aGTGGGCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACt    <  2:354877/151‑1 (MQ=255)

                                                                                                                                               gggCGTCA‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcc  >  3:183514/1‑150 (MQ=255)

CCCCAGCTGCGGGTAATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTC‑‑AAA‑AAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCC  >  NC_000913/1887866‑1888157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A