Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,932,428 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,932,4280GC98.9% 605.1 / ‑2.3 264intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base C (131/127);  minor base T (2/1);  total (133/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
*CP0425831,932,4290CG99.2% 618.9 / NA 264intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (133/128);  total (133/131)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,932,4300TA100.0% 634.1 / NA 264intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (133/131);  total (133/131)

Only 100 of 264 total aligned reads displayed.

GACGCGCACCTGTGGCGTAGAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTGC‑TAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAAACTCTGGCGACTCTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTAACTGGCTTGTAAGTCAA  >  CP042583/1932157‑1932707
                                                                                                                                                                                                                                                                               ||||                                                                                                                                                                                                                                                                                     
     gcACCTGTGGCGTAGAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTgg                                                                                                                                                                                                                                                                    >  1:2017237/1‑288 (MQ=255)
                   gAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCg                                                                                                                                                                                                                                                     >  1:142144/1‑289 (MQ=255)
                   gAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCg                                                                                                                                                                                                                                                     >  2:1549399/1‑289 (MQ=255)
                         aaTCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTca                                                                                                                                                                                                                                               >  2:92474/1‑289 (MQ=255)
                         aaTCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTca                                                                                                                                                                                                                                               >  1:1305146/1‑289 (MQ=255)
                                   cGAACACCGCCGCCTAAAAGTTTATAAACCCGAAGCCCGGCCACTTTGCCGCACAGAACCCACAGAGCCAGATCGAAACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCAATGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACg                                                                                                                                                                                                                                     <  2:1054875/289‑1 (MQ=255)
                                      aCAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGAACAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTCAAGATGTTTTTCGACAATAAAACAGCCCATTTCCCCGGCTGTCG‑AAACGGCGAATCCGCTCTGGCCGTTCTCTGCTTCGAATTCAACAACCAACGtt                                                                                                                                                                                                                                   >  2:2242019/1‑287 (MQ=255)
                                         gcgcCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCaa                                                                                                                                                                                                                               >  1:587807/1‑289 (MQ=255)
                                               ccTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGt                                                                                                                                                                                                                         >  2:1141728/1‑289 (MQ=255)
                                                    aaGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGAACAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAAt                                                                                                                                                                                                                     >  1:1971937/1‑288 (MQ=255)
                                                                    aGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCgt                                                                                                                                                                                                     <  1:1549399/288‑2 (MQ=255)
                                                                     acccGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGTTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACAAATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCgtga                                                                                                                                                                                                   <  1:1944182/288‑4 (MQ=255)
                                                                       cggACCACTTTGCCGAACAGATCCCACAAAGCAAGGTCGACACAAGAAATCGCATTAATCACCATGCCACCGAGGCCGAAGAAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGAGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCgtga                                                                                                                                                                                                   <  2:1600938/285‑4 (MQ=255)
                                                                        cGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCtt                                                                                                                                                                                                                                                       <  2:943227/234‑1 (MQ=255)
                                                                                 tGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATc                                                                                                                                                                                       >  1:302477/1‑289 (MQ=255)
                                                                                  gCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATcg                                                                                                                                                                                      <  2:633741/289‑1 (MQ=255)
                                                                                           aTCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTAttt                                                                                                                                                                              >  2:2182992/1‑288 (MQ=255)
                                                                                            tCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTAt                                                                                                                                                                                <  1:44325/285‑1 (MQ=255)
                                                                                            acccACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTAGATATCTCTGACACATTTACCCGCAAAGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTa                                                                                                                                                                             <  1:458130/287‑1 (MQ=255)
                                                                                             cccACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTAc                                                                                                                                                                            >  1:1263010/1‑288 (MQ=255)
                                                                                             cccACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGAAATCGCGGTATTTAc                                                                                                                                                                            >  1:1677944/1‑288 (MQ=255)
                                                                                                acaGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCAt                                                                                                                                                                        <  1:92474/289‑1 (MQ=255)
                                                                                                   gagCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCgg                                                                                                                                                                     >  1:1568411/1‑289 (MQ=255)
                                                                                                     gCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCgggg                                                                                                                                                                   >  1:857609/1‑289 (MQ=255)
                                                                                                     gCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCgggg                                                                                                                                                                   >  2:697252/1‑289 (MQ=255)
                                                                                                     gCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCggg                                                                                                                                                                    <  1:276981/288‑1 (MQ=255)
                                                                                                       cAGATCGACACAAGAAATCGTATTCATAACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGt                                                                                                                                                                  <  1:799572/288‑1 (MQ=255)
                                                                                                           tCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATGTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCa                                                                                                                                                              <  2:2001914/288‑1 (MQ=255)
                                                                                                              acacAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATcgc                                                                                                                                                                                     <  1:1731559/262‑1 (MQ=255)
                                                                                                              acacAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAatat                                                                                                                                                          >  1:947024/1‑289 (MQ=255)
                                                                                                               accaAGAAACCGTATTCATCACCAGGCCACCAGGGCCGGAGTAATACAGGGTGGCACGGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTAAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATg                                                                                                                                                         <  2:290321/287‑1 (MQ=255)
                                                                                                                 caAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGAt                                                                                                                                                       <  2:556925/289‑1 (MQ=255)
                                                                                                                            aTTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGa                                                                                                                                                                                                                                                    >  2:398459/1‑185 (MQ=255)
                                                                                                                                atcaCCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACt                                                                                                                                                                           >  1:1619742/1‑254 (MQ=255)
                                                                                                                                 tcaCCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGAtt                                                                                                                                       <  1:384405/289‑1 (MQ=255)
                                                                                                                                       ggCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGccc                                                                                                                                  >  1:1517037/1‑288 (MQ=255)
                                                                                                                                         ccaccaGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGcccc                                                                                                                                 <  2:1945726/287‑1 (MQ=255)
                                                                                                                                             caGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTc                                                                                                                           >  1:763918/1‑289 (MQ=255)
                                                                                                                                                      aGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATc                                                                                                                  >  1:2187791/1‑289 (MQ=255)
                                                                                                                                                                ggTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATc                                                                                                                                                                                       >  2:895499/1‑210 (MQ=255)
                                                                                                                                                                  tGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGt                                                                                                                                                    <  2:164244/243‑1 (MQ=255)
                                                                                                                                                                  tGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGGTAATGCCAAATGACTGGCGTGACTGCCCGGAATCGCGGTATTTACTCATCGGGGTGGCAATATGAACGTCTATCCAGTGATTCGCCCCCCGGTCGTGATAATCACCGCCGCCAga                                                                                                      >  2:953490/1‑288 (MQ=255)
                                                                                                                                                                     cACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAg                                                                                                                                                                                                                              <  2:800118/166‑1 (MQ=255)
                                                                                                                                                                     cACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAgcgc                                                                                                    >  2:819594/1‑288 (MQ=255)
                                                                                                                                                                     cACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAgcg                                                                                                     >  1:249209/1‑287 (MQ=255)
                                                                                                                                                                     cACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCAACTCCAACAACCAACGTGCAAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAACCGCGGTATTTCCTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCTTGTTCGTGCTAATCACCCCCGCCAgctc                                                                                                    >  2:2118693/1‑286 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGcccc                                                                                                                                 >  1:427253/1‑254 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGcccc                                                                                                                                 <  2:427253/254‑1 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCTTGGTCGTGATAAACACCGCCGCCAGCCCCttt                                                                                                >  2:1770437/1‑287 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTg                                                                                                                        >  2:1743625/1‑263 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTg                                                                                                                        <  1:1743625/263‑1 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAgc                                                                                                      >  1:1014278/1‑281 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGGTATAATCACCCCCCCCACCCCCtttt                                                                                               >  2:2053301/1‑288 (MQ=255)
                                                                                                                                                                          aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCCATCCAGTGATTCGCCCCCTGGTCGTGATAATCCCCGCCCCCCGCCCCttttt                                                                                              >  2:1008580/1‑289 (MQ=255)
                                                                                                                                                                            cATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                                                             <  2:587807/288‑1 (MQ=255)
                                                                                                                                                                                    cGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTc                                                                                                                                      <  2:447875/239‑1 (MQ=255)
                                                                                                                                                                                       ggATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                                                  <  2:1305146/288‑1 (MQ=255)
                                                                                                                                                                                       ggATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                                                  <  2:159166/288‑1 (MQ=255)
                                                                                                                                                                                         aTCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGTGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCg                                                                                >  1:524102/1‑288 (MQ=255)
                                                                                                                                                                                             gTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGt                                                                           >  1:1826573/1‑289 (MQ=255)
                                                                                                                                                                                                        cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTTG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCTTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTTCGTTCGCACCGCCAGTAAACCAGgcg                                                                 >  2:489216/1‑288 (MQ=255)
                                                                                                                                                                                                        cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcgc                                                                >  1:1986417/1‑289 (MQ=255)
                                                                                                                                                                                                        cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcgc                                                                >  1:1699912/1‑289 (MQ=255)
                                                                                                                                                                                                        cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcgc                                                                >  1:720269/1‑289 (MQ=255)
                                                                                                                                                                                                        cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcgc                                                                >  1:47696/1‑289 (MQ=255)
                                                                                                                                                                                                        cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgccc                                                                >  2:1462248/1‑287 (MQ=255)
                                                                                                                                                                                                         tGACACATTTACCCTAAATGAAACGGTTAAGATGTTTTTAGACAATAAAACAGCCCATTTCACCGCCTGTCG‑AAACGGCGAATCCGGTCTGGCAGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGcgcg                                                               <  1:965719/289‑1 (MQ=255)
                                                                                                                                                                                                           acacATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATcg                                                                                                                                                                                      <  2:2139200/168‑1 (MQ=255)
                                                                                                                                                                                                           acacATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATcg                                                                                                                                                                                      >  1:2139200/1‑168 (MQ=255)
                                                                                                                                                                                                            cacaTTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                                                            <  1:1462248/289‑1 (MQ=255)
                                                                                                                                                                                                                   aCCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATcgc                                                                                                                                                                                     >  1:1221542/1‑161 (MQ=255)
                                                                                                                                                                                                                   aCCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCCGTAAACCAGGCGCGAACCTGTTTa                                                     >  1:109952/1‑289 (MQ=255)
                                                                                                                                                                                                                   aCCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTa                                                     >  1:2101310/1‑289 (MQ=255)
                                                                                                                                                                                                                   aCCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTa                                                     >  2:2227510/1‑289 (MQ=255)
                                                                                                                                                                                                                       tCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAttt                                                 >  2:1707881/1‑289 (MQ=255)
                                                                                                                                                                                                                         aaTGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAttttt                                               >  1:2244093/1‑289 (MQ=255)
                                                                                                                                                                                                                           tGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg                                             <  1:819594/289‑1 (MQ=255)
                                                                                                                                                                                                                             aaaCGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa                                           <  1:2227510/289‑1 (MQ=255)
                                                                                                                                                                                                                             aaaCGGTTAAAATGTTTTTCGACAATAAACCAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa                                           <  1:2118693/289‑1 (MQ=255)
                                                                                                                                                                                                                              accGGTTAAGATGTTTTCCGACAATAAAACAGCCCATTTCGCCGGCTGTCG‑AACCGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGt                                            <  2:1568411/285‑1 (MQ=255)
                                                                                                                                                                                                                              aaaggTTAAGATGTTGTTCGACAATAAACCAGCCCATTTCACCGACTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGTGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa                                           <  2:524102/285‑1 (MQ=255)
                                                                                                                                                                                                                                cGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAggg                                        <  1:697252/289‑1 (MQ=255)
                                                                                                                                                                                                                                   ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACcgccgc                                                                                                          >  2:1735974/1‑220 (MQ=255)
                                                                                                                                                                                                                                   ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCa                                     >  1:1776622/1‑289 (MQ=255)
                                                                                                                                                                                                                                         tggtttaCGACAATAAACCAGCCAAATTCACCGGC‑GTACGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGt                               <  2:316057/286‑1 (MQ=255)
                                                                                                                                                                                                                                          ttttttCGACAAAACAACAGCCCATTTCACCGGCTGTCG‑AAACCGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGtt                              <  2:25883/288‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGtt                              <  2:142144/289‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTTTTCGACAAGAAAACAGCCCATTGCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGTACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGtt                              <  1:1768353/289‑1 (MQ=255)
                                                                                                                                                                                                                                             tttCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTtct                            >  1:1600571/1‑288 (MQ=255)
                                                                                                                                                                                                                                             tttCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTtct                            >  1:1340035/1‑288 (MQ=255)
                                                                                                                                                                                                                                               tCGACAATAAAACAGCCCATTTCACCGTCTGTCG‑AAACGGCGAATCCGGTCTGGCAGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCa                         <  1:2240302/289‑1 (MQ=255)
                                                                                                                                                                                                                                               tCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCATCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCa                         <  2:1982797/289‑1 (MQ=255)
                                                                                                                                                                                                                                               tCGACAATAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCa                         <  2:720269/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     aTAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                                                  <  1:1882888/226‑1 (MQ=255)
                                                                                                                                                                                                                                                     aTAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                                                  >  2:1882888/1‑226 (MQ=255)
                                                                                                                                                                                                                                                     aTAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGtt                              <  2:707926/278‑1 (MQ=255)
                                                                                                                                                                                                                                                     aTAAAACAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGtt                              >  1:707926/1‑278 (MQ=255)
                                                                                                                                                                                                                                                       aaaaCAGCCCATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACAAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTatg                 <  2:1776622/289‑3 (MQ=255)
                                                                                                                                                                                                                                                              cccATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTTATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGAGATTCGCCCCCTGGTCGTGATAATCACCGACGCCAGCTCCTTTTTTTGATGTCGCCACGCCAGTAAAACAGCAGCGAACCTGTTTACTTTTTGGTCGGGTCCGGACGTGCTTCCTTGTTaataggtttg          >  2:1489804/1‑281 (MQ=255)
                                                                                                                                                                                                                                                              cccATTTCACCGGCTGTCG‑AAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGCTTCGCCCCCTGGTCGTGATAATCACCGCCGCCCGCGCCTTTTTCTGCCGTCGCCCCGCCCGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGCTGTTCTCCATTGttctgaggcttg          >  2:1229180/1‑279 (MQ=255)
                                                                                                                                                                                                                                                                               ||||                                                                                                                                                                                                                                                                                     
GACGCGCACCTGTGGCGTAGAACTGAATCTCATCACGAACAGCGCCGCCTAAAAGTTTATAAACCGGAAGCCCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTGC‑TAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAAACTCTGGCGACTCTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTAACTGGCTTGTAAGTCAA  >  CP042583/1932157‑1932707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A