Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 592,632 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583592,6320GC99.7% 699.5 / NA 294intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (142/151);  minor base A (1/0);  total (143/151)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP042583592,6330CG100.0% 693.1 / NA 294intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (143/151);  total (143/151)
*CP042583592,6340TA99.3% 692.0 / NA 294intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (140/151);  total (143/151)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 294 total aligned reads displayed.

GCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGGCTTTCGCAATTCTCAAAGACACCGCTAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGGCTTAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTACTCATTTTTCATCTCTTTATTTTAGG  >  CP042583/592352‑592913
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                       
  tgggCAAAGGATGGCGGGCGTGGGTGAATCAGGAGAGAGCCCATCGATAGGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCGCAAACTGATTCCCCGCCGGATGGCGACGGAGAAATTCGCCGCCCTGATGACGAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCCACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTGCTAACTGCCGACCATAAAATTCTCGTCGCGATTCGCaa                                                                                                                                                                                                                                                                                  <  2:1301717/285‑1 (MQ=255)
    tgCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAAtt                                                                                                                                                                                                                                                                                >  2:223411/1‑288 (MQ=255)
    tgCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGAATCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCaag                                                                                                                                                                                                                                                                                 >  1:915398/1‑286 (MQ=255)
      cAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATtctc                                                                                                                                                                                                                                                                             >  1:239514/1‑289 (MQ=255)
         tGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCGGCGGTTTCTAACTGGCGACCATAAAATTCTACTCGCGTTTCGCAATTTACaaa                                                                                                                                                                                                                                                                          >  2:1962064/1‑289 (MQ=255)
         tGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCaaa                                                                                                                                                                                                                                                                          >  2:1482435/1‑289 (MQ=255)
         tGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCaaa                                                                                                                                                                                                                                                                          >  1:777673/1‑289 (MQ=255)
         tGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGAAATTCTCaaa                                                                                                                                                                                                                                                                          >  2:1082508/1‑289 (MQ=255)
              ggCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGaca                                                                                                                                                                                                                                                                      >  1:929110/1‑288 (MQ=255)
                cgcgcCTTCGTGAATCAGCAGAACGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCCGGCAGCGGGTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACAccc                                                                                                                                                                                                                                                                   >  1:1140548/1‑288 (MQ=255)
                       tCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACAcccgaaatac                                                                                                                                                                                                                                                            <  1:330896/289‑9 (MQ=255)
                       tCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACAcccgaaatac                                                                                                                                                                                                                                                            <  2:177640/289‑9 (MQ=255)
                       tCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACAcccgaaata                                                                                                                                                                                                                                                             >  2:875643/1‑281 (MQ=255)
                             aTCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCa                                                                                                                                                                                                                                                      <  2:145939/289‑1 (MQ=255)
                                  cagATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAAt                                                                                                                                                                                                                                                   >  1:2148116/1‑287 (MQ=255)
                                      tCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATActc                                                                                                                                                                                                                                               <  2:347229/287‑1 (MQ=255)
                                      tCGCCCAGAGCTAGATCGGCGTCGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATActct                                                                                                                                                                                                                                              <  2:181229/288‑1 (MQ=255)
                                             tCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTac                                                                                                                                                                                                                                      <  1:875643/289‑1 (MQ=255)
                                              cGCTCCGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTaca                                                                                                                                                                                                                                     <  1:1372415/289‑1 (MQ=255)
                                                 tACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGGTTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACAAGt                                                                                                                                                                                                                                  >  1:1677645/1‑289 (MQ=255)
                                                   cGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGt                                                                                                                                                                                                                                  >  1:1774608/1‑287 (MQ=255)
                                                    gTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCt                                                                                                                                                                                                                               >  2:186453/1‑289 (MQ=255)
                                                        gCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAGTTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACCCGTTCTTGAt                                                                                                                                                                                                                           >  2:853111/1‑289 (MQ=255)
                                                        gCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGa                                                                                                                                                                                                                                                                        >  1:1239546/1‑244 (MQ=255)
                                                        gCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATActc                                                                                                                                                                                                                                               <  1:531166/269‑1 (MQ=255)
                                                        gCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATActc                                                                                                                                                                                                                                               >  2:531166/1‑269 (MQ=255)
                                                        gCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGAt                                                                                                                                                                                                                           >  1:428931/1‑289 (MQ=255)
                                                             gCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCCCAAAGACACCCGAAATACGGGGCACATACTCTGCGGGTACACCTTATTGATTGTCa                                                                                                                                                                                                                      >  2:1273176/1‑289 (MQ=255)
                                                               acaAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                                                                                                                                                     <  2:103845/288‑1 (MQ=255)
                                                               acaAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAg                                                                                                                                                                                                                     <  1:1485630/288‑1 (MQ=255)
                                                                caAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTa                                                                                                                                                                                                                   <  2:239514/289‑1 (MQ=255)
                                                                  aGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGc                                                                                                                                                                                                                 <  2:929110/289‑1 (MQ=255)
                                                                     cGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCaat                                                                                                                                                                                                              <  1:1082508/289‑1 (MQ=255)
                                                                               cAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCGATAAATACCggg                                                                                                                                                                                                    <  1:1334471/289‑1 (MQ=255)
                                                                                     cTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCa                                                                                                                                                                                              <  1:263831/289‑1 (MQ=255)
                                                                                     cTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCa                                                                                                                                                                                              <  2:1179423/289‑1 (MQ=255)
                                                                                       cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATa                                                                                                                                                                                            >  2:977816/1‑289 (MQ=255)
                                                                                       cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATa                                                                                                                                                                                            >  1:1278039/1‑289 (MQ=255)
                                                                                       cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATa                                                                                                                                                                                            <  2:1759184/289‑1 (MQ=255)
                                                                                       cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATa                                                                                                                                                                                            >  1:179488/1‑289 (MQ=255)
                                                                                       cACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTAAACGTTCTTGATTTTCAGt                                                                                                                                                                                                                    >  2:417397/1‑265 (MQ=255)
                                                                                               tATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTg                                                                                                                                                                                     >  1:689913/1‑288 (MQ=255)
                                                                                                  tCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACAcccgaaatac                                                                                                                                                                                                                                                            >  1:729891/1‑206 (MQ=255)
                                                                                                  tCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCa                                                                                                                                                                                  >  1:1741218/1‑288 (MQ=255)
                                                                                                              ttCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACACGAAATACGGGGCAAATACTCTGCGAGTACACGTTCTTGATTTGCAGTAGCAATAAATATCGGGATGCCATAACACGTGGCATCGATTTCATCGa                                                                                                                                                                     >  2:579213/1‑289 (MQ=255)
                                                                                                                   cgccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                                                                                                                                                                >  2:1848133/1‑241 (MQ=255)
                                                                                                                   cgccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCa                                                                                                                                                                                                                <  1:1848133/241‑1 (MQ=255)
                                                                                                                    gccgGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGtttt                                                                                                                                                                >  1:439330/1‑288 (MQ=255)
                                                                                                                         aTGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGCCCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCa                                                                                                                                                           <  2:1187801/288‑1 (MQ=255)
                                                                                                                         aTGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCa                                                                                                                                                           <  2:1732618/288‑1 (MQ=255)
                                                                                                                             cGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTaa                                                                                                                                                      >  2:2106002/1‑289 (MQ=255)
                                                                                                                              gACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTaa                                                                                                                                                      <  2:1718815/288‑1 (MQ=255)
                                                                                                                               aCGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACa                                                                                                                                                    >  2:483135/1‑289 (MQ=255)
                                                                                                                                                         cAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTgccgc                                                                                                                          <  1:483135/289‑1 (MQ=255)
                                                                                                                                                         cAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTgccg                                                                                                                           >  2:1913500/1‑288 (MQ=255)
                                                                                                                                                            ggCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTcc                                                                                                                                                              <  2:1656888/250‑1 (MQ=255)
                                                                                                                                                            ggCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTTAGCGATAAAACTATTGCCGCcac                                                                                                                       >  2:1671482/1‑289 (MQ=255)
                                                                                                                                                               aaTCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACAt                                                                                                                     <  1:2106002/288‑1 (MQ=255)
                                                                                                                                                               aaTCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACAt                                                                                                                     <  1:186453/288‑1 (MQ=255)
                                                                                                                                                                agcaacacggcTGTTACATTGATGGACATAATCGAACAGTGTGCGCGGAAGAAAGGTGGGCGGAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAAGTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATCCTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACAt                                                                                                                     <  2:2011834/280‑1 (MQ=255)
                                                                                                                                                                 tCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGAATCGATTTCATCGAGTTTTCCGCATTTAACATCGTTGAGCGATAAACCTATTTCCGCCACATCgt                                                                                                                  >  2:572492/1‑288 (MQ=255)
                                                                                                                                                                      cgcgCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATAtt                                                                                                              >  1:1688898/1‑288 (MQ=255)
                                                                                                                                                                           tGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACt                                                                                                        <  1:2085455/289‑1 (MQ=255)
                                                                                                                                                                               aCCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCa                                                                                                    <  2:645532/289‑1 (MQ=255)
                                                                                                                                                                                 cTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAACTTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCAtt                                                                                                  <  2:758303/289‑1 (MQ=255)
                                                                                                                                                                                     attgaCATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATg                                                                                              >  2:297726/1‑289 (MQ=255)
                                                                                                                                                                                      ttgaCATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATg                                                                                              <  1:1568623/288‑1 (MQ=255)
                                                                                                                                                                                       tgaCATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTa                                                                                            >  2:1685348/1‑289 (MQ=255)
                                                                                                                                                                                       tgaCATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTa                                                                                            >  1:2191054/1‑289 (MQ=255)
                                                                                                                                                                                          cttaatcagcga‑‑GTGCGCGGAAGAAAGGTGGGCGGCGTTGATTTTCATGATGGCTGGCAACGGTTTCTAACTGGATACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGTGGCAAATACTCTGCGGGTACACGTTCTTGATTTGCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGGGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGa                                                                                          <  2:1347083/277‑1 (MQ=255)
                                                                                                                                                                                           aTAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATa                                                                                        <  1:1671482/289‑1 (MQ=255)
                                                                                                                                                                                             aaTCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGATTTCCGCATGTAACATCGTTGAGCGATAAAACTATTTCCGCCACATCGATATTACGAATTTCATTAATGTAGATACa                                                                                      >  2:1402111/1‑289 (MQ=255)
                                                                                                                                                                                                   ccA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCAtt                                                                                                  <  2:2176374/271‑1 (MQ=255)
                                                                                                                                                                                                   ccA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATtctc                                                                                 >  1:837195/1‑288 (MQ=255)
                                                                                                                                                                                                    cA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTTCCGCCACACCTATATTACGACTTTCATTAATGTAGATACATTCTCg                                                                                >  2:1536225/1‑288 (MQ=255)
                                                                                                                                                                                                     a‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTa                                                                                                 >  1:1556585/1‑270 (MQ=255)
                                                                                                                                                                                                         tGCGCGGAAGAAAGGTGGGCGCATTTGATATTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGTCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTg                                                                             <  2:1551311/288‑1 (MQ=255)
                                                                                                                                                                                                         tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGtttt                                                                                                                                                                >  1:2241087/1‑205 (MQ=255)
                                                                                                                                                                                                         tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGc                                                                            >  1:139020/1‑289 (MQ=255)
                                                                                                                                                                                                         tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGc                                                                            >  1:67976/1‑289 (MQ=255)
                                                                                                                                                                                                         tGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTCCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCa                                                                                                    <  2:683732/265‑1 (MQ=255)
                                                                                                                                                                                                          gcgcGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTg                                                                             <  1:1183635/287‑1 (MQ=255)
                                                                                                                                                                                                          gcgcGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTg                                                                             <  1:564956/287‑1 (MQ=255)
                                                                                                                                                                                                           cgcgGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCg                                                                           <  1:1191081/288‑1 (MQ=255)
                                                                                                                                                                                                                   aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAAc                                                                  >  1:1454040/1‑289 (MQ=255)
                                                                                                                                                                                                                   aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAAc                                                                  >  1:1982693/1‑289 (MQ=255)
                                                                                                                                                                                                                   aaaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAAc                                                                  >  2:622342/1‑289 (MQ=255)
                                                                                                                                                                                                                    aaGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTaaaa                                                                   <  1:376989/287‑1 (MQ=255)
                                                                                                                                                                                                                         gggCGCGGTTGAGTTCCATAATGGCTGGCAGCGGTTTCTAACAGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACACGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTcc                                                             <  2:729868/288‑1 (MQ=255)
                                                                                                                                                                                                                          ggCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCCTCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGAAACCTTCTAGCTACTTGGTAAAACATGCCgg                                                           >  2:1222349/1‑289 (MQ=255)
                                                                                                                                                                                                                                      tttCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGAc                                                                                                         >  1:1655261/1‑231 (MQ=255)
                                                                                                                                                                                                                                                gctggcAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCACTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAAtt                                     >  1:1027931/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          gTTTCTAACTGGCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAAttttg                           >  1:1782791/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                     gCGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCa                 >  1:1612095/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                      cGACCATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCAt                <  2:1580071/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ccATAAAATTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATctct            <  1:1536225/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aaaTTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAtt       <  2:439330/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aaTTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAttt      <  1:40439/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aaTTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAttt      <  1:622342/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               aaTTCTCGTCGCGATTCGCAATTCTCAAAGACACCCGAAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGCGATAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTTGACATTTTTCATCTCTTTAttt      <  1:1954566/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                       
GCTGTGCAATGCATGGCGCGCCTTCGTGAATCAGCAGATCGCCCATCGCTACGTCGGCGTTGCACAAGTCGGCACGGAACAGCGCCTCACCAAAGTATTCCACAAACTGATTCCCCGCCGGATGGCGACGGAAAAATTCGCCGCCCTGATGACCAGGGCAATCAAACGCGCTGTTACCTTGATTGACATAATCGACCA‑‑GTGCGCGGAAGAAAGGTGGGCGCAGTTGAGTTTCATAATGGCTGGCAGCGGTTTCTAACTGGCGACCATAAAATTCTCGTCGGCTTTCGCAATTCTCAAAGACACCGCTAATACGGGGCAAATACTCTGCGGGTACACGTTCTTGATTTTCAGTAGCAATAAATACCGGGATGCCATAACCCGTGGCATCGATTTCATCGAGTTTTCCGCATGTAACATCGTTGAGGCTTAAAACTATTGCCGCCACATCGATATTACGACTTTCATTAATGTAGATACATTCTCGCTGCGTGGTAAAACAGTCCGGGCAAGAATCACTAACCGCAATTTTTAATTTACTCATTTTTCATCTCTTTATTTTAGG  >  CP042583/592352‑592913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A