Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 630,887 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583630,8870AT99.6% 640.0 / NA 271intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (138/130);  total (141/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583630,8880GC99.3% 633.9 / NA 271intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (141/127);  minor base A (0/2);  total (141/130)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583630,8890TA99.6% 649.6 / NA 271intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (141/129);  minor base G (0/1);  total (141/130)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

CCTTAGCACACCCGGTGCCGGCATTTTCAGCCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCAGCACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGAGTCGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGAT  >  CP042583/630602‑631167
                                                                                                                                                                                                                                                                                             |||                                                                                                                                                                                                                                                                                       
cctttcgacacCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTAACTGACTGATGGCGTAATGCCATCAACAAAAAGGAAACGACGATGAAAGCAGTAACTCGCGTTACCAACACCCCGAAAAAGAACTGGGCTCGTGCATGGTATTTCAACCCCGAagagacc                                                                                                                                                                                                                                                                                         >  2:1836939/8‑286 (MQ=255)
        cacCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑Tctg                                                                                                                                                                                                                                                                               <  1:149549/289‑1 (MQ=255)
           ccGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGTGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑Tctgctg                                                                                                                                                                                                                                                                            >  1:127441/1‑289 (MQ=255)
           ccGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑Tctgctg                                                                                                                                                                                                                                                                            <  2:153187/289‑1 (MQ=255)
           ccGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑Tctgctg                                                                                                                                                                                                                                                                            <  1:990384/289‑1 (MQ=255)
              gTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTc                                                                                                                                                                                                                                                                         >  2:760611/1‑289 (MQ=255)
              gTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGGTTGGCTCGGCATGGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCACCTATCTGACTGACGGCTTAAGGCCATAAAATAAAAGGAAACGACGATGAAAGACGTAACGCCCGGTCACAATACCCCGCAACTTCACTAGAAACAAAAGTGGTATTTGACCTCCGAAGACTCACGTAA‑TCTGCTGGt                                                                                                                                                                                                                                                                          >  2:2173109/1‑288 (MQ=255)
                gCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCaa                                                                                                                                                                                                                                                                       <  1:1364291/289‑1 (MQ=255)
                 ccGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCaa                                                                                                                                                                                                                                                                       <  2:1398311/288‑1 (MQ=255)
                    gCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGATGGGTATTTCACCCCCGAAGAGACACGTAT‑TCTACTGGCCatgaaa                                                                                                                                                                                                                                                                   >  2:1207713/1‑284 (MQ=255)
                      attttctcgctctTTTTACTTCATGCTAAGGGCGCTGGCGGTGTTATTGCGTCAATTGGGTGGTTTGGCGCGGCATTGGTGGTGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATTAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCGCGTAT‑TCTGCTGGTCAataata                                                                                                                                                                                                                                                                  <  1:21080/279‑1 (MQ=255)
                                          tactgttgatgggtcgggcggTGTTATGGCTGCAATTGTCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGAAGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGAc                                                                                                                                                                                                                                             <  2:539769/275‑1 (MQ=255)
                                              gATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGTTGGTTTGGCGCTGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCAAGGCAACTATCTGAATGATGGCCTAATGCCATAAACAAAAAGGAAATGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACTACCGAAGCGTCACCTAC‑TTTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGa                                                                                                                                                                                                                                         >  1:1933370/1‑289 (MQ=255)
                                                tAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGaa                                                                                                                                                                                                                                        <  1:1395907/288‑1 (MQ=255)
                                                  aTGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGAc                                                                                                                                                                                                                                             >  1:1441998/1‑281 (MQ=255)
                                                      cgcgGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTTAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACCAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTCGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACa                                                                                                                                                                                                                                 >  1:1338175/1‑289 (MQ=255)
                                                      cgcgGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACa                                                                                                                                                                                                                                 >  1:981936/1‑289 (MQ=255)
                                                      cgcgGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACa                                                                                                                                                                                                                                 >  1:2255346/1‑289 (MQ=255)
                                                       gcgggtggGGGTATGGCGGCAATTGGCTGGTTGGGGGCGGCATTGGTGGGGGCTGCAATCTCGACTGAAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACa                                                                                                                                                                                                                                 <  2:1499364/288‑1 (MQ=255)
                                                          ggcggTGTTATGGCGGGAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACg                                                                                                                                                                                                                              <  1:1021959/288‑1 (MQ=255)
                                                          ggcggTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGCTCCTGTGCCGACTGGACCAGGACCACgc                                                                                                                                                                                                                             >  2:1021959/1‑288 (MQ=255)
                                                               tgtTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATAc                                                                                                                                                                                                                         <  1:382465/288‑1 (MQ=255)
                                                                     ggcggcAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCTAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGACATCCTGTTCCGAATGGCACAGGACAACGAATACAAATAtt                                                                                                                                                                                                                  >  2:1706905/1‑289 (MQ=255)
                                                                     ggcggcAATTGGCTGGTTTGGCACGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTCATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCCCGCGTTCACATGACCCAGCATAGGACCTGGGATCGAGAGCGTTATTTAACCCACAAAGAGTCAAGGAT‑TCTGCTGGTCACTCATATGGAATCACTCCCGTGCCGCCAGGCAAAGGAACACGAATACAAaaata                                                                                                                                                                                                                  >  2:1574845/1‑288 (MQ=255)
                                                                         gcAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGt                                                                                                                                                                                                              >  2:1331857/1‑289 (MQ=255)
                                                                         gcAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGt                                                                                                                                                                                                              >  2:818803/1‑289 (MQ=255)
                                                                         gcAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGt                                                                                                                                                                                                              >  2:1189838/1‑289 (MQ=255)
                                                                             ttGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAATCAGTATCTCGCGTTCACATCACCCCGCATATGCACTTGGATCGAGAGTGGTATGTCACCACCGAAGAGTCACGTATCTCT‑CTGGTCAATAAGTGGGAAGAGATCCTGTGCCGACTGGAACAGGACCAGGAAAACAAATATTACGTACTc                                                                                                                                                                                                          >  2:64259/1‑289 (MQ=255)
                                                                               ggCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGa                                                                                                                                                                                                        <  2:1995623/289‑1 (MQ=255)
                                                                               ggCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGa                                                                                                                                                                                                        <  1:1165850/289‑1 (MQ=255)
                                                                                gCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGAc                                                                                                                                                                                                                                             <  1:1675681/251‑1 (MQ=255)
                                                                                                  tggtggGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTc                                                                                                                                                                                     <  1:1501280/289‑1 (MQ=255)
                                                                                                         ggCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATAc                                                                                                                                                                                                                         >  1:579705/1‑246 (MQ=255)
                                                                                                         ggCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATAc                                                                                                                                                                                                                         <  2:579705/246‑1 (MQ=255)
                                                                                                         ggCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGatt                                                                                                                                                                              >  1:2170593/1‑289 (MQ=255)
                                                                                                              caatctcgaCTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACCACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGCTTATTTc                                                                                                                                                                         >  2:2035470/9‑289 (MQ=255)
                                                                                                              caatctcgaCTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTc                                                                                                                                                                         >  1:1857491/9‑289 (MQ=255)
                                                                                                                                      tgtgGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGa                                                                                                                                                  <  2:1066877/288‑1 (MQ=255)
                                                                                                                                      tgtgGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGAc                                                                                                                                                 <  2:560749/289‑1 (MQ=255)
                                                                                                                                       gttgaggggTCAAGCGGTTATGCATTGCAAATATCTGCCTTATGGCGGTATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGATTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGAc                                                                                                                                                 <  2:2242526/280‑1 (MQ=255)
                                                                                                                                         ggcggcggcACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAAAAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCg                                                                                                                                               <  1:1705605/288‑1 (MQ=255)
                                                                                                                                          gcggcgTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                                                                                                            >  1:1525914/1‑274 (MQ=255)
                                                                                                                                           cggcggCACGCTGTTAAGCATGGCAACTATCTGACTGATGGCGTAGTGCCATAAACAAACAGGAAGAGACGATGAAAGCAGTATCTCGCCGTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATATCGCCACCGAAGAGTCACGTAT‑TCTGCTGGTTAATAATATGGAAGCGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCgtg                                                                                                                                             <  2:1879335/288‑1 (MQ=255)
                                                                                                                                               gTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGTAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTgaag                                                                                                                                        >  1:658083/1‑289 (MQ=255)
                                                                                                                                                          aaGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGa                                                                                                                             >  1:237148/1‑289 (MQ=255)
                                                                                                                                                                  cAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCaataat                                                                                                                                                                                                                                                                   >  1:1757139/1‑147 (MQ=255)
                                                                                                                                                                  cAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCa                                                                                                                     >  1:876729/1‑289 (MQ=255)
                                                                                                                                                                  cAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCa                                                                                                                     >  1:1479712/1‑289 (MQ=255)
                                                                                                                                                                            actgaTGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTa                                                                                                            >  1:1560184/1‑288 (MQ=255)
                                                                                                                                                                                aTGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCgg                                                                                                        >  1:837892/1‑288 (MQ=255)
                                                                                                                                                                                  ggagTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                                     <  2:1984420/286‑1 (MQ=255)
                                                                                                                                                                                  ggCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                                     <  2:1752669/289‑1 (MQ=255)
                                                                                                                                                                                       agtGCCATCAACAAAAAGGAAACGGCGATGAAAGCAGGATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGGGGTATGTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGAGGGGCAAGCTGCGAGCCTCGAAGATTATTTCGCGGGGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGt                                                                                                <  2:33712/287‑1 (MQ=255)
                                                                                                                                                                                             aTAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGGCCCTGGTATAcgc                                                                                          >  1:421134/1‑287 (MQ=255)
                                                                                                                                                                                              tAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGAc                                                                                                                                                 <  2:187098/233‑1 (MQ=255)
                                                                                                                                                                                              tAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAAAGGAGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCa                                                                                         >  1:1791682/1‑289 (MQ=255)
                                                                                                                                                                                                  cAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGAcc                                                                                     <  1:1873964/289‑1 (MQ=255)
                                                                                                                                                                                                    aaaaGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGa                                                                                   <  1:863755/289‑1 (MQ=255)
                                                                                                                                                                                                     aaaGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                                                                                                            >  1:687859/1‑215 (MQ=255)
                                                                                                                                                                                                     aaaGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                                                                                                            <  2:687859/215‑1 (MQ=255)
                                                                                                                                                                                                     aaaGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGGCGGCAAGATGATTAACGGACCCGGGGAAAcc                                                                                           >  2:1664487/1‑280 (MQ=255)
                                                                                                                                                                                                     aaaGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                                     >  2:839082/1‑270 (MQ=255)
                                                                                                                                                                                                      aaGGAAACGACGATGAAAGCAGTATCTCGCGTTCAAATCACCCCGCATTTGCACTGGGATCGAGAGTCGTATTTCACCACCGAAGAGTAACTTAT‑TCTGCTGGTCAATAATATTGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAAAACAAATATTACTTACTCGTCGGGCAAACGGCGATCCTCTAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACACGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATa                                                                                 <  1:1378450/289‑1 (MQ=255)
                                                                                                                                                                                                                      aaGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGTGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGCTCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGc                                                                 <  2:137473/289‑1 (MQ=255)
                                                                                                                                                                                                                               ctagcgGTCACATCACCCCGAATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAAAAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCAt                                                        <  2:1691748/286‑1 (MQ=255)
                                                                                                                                                                                                                                 cgcgTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCg                                                      >  2:1139494/1‑289 (MQ=255)
                                                                                                                                                                                                                                   cgTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATc                                                                                                          >  2:1546267/1‑235 (MQ=255)
                                                                                                                                                                                                                                    gTTCACATCACCCCGCATATGCACTGGGATCGAGAGGGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTcc                                                   <  1:1706905/289‑1 (MQ=255)
                                                                                                                                                                                                                                      tCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCTTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGt                                                 >  1:33224/1‑289 (MQ=255)
                                                                                                                                                                                                                                        acaTCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTaa                                               >  1:255778/1‑289 (MQ=255)
                                                                                                                                                                                                                                         caTCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACTTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGAGGGTCGAAGATTATTGCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGTCAAGTTGATTATCGGCCCCGGGTATACCCAGACCGGTACCACGATTGTGTCTGGGGAATCCATCGGCCGtggt                                              <  1:1629547/289‑4 (MQ=255)
                                                                                                                                                                                                                                           tCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCt                                            <  1:1033253/289‑1 (MQ=255)
                                                                                                                                                                                                                                            cACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTg                                           >  2:1125292/1‑289 (MQ=255)
                                                                                                                                                                                                                                              ccccGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACACCGAATACAAATATTACGTACTCGACGGGCAAACGGAGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCAGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGAGTGTTTCTGCGGAATCCATCGGCAGTATTCTGAt                                         >  2:414530/1‑289 (MQ=255)
                                                                                                                                                                                                                                              ccccGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTaa                                               >  1:2032219/1‑283 (MQ=255)
                                                                                                                                                                                                                                                 cGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTa                                      <  1:557600/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    atatGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCaata                                                                                                                                                                                                                                                                     >  1:285573/1‑63 (MQ=255)
                                                                                                                                                                                                                                                    atatGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCaata                                                                                                                                                                                                                                                                     <  2:285573/63‑1 (MQ=255)
                                                                                                                                                                                                                                                         cACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGc                              >  2:1504934/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            tGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTg                           >  1:524724/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            tGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTAGCGGCCCCTGGTATACCCAGACCGATACCACGATTTTTTCTGCGGAATCCAACGGCCGTAATCTGATGTACGGAATGCGTg                           >  2:936197/1‑289 (MQ=255)
                                                                                                                                                                                                                                                             gggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGt                                                                       <  1:2063503/244‑1 (MQ=255)
                                                                                                                                                                                                                                                             gggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTg                           >  2:2129051/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             gggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTg                           >  1:155309/1‑288 (MQ=255)
                                                                                                                                                                                                                                                              ggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                                                                                                            >  2:1361223/1‑158 (MQ=255)
                                                                                                                                                                                                                                                              ggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTgg                                                                                                 >  1:1197532/1‑217 (MQ=255)
                                                                                                                                                                                                                                                              ggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGt                                                                                                >  2:148469/1‑218 (MQ=255)
                                                                                                                                                                                                                                                              ggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTg                           >  1:753487/1‑287 (MQ=255)
                                                                                                                                                                                                                                                              ggATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTg                           >  1:1142609/1‑287 (MQ=255)
                                                                                                                                                                                                                                                              ggATCGAGAGTGGTATTTCACCACCGAAGAGCCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAACGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGAGTGTTTCTGCGGAATCCATCGTTCGTAATCTGATGTACGGAATGCgag                           >  2:800609/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                        tGGTATTTCACCACCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                                                                                                            >  2:1619128/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                                caccacCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAg       <  1:936197/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                caccacCGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGGGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAg       <  2:981936/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                                     <  2:673760/190‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATg                               <  1:1242676/260‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATg                               >  2:1242676/1‑260 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGAt  >  2:1764232/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGAt  >  1:1176492/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGAt  >  1:791223/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGAAGAGTCACGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTAATTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGAt  >  2:2258325/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                             |||                                                                                                                                                                                                                                                                                       
CCTTAGCACACCCGGTGCCGGCATTTTCAGCCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCAGCACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGAGTCGTAT‑TCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGAT  >  CP042583/630602‑631167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A