Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I2 R1	121	82.0	2593086	97.7%	2533445	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,473,880	C→T	R93C (CGC→TGC)	yfdP →	CPS‑53 (KpLE1) prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,473,880	0	C	T	100.0%	263.9 / NA	68	R93C (CGC→TGC)	yfdP	CPS‑53 (KpLE1) prophage; uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (40/28); total (40/28)

AAGGTCTCACTCAACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTGAGGACT  >  NC_000913/2473733‑2474027

aaggtCTCACTCAACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGc                                                                                                                                                   <  2:488831/150‑1 (MQ=255)

     ctcactcaACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACt                                                                                                                                             >  2:13417/1‑151 (MQ=255)

       cactcaACCTGGAAATCTAACCATTTGATGAGAACCGTCTGAAAATCAACCACAAACTCTAATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACCCCCCCGAAACGCCGATGTATGTCAATCTCGGTGGTTGCCTGACTag                                                                                                                                           <  2:123195/151‑2 (MQ=255)

       cactcaACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTGAATCGCGGTGGTTGCCTGACTAt                                                                                                                                           >  1:126039/1‑151 (MQ=255)

       cactcaACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGAc                                                                                                                                              <  1:579000/148‑1 (MQ=255)

       cactcaACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTAt                                                                                                                                           >  2:906924/1‑151 (MQ=255)

        actcaACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTAtt                                                                                                                                          >  1:42366/1‑151 (MQ=255)

            aaCCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGc                                                                                                                                      <  1:698254/151‑1 (MQ=255)

               cTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAgg                                                                                                                                   >  1:519426/1‑151 (MQ=255)

               cTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAgg                                                                                                                                   >  2:320536/1‑151 (MQ=255)

               cTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAgg                                                                                                                                   >  2:1146918/1‑151 (MQ=255)

                   aaaTCGAACCATTTGATGAGAGCCGTCTGAAAATCAAGCACAATCACTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGGCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAg                                                                                                                                <  1:406839/150‑1 (MQ=255)

                          aCCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAggg                                                                                                                         >  2:10665/1‑150 (MQ=255)

                           ccATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGAc                                                                                                                       <  1:619544/151‑1 (MQ=255)

                             aTTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACaa                                                                                                                     <  1:405889/151‑1 (MQ=255)

                                 gatgaGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTa                                                                                                                  <  2:436596/150‑1 (MQ=255)

                                        aCCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGActct                                                                                                          <  1:456053/151‑1 (MQ=255)

                                        aCCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGActct                                                                                                          <  2:309050/151‑1 (MQ=255)

                                        aCCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGActct                                                                                                          >  2:818656/1‑151 (MQ=255)

                                            tCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGcc                                                                                                      >  1:1283650/1‑151 (MQ=255)

                                                 aaaTCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGa                                                                                                 <  2:984911/151‑1 (MQ=255)

                                                  aaTCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTg                                                                                                  >  2:125840/1‑149 (MQ=255)

                                                  aaTCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGCGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTg                                                                                                  >  2:396930/1‑149 (MQ=255)

                                                           caAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACgg                                                                                        >  1:1140199/1‑150 (MQ=255)

                                                            aaaCTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGaa                                                                                      <  2:978547/151‑1 (MQ=255)

                                                            aaaCTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGa                                                                                       >  1:493252/1‑150 (MQ=255)

                                                             aaCTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGaa                                                                                      >  2:473539/1‑150 (MQ=255)

                                                              aCTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGaaa                                                                                     >  1:1238307/1‑150 (MQ=255)

                                                                  tcATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTcc                                                                                >  2:693267/1‑151 (MQ=255)

                                                                    atatGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCcgc                                                                              <  2:400597/151‑1 (MQ=255)

                                                                     tatGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCACTCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCTTGACGGAAAACTCcgcg                                                                             >  1:338297/1‑151 (MQ=255)

                                                                       tGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCgcgc                                                                            >  1:445472/1‑150 (MQ=255)

                                                                       tGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCgcgc                                                                            >  1:662104/1‑150 (MQ=255)

                                                                         ttCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCgcagc                                                                         >  1:1097011/1‑151 (MQ=255)

                                                                           cGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGcagcag                                                                       <  1:274234/151‑1 (MQ=255)

                                                                            gCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAgg                                                                      >  2:50506/1‑151 (MQ=255)

                                                                              ccGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTc                                                                    >  2:1023437/1‑151 (MQ=255)

                                                                                 aCTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCAtt                                                                 >  2:209008/1‑151 (MQ=255)

                                                                                   tAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGATTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTa                                                                >  1:894589/1‑150 (MQ=255)

                                                                                    aaCCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTaa                                                               <  1:390495/150‑1 (MQ=255)

                                                                                     aCCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAat                                                              >  2:118653/1‑150 (MQ=255)

                                                                                      ccGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAatat                                                            >  1:245929/1‑151 (MQ=255)

                                                                                       cGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATc                                                           >  2:856500/1‑151 (MQ=255)

                                                                                           ggAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTc                                                       >  1:358324/1‑151 (MQ=255)

                                                                                           ggAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTc                                                       <  2:645896/151‑1 (MQ=255)

                                                                                            gAAAATTTCCGACGAAGACACCGGCGAAACGGCGATCGATGACAGTCGCGGAGGTTGCCTGACTAATCGTCAGGAAGACGATAGAGAATTACTGACTCTTGCCGGTGAGGCTGACGGAAAGCACCGCGCAGCAGGTCATCAATATCAtt                                                        <  1:932776/149‑1 (MQ=255)

                                                                                            gAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCGGCGCAGCAGGTCATTAATATCATTCt                                                      >  2:208286/1‑151 (MQ=255)

                                                                                              aaaTTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCtt                                                     >  2:856157/1‑150 (MQ=255)

                                                                                               aaTTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCtta                                                    >  2:215575/1‑150 (MQ=255)

                                                                                                          aagaCACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAatta                                        <  1:849872/151‑1 (MQ=255)

                                                                                                           agaCACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAattat                                       <  1:19974/151‑1 (MQ=255)

                                                                                                                ccaccGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAAttatttat                                   <  1:690723/150‑1 (MQ=255)

                                                                                                                 caccGAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTAtct                                 >  1:288242/1‑151 (MQ=255)

                                                                                                                     gAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCtcatc                             >  2:154966/1‑151 (MQ=255)

                                                                                                                     gAAACGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCtcatc                             <  2:910142/151‑1 (MQ=255)

                                                                                                                      aaaCGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATAACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTCATATCATTCTTAATTAACTAATTATTTATCtcat                              >  1:843674/1‑149 (MQ=255)

                                                                                                                        aCGCCGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACt                          >  2:578343/1‑151 (MQ=255)

                                                                                                                            cGATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAat                      <  1:1288947/151‑1 (MQ=255)

                                                                                                                             gATGTATGTCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAat                      >  1:283976/1‑150 (MQ=255)

                                                                                                                               tgtatgtCAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATc                   >  1:715873/1‑151 (MQ=255)

                                                                                                                                      cAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAatat            <  2:558946/151‑1 (MQ=255)

                                                                                                                                      cAATCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAata             <  1:1158226/150‑1 (MQ=255)

                                                                                                                                       aaTCGCGGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAAtata           <  1:225713/151‑1 (MQ=255)

                                                                                                                                           gcgGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTGa       <  1:50506/151‑1 (MQ=255)

                                                                                                                                           gcgGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTGa       <  1:1220592/151‑1 (MQ=255)

                                                                                                                                            cgGTGGTTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTg        >  1:963449/1‑149 (MQ=255)

                                                                                                                                              gtggtTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTGAGGa    <  2:964235/151‑1 (MQ=255)

                                                                                                                                                ggtTGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTGAGGACt  >  2:716610/1‑151 (MQ=255)

AAGGTCTCACTCAACCTGGAAATCGAACCATTTGATGAGAACCGTCTGAAAATCAAACACAAACTCTCATATGTTCGCCCGACTAACCGTGGGAAAATTTCCGAAGAAGACACCACCGAAACGCCGATGTATGTCAATCGCGGTGGTCGCCTGACTATTCTGCAGGAAGACCAGGGACAATTACTGACTCTTGCCGGTGAGCCTGACGGAAAACTCCGCGCAGCAGGTCATTAATATCATTCTTAATTAACTAATTATTTATCTCATCACTGAATATCTTAATATAGTGAGGACT  >  NC_000913/2473733‑2474027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A