Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I2 R1	139	64.5	2139856	92.7%	1983646	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,640	C→T	R232C (CGC→TGC)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,640	0	C	T	100.0%	204.3 / NA	52	R232C (CGC→TGC)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/30); total (22/30)

CGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAG  >  NC_000913/1382495‑1382786

cGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCAtt                                                                                                                                               <  2:994655/151‑1 (MQ=255)

  aaaCGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTgg                                                                                                                                             >  2:1006809/1‑151 (MQ=255)

        ttGCCTTGCGAGTGAACTATTATCTCGAGCACGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTgg                                                                                                                                        >  2:357699/1‑150 (MQ=255)

           ccTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCg                                                                                                                                    >  1:38141/1‑151 (MQ=255)

           cattGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCg                                                                                                                                    >  1:38136/3‑151 (MQ=255)

            cTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGc                                                                                                                                   >  1:277895/1‑151 (MQ=255)

              tGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTg                                                                                                                                 >  2:1051616/1‑151 (MQ=255)

               gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTgg                                                                                                                                <  1:14669/151‑1 (MQ=255)

                 gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAATAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAg                                                                                                                              <  2:483113/151‑1 (MQ=255)

                 gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGa                                                                                                                               <  2:738586/150‑1 (MQ=255)

                      aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACt                                                                                                                         <  1:615955/151‑1 (MQ=255)

                          attatCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTgg                                                                                                                       <  2:941639/149‑1 (MQ=255)

                                 cGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTgg                                                                                                                 <  1:197988/148‑1 (MQ=255)

                                 cGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAg                                                                                                              <  2:810222/151‑1 (MQ=255)

                                         gCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGatat                                                                                                      <  1:144890/151‑1 (MQ=255)

                                         gCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGatat                                                                                                      <  1:402698/151‑1 (MQ=255)

                                           ttCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTg                                                                                                    >  1:120925/1‑151 (MQ=255)

                                            tCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATAtt                                                                                                     <  1:140845/149‑1 (MQ=255)

                                            tCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTg                                                                                                    <  1:564813/150‑1 (MQ=255)

                                                   ggACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGaa                                                                                            <  1:323025/151‑1 (MQ=255)

                                                   ggACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGaa                                                                                            <  2:858468/151‑1 (MQ=255)

                                                     acgacAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAgg                                                                                          >  1:228907/1‑151 (MQ=255)

                                                      cgacAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGc                                                                                         <  1:1062241/151‑1 (MQ=255)

                                                        acAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTAGGACTGGCAGGATGATATTGAACGTGAAGGcc                                                                                        >  1:140021/1‑150 (MQ=255)

                                                          aGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATg                                                                                     >  2:190691/1‑151 (MQ=255)

                                                                 aTAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTaa                                                                              <  2:280543/151‑1 (MQ=255)

                                                                 aTAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTa                                                                               >  1:897359/1‑150 (MQ=255)

                                                                  tAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAAc                                                                             >  2:817764/1‑151 (MQ=255)

                                                                    aCGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAAcca                                                                           <  2:20808/151‑2 (MQ=255)

                                                                    aCGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCg                                                                           >  1:359635/1‑151 (MQ=255)

                                                                     cGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCgt                                                                          <  1:799509/151‑1 (MQ=255)

                                                                         gAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTa                                                                       <  2:449334/150‑1 (MQ=255)

                                                                          aGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAgg                                                                     <  2:153590/151‑1 (MQ=255)

                                                                             tttCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGttt                                                                  >  2:454603/1‑151 (MQ=255)

                                                                                ccACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTAt                                                                <  1:773377/150‑1 (MQ=255)

                                                                                ccACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTAt                                                                <  2:905579/150‑1 (MQ=255)

                                                                                         aaaTTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGtt                                                       >  1:1026047/1‑150 (MQ=255)

                                                                                           aTTCACGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATg                                                    <  2:179813/151‑1 (MQ=255)

                                                                                            ttCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATg                                                    >  2:231320/1‑150 (MQ=255)

                                                                                                 gcCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTcc                                              >  2:610039/1‑151 (MQ=255)

                                                                                                     aTTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGa                                           <  1:807547/150‑1 (MQ=255)

                                                                                                      tttGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAAc                                         >  1:867260/1‑151 (MQ=255)

                                                                                                         gCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACg                                        >  2:737594/1‑149 (MQ=255)

                                                                                                            gCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACtt                                   <  1:400670/151‑1 (MQ=255)

                                                                                                                 cGCTCGGCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGt                              <  2:786887/151‑1 (MQ=255)

                                                                                                                       gCAACCACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCt                        <  1:522678/151‑1 (MQ=255)

                                                                                                                            cACTCGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAAt                   >  2:802925/1‑151 (MQ=255)

                                                                                                                                cGGTGACGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATAc                 <  1:551804/149‑1 (MQ=255)

                                                                                                                                     aCGCCGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTg          <  2:249300/151‑1 (MQ=255)

                                                                                                                                         cGTATACGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGt       >  2:322398/1‑150 (MQ=255)

                                                                                                                                             taCGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGc    >  2:241113/1‑149 (MQ=255)

                                                                                                                                              aCGTGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAg  <  1:49876/150‑1 (MQ=255)

CGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGCCGCTGACGCTCGGCAACCACTCGGTGACGCCGTATACGCGCATTGGGCTGGATCGCTGGAGTAACTGGGACTGGCAGGATGATATTGAACGTGAAGGCCATGATTTTAACCGTGTAGGTTTATTTTACGGTTATGATTTCCAGAACGGACTTTCCGTTTCGCTGGAATACGCGTTTGAGTGGCAG  >  NC_000913/1382495‑1382786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A