Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,968,497 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,968,4970GC99.4% 765.3 / NA 325intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (156/167);  total (156/169)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,968,4980CG99.7% 773.6 / NA 325intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (156/168);  total (156/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,968,4990TA99.1% 766.7 / NA 325intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (156/166);  minor base G (0/3);  total (156/169)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

CCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAAGCTGTATTTGTTGTTACTACTCCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGGCTGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCGCTGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGGCTACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGACTTATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGA  >  CP042583/1968220‑1968778
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                       
     gcCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATc                                                                                                                                                                                                                                                                            <  1:1974388/288‑1 (MQ=255)
        accagGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGt                                                                                                                                                                                                                                                                        <  1:1732454/289‑1 (MQ=255)
         ccacGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAg                                                                                                                                                                                                                                                                         >  1:2030652/1‑287 (MQ=255)
         ccacGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTCCGGGATCCAg                                                                                                                                                                                                                                                                         >  2:2006985/1‑287 (MQ=255)
              cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTcttctt                                                                                                                                                                                                                                                                  >  1:1330287/1‑289 (MQ=255)
                 aTCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTaa                                                                                                                                                                                                                                                               >  1:793926/1‑289 (MQ=255)
                  tCAAGGAAAGCCCGGGGAACACGTCGCAGCGCAGGGGCTGCGCAGAAGACCGCATGCCCACCAGCAACGAGGATTTGTTGTTACTTGACCAGCCAGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTGCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGTCGCGTTCCGAGAATTTCGGGATCCAGTCTTCTTTaaa                                                                                                                                                                                                                                                              <  2:1396842/289‑1 (MQ=255)
                    aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGa                                                                                                                                                                                                                                                            <  2:32333/289‑1 (MQ=255)
                        aaaGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACa                                                                                                                                                                                                                                                         <  1:260696/288‑1 (MQ=255)
                              atggaaaCACTTGATAGCTCAGGATCTGCGCAGAAGACCGAGTCCCACCCGGCAACCGGTATTTGTTGTTACTTGACCAGCACGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGAt                                                                                                                                                                                                                                                  <  2:231702/284‑1 (MQ=255)
                                        cgctcagcaacgggTCAGCGCAGAAGCACACATCGAACCCATCAACGAGTATTAGTTGCTACTTGACCAGCCCGAAAACCGCACCGCGTAAACCGCCAGCCCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCa                                                                                                                                                                                                                                        <  2:113250/278‑1 (MQ=255)
                                          cGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCaa                                                                                                                                                                                                                                       >  1:1000110/1‑288 (MQ=255)
                                               cTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCa                                                                                                                                                                                                                                    >  1:955567/1‑286 (MQ=255)
                                               cTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCa                                                                                                                                                                                                                                    >  1:1139563/1‑286 (MQ=255)
                                                  aGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGa                                                                                                                                                                                                                              >  1:1877165/1‑289 (MQ=255)
                                                     gTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGa                                                                                                                                                                                                                              <  1:601258/286‑1 (MQ=255)
                                                           gcAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAgcgaaccgc                                                                                                                                                                                                                     <  1:645344/289‑9 (MQ=255)
                                                            cAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGcc                                                                                                                                                                                                                    <  1:1577800/289‑1 (MQ=255)
                                                             aGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAAAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGcc                                                                                                                                                                                                                    <  2:766060/288‑1 (MQ=255)
                                                              gAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCACCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGGAAAGGCAATCATCGGTGCCAGGGTAAAGATTACGCGAACCGAGAATTTCGGGATCCAGTCTCCTTTAAAGAACATTTCGATCCTGTCCACAACCAGCtcacccgacccgccc                                                                                                                                                                                                                   >  2:2175181/1‑274 (MQ=255)
                                                                aGCACGCATCGCACCCAGCAACGAGTTTTTGTTGTTACCTGACCAGCCCGCAAACAGCACCGCTTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGcccc                                                                                                                                                                                                                  <  1:1671905/287‑1 (MQ=255)
                                                                    cGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                                                                             >  2:1314921/1‑288 (MQ=255)
                                                                    cGCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACAGCCCCAGCCa                                                                                                                                                                                                             >  1:1899108/1‑288 (MQ=255)
                                                                     gCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAac                                                                                                                                                                                                           <  1:1457472/289‑1 (MQ=255)
                                                                     gCATCGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAac                                                                                                                                                                                                           <  2:103582/289‑1 (MQ=255)
                                                                         cGCACCCAGCAACGAGTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACAtt                                                                                                                                                                                                                                                       >  2:1922331/1‑241 (MQ=255)
                                                                           caccccgcacaggttATTTGTTGTTACTTGACCAGCCCGCAAACGGACCCGCGTAAACCGCCAGACCGGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGtt                                                                                                                                                                                                     <  2:2030652/275‑1 (MQ=255)
                                                                            acccaggaacggatATTTGTTGTAACTTGACCAGCACGCAAACAGCACCGCGTAAGCCGCCAGACCTGCCATCATCATGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGAATGACTGGCACAATCGCAAAGGCCAGCATCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGtt                                                                                                                                                                                                     <  1:354431/275‑1 (MQ=255)
                                                                            aCCCAGCAACGAGAATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTa                                                                                                                                                                                                    >  1:1826314/1‑289 (MQ=255)
                                                                                  caacgagTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGt                                                                                                                                                                                                                                                                        <  2:1286952/209‑1 (MQ=255)
                                                                                  caacgagTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGt                                                                                                                                                                                                                                                                        >  1:1286952/7‑215 (MQ=255)
                                                                                     cgagTAGTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTaa                                                                                                                                                                                           <  2:1092861/286‑1 (MQ=255)
                                                                                      gaggaTTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACACGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCGTCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  1:195769/285‑1 (MQ=255)
                                                                                      gagTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  1:865070/287‑1 (MQ=255)
                                                                                      gagTATTTGTTGTTACTTGAACAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCCTCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  1:290481/287‑1 (MQ=255)
                                                                                      cattATTTGTTGTTAATTGACCAGCCCGCAACCAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCCACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  1:54283/286‑1 (MQ=255)
                                                                                       ataagttgttgTGACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACAACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  2:657131/283‑1 (MQ=255)
                                                                                       ataaTTTGTTGTTACATGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCGGCCATCATCAGGAAGAACAAAATCCCGAGGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCAAAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  2:369757/285‑1 (MQ=255)
                                                                                        gTATTTGTTGTTACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACgg                                                                                                                                                                                        >  2:1218162/1‑289 (MQ=255)
                                                                                               ttgttACTTGACCAGCCCGCAAACAGCACCGCGGAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAAACACCCAACCCGGACTGACGGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGa                                                                                                                                                                                                                                                            <  1:1416751/214‑1 (MQ=255)
                                                                                                tgttACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                                                                                                                                                                                <  1:472614/289‑1 (MQ=255)
                                                                                                   tacttaaccAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTaa                                                                                                                                                                                           <  1:2135223/268‑1 (MQ=255)
                                                                                                       tgaccAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACCGTTCTGGAACAGAccc                                                                                                                                                                         <  2:840390/286‑1 (MQ=255)
                                                                                                         accAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCca                                                                                                                                                                        >  2:1982286/2‑288 (MQ=255)
                                                                                                            aGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCcagcag                                                                                                                                                                    >  1:2257001/1‑289 (MQ=255)
                                                                                                              cccGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCa                                                                                                                                                                                                                                                >  2:2097113/1‑211 (MQ=255)
                                                                                                              cccGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCAGCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCa                                                                                                                                                                                                                                                <  1:2097113/211‑1 (MQ=255)
                                                                                                                   aaaCAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCcag                                                                                                                                                                       <  1:427034/279‑1 (MQ=255)
                                                                                                                         gcccGCGTAAACCGCCATACCTGCCAACATCAGGAAGAACAAAATCCCGACTTGCAGTTCGGGAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGTTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACTTTCGCCa                                                                                                                                                       <  2:1689551/287‑1 (MQ=255)
                                                                                                                         cACCGCGTAAACCCCCAGACCGGCCATCATCAGGAAAAACAAAATCCCGATGTTCAGGTCGGCAACCCCCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                                                                                                                       <  2:1877165/289‑1 (MQ=255)
                                                                                                                         aaCCCCGTAAACCGCAAGACCTGCCATCATCAGGAAGAACAAAATCCCGAGGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                                                                                                                       <  1:664301/288‑1 (MQ=255)
                                                                                                                          aCCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGACCAAAATCCCGAGGTTCAGGTCGGCAACCACCCAACCCGGACGGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                                                                                                                       <  2:733909/288‑1 (MQ=255)
                                                                                                                            cgcctcaccagCCAGACCTGCCATCATCAGGAAGACCAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAg                                                                                                                                                    <  1:55452/279‑1 (MQ=255)
                                                                                                                              cgTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          >  2:145238/1‑249 (MQ=255)
                                                                                                                              cgTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGc                                                                                                                                                   >  1:1185486/1‑288 (MQ=255)
                                                                                                                                        cAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGcc                                                                                                                                        <  1:830587/289‑1 (MQ=255)
                                                                                                                                        cAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGcc                                                                                                                                        <  1:2006985/289‑1 (MQ=255)
                                                                                                                                        cAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGc                                                                                                                                         >  2:2060089/1‑288 (MQ=255)
                                                                                                                                               gCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGATTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATCTCGGGATCCAGTCTTCTTTAAAGACAATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTAAGGTCAGGAACGGTTCTGGAACAGACCCAGCAGCCGACGTTCGCCAAAGCTCATGaacggcacgaagg                                                                                                                                 >  1:1608732/1‑278 (MQ=255)
                                                                                                                                                  atcatcAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGa                                                                                                                              <  1:1059538/289‑1 (MQ=255)
                                                                                                                                                          gaagaaCAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAaccacc                                                                                                                      >  1:843988/1‑289 (MQ=255)
                                                                                                                                                              aaCAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagca                                                                                                                  >  1:1938546/1‑289 (MQ=255)
                                                                                                                                                               aCAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc                                                                                                                   <  2:1997909/287‑1 (MQ=255)
                                                                                                                                                                       ccGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca                                                                                                         <  2:194439/289‑1 (MQ=255)
                                                                                                                                                                        cGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  1:1997218/207‑1 (MQ=255)
                                                                                                                                                                          aTGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                                                                          <  2:1589066/205‑1 (MQ=255)
                                                                                                                                                                                   tgcgcaacacaccAACCCGGACTGAATGGCCAAAGATCAAAGGCCAGGAGGAGCGAGGTAAAGGCAATAAGCGGTGACGGGGAAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTGTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAgg                                                                                              <  2:2131182/277‑1 (MQ=255)
                                                                                                                                                                                     ggCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAgg                                                                                                                >  1:1787023/1‑268 (MQ=255)
                                                                                                                                                                                            accCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcagca                                                                                    <  1:1311570/289‑1 (MQ=255)
                                                                                                                                                                                             cccAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                                                                                                                       <  1:754383/221‑1 (MQ=255)
                                                                                                                                                                                              ccAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAgg                                                                                  <  1:1218162/289‑1 (MQ=255)
                                                                                                                                                                                                 aCCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGTAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAt                                                                                >  2:1556574/1‑288 (MQ=255)
                                                                                                                                                                                                 aCCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca                                                                                                         >  2:611885/1‑263 (MQ=255)
                                                                                                                                                                                                       agcgactgGCACAACACCAAGGGCCAGCAGCAGCGAAGTTAATGCAATAGTCGTTGCCAGGGGAAAGATGACCGGGTCCGAGAATTTCGGGATCCAGTCTGCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGTGCGAACCGCCCCAGCCAACAAGGTTAGGTCCGTAACGGTTCTTGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAt                                                                          <  2:1180118/285‑1 (MQ=255)
                                                                                                                                                                                                       actgactgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAt                                                                          <  1:704163/288‑1 (MQ=255)
                                                                                                                                                                                                       actgactgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAt                                                                          <  2:542851/288‑1 (MQ=255)
                                                                                                                                                                                                           actgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                                                                                                                                                                                >  1:2057434/1‑182 (MQ=255)
                                                                                                                                                                                                             tggaaaaaccggaaaGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTc                                                                    <  2:2241848/276‑1 (MQ=255)
                                                                                                                                                                                                                 acaATCACAACGGCCAGCAGCAGCGAGGGAAAGGCAATACTCGGTGCCAGGGTAAAGATGACGCAATCCGAGACGTTCGGGATCCAGTCTTCTTTAAAGAACCTTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCggtg                                                               <  2:418488/289‑3 (MQ=255)
                                                                                                                                                                                                                     tCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                                                                                                                       <  1:1027536/197‑1 (MQ=255)
                                                                                                                                                                                                                     tCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAtc                                                                               >  1:1502900/1‑269 (MQ=255)
                                                                                                                                                                                                                     tCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCggtgata                                                            >  2:1350805/1‑283 (MQ=255)
                                                                                                                                                                                                                          aaGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGcc                                                                                                                                        <  1:514628/207‑1 (MQ=255)
                                                                                                                                                                                                                             gCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACATCCACCAGCAGGATCACCACCGCTTTGATGATGGCCAGCATGATCTCAAACAGTTCCGGTGATATCCAAATCa                                                   >  1:1379567/1‑289 (MQ=255)
                                                                                                                                                                                                                                      ggcgaggTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTtgtg                                           <  1:1047575/283‑1 (MQ=255)
                                                                                                                                                                                                                                      agcgaggTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTtgtg                                           <  1:730408/283‑1 (MQ=255)
                                                                                                                                                                                                                                      agcgaggTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTtgtg                                           <  2:496536/283‑1 (MQ=255)
                                                                                                                                                                                                                                      agcgaggTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTtgtg                                           <  2:888066/283‑1 (MQ=255)
                                                                                                                                                                                                                                                agtccatcatcGGGTCCAGGGTAAAGATGCCGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTgaga                                <  2:1022025/284‑1 (MQ=255)
                                                                                                                                                                                                                                                     atcatcGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAac                                                                                                                          <  1:28246/194‑1 (MQ=255)
                                                                                                                                                                                                                                                      tcatcGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCa                          >  2:1044200/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tcatcGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCa                          >  1:2237257/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tcatcGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGAGATATCCAACTCATTGCTGTGCCTCCTTGAGATCCTCa                          >  2:924616/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           ggTGCCAGGGGAAAGAAGACGCGATCCGAGAATTTCAGGCTCCAGTCTTCTTTCAAGAACATTTTGATCATGTCCGCAAACAGCTGGAGCGACCCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGAt                      <  2:1744658/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            gTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcagca                                                                                    <  2:1788866/225‑1 (MQ=255)
                                                                                                                                                                                                                                                                cAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGATGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAATATgcgcg                 >  1:1770813/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   ggTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATgcgcg                 >  2:691141/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                       aaGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca                                                                                                         >  1:17078/1‑193 (MQ=255)
                                                                                                                                                                                                                                                                       aaGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCgccagcca          >  2:520344/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                              cgcgATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTc                           >  1:1117626/1‑264 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                       
CCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAAGCTGTATTTGTTGTTACTACTCCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGGCTGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCGCTGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGGCTACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGACTTATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGA  >  CP042583/1968220‑1968778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A