Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 574,257 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583574,2570AT99.6% 658.6 / NA 278intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (141/135);  total (142/136)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583574,2580CG100.0% 665.8 / NA 278intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (142/136);  total (142/136)
*CP042583574,2590TA99.6% 665.5 / NA 278intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (141/136);  minor base C (1/0);  total (142/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

TTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGAACTACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGA  >  CP042583/573979‑574536
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                     
  ttACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGCCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACGGCTTAAc                                                                                                                                                                                                                                                                             >  1:1494453/1‑289 (MQ=255)
     cGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTa                                                                                                                                                                                                                                                                          <  1:199090/289‑1 (MQ=255)
                      aTCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca                                                                                                                                                                                                                                                         <  1:1893987/289‑1 (MQ=255)
                      aTCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca                                                                                                                                                                                                                                                         >  2:832478/1‑289 (MQ=255)
                      aTCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca                                                                                                                                                                                                                                                         >  1:140350/1‑289 (MQ=255)
                        cTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaaa                                                                                                                                                                                                                                                       <  2:895968/289‑1 (MQ=255)
                        cTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaaa                                                                                                                                                                                                                                                       <  1:403197/289‑1 (MQ=255)
                         tGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGATGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCAGACGTAGCACTTTAGTCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAAGCTACCGTTCTCATCCACACAAAg                                                                                                                                                                                                                                                      >  2:891548/1‑289 (MQ=255)
                           aGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTTTTCCGAGACGTTTCTCAACGCCAATCTCACCCGCAGGATAGAGCGTTGCCATACGTAGCACTTCATCCAGAGCGATACCGCACTGTTCGACCAGATTACGAACCCCTTCAATCATGGTTAAGCATGAACCGCTTAACGTCCCCTTATCATCcacacacgct                                                                                                                                                                                                                                                     >  2:2049999/1‑284 (MQ=255)
                           aGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTtct                                                                                                                                                                                                                                                                   >  1:1039509/1‑274 (MQ=255)
                            gCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTcc                                                                                                                                                                                                                                                   >  2:1637502/1‑289 (MQ=255)
                             cTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGCACCGCTTAAGGTACCGTTCTCATCCACACAAAGTCCg                                                                                                                                                                                                                                                  >  2:716397/1‑289 (MQ=255)
                             cTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCAGCCACACAAAGTCCg                                                                                                                                                                                                                                                  >  2:1017831/1‑289 (MQ=255)
                                gTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa                                                                                                                                                                                                                                               <  2:2116119/289‑1 (MQ=255)
                                gTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa                                                                                                                                                                                                                                               <  1:1846966/289‑1 (MQ=255)
                                           gtCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGtata                                                                                                                                                                                                                                     <  1:1575712/288‑1 (MQ=255)
                                                   ttCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTa                                                                                                                                                                                                                             >  1:862483/1‑288 (MQ=255)
                                                    tctcTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTTCCGAGACTTTGCTCAACGCCAACCGCACGCGCCGGATAGAGCGTCGCCAGACGTACGACTTCATCCAGTGCGACCCCGCAATGTTCGACCAGATTAAGCACGCCTTCACTCATTGTTAAGGAGGAACCACATAACTTACCGTACTCATCCACGCAAAGTCCTGTACGTAAGAACATTAttttcc                                                                                                                                                                                                                            >  2:2143071/1‑286 (MQ=255)
                                                    tctcTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTa                                                                                                                                                                                                                                                                          <  2:70594/242‑1 (MQ=255)
                                                    tctcTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGGCCGTTACCGGAGTCTATTGTTTTAc                                                                                                                                                                                                                            >  2:1870805/1‑288 (MQ=255)
                                                           ttGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGGGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACg                                                                                                                                                                                                                                                                            <  2:229560/233‑1 (MQ=255)
                                                           ttGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACg                                                                                                                                                                                                                                                                            >  1:229560/1‑233 (MQ=255)
                                                           ttGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaaa                                                                                                                                                                                                                    <  1:2257570/289‑1 (MQ=255)
                                                              aGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGtt                                                                                                                                                                                                                                                                     <  1:1235267/237‑1 (MQ=255)
                                                                ttACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGa                                                                                                                                                                                                                >  1:2164021/1‑288 (MQ=255)
                                                                 tACGACCTCGTTACCGTTATGGTTGGTCTTGGTGATTTTAAGATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCGCCGGCGGGTGTGCCGGGACGTTTCTCAACGCCAAGCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGaa                                                                                                                                                                                                               <  2:96776/288‑1 (MQ=255)
                                                                      ccTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATTAACTGtt                                                                                                                                                                                                          <  1:53306/288‑1 (MQ=255)
                                                                      ccTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTc                                                                                                                                                                                                         <  1:832478/289‑1 (MQ=255)
                                                                       cTCGTTACCGTTAACGATGGTTTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCa                                                                                                                                                                                                        <  2:936911/289‑1 (MQ=255)
                                                                        tCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa                                                                                                                                                                                                       >  1:1168408/1‑289 (MQ=255)
                                                                             aCCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTg                                                                                                                                                                                                  >  2:1247815/1‑289 (MQ=255)
                                                                               cGTTAACGATTGTCGTGGTTATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGc                                                                                                                                                                                                <  2:1365583/289‑1 (MQ=255)
                                                                                 ttAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt                                                                                                                                                                                                                                                 <  2:583456/238‑1 (MQ=255)
                                                                                 ttAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt                                                                                                                                                                                                                                                 >  1:583456/1‑238 (MQ=255)
                                                                                 ttAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCa                                                                                                                                                                                               >  1:1392994/1‑288 (MQ=255)
                                                                                                    ttttAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTa                                                                                                                                                                                                                                                                          >  2:481739/1‑194 (MQ=255)
                                                                                                    ttttAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTc                                                                                                                                                                           >  1:301364/1‑289 (MQ=255)
                                                                                                     tttAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTtct                                                                                                                                                                                                                                                                   <  2:386418/200‑1 (MQ=255)
                                                                                                     tttAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc                                                                                                                                                                                                                                                               <  2:1052246/204‑1 (MQ=255)
                                                                                                     tttAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACgg                                                                                                                                                                                                                                            >  2:1187094/1‑223 (MQ=255)
                                                                                                     tttAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCa                                                                                                                                                                          >  1:1782858/1‑289 (MQ=255)
                                                                                                         aaaTCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTaa                                                                                                                                                                      <  1:1823529/289‑1 (MQ=255)
                                                                                                                               ggTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACg                                                                                                                                                                                                                                             <  1:617128/196‑1 (MQ=255)
                                                                                                                                 ttGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCttt                                                                                                                                                <  2:391352/287‑1 (MQ=255)
                                                                                                                                       aCTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGtt                                                                                                                                        <  2:311224/289‑1 (MQ=255)
                                                                                                                                          ttACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAg                                                                                                                                     >  1:1325865/1‑289 (MQ=255)
                                                                                                                                              cTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTACGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCACTGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGaca                                                                                                                                                               >  1:1265333/1‑259 (MQ=255)
                                                                                                                                                  ggcgAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTc                                                                                                                                                                                                         >  2:1913360/1‑213 (MQ=255)
                                                                                                                                                   gcgAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCg                                                                                                                                                     >  2:318933/1‑264 (MQ=255)
                                                                                                                                                   gcgAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGaa                                                                                                                            >  1:2124210/1‑289 (MQ=255)
                                                                                                                                                     gagtggtctgagaCGTGTCTCAACCCCCATCGCGCGCGCCGGATATAGCGTCGCCATCCGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTGAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAGTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAAt                                                                                                                           <  2:1325865/279‑1 (MQ=255)
                                                                                                                                                           gCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGc                                                                                                                     <  1:10071/288‑1 (MQ=255)
                                                                                                                                                                    tttCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTACAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACa                                                                                                           >  1:1934068/1‑289 (MQ=255)
                                                                                                                                                                      tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACAt                                                                                                          >  1:2222604/1‑288 (MQ=255)
                                                                                                                                                                      tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATg                                                                                                         <  1:954081/289‑1 (MQ=255)
                                                                                                                                                                          aaCGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTa                                                                                                                                                                                                                                                                          >  1:764752/1‑124 (MQ=255)
                                                                                                                                                                             gCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCa                                                                                                  <  2:2222604/289‑1 (MQ=255)
                                                                                                                                                                               cacgcGCACGAGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                <  2:654251/285‑1 (MQ=255)
                                                                                                                                                                               cAATCGCACGCGCCGGCTAGTGCGTAGCCATACGTAGCACTTCATCCAGGGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                <  2:1583670/289‑1 (MQ=255)
                                                                                                                                                                               cAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                <  2:543809/289‑1 (MQ=255)
                                                                                                                                                                               cAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                <  2:198548/289‑1 (MQ=255)
                                                                                                                                                                                  tCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGc                                                                                             <  2:140350/289‑1 (MQ=255)
                                                                                                                                                                                       cgcgCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataa                                                                                        >  1:181700/1‑289 (MQ=255)
                                                                                                                                                                                                        cGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATg                                                                                                         <  1:688314/255‑1 (MQ=255)
                                                                                                                                                                                                        cGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCa                                                                       >  2:74549/1‑289 (MQ=255)
                                                                                                                                                                                                           cATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCt                                                                    <  1:334394/289‑1 (MQ=255)
                                                                                                                                                                                                             tACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca                                                                                                                                                                                                                                                         >  2:1821676/1‑106 (MQ=255)
                                                                                                                                                                                                                 tAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAATATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTtcgtcg                                                              <  2:254209/289‑1 (MQ=255)
                                                                                                                                                                                                                  aGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                >  2:1787623/1‑254 (MQ=255)
                                                                                                                                                                                                                       ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCg                                                                                                                                                     <  1:2024522/196‑1 (MQ=255)
                                                                                                                                                                                                                       ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATc                                                        >  1:2092681/1‑289 (MQ=255)
                                                                                                                                                                                                                         cATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATcgc                                                      >  2:300471/1‑289 (MQ=255)
                                                                                                                                                                                                                         cATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATcg                                                       >  2:87174/1‑288 (MQ=255)
                                                                                                                                                                                                                              aGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATAGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGcgccc                                                  >  2:1685221/1‑288 (MQ=255)
                                                                                                                                                                                                                                tGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATa                                                                              >  1:315742/1‑258 (MQ=255)
                                                                                                                                                                                                                                 gCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCgcca                                              >  2:572329/1‑289 (MQ=255)
                                                                                                                                                                                                                                     tACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATg                                                                                                         >  1:1211458/1‑226 (MQ=255)
                                                                                                                                                                                                                                     tACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCgccaggcc                                          <  1:268910/289‑1 (MQ=255)
                                                                                                                                                                                                                                     tACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCgccaggcc                                          >  1:423943/1‑289 (MQ=255)
                                                                                                                                                                                                                                     tACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCgccaggcc                                          <  1:390269/289‑1 (MQ=255)
                                                                                                                                                                                                                                     tACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCcccaggcc                                          >  1:1641439/1‑289 (MQ=255)
                                                                                                                                                                                                                                              gTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACg                                 <  1:572329/289‑1 (MQ=255)
                                                                                                                                                                                                                                               ttCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGAAATAAATGTCAGCTTCCGCAAGGATCGACCCCGCCCGGGCAGGTTccaga                                >  2:2021442/1‑285 (MQ=255)
                                                                                                                                                                                                                                               ttCGACCAGATTACGCACGACTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAACAATGAACTGTTCAATTTTGGCACCTCCTGGCTTGGTGGCTTCATTAACCAGACACAGTTTGTCGCCTTTCAGACGGTCACCGTTGAGAATGTTGGCGTAATCATCATCATGGCCATCATCAAGCATACCGCAAAAAATTGTAGATGCGTCCAGTTTCGCGCCCGCCCGGCCAGGTTCatg                                 >  2:1931240/1‑286 (MQ=255)
                                                                                                                                                                                                                                                tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                               <  2:1155346/289‑1 (MQ=255)
                                                                                                                                                                                                                                                tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                               <  1:74549/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       gATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAat                        >  1:1356719/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               acgaCTTCAAGCATGGTTAAGGATGAACCGCTTAACGTACCGTTTCAATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa                <  2:1499912/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               aacacTTCAATCATGGTTAAGGATGAACCGCTTAACTTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa                <  2:2092681/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATc              <  1:71276/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGt                                                                                                                   >  1:1368085/1‑187 (MQ=255)
                                                                                                                                                                                                                                                                  ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCg                             <  2:1470979/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcg             <  1:459048/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATc              >  1:1060090/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                  ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGCTGGGGGAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTACGCTTCGGCGAGGAGTGCGCCCGCCAGGCCCGAGTCACCACCGGtccattatacaatc              >  2:787161/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                    ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg           <  1:925702/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg           <  1:2059278/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tCAATCATGGTTAAGGATGAACCGCTTACCGTCCCGTCCTCATCCACACAAAGTCCGTTACGGTAGTCTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGAACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGt          <  2:423943/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg           <  1:992513/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGa  >  1:1525497/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                     
TTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGAACTACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGA  >  CP042583/573979‑574536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A