Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I1 R1	141	76.7	2402765	98.4%	2364320	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,353,790	G→A	A382T (GCC→ACC)	glpA →	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,353,790	0	G	A	100.0%	250.1 / NA	63	A382T (GCC→ACC)	glpA	anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/30); total (33/30)

TGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGT  >  NC_000913/2353650‑2353936

tgctCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGc                                                                                                                                          >  1:769770/1‑151 (MQ=255)

   tCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACt                                                                                                                                       >  1:679169/1‑151 (MQ=255)

         aTGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTgg                                                                                                                                 <  1:829389/151‑1 (MQ=255)

           gCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGtt                                                                                                                               <  2:769781/151‑1 (MQ=255)

               aaCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTcaca                                                                                                                           <  2:745862/151‑1 (MQ=255)

                 cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAg                                                                                                                         <  1:232175/151‑1 (MQ=255)

                  gcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTcaca                                                                                                                           >  1:971846/1‑148 (MQ=255)

                   cgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGaa                                                                                                                       <  1:24575/151‑1 (MQ=255)

                     aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAAcc                                                                                                                     <  2:620766/151‑1 (MQ=255)

                            ggACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCt                                                                                                                 <  2:265632/148‑1 (MQ=255)

                                ggATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGtt                                                                                                           >  1:47906/1‑150 (MQ=255)

                                    ttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCtt                                                                                                      >  1:1033854/1‑151 (MQ=255)

                                    ttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCt                                                                                                       <  1:1068317/150‑1 (MQ=255)

                                     tATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCtt                                                                                                      >  2:839214/1‑150 (MQ=255)

                                     tATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTg                                                                                                     >  1:1012564/1‑151 (MQ=255)

                                      atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAATTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTg                                                                                                     <  1:1034604/150‑1 (MQ=255)

                                      atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTg                                                                                                     <  2:535167/150‑1 (MQ=255)

                                      atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGc                                                                                                    >  2:334983/1‑151 (MQ=255)

                                        caccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGt                                                                                                  <  1:1145055/151‑1 (MQ=255)

                                          ccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTaa                                                                                                <  2:18562/151‑1 (MQ=255)

                                              caccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTc                                                                                            >  1:597212/1‑151 (MQ=255)

                                              caccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGACACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTc                                                                                            >  2:319015/1‑151 (MQ=255)

                                                     ggCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTccc                                                                                     <  1:884027/151‑1 (MQ=255)

                                                     ggCAAACTAAGGACTAACCGGCTGATGCTTGAATGGGTTCCCGACCGGGTTTGCCGCAAACT‑GGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcct                                                                                    <  1:578262/151‑1 (MQ=255)

                                                       cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctg                                                                                   >  1:763100/1‑151 (MQ=255)

                                                        aaaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgc                                                                                  <  1:1169892/151‑1 (MQ=255)

                                                        aaaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgc                                                                                  >  1:889514/1‑151 (MQ=255)

                                                         aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgcc                                                                                 >  1:1113588/1‑151 (MQ=255)

                                                         aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCcctgcc                                                                                 <  1:1169061/151‑1 (MQ=255)

                                                             gatgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCtgcctgcc                                                                             >  1:1086472/1‑151 (MQ=255)

                                                             gatgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCtgcctgcc                                                                             >  1:1118017/1‑151 (MQ=255)

                                                                gaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGcccc                                                                           <  1:14842/150‑1 (MQ=255)

                                                                     aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTgcg                                                                     >  2:778860/1‑151 (MQ=255)

                                                                       cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGcgcg                                                                   <  1:558600/151‑1 (MQ=255)

                                                                         gCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGTTCTGGCACTGCCTGCTTCACAAAAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGt                                                                 <  1:218737/151‑1 (MQ=255)

                                                                            gATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCt                                                              >  1:403989/1‑151 (MQ=255)

                                                                             aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCt                                                              <  2:277975/150‑1 (MQ=255)

                                                                             aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCt                                                              >  1:277972/1‑150 (MQ=255)

                                                                              tGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGc                                                            >  2:553811/1‑151 (MQ=255)

                                                                                       gggCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATc                                                    <  2:143233/150‑1 (MQ=255)

                                                                                           tACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCAt                                               >  1:1049570/1‑151 (MQ=255)

                                                                                            aCCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATg                                              <  2:87190/151‑1 (MQ=255)

                                                                                              cGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGc                                            >  2:294508/1‑151 (MQ=255)

                                                                                               gACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCg                                           >  1:1052922/1‑151 (MQ=255)

                                                                                                 cgcgGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGAt                                         <  1:240959/151‑1 (MQ=255)

                                                                                                  gcgGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATc                                        >  2:1174668/1‑151 (MQ=255)

                                                                                                       aTGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACg                                   >  2:159076/1‑151 (MQ=255)

                                                                                                       aTGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACg                                   <  2:1134886/151‑1 (MQ=255)

                                                                                                            gCAAACTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACgcctgc                              >  2:868323/1‑151 (MQ=255)

                                                                                                              aaaCTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACgcctgcct                            >  1:231577/1‑151 (MQ=255)

                                                                                                               aaCTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGcctgcctg                           >  1:942858/1‑151 (MQ=255)

                                                                                                               aaCTGGGCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGcctgcctg                           <  1:777156/151‑1 (MQ=255)

                                                                                                                     gCAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCGCGCCTGCCTGGCTg                       >  2:356991/1‑149 (MQ=255)

                                                                                                                      cAACACGCGCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGc                    <  1:790568/151‑1 (MQ=255)

                                                                                                                           cgcgCCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAgg               >  2:274305/1‑151 (MQ=255)

                                                                                                                              gcCCCTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCg            >  2:1050382/1‑151 (MQ=255)

                                                                                                                               ccccTGTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGt           <  1:755013/151‑1 (MQ=255)

                                                                                                                               ccccTGTACGACTACCGATCGGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGt           <  2:56056/151‑1 (MQ=255)

                                                                                                                                    gTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGc       >  1:244724/1‑150 (MQ=255)

                                                                                                                                    gTACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGc       >  2:778441/1‑150 (MQ=255)

                                                                                                                                     tACGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCAc     >  1:978806/1‑151 (MQ=255)

                                                                                                                                       cGACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCg   <  1:335405/151‑1 (MQ=255)

                                                                                                                                        gACTACCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGt  <  2:63527/151‑1 (MQ=255)

TGCTCGACCATGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTCATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCGTCTGCACCGT  >  NC_000913/2353650‑2353936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A