Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 607,926 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583607,9260AT99.5% 496.6 / NA 211intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (117/92);  total (118/93)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01
*CP042583607,9270GC99.5% 495.4 / NA 211intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (117/93);  minor base A (1/0);  total (118/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP042583607,9280TA99.5% 497.0 / NA 211intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (117/93);  minor base C (1/0);  total (118/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

ATGCATTCTGGCGTAGCCCCTGGCAGCTTAGCAGCCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTAGTCTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCG  >  CP042583/607648‑608206
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
aTGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACGGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGaa                                                                                                                                                                                                                                                                                  <  1:394098/287‑1 (MQ=255)
          gcgttcgcccTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTaaaa                                                                                                                                                                                                                                                                      >  1:320118/8‑289 (MQ=255)
             ttcgcccTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGc                                                                                                                                                                                                                                                                    >  2:895734/5‑288 (MQ=255)
               cgcccTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACg                                                                                                                                                                                                                                                                 >  2:1767454/3‑289 (MQ=255)
                  ccTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTGAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGc                                                                                                                                                                                                                                                               <  1:1309480/288‑1 (MQ=255)
                         tcttagctcgcAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAg                                                                                                                                                                                                                                                       <  1:2036197/279‑1 (MQ=255)
                               ctcgcAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGt                                                                                                                                                                                                                                                 >  1:2192294/5‑289 (MQ=255)
                                tcgcAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCCCTAAAAGCACGGCAAGATCAGCAACGtt                                                                                                                                                                                                                                                >  2:2189599/4‑289 (MQ=255)
                                         aaCCGCATGGGCTATCGCTTACAGGGGCACATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGTTTCCACATAACGGGCAGCCGATTGTGTGTATGAACGACGCACAGACCACCGGTGATTCCCCCCGTATTGCCTGTATCATTGTGGCGGATATGTACCATCTGGCGCAAATTCCGCCCCGTCACCCGATTAATTTTGTCCAGGTTTCACTGGAATAGGCACTAAAAGCCCGGCAAGATCACCAActgtcatcttaac                                                                                                                                                                                                                                       >  2:1886512/1‑277 (MQ=255)
                                              cATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTa                                                                                                                                                                                                                                  >  1:637119/1‑289 (MQ=255)
                                                tGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCCACGAGCGCGAACAGTTATCTCACGGTTTGTTAACGGGCGTGGTGAAGGTGCCACATAACGGGCCGCCGATTGTGTTGATGAATGACGCACAGACCACCGGTGGTTACCCGCGTATAGACTTTATCATTGACGCCGATCTCTACCACCTGGCGCAAATTCACCTCTGTCAGCCGCACGAACTTGTCCAGTGTGCACTGGAATAGGCACTCAAACCACGCGAAGACACGAAACGTCATTTGGAATAATCAGc                                                                                                                                                                                                                                >  2:648871/1‑289 (MQ=255)
                                                tGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGGATCCTTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTTGTCACTGGAAAAGGCACTCACACGAATGCAAGATACGCAGCGTTAATTTTAACAAATAg                                                                                                                                                                                                                                 >  2:722673/1‑288 (MQ=255)
                                                       tCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTggcgg                                                                                                                                                                                                                         <  1:1846821/289‑1 (MQ=255)
                                                           ttACAGGGGCTAAGTTTGAAACGCACGACCGGTCGCGAACTGTAATCTCACGGTTTTGTACCGGGCGTTGTGCAGGTGCCACATAACGGGCAGCCGTTTGTGTTGATGAACCACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTg                                                                                                                                                                                                                      <  2:1995809/288‑1 (MQ=255)
                                                           ttACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                                                                                                                                     <  2:831746/289‑1 (MQ=255)
                                                            tACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                                                                                                                                     >  1:675419/1‑288 (MQ=255)
                                                             aCAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGCGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAAGTAGCGTGGCGGCTGcac                                                                                                                                                                                                                   >  1:587897/1‑289 (MQ=255)
                                                             aCAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGcac                                                                                                                                                                                                                   >  2:2069083/1‑289 (MQ=255)
                                                                 gggCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGGGGTGCAAGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAAt                                                                                                                                                                                                                <  2:482857/288‑1 (MQ=255)
                                                                  ggCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAAt                                                                                                                                                                                                                <  1:1067156/287‑1 (MQ=255)
                                                                     aaaTTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGGGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGaaaa                                                                                                                                                                                                           >  1:1921630/1‑289 (MQ=255)
                                                                          ttAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc                                                                                                                                                                                                      <  1:1979933/289‑1 (MQ=255)
                                                                            aaaaCGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCt                                                                                                                                                                                                    <  1:215201/289‑1 (MQ=255)
                                                                                       ccGGTCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATc                                                                                                                                                                                         <  2:320118/289‑1 (MQ=255)
                                                                                          aTCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGc                                                                                                                                                                                                                                                                    >  2:223398/1‑211 (MQ=255)
                                                                                             gcgAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCg                                                                                                                                                                                   <  2:1040889/289‑1 (MQ=255)
                                                                                                     ttATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAAAATTTAGCGTGGCGGAGGCACCATGAAAATTGACCTGAACGCCGATCTGGGCGActgctg                                                                                                                                                                            >  2:1420656/1‑288 (MQ=255)
                                                                                                      tATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGGGGCGGCTGTACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAggcggc                                                                                                                                                                           >  1:490645/1‑288 (MQ=255)
                                                                                                          tcACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTACACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAg                                                                                                                                                                      <  1:1814472/289‑1 (MQ=255)
                                                                                                             cGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAACATCAGCAACGTTATTTTGAACAATTACCGTGGCGTCTGCCCAATGAAAATTCACCTGAACGCCGAt                                                                                                                                                                                          >  2:2193793/1‑266 (MQ=255)
                                                                                                              ggTTTGTTCCCGGGCGGGGCGCAGGGTCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGAc                                                                                                                                                                  <  2:560160/289‑1 (MQ=255)
                                                                                                                 ttGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCa                                                                                                                                                               <  1:895734/289‑1 (MQ=255)
                                                                                                                   gTTCCCTGGCGGGGTGGAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGACCGACGCACAGACCACCGGTGGTTACCCGCGGATGGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCAGTTTGTCAAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCaga                                                                                                                                                             <  2:1916055/289‑1 (MQ=255)
                                                                                                                      aCCGGGCGTGGTGCAGGCGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAAtt                                                                                                                                                                                                         >  2:872627/1‑242 (MQ=255)
                                                                                                                          ggCGTGGTGCAGGTGCCTCATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCATTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATc                                                                                                                                                                                         >  2:246669/1‑254 (MQ=255)
                                                                                                                          ggCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGAGCCAGCGACGCAGAGCTATTa                                                                                                                                                      >  1:1080329/1‑289 (MQ=255)
                                                                                                                              tggtgCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAAc                                                                                                                                                    >  1:229454/1‑287 (MQ=255)
                                                                                                                              tggtgCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAAc                                                                                                                                                    >  1:1204243/1‑287 (MQ=255)
                                                                                                                                  gCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGt                                                                                                                                              <  1:561204/289‑1 (MQ=255)
                                                                                                                                     ggTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGttt                                                                                                                                            >  2:1542757/1‑288 (MQ=255)
                                                                                                                                      gTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGttt                                                                                                                                            >  1:211457/1‑287 (MQ=255)
                                                                                                                                           acaTAACGGGCATCCTATTGTGTTTATTAACGACGGACAGTCCACCTGTGGTTTCCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCTAATTCCGCTCGGTCAGCCGTTTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGc                                                                                                                                     <  1:1420656/289‑1 (MQ=255)
                                                                                                                                           acaTAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTg                                                                                                                                      >  2:672599/1‑288 (MQ=255)
                                                                                                                                           acaTAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTg                                                                                                                                      >  1:1242428/1‑288 (MQ=255)
                                                                                                                                                 cGGGCAGCCGGTTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAatat                                                                                                                               <  2:454971/289‑1 (MQ=255)
                                                                                                                                                 cGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAatat                                                                                                                               >  2:1799144/1‑289 (MQ=255)
                                                                                                                                                     cAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGc                                                                                                                            <  2:1127152/288‑1 (MQ=255)
                                                                                                                                                        ccGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATCAACGCCGGTTTCCTTCGCCAATATTGCCttt                                                                                                                        >  2:605513/1‑287 (MQ=255)
                                                                                                                                                         cGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCt                                                                                                                          <  1:268356/286‑1 (MQ=255)
                                                                                                                                                             ataagagcccgacgacgCACAGACCACCGGTGGTTACCCTCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGAt                                                                                                                    <  1:1201715/277‑1 (MQ=255)
                                                                                                                                                                   gaggaACAACGCCCAGACCCACGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGCTATGTACCATCGGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGc                                                                                                             <  2:2039638/286‑1 (MQ=255)
                                                                                                                                                                    atgaACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAat                                                                                                                                 >  2:215193/1‑268 (MQ=255)
                                                                                                                                                                        acgcagccccgACCCCCGGTGGTTACCCGCGTATTGCCTGTTTCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCg                                                                                                        <  2:589621/279‑1 (MQ=255)
                                                                                                                                                                        acgacgCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCg                                                                                                        <  1:1586762/289‑1 (MQ=255)
                                                                                                                                                                         cgacgCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTAt                                                                                                                                                        >  2:916353/1‑240 (MQ=255)
                                                                                                                                                                              cacaGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATgcgc                                                                                                  >  1:1273218/1‑289 (MQ=255)
                                                                                                                                                                               accgACCCCCGTGGGTTACCGGCTTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGCACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAATGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCa                                                                                                 <  2:1301944/286‑1 (MQ=255)
                                                                                                                                                                               acaGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCa                                                                                                 <  2:398897/289‑1 (MQ=255)
                                                                                                                                                                                   accaccGGTGGTTACTCGCGTATTGCCTGTAACATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGTCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTAATAACGCTGGTTTCCTCGGCCAATATTGCCTGTCATTTTCATTCAGGACATGGCCAAATc                                                                                             >  2:1818102/1‑289 (MQ=255)
                                                                                                                                                                                    ccaccGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCaaa                                                                                               <  1:1614677/286‑1 (MQ=255)
                                                                                                                                                                                    caaccGGGGGTTACCGGCGTATTGCCTGTATCATTGAGGCTGATTTGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCaaa                                                                                               <  2:2090770/284‑1 (MQ=255)
                                                                                                                                                                                       ccGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGc                                                                                         <  1:1960567/289‑1 (MQ=255)
                                                                                                                                                                                         ggtgttTACCCGCGTGTTGCATGTATCATTGAGGCTGATATGTCCCATCTGGCGCAAATTCCGCTCGGTCCGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAg                                                                                       <  1:1035788/289‑1 (MQ=255)
                                                                                                                                                                                             gtTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAg                                                                                                                                                                      <  2:114620/206‑1 (MQ=255)
                                                                                                                                                                                             gtTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAg                                                                                                                                                                      >  1:114620/1‑206 (MQ=255)
                                                                                                                                                                                             gtTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCaga                                                                                                                                                             >  1:155640/1‑215 (MQ=255)
                                                                                                                                                                                             gtTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCaaa                                                                                               >  1:606123/1‑277 (MQ=255)
                                                                                                                                                                                             gtTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCtt                                                                                   >  1:1650382/1‑289 (MQ=255)
                                                                                                                                                                                               tACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAagag                                                                                                                                                                                                                                                                               <  2:2256836/99‑1 (MQ=255)
                                                                                                                                                                                                         ttGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACATTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAg                                                                       <  2:286375/289‑1 (MQ=255)
                                                                                                                                                                                                         ttGCCGGTACAATTGAGTGTGGTATTGACCCTGTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAg                                                                       <  2:1831942/289‑1 (MQ=255)
                                                                                                                                                                                                           gCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCa                                                                     <  2:1650382/289‑1 (MQ=255)
                                                                                                                                                                                                                tATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACATTTAGCGTGGCGGCTGCCCAATGAAAATTGACCTGAACGCCGTTCTGGGCGAAGGCTGCGCCAGCGACGCAGGGCTATTAACCCTGGTTTCCTCGCCCAATATTGCCTGTGGCTTTCCGGCAGGCGATCGCCAAACCATGCAGGTTTGCGTGGGTGTAGCaaaaaa                                                                >  2:2176567/1‑289 (MQ=255)
                                                                                                                                                                                                                tATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATaaa                                                                >  2:1085514/1‑289 (MQ=255)
                                                                                                                                                                                                                  tCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATaaaaa                                                              <  2:159389/289‑1 (MQ=255)
                                                                                                                                                                                                                        aGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAACATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATCTTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCCGGAAGCAACAAAAAAAGgtt                                                        >  2:1965923/1‑288 (MQ=255)
                                                                                                                                                                                                                          gCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGcc                                                                                                                                    <  2:1221736/211‑1 (MQ=255)
                                                                                                                                                                                                                                    aCCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGgcg                                            <  1:2053484/289‑1 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                                                                                                                                     <  2:939450/116‑1 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                                                                                                                                     >  1:939450/1‑116 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTTCGTGAAGCAATAAAAAATGGTGTCGCGATTGccgccc                                         >  2:1477991/1‑286 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTc                                         >  1:2074826/1‑288 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTc                                         >  1:536374/1‑288 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCa                                        >  1:1521162/1‑289 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATAGGAGCTc                                         >  1:1769580/1‑288 (MQ=255)
                                                                                                                                                                                                                                        tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAACTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCCTGCGTGCGTGCAGCAATAAAAAATGGTGTCGCGATTGGCGCTc                                         >  1:1791029/1‑288 (MQ=255)
                                                                                                                                                                                                                                           ggCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCAccc                                     >  2:945324/1‑289 (MQ=255)
                                                                                                                                                                                                                                           ggCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCAccc                                     >  1:1640678/1‑289 (MQ=255)
                                                                                                                                                                                                                                             cgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGgcg                                            >  1:1724147/1‑280 (MQ=255)
                                                                                                                                                                                                                                             cgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGgcg                                            <  2:1724147/280‑1 (MQ=255)
                                                                                                                                                                                                                                             cgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGa                                   >  1:240521/1‑289 (MQ=255)
                                                                                                                                                                                                                                             cgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGa                                   >  1:1134957/1‑289 (MQ=255)
                                                                                                                                                                                                                                              gcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGa                                   <  1:798949/288‑1 (MQ=255)
                                                                                                                                                                                                                                              gcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGa                                   <  1:2239424/288‑1 (MQ=255)
                                                                                                                                                                                                                                              gcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGa                                   <  1:2189599/288‑1 (MQ=255)
                                                                                                                                                                                                                                                             taaGCCGATTAATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGGGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAggg                    <  2:1046255/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ttCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTgg                                              <  1:404248/254‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ttttGTCCAGGGTTCACTGGAAGAGGAACTAAAAGAACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCg       <  2:1466779/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ttGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCa     <  2:1242428/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ttGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCa     <  1:1496726/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
ATGCATTCTGGCGTAGCCCCTGGCAGCTTAGCAGCCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTAGTCTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCG  >  CP042583/607648‑608206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A