Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	551,656	A→G	R313R (CGT→CGC)	purK ←	N5‑carboxyaminoimidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	551,656	0	A	G	100.0%	231.3 / NA	62	R313R (CGT→CGC)	purK	N5‑carboxyaminoimidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/35); total (27/35)

ATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATC  >  NC_000913/551515‑551793

aTAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCcgg                                                                                                                                   >  1:441973/1‑150 (MQ=255)

     gCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCggacggac                                                                                                                             >  1:74870/1‑151 (MQ=255)

      cACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGAct                                                                                                                            <  1:1013793/151‑1 (MQ=255)

       aCCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGActt                                                                                                                           >  1:671722/1‑151 (MQ=255)

            ttAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGActtctt                                                                                                                        >  1:501745/1‑149 (MQ=255)

             tAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTg                                                                                                                      <  1:1010301/150‑1 (MQ=255)

              acccGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTACTTTGTc                                                                                                                    <  2:759448/149‑1 (MQ=255)

                ccGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCg                                                                                                                   <  2:194618/150‑1 (MQ=255)

                       tACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTg                                                                                                           >  2:62351/1‑151 (MQ=255)

                             gcgcCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAg                                                                                                        <  1:142992/148‑1 (MQ=255)

                             gcgcCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGa                                                                                                       <  1:464363/149‑1 (MQ=255)

                             gcgcCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATg                                                                                                     >  2:20550/1‑151 (MQ=255)

                                   aaaTCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCAcc                                                                                                 >  1:890980/1‑149 (MQ=255)

                                       cACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCgg                                                                                            <  1:991681/150‑1 (MQ=255)

                                        aCGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCa                                                                                          <  1:1056088/151‑1 (MQ=255)

                                         cgccgcTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAg                                                                                         <  2:500049/151‑1 (MQ=255)

                                            cgcTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGttt                                                                                      <  1:172381/151‑1 (MQ=255)

                                                ggCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCa                                                                                    <  2:750831/149‑1 (MQ=255)

                                                   atatTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAg                                                                               <  2:934397/151‑1 (MQ=255)

                                                     atTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGt                                                                              <  1:470515/150‑1 (MQ=255)

                                                     atTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTc                                                                             <  2:69889/151‑1 (MQ=255)

                                                         cggcggCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTGCAGCCAGTCATaa                                                                         <  2:594597/151‑1 (MQ=255)

                                                          ggcggcAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAAt                                                                        <  1:382343/151‑1 (MQ=255)

                                                           gcggcAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAAtt                                                                       <  1:480284/151‑1 (MQ=255)

                                                            cggcAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTc                                                                      >  1:837856/1‑151 (MQ=255)

                                                               cagcagCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTcaca                                                                   <  1:806903/151‑1 (MQ=255)

                                                               cagcagCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTcac                                                                    >  1:60022/1‑150 (MQ=255)

                                                                 gcagcGGGATTAAGGCTTCCAGCGTCGCAGTCAGACTCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACTGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACAt                                                                  <  2:249632/150‑1 (MQ=255)

                                                                       ggATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTAcc                                                           >  1:848145/1‑151 (MQ=255)

                                                                       ggATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTAcc                                                           >  2:31782/1‑151 (MQ=255)

                                                                         aTTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGCCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCa                                                          >  1:208890/1‑150 (MQ=255)

                                                                          ttAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAAt                                                        >  1:616817/1‑151 (MQ=255)

                                                                              ggCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATc                                                       >  2:115154/1‑148 (MQ=255)

                                                                                  tCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTg                                                 >  1:370318/1‑150 (MQ=255)

                                                                                   ccAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGat                                               >  2:583214/1‑151 (MQ=255)

                                                                                        gTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGatcatc                                           >  2:228023/1‑150 (MQ=255)

                                                                                        gTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGatcatc                                           >  1:309125/1‑150 (MQ=255)

                                                                                             aGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGAc                                     <  1:142796/151‑1 (MQ=255)

                                                                                             aGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGAc                                     >  1:470817/1‑151 (MQ=255)

                                                                                               tCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACgg                                   >  2:970124/1‑151 (MQ=255)

                                                                                                cAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGa                                  <  2:715853/151‑1 (MQ=255)

                                                                                                 aGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGat                                 >  1:139326/1‑151 (MQ=255)

                                                                                                 aGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGa                                  >  2:661019/1‑150 (MQ=255)

                                                                                                  gACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGatt                                <  2:806903/151‑1 (MQ=255)

                                                                                                    cgcgATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGattat                              <  2:994683/151‑1 (MQ=255)

                                                                                                        aTGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTcac                          <  2:999596/151‑1 (MQ=255)

                                                                                                           tgtCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTcaccac                       >  2:640186/1‑151 (MQ=255)

                                                                                                            gtCGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACt                      <  2:837295/151‑1 (MQ=255)

                                                                                                              cGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTgg                    <  2:537784/151‑1 (MQ=255)

                                                                                                              cGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTgg                    >  1:359474/1‑151 (MQ=255)

                                                                                                              cGCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTg                     <  2:293642/150‑1 (MQ=255)

                                                                                                               gCTGTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCCACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGt                   <  1:217851/151‑1 (MQ=255)

                                                                                                                  gTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGtt                  <  1:1015934/149‑1 (MQ=255)

                                                                                                                  gTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGtt                  <  1:516188/149‑1 (MQ=255)

                                                                                                                  gTCGGTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGc                <  1:583246/151‑1 (MQ=255)

                                                                                                                     ggTCAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGt             <  1:1015931/151‑1 (MQ=255)

                                                                                                                        cAAATTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAAc          >  1:989453/1‑151 (MQ=255)

                                                                                                                         aaaTTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACg         <  2:60021/151‑1 (MQ=255)

                                                                                                                           aTTCAGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGc       <  2:747269/151‑1 (MQ=255)

                                                                                                                               aGATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGc       >  1:521660/1‑147 (MQ=255)

                                                                                                                                gATGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATc  <  2:941176/151‑1 (MQ=255)

                                                                                                                                  tGCCCCACTTTGCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGc       >  1:89564/1‑144 (MQ=255)

ATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATC  >  NC_000913/551515‑551793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A