Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I3 R1	143	109.6	3434427	98.5%	3382910	150.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,458,429	T→C	Y244C (TAT→TGT)	fadJ ←	enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,458,429	0	T	C	99.0%	374.6 / ‑3.9	103	Y244C (TAT→TGT)	fadJ	enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (50/52); total (51/52)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

Only 100 of 103 total aligned reads displayed.

CGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCG  >  NC_000913/2458281‑2458569

cGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACaa                                                                                                                                            <  1:1358597/151‑1 (MQ=255)

         aaaaaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTg                                                                                                                                    >  2:1326392/1‑150 (MQ=255)

         aaaaaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTg                                                                                                                                    >  1:1309139/1‑150 (MQ=255)

          aaaaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTg                                                                                                                                    >  1:889736/1‑149 (MQ=255)

          aaaaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTg                                                                                                                                    >  2:1436604/1‑149 (MQ=255)

            aaaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGtt                                                                                                                                <  2:721128/151‑1 (MQ=255)

             aaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGttt                                                                                                                               >  2:1693001/1‑151 (MQ=255)

             aaGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGttt                                                                                                                               >  1:1479014/1‑151 (MQ=255)

              aGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGtttt                                                                                                                              >  2:1142129/1‑151 (MQ=255)

               gATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGAGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGtttt                                                                                                                              >  1:750260/1‑150 (MQ=255)

                aTACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTtgt                                                                                                                            >  1:11459/1‑151 (MQ=255)

                  actacGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTtgtg                                                                                                                           <  1:1227410/150‑1 (MQ=255)

                    tacGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTgtgt                                                                                                                          >  2:1308258/1‑149 (MQ=255)

                    tacGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTc                                                                                                                        >  1:1334696/1‑151 (MQ=255)

                     acGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTc                                                                                                                        <  1:239833/150‑1 (MQ=255)

                     acGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCt                                                                                                                       >  2:1188631/1‑151 (MQ=255)

                       gCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTg                                                                                                                      <  2:282222/150‑1 (MQ=255)

                        cAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGtt                                                                                                                    >  1:623349/1‑151 (MQ=255)

                         aGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGttt                                                                                                                   >  1:1183567/1‑151 (MQ=255)

                          gcgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGtttt                                                                                                                  <  2:681333/151‑1 (MQ=255)

                           cgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTc                                                                                                                 >  2:1336837/1‑151 (MQ=255)

                           cgcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTc                                                                                                                 <  2:131352/151‑1 (MQ=255)

                            gcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCt                                                                                                                <  1:308223/151‑1 (MQ=255)

                            gcCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCt                                                                                                                >  1:88113/1‑151 (MQ=255)

                             ccTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCtt                                                                                                               <  1:1632103/151‑1 (MQ=255)

                              cTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTg                                                                                                              >  2:1308499/1‑151 (MQ=255)

                               tGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGc                                                                                                             >  2:53529/1‑151 (MQ=255)

                               tGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCACTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGc                                                                                                             >  1:1317789/1‑151 (MQ=255)

                                gCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGcc                                                                                                            >  2:1580168/1‑151 (MQ=255)

                                 cGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCg                                                                                                           <  2:1631753/151‑1 (MQ=255)

                                  gATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCg                                                                                                           >  1:1223067/1‑150 (MQ=255)

                                  gATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGa                                                                                                          <  1:634022/151‑1 (MQ=255)

                                   aTTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGAc                                                                                                         <  1:948216/151‑1 (MQ=255)

                                   aTTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGAc                                                                                                         >  1:386979/1‑151 (MQ=255)

                                     tgtgGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGAcc                                                                                                        >  1:201645/1‑150 (MQ=255)

                                      gtgGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCAt                                                                                                      <  1:482330/151‑1 (MQ=255)

                                          cGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTg                                                                                                  <  1:416292/151‑1 (MQ=255)

                                          cGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTg                                                                                                  >  1:1273343/1‑151 (MQ=255)

                                          cGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTg                                                                                                  >  2:504089/1‑151 (MQ=255)

                                          cGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTg                                                                                                  >  2:406898/1‑151 (MQ=255)

                                           gTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTg                                                                                                  >  1:1083928/1‑150 (MQ=255)

                                             cATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAAc                                                                                               <  2:1188116/151‑1 (MQ=255)

                                             cATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTAAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGaa                                                                                                <  2:1267487/150‑1 (MQ=255)

                                                cGCCAGTTCGCCAAACGCCCGAGTTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAAcag                                                                                             <  1:757644/150‑1 (MQ=255)

                                                cGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAAcagc                                                                                            >  1:379628/1‑151 (MQ=255)

                                                 gCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAAcagca                                                                                           <  2:232543/151‑1 (MQ=255)

                                                  ccAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAAcagc                                                                                            >  1:755389/1‑149 (MQ=255)

                                                   cAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACagcagc                                                                                         >  2:1479430/1‑151 (MQ=255)

                                                        cGCCAAACGCCCGAGCTTCGGCGTCATACCCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTGTTGTGTTCTGTTTTCTTGCCGACCATTGTGAACAGCAGCGCa                                                                                      <  1:220130/149‑1 (MQ=255)

                                                        cGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACg                                                                                    >  1:1350974/1‑151 (MQ=255)

                                                         gCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGa                                                                                   <  2:222904/151‑1 (MQ=255)

                                                              aCGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTaa                                                                              <  2:1551744/151‑1 (MQ=255)

                                                               cGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTaa                                                                              >  2:1498922/1‑150 (MQ=255)

                                                                  ccGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGc                                                                          <  1:1336743/151‑1 (MQ=255)

                                                                   cGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGcc                                                                         <  2:1418306/151‑1 (MQ=255)

                                                                   cGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGcc                                                                         >  1:917860/1‑151 (MQ=255)

                                                                      gCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGcccc                                                                       <  1:1681614/150‑1 (MQ=255)

                                                                           ggCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGa                                                                 <  2:883736/151‑1 (MQ=255)

                                                                           ggCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGa                                                                 <  2:1630303/151‑1 (MQ=255)

                                                                             cGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAAt                                                               <  2:53675/151‑1 (MQ=255)

                                                                              gTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAAt                                                               <  2:1457892/150‑1 (MQ=255)

                                                                              gTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATa                                                              <  2:1608331/151‑1 (MQ=255)

                                                                                cATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACg                                                            >  2:1515365/1‑151 (MQ=255)

                                                                                cATAACCGCTGCTGGTGCCCTGCCCTAATCCCGTTTCAACACCCTCCAGAATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGGCAGAAt                                                               <  1:1690721/148‑1 (MQ=255)

                                                                                 aTAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGCCCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACg                                                            <  1:260154/150‑1 (MQ=255)

                                                                                 aTAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGCCCTAACGGCCCCGCCAGAATAc                                                             <  1:2674/149‑1 (MQ=255)

                                                                                   agccGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACACCCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGCCCATTTTGAACAGCAGCGCACGACCTAAGGGCCCCGCCAGAATACGCt                                                          <  1:622982/148‑1 (MQ=255)

                                                                                    aCCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTcg                                                        >  1:764636/1‑151 (MQ=255)

                                                                                     ccGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTcgc                                                       <  2:251101/151‑1 (MQ=255)

                                                                                          gctgGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTAc                                                   <  2:868777/150‑1 (MQ=255)

                                                                                          gctgGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACa                                                  <  2:1089715/151‑1 (MQ=255)

                                                                                            tgGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAgg                                                >  2:48136/1‑151 (MQ=255)

                                                                                             ggTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTTTTTTCTTGCCGACCATTTTGAAAAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTCCAGGt                                               <  1:1308164/151‑1 (MQ=255)

                                                                                             ggTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGt                                               <  2:35191/151‑1 (MQ=255)

                                                                                             ggTGCCCTGCGCTAACCCCGTTTCAACACCCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGt                                               <  1:1289279/151‑1 (MQ=255)

                                                                                                gCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTaga                                            <  2:57863/151‑1 (MQ=255)

                                                                                                 cccTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTag                                             >  2:754951/1‑149 (MQ=255)

                                                                                                  ccggcgtTAATCCCGTTTCATCATCCTCCAGGATGCGTTCTTTCGCCGGACAATTTCCTTGCGTTTTGTGTTCTGTTTTCTTGCCGACGATTTTAAACAGCACCGCGCGCTCAAACGGCCCCTCCAGAATACGCTCGCGTACAGGTAGAgg                                          <  1:503329/148‑1 (MQ=255)

                                                                                                    tGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGc                                        >  2:893360/1‑151 (MQ=255)

                                                                                                        cTAATCCCTTTTCAACACCCCCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCggg                                     <  2:890202/150‑1 (MQ=255)

                                                                                                          aaTCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAg                                  <  2:828334/151‑1 (MQ=255)

                                                                                                             cccGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCggg                                     <  1:332531/145‑1 (MQ=255)

                                                                                                             cccGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTGGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATg                               <  1:1236054/151‑1 (MQ=255)

                                                                                                               cGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCCCGCGAACGGGAAGAGGGGGGAAAGATggg                             >  2:611589/1‑151 (MQ=255)

                                                                                                                        aacCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttc                    >  2:1298011/1‑151 (MQ=255)

                                                                                                                        aacCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCctt                     >  1:1127477/1‑150 (MQ=255)

                                                                                                                         acCTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttct                   >  2:1531604/1‑151 (MQ=255)

                                                                                                                          ccTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCcttctt                  <  2:1084014/151‑1 (MQ=255)

                                                                                                                           cTCCAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCttt                 >  1:1082247/1‑151 (MQ=255)

                                                                                                                             ccAGTATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCttt                 <  1:745482/149‑1 (MQ=255)

                                                                                                                              cAGGATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGcc              >  1:494204/1‑151 (MQ=255)

                                                                                                                                ggATGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCa             <  2:1116942/150‑1 (MQ=255)

                                                                                                                                  aTGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGGCCATTTGGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGc           <  2:1317884/150‑1 (MQ=255)

                                                                                                                                   tGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCt          <  1:458741/150‑1 (MQ=255)

                                                                                                                                   tGCGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTc         >  1:1377412/1‑151 (MQ=255)

                                                                                                                                     cGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCa        >  1:1625603/1‑150 (MQ=255)

                                                                                                                                     cGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCa        >  1:552032/1‑150 (MQ=255)

                                                                                                                                     cGTTCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCa        >  1:508487/1‑150 (MQ=255)

                                                                                                                                       ttCTGTCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAAc      >  1:275751/1‑150 (MQ=255)

                                                                                                                                            tCGCCGGACAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCg  <  2:127862/149‑1 (MQ=255)

CGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAACCGCTGCTGGTGCCCTGCGCTAATCCCGTTTCAACAACCTCCAGGATGCGTTCTGTCGCCGGATAATTGCCTTGAGTTTTGTGTTCTGTTTTCTTGCCGACCATTTTGAACAGCAGCGCACGACCTAACGGCCCCGCCAGAATACGCTCGCGTACAGGTAGAGGGCGGGAAGATGGGCGCTCCTTCTTTGCCAGCTCAACAGCG  >  NC_000913/2458281‑2458569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A