Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I2 R1 121 82.0 2593086 97.7% 2533445 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,249,386 T→C E124E (GAA→GAG)  dhaL ← dihydroxyacetone kinase, C‑terminal domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,249,3860TC98.7% 260.3 / ‑3.7 74E124E (GAA→GAG) dhaLdihydroxyacetone kinase, C‑terminal domain
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (33/40);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CATCGTAATCGTACTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCACCACCGAC  >  NC_000913/1249242‑1249532
                                                                                                                                                |                                                                                                                                                  
cATCGTAATCGTACTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCt                                                                                                                                              <  2:386120/151‑1 (MQ=255)
 atcgtaatcgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCtt                                                                                                                                             <  2:445668/151‑1 (MQ=255)
     taatcgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTccc                                                                                                                                         >  2:430694/1‑151 (MQ=255)
      aatcgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTccc                                                                                                                                         <  2:307243/150‑1 (MQ=255)
        tcgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCcgcg                                                                                                                                      >  1:233375/1‑151 (MQ=255)
         cgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGa                                                                                                                                     <  2:981835/151‑1 (MQ=255)
         cgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCcgcg                                                                                                                                      <  1:684773/150‑1 (MQ=255)
         cgtaCTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCcgcg                                                                                                                                      <  2:418482/150‑1 (MQ=255)
                 gtgCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTa                                                                                                                              <  2:464779/150‑1 (MQ=255)
                  tgCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTAc                                                                                                                             <  2:274581/150‑1 (MQ=255)
                    cAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGcc                                                                                                                          <  2:749691/151‑1 (MQ=255)
                     aGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCg                                                                                                                         >  1:1076685/1‑151 (MQ=255)
                       cGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTc                                                                                                                       <  1:787584/151‑1 (MQ=255)
                          aTTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCcgc                                                                                                                    <  1:436887/151‑1 (MQ=255)
                             cGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGcc                                                                                                                 <  1:1004496/151‑1 (MQ=255)
                                cGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATc                                                                                                              <  2:1024957/151‑1 (MQ=255)
                                 gATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcg                                                                                                             <  1:382731/151‑1 (MQ=255)
                                    gCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                                           <  2:254571/150‑1 (MQ=255)
                                     cTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                                          >  1:1178458/1‑150 (MQ=255)
                                      tACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                                          >  2:843895/1‑149 (MQ=255)
                                       aCTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAAc                                                                                                       <  2:549876/151‑1 (MQ=255)
                                         tGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACAt                                                                                                     >  1:768766/1‑151 (MQ=255)
                                         tGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACAt                                                                                                     >  2:77901/1‑151 (MQ=255)
                                          ggCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACa                                                                                                      <  2:911653/149‑1 (MQ=255)
                                            cAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTg                                                                                                  >  2:472336/1‑151 (MQ=255)
                                             aGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTg                                                                                                  >  2:171085/1‑150 (MQ=255)
                                              gCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGAt                                                                                                <  1:751644/151‑1 (MQ=255)
                                                 tCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATaa                                                                                              <  1:111307/150‑1 (MQ=255)
                                                  cGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGTTaaa                                                                                             <  2:737796/150‑1 (MQ=255)
                                                   gAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCCCCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGc                                                                                           <  2:536708/151‑1 (MQ=255)
                                                    aGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGct                                                                                          >  1:748867/1‑151 (MQ=255)
                                                        ccACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                                      >  1:525029/1‑151 (MQ=255)
                                                         cACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTcc                                                                                     <  2:963604/151‑1 (MQ=255)
                                                         cACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTcc                                                                                     >  1:1144622/1‑151 (MQ=255)
                                                         cACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTcc                                                                                     >  1:628278/1‑151 (MQ=255)
                                                           ccGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCa                                                                                    <  2:957252/150‑1 (MQ=255)
                                                            cGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgt                                                                                  >  1:358112/1‑151 (MQ=255)
                                                            cGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgt                                                                                  <  2:1237/151‑1 (MQ=255)
                                                             ggAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtg                                                                                 >  1:858487/1‑151 (MQ=255)
                                                             ggAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtg                                                                                 <  1:306058/151‑1 (MQ=255)
                                                              gAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtgt                                                                                <  2:131590/151‑1 (MQ=255)
                                                              gAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtgt                                                                                >  2:195018/1‑151 (MQ=255)
                                                                 cAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTc                                                                               >  2:1089820/1‑149 (MQ=255)
                                                                       gaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTg                                                                       <  1:715976/151‑1 (MQ=255)
                                                                            tGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGt                                                                   <  1:431302/150‑1 (MQ=255)
                                                                             gCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTg                                                                  <  2:816381/150‑1 (MQ=255)
                                                                                cGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGTTAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGcc                                                                >  1:1069295/1‑149 (MQ=255)
                                                                                cGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTg                                                              >  1:398145/1‑151 (MQ=255)
                                                                                  cTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTACAGTGTCAGGCTTTGCCGTGACTggg                                                            >  2:155917/1‑151 (MQ=255)
                                                                                    ggACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTc                                                          <  2:289705/151‑1 (MQ=255)
                                                                                    ggACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTc                                                          <  2:862908/151‑1 (MQ=255)
                                                                                     gACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCg                                                         >  2:859820/1‑151 (MQ=255)
                                                                                         gACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCt                                                      <  2:844662/150‑1 (MQ=255)
                                                                                              aaCGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcgg                                                <  2:326399/151‑1 (MQ=255)
                                                                                               aCGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggc                                               <  1:172822/151‑1 (MQ=255)
                                                                                                  aTTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGcgcg                                            >  1:1023306/1‑151 (MQ=255)
                                                                                                     ccaccaCCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGAt                                         <  2:350727/151‑1 (MQ=255)
                                                                                                     ccaccaCCGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGAt                                         <  2:1040509/151‑1 (MQ=255)
                                                                                                           ccGGCTCCCACACATCTCACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGTGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAaagaa                                   <  1:44861/151‑1 (MQ=255)
                                                                                                           ccGGCACCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGCGCCTGGGTCGCCTGGGCGGCGCGGATAaaga                                    >  1:163005/1‑150 (MQ=255)
                                                                                                                aCCCACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTAc                              >  1:672672/1‑151 (MQ=255)
                                                                                                                 cccACACATCACACATGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTAcc                             >  2:550471/1‑151 (MQ=255)
                                                                                                                     cacatcacacatGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAAc                         >  2:1291320/1‑151 (MQ=255)
                                                                                                                     cacatcacacatGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAAc                         >  1:331142/1‑151 (MQ=255)
                                                                                                                      acatcacacatGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACa                        <  2:99185/151‑1 (MQ=255)
                                                                                                                          cacacatGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCg                     <  2:233378/150‑1 (MQ=255)
                                                                                                                           acacatGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCgg                    >  1:1280138/1‑150 (MQ=255)
                                                                                                                           acacatGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCg                     <  1:1206504/149‑1 (MQ=255)
                                                                                                                               atGGTTTTATCGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCAc               >  1:1201819/1‑151 (MQ=255)
                                                                                                                                         cGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCaccacc     >  1:27967/1‑151 (MQ=255)
                                                                                                                                         cGCCAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCaccacc     >  2:946933/1‑151 (MQ=255)
                                                                                                                                           ccAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCACCACCGa   <  1:925403/151‑1 (MQ=255)
                                                                                                                                            cAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCACCACCGAc  >  2:29384/1‑151 (MQ=255)
                                                                                                                                            cAGGCTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCACCACCGAc  >  2:84950/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CATCGTAATCGTACTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGACCACTGGCACCACCGAC  >  NC_000913/1249242‑1249532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A