Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I2 R1 121 82.0 2593086 97.7% 2533445 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,442,318 A→G Y176H (TAT→CAT)  ldhA ← fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,442,3180AG98.9% 329.0 / NA 87Y176H (TAT→CAT) ldhAfermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (39/47);  minor base T (0/1);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGT  >  NC_000913/1442171‑1442465
                                                                                                                                                   |                                                                                                                                                   
ttttCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCg                                                                                                                                                   <  1:376410/150‑1 (MQ=255)
  ttCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGa                                                                                                                                                 <  2:204818/150‑1 (MQ=255)
   tCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATc                                                                                                                                               >  2:677308/1‑151 (MQ=255)
    cATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCg                                                                                                                                              >  1:76614/1‑151 (MQ=255)
     aTCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCg                                                                                                                                              >  1:95376/1‑150 (MQ=255)
        tGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACg                                                                                                                                          >  2:405753/1‑151 (MQ=255)
         gTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGa                                                                                                                                             <  2:750172/147‑1 (MQ=255)
          ttCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGcc                                                                                                                                        >  2:666596/1‑151 (MQ=255)
            cGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCca                                                                                                                                       <  2:296386/150‑1 (MQ=255)
             gAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCcagc                                                                                                                                     >  1:271999/1‑151 (MQ=255)
             gAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCcagc                                                                                                                                     >  2:195756/1‑151 (MQ=255)
              aaGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCcagc                                                                                                                                     >  2:231817/1‑150 (MQ=255)
              aaGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCcagc                                                                                                                                     >  2:66827/1‑150 (MQ=255)
                 gcggcTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGAc                                                                                                                                 <  2:272009/151‑1 (MQ=255)
                  cggcTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACg                                                                                                                                <  1:407979/151‑1 (MQ=255)
                   ggcTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGc                                                                                                                               >  2:792344/1‑151 (MQ=255)
                   ggcTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGc                                                                                                                               <  1:1040825/151‑1 (MQ=255)
                       tCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATa                                                                                                                            <  1:647054/150‑1 (MQ=255)
                       tCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATa                                                                                                                            <  1:419016/150‑1 (MQ=255)
                       tCGTTCAACAGATGAAAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATACCGTCTGCTTCATAGACCAGGGTTGGCAGATCGACATACCCCACACCGAGTTCCAGGGCCGCTGCACTCAGATGCGGAACGTACGCCAGCAGACGCATa                                                                                                                            <  1:904219/150‑1 (MQ=255)
                            cAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCaaaa                                                                                                                      <  2:450061/151‑1 (MQ=255)
                            cAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCaaaa                                                                                                                      <  1:619053/151‑1 (MQ=255)
                            cAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCaaa                                                                                                                       <  1:553344/150‑1 (MQ=255)
                              aCAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAAcc                                                                                                                    <  2:725527/151‑1 (MQ=255)
                                 gatgatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCttt                                                                                                                 >  2:1213971/1‑151 (MQ=255)
                                   tgatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTc                                                                                                                >  1:253989/1‑150 (MQ=255)
                                    gatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCa                                                                                                               <  1:36896/150‑1 (MQ=255)
                                    gatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAg                                                                                                              <  1:221987/151‑1 (MQ=255)
                                    gatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAg                                                                                                              <  2:306479/151‑1 (MQ=255)
                                       aGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAAt                                                                                                           <  1:148891/151‑1 (MQ=255)
                                         ttttCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATgc                                                                                                         >  2:515479/1‑151 (MQ=255)
                                          tttCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATgc                                                                                                         <  1:644033/150‑1 (MQ=255)
                                           ttCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATgcgc                                                                                                       >  1:477763/1‑151 (MQ=255)
                                              cGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCgcagc                                                                                                    <  2:258657/151‑1 (MQ=255)
                                               ggTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGAACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCgcagc                                                                                                    >  1:174386/1‑150 (MQ=255)
                                                    cAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGcc                                                                                              >  2:663165/1‑151 (MQ=255)
                                                           cAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACGCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAACATCGCCACAcc                                                                                         <  1:954314/149‑1 (MQ=255)
                                                               gCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGAttt                                                                                    <  1:152395/150‑1 (MQ=255)
                                                                cAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGAtttt                                                                                   >  2:307913/1‑150 (MQ=255)
                                                                 agagagATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTAc                                                                                 <  1:324905/151‑1 (MQ=255)
                                                                         aaCGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTAcc                                                                          >  1:450965/1‑150 (MQ=255)
                                                                         aaCGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTAcc                                                                          >  1:929536/1‑150 (MQ=255)
                                                                           cGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGa                                                                        <  1:859615/150‑1 (MQ=255)
                                                                            gTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGAt                                                                       >  1:396406/1‑150 (MQ=255)
                                                                            gTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATa                                                                      <  2:79355/151‑1 (MQ=255)
                                                                             tCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATa                                                                      >  2:412761/1‑150 (MQ=255)
                                                                               tGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACg                                                                   >  2:1011302/1‑151 (MQ=255)
                                                                                 aTTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGcc                                                                 >  2:980181/1‑151 (MQ=255)
                                                                                  ttCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCt                                                                >  1:834140/1‑151 (MQ=255)
                                                                                   tCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTg                                                               >  1:1232448/1‑151 (MQ=255)
                                                                                     atagaACAGGGTTGGCAAATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACTCCGATTTTACCGGTACCGATAACGCCTGc                                                              <  1:1056208/148‑1 (MQ=255)
                                                                                      gagaACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGc                                                              >  1:743505/1‑149 (MQ=255)
                                                                                           cAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTg                                                       <  2:1270981/151‑1 (MQ=255)
                                                                                              ggTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGcc                                                     >  1:460704/1‑150 (MQ=255)
                                                                                               gTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcat                                                   >  2:91887/1‑151 (MQ=255)
                                                                                                  ggCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcataca                                                <  1:52351/151‑1 (MQ=255)
                                                                                                   gCAGATCGACATACTCCACACCGAGTTCCAGCTCCGCTCCGCTTGGATTGGTAACGACCGCCTGCAAACGCATACCAAAACCTTACAGAAGGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTGTTACcata                                                  <  1:573910/148‑1 (MQ=255)
                                                                                                   gCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcatacat                                               <  2:1029560/151‑1 (MQ=255)
                                                                                                     aGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcatacat                                               >  1:644550/1‑149 (MQ=255)
                                                                                                     aGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcatacat                                               >  2:221619/1‑149 (MQ=255)
                                                                                                     aGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcatacat                                               >  2:496156/1‑149 (MQ=255)
                                                                                                     aGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCcatacat                                               >  2:571748/1‑149 (MQ=255)
                                                                                                      gATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAg                                             >  1:1166988/1‑150 (MQ=255)
                                                                                                      gATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGt                                            <  2:1254320/151‑1 (MQ=255)
                                                                                                       aTCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTa                                           <  2:654443/151‑1 (MQ=255)
                                                                                                          gACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAg                                        <  1:187454/151‑1 (MQ=255)
                                                                                                           aCATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAGCGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGc                                       <  2:1162497/151‑1 (MQ=255)
                                                                                                             aTACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCg                                     <  1:84649/151‑1 (MQ=255)
                                                                                                             aTACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCg                                     <  2:1166689/151‑1 (MQ=255)
                                                                                                               aCTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGt                                   <  1:231814/151‑1 (MQ=255)
                                                                                                                  ccACACCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCa                                 <  2:9584/150‑1 (MQ=255)
                                                                                                                     cacCGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGAcc                             >  2:966878/1‑151 (MQ=255)
                                                                                                                       ccGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCtt                           <  2:120945/151‑1 (MQ=255)
                                                                                                                       ccGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCt                            <  2:1263108/150‑1 (MQ=255)
                                                                                                                        cGAGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTc                          >  2:1111010/1‑151 (MQ=255)
                                                                                                                          aGTTCCAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTcc                         >  1:522358/1‑150 (MQ=255)
                                                                                                                              ccAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCagaga                    <  1:752940/151‑1 (MQ=255)
                                                                                                                              ccAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCagaga                    <  1:179594/151‑1 (MQ=255)
                                                                                                                              ccAGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCagag                     <  1:715651/150‑1 (MQ=255)
                                                                                                                                aGCGCCGCTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCaga                      >  1:874612/1‑147 (MQ=255)
                                                                                                                                   gccgcTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCGGAGAGAAGt               <  2:295977/151‑1 (MQ=255)
                                                                                                                                    ccgcTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGtt              <  2:253997/151‑1 (MQ=255)
                                                                                                                                     cgcTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTa             <  1:953124/151‑1 (MQ=255)
                                                                                                                                      gcTGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAg            <  2:15307/151‑1 (MQ=255)
                                                                                                                                        tGCACTTGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGc           >  2:858637/1‑150 (MQ=255)
                                                                                                                                             ttGGATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACg     >  2:1055572/1‑151 (MQ=255)
                                                                                                                                                gATGCGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGt  <  2:928864/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATAACGCCTGCCGTTTTGCCATACATAGTAAAGCCGGTCAGACCTTCCAGAGAGAAGTTAGCATCACGGGT  >  NC_000913/1442171‑1442465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A