Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 781,967 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583781,9670GC99.7% 698.7 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (128/163);  total (128/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
*CP042583781,9680CG99.7% 692.0 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (128/164);  total (128/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583781,9690TA99.7% 700.4 / NA 293intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (128/163);  total (128/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TGCTTACGGCTAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAAGCTGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGACTCAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGGCT‑CATATTCATGAAATCAATGGTTATACATGGCGTGCTTTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTATCAGACGCTGATT  >  CP042583/781689‑782248
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                        
tGCTTACGGCTAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGCAGTTACCCGGCCGACACTGTCCACGTGCCAGACGAAGAAAAAGTGACAGGCTGCTGAGAGATTCACATGGCCAAAAGGTCCGTTCAGATAGCTgggcctaattaa                                                                                                                                                                                                                                                                                  >  2:1390637/1‑279 (MQ=255)
   ttACGGCTAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATg                                                                                                                                                                                                                                                                               <  1:125914/288‑1 (MQ=255)
        gCTAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATc                                                                                                                                                                                                                                                                          <  2:779087/288‑1 (MQ=255)
          tAAGGTTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGATTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCaa                                                                                                                                                                                                                                                                        <  1:1817586/288‑1 (MQ=255)
          tAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCaa                                                                                                                                                                                                                                                                        <  2:1577025/288‑1 (MQ=255)
          tAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCaa                                                                                                                                                                                                                                                                        <  1:1237884/288‑1 (MQ=255)
          tAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGAACAGGCAGTCCGGGCTGGCGCGTTAAAAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCaa                                                                                                                                                                                                                                                                        <  2:2238019/288‑1 (MQ=255)
                 cgccgcAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATg                                                                                                                                                                                                                                                                      <  2:142554/283‑1 (MQ=255)
                 cgccgcAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATg                                                                                                                                                                                                                                                                      >  1:142554/1‑283 (MQ=255)
                    cgcAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACa                                                                                                                                                                                                                                                              <  1:64512/288‑1 (MQ=255)
                    cgcAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACa                                                                                                                                                                                                                                                              <  1:1107526/288‑1 (MQ=255)
                            aaGGGCGATGCACGCTAACAGGGAGTAGCGGCTGTCGCGTTAACACTACATAGAGCGCAGGCCAGTGGGAAAGTTCGTTCATCCACTCAAGTCGTAAACGGTGCGTAATCAAAGAGCGCATCAAAAATTTCTGGCGAAAACTACCGCGATGCCAGAATTTACCCGGCCGACATAGACCACGTGCTAGAGTAAGATAACGTGACAGGCTTGTGAGATATTCAGATGGCGTAAAGGGCCGTTCAGTGAGC‑GGCTACATATTCATGAAATCCGTTGTTAGACATGGCGtcga                                                                                                                                                                                                                                                     <  2:742111/289‑4 (MQ=255)
                                gCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGtcgattt                                                                                                                                                                                                                                                  <  2:197159/288‑7 (MQ=255)
                                   aTGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAcc                                                                                                                                                                                                                                              >  2:1008956/1‑289 (MQ=255)
                                   aTGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAc                                                                                                                                                                                                                                               >  1:200659/1‑288 (MQ=255)
                                   aTGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAc                                                                                                                                                                                                                                               <  2:2052020/288‑1 (MQ=255)
                                   aTGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAc                                                                                                                                                                                                                                               >  2:1131903/1‑288 (MQ=255)
                                    tGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCaa                                                                                                                                                                                                                                                                        <  2:803486/262‑1 (MQ=255)
                                    tGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCaa                                                                                                                                                                                                                                                                        >  1:803486/1‑262 (MQ=255)
                                    tGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAcc                                                                                                                                                                                                                                              <  1:1566426/288‑1 (MQ=255)
                                     gCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCAt                                                                                                                                                                                                                                            >  2:1105640/1‑289 (MQ=255)
                                         aCGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCg                                                                                                                                                                                                                                        >  1:1697400/1‑289 (MQ=255)
                                              caGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAcc                                                                                                                                                                                                                                              >  1:413587/1‑278 (MQ=255)
                                                   aGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAAc                                                                                                                                                                                                                               <  1:1217865/288‑1 (MQ=255)
                                                   aGTCGCGGCTGGAGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAAc                                                                                                                                                                                                                               <  2:909470/288‑1 (MQ=255)
                                                     tCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCaa                                                                                                                                                                                                                            >  1:1550041/1‑289 (MQ=255)
                                                      cgcgGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCa                                                                                                                                                                                                                             <  1:1008956/287‑1 (MQ=255)
                                                             ggCGCGTTCACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCAGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAAt                                                                                                                                                                                                                    <  2:1280436/289‑1 (MQ=255)
                                                               cgcgTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAg                                                                                                                                                                                                                  >  1:532088/1‑289 (MQ=255)
                                                                            cATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAAc                                                                                                                                                                                                                               <  1:1973272/263‑1 (MQ=255)
                                                                            cATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAAc                                                                                                                                                                                                     >  1:915950/1‑289 (MQ=255)
                                                                                  gaTTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACAATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAAt                                                                                                                                                                                               >  1:979483/1‑289 (MQ=255)
                                                                                      aGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTa                                                                                                                                                                                           >  2:1507857/1‑289 (MQ=255)
                                                                                       ggCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAg                                                                                                                                                                                          >  1:244884/1‑289 (MQ=255)
                                                                                        gCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAgg                                                                                                                                                                                         >  1:832474/1‑289 (MQ=255)
                                                                                            gTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCAcc                                                                                                                                                                                                                                              >  1:972899/1‑232 (MQ=255)
                                                                                             tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAggg                                                                                                                                                                                        <  2:1545787/285‑1 (MQ=255)
                                                                                             tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGtttt                                                                                                                                                                                    >  1:1717341/1‑289 (MQ=255)
                                                                                             tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGtttt                                                                                                                                                                                    >  2:385088/1‑289 (MQ=255)
                                                                                                             tCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGa                                                                                                                                                                    >  1:2078987/1‑289 (MQ=255)
                                                                                                                  tCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTg                                                                                                                                                                                                                                          <  1:1141755/214‑1 (MQ=255)
                                                                                                                      cGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATCTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGata                                                                                                                                                                             >  1:61006/1‑271 (MQ=255)
                                                                                                                      cGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATCTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGata                                                                                                                                                                             <  2:61006/271‑1 (MQ=255)
                                                                                                                       gCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGAc                                                                                                                                                          <  2:590649/289‑1 (MQ=255)
                                                                                                                         ttAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGAcc                                                                                                                                                         <  2:200659/288‑1 (MQ=255)
                                                                                                                          tAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCg                                                                                                                                                       >  2:1145829/1‑289 (MQ=255)
                                                                                                                           caaacgggCCTAATCACCCAGCGCAGCAAAAATTTCTGGCGAAAACTCCGGCGATGACAGAAGTTA‑CCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGAAAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATTAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGa                                                                                                                                                     <  2:2083333/283‑1 (MQ=255)
                                                                                                                           aaaCGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCgg                                                                                                                                                      >  2:1845515/1‑289 (MQ=255)
                                                                                                                              cgcgGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGAtt                                                                                                                                                   <  1:321938/289‑1 (MQ=255)
                                                                                                                              cgcgGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGAtt                                                                                                                                                   <  2:976498/289‑1 (MQ=255)
                                                                                                                                cgGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTgg                                                                                                                                                 >  2:1732208/1‑289 (MQ=255)
                                                                                                                                  gCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTa                                                                                                                                                                                           <  1:291195/245‑1 (MQ=255)
                                                                                                                                      aatcaacgagcgcAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATg                                                                                                                                           >  1:5598/10‑289 (MQ=255)
                                                                                                                                                  cagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGaa                                                                                                                               >  1:180019/1‑289 (MQ=255)
                                                                                                                                                           tttCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGa                                                                                                                      <  2:244884/289‑1 (MQ=255)
                                                                                                                                                             tCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGaaa                                                                                                                    <  1:1003720/289‑1 (MQ=255)
                                                                                                                                                               tGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGAtt                                                                                                                                                   <  1:1446794/256‑1 (MQ=255)
                                                                                                                                                                               gATGACAGAATTTACCCGGCCGACACTGTCAACGGGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTgaag                                                                                                  <  2:28800/289‑1 (MQ=255)
                                                                                                                                                                                 tGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTgaagaa                                                                                                >  2:1627519/1‑289 (MQ=255)
                                                                                                                                                                                    cAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcg                                                                                             <  1:587000/289‑1 (MQ=255)
                                                                                                                                                                                         tttACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgttacg                                                                                        <  1:396970/289‑1 (MQ=255)
                                                                                                                                                                                             cccGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGc                                                                                                                                                >  2:1710736/1‑229 (MQ=255)
                                                                                                                                                                                              ccGGCCGAAACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTAtt                                                                                   <  1:1846126/289‑1 (MQ=255)
                                                                                                                                                                                                  ccGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcg                                                                                <  2:92835/288‑1 (MQ=255)
                                                                                                                                                                                                  ccGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcg                                                                                <  2:130088/288‑1 (MQ=255)
                                                                                                                                                                                                  ccGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcg                                                                                <  2:832474/288‑1 (MQ=255)
                                                                                                                                                                                                   cGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcgc                                                                               <  1:2144847/288‑1 (MQ=255)
                                                                                                                                                                                                       acTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgtta                                                                                          >  1:2230060/1‑273 (MQ=255)
                                                                                                                                                                                                        cTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGa                                                                         <  1:1627519/289‑1 (MQ=255)
                                                                                                                                                                                                           tCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcg                                                                                             <  2:56563/266‑1 (MQ=255)
                                                                                                                                                                                                           tCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAg                                                                      >  2:1459171/1‑289 (MQ=255)
                                                                                                                                                                                                           tCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAg                                                                      >  1:225044/1‑289 (MQ=255)
                                                                                                                                                                                                           tCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAg                                                                      >  2:863994/1‑289 (MQ=255)
                                                                                                                                                                                                            ccACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAg                                                                      >  1:1173809/1‑288 (MQ=255)
                                                                                                                                                                                                            ccACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGt                                                                     <  1:737081/289‑1 (MQ=255)
                                                                                                                                                                                                            ccACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGt                                                                     <  1:1877590/289‑1 (MQ=255)
                                                                                                                                                                                                                gTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGa                                                                                                                                                     <  2:1648007/205‑1 (MQ=255)
                                                                                                                                                                                                                   ccAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGcc                                                              >  2:823994/1‑289 (MQ=255)
                                                                                                                                                                                                                   ccAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGc                                                               <  1:1507857/288‑1 (MQ=255)
                                                                                                                                                                                                                     aGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgtta                                                                                          <  2:1886134/259‑1 (MQ=255)
                                                                                                                                                                                                                     aGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCgg                                                            >  2:690461/1‑289 (MQ=255)
                                                                                                                                                                                                                       aCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCgg                                                            >  1:1131413/1‑287 (MQ=255)
                                                                                                                                                                                                                          aaGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGa                                                       <  2:180019/289‑1 (MQ=255)
                                                                                                                                                                                                                           aGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTa                                                                                                                                                               >  2:439362/1‑184 (MQ=255)
                                                                                                                                                                                                                           aGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCg                                                             >  1:470226/1‑282 (MQ=255)
                                                                                                                                                                                                                                 agtgacAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTAGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGtt                                                >  1:1752397/6‑289 (MQ=255)
                                                                                                                                                                                                                                     acAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGAtt                                                     >  2:509621/2‑280 (MQ=255)
                                                                                                                                                                                                                                     acAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATgg                                            >  2:404777/2‑289 (MQ=255)
                                                                                                                                                                                                                                       aGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGgag                                          >  2:2177774/1‑289 (MQ=255)
                                                                                                                                                                                                                                            gctgAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGt                                                 >  1:781285/1‑277 (MQ=255)
                                                                                                                                                                                                                                             ctgAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTAtt                                                                                   <  2:118956/242‑1 (MQ=255)
                                                                                                                                                                                                                                                                tAAAGGTCCGTGCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTTTACATGGGGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGAc                  <  2:2128098/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ccGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTATCAGa          <  1:2104223/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGa                                                           >  2:1679990/1‑239 (MQ=255)
                                                                                                                                                                                                                                                                        cGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGa                                                           <  1:1679990/239‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cGTTCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTATCAGAc         >  1:1890936/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          ttCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCAc                            <  1:1035603/268‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ttCAGTTAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTATCAGAc         >  1:1610418/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                               ttAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGAtt                                                     <  1:1346763/238‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ttAGCTGCGA‑CATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGAtt                                                     >  2:1346763/1‑238 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                        
TGCTTACGGCTAAGATTCGCCGCAAGGTAAGGGCGATGCAGACGCACAGGCAGTCGCGGCTGGCGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAAGCTGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGACTCAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGGCT‑CATATTCATGAAATCAATGGTTATACATGGCGTGCTTTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTATCAGACGCTGATT  >  CP042583/781689‑782248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A