Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F210 I1 R1	4928	385.2	6658600	97.5%	6492135	231.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	607,662	3 bp→TCG	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	607,662	0	A	T	99.5%	868.4 / NA	364	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (177/185); minor base C (2/0); total (179/185)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*	CP042583	607,663	0	G	C	99.2%	856.5 / NA	364	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (3/0); new base C (176/185); total (179/185)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*	CP042583	607,664	0	C	G	99.5%	859.5 / ‑3.6	364	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (1/0); major base G (176/184); minor base . (2/0); total (179/185)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 364 total aligned reads displayed.

TAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCAGTTGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAAAGCAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCAGCCAGGATGCATTCTGGCGTAGCCCCTGGCAGCTTAG‑CAGCCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTAGTCTGGAAGAGGC  >  CP042583/607382‑607939

|||

tAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTgg                                                                                                                                                                                                                                                                                <  1:2802786/289‑1 (MQ=255)

 aaaCGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCCGGATGCATTCTGGCGTTCGCCCTgg                                                                                                                                                                                                                                                                                >  1:2972070/1‑288 (MQ=255)

                             atgtGGCGGTCGCGGTGTGTAATGATGTTCCGCCGGGAATGGGCTCATGCAGCACCTGTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGTATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGGTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCGTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATggg                                                                                                                                                                                                                                                  <  2:2353340/286‑1 (MQ=255)

                                     gTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGTTGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGCCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCCTGAGTTCGATCGCGCCTCGCAGGATGCATTCTGTCGTTCGCACTGGCTGCTTAGCCAGCC‑AAGTAAcccccctggcgaacgc                                                                                                                                                                                                                                           >  2:921583/1‑274 (MQ=255)

                                     gTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCTGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAACGTAACCGCATGGGCGTTCGCt                                                                                                                                                                                                                                          >  2:2253409/1‑289 (MQ=255)

                                     gTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTag‑ct                                                                                                                                                                                                                                                                     >  1:831202/1‑261 (MQ=255)

                                       cgcgGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATggg                                                                                                                                                                                                                                                  >  1:2905962/1‑279 (MQ=255)

                                         cgGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CCCGCAAAGGTACCGCATTGGCCATCGCTTCCa                                                                                                                                                                                                                                      >  2:2853423/1‑289 (MQ=255)

                                                       gTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTCTGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTTGCACAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTCTaa                                                                                                                                                                                                                        >  2:291808/1‑289 (MQ=255)

                                                          ccgccgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAATTAACCGCCTGGGCTATCGCTTACCGGGGCAAATTTTAAAAc                                                                                                                                                                                                                     >  2:1214837/1‑289 (MQ=255)

                                                          ccgccgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTa                                                                                                                                                                                                                         <  2:2022461/285‑1 (MQ=255)

                                                                       gctcatGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATc                                                                                                                                                                                                         <  2:2183111/283‑1 (MQ=255)

                                                                         tcatGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATcgcg                                                                                                                                                                                                      <  1:2122646/286‑1 (MQ=255)

                                                                         tcatGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATcgc                                                                                                                                                                                                       <  1:921583/285‑1 (MQ=255)

                                                                           atGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCa                                                                                                                                                                                                                                                      >  1:1322516/2‑239 (MQ=255)

                                                                           atGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGaa                                                                                                                                                                                                    <  1:1983337/288‑1 (MQ=255)

                                                                           atGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGaa                                                                                                                                                                                                    <  1:3195114/288‑1 (MQ=255)

                                                                                     gATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTAtct                                                                                                                                                                                           >  1:2205107/1‑288 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCa                                                                                                                                                                                                                                                      >  2:1069727/1‑228 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCa                                                                                                                                                                                                                                                      >  1:2514992/1‑228 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATggg                                                                                                                                                                                                                                                  >  1:1840217/1‑232 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATggg                                                                                                                                                                                                                                                  <  2:1840217/232‑1 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTa                                                                                                                                                                                                                         >  1:155697/1‑257 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTa                                                                                                                                                                                                                         <  2:155697/257‑1 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATc                                                                                                                                                                                                         >  2:753164/1‑273 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTAtctc                                                                                                                                                                                          >  1:640339/1‑288 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTAtctc                                                                                                                                                                                          >  1:738577/1‑288 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTAtctc                                                                                                                                                                                          >  1:2636731/1‑288 (MQ=255)

                                                                                      aTCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTAtctc                                                                                                                                                                                          >  1:368073/1‑288 (MQ=255)

                                                                                       tctcAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCa                                                                                                                                                                                         <  2:462249/288‑1 (MQ=255)

                                                                                               tGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTaaa                                                                                                                                                                                                                       <  1:3278581/250‑1 (MQ=255)

                                                                                                ggggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCCCACCGGTTGTTa                                                                                                                                                                               >  2:639031/1‑289 (MQ=255)

                                                                                                 gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTa                                                                                                                                                                               >  1:30108/1‑288 (MQ=255)

                                                                                                 gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCCATCGCTAACTGTTTTCCCACGGTTTGTTa                                                                                                                                                                               >  2:2894210/1‑288 (MQ=255)

                                                                                                 gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAAAGCACCCACGATCGCGAACTGTTATCCCCCGGTTTGTTa                                                                                                                                                                               >  2:1266605/1‑288 (MQ=255)

                                                                                                  ggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATCTTAAAACGCACCCCCGATCGCGAACTGTGATCTCATGGTTTGTTAcc                                                                                                                                                                             >  2:701696/1‑289 (MQ=255)

                                                                                                   gATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTAcc                                                                                                                                                                             <  2:398733/288‑1 (MQ=255)

                                                                                                     ttGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCggg                                                                                                                                                                          <  2:2636731/289‑1 (MQ=255)

                                                                                                              tGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCgtggt                                                                                                                                                                    >  2:1023996/1‑286 (MQ=255)

                                                                                                              gtgAAGGCCGTTGACGAAAGGATGGGTCACGACTCACGCTTGG‑GAAAGCAAGGCGTGATTCTATCGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCGGGGATATCATGAGTTCGACCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAACGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTACAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGc                                                                                                                                                                  <  2:1316389/286‑1 (MQ=255)

                                                                                                                      cGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCca                                                                                                                                                          >  2:1414873/1‑288 (MQ=255)

                                                                                                                        tttACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGAACGCGAACTGTTAACTCCCGGTTTGTTACCGGGCGGGGTTCCGTTGCcacca                                                                                                                                                       >  2:2720093/1‑287 (MQ=255)

                                                                                                                               aaGGATGGTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCAGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGc                                                                                                                                                >  1:2249104/1‑289 (MQ=255)

                                                                                                                                      gTGACCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTa                                                                                                                                                                                                                         >  2:2511527/1‑209 (MQ=255)

                                                                                                                                         aCCGACTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTa                                                                                                                                                                                                                                                            >  1:5380/1‑171 (MQ=255)

                                                                                                                                             aCTCCCGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTa                                                                                                                                                                                                                                                            <  2:2551795/167‑1 (MQ=255)

                                                                                                                                                  cGATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGAACGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGaa                                                                                                                             <  1:2281017/289‑1 (MQ=255)

                                                                                                                                                   gATTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAac                                                                                                                            <  2:824443/289‑1 (MQ=255)

                                                                                                                                                    aTTGGCAAATCGAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATcgcg                                                                                                                                                                                                      <  2:1342057/214‑1 (MQ=255)

                                                                                                                                                       ggcaaatcgaa‑gcGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTa                                                                                                                                                                                                                         <  2:3219082/183‑1 (MQ=255)

                                                                                                                                                        gcaaatcgaa‑gcGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATTAacgacg                                                                                                                        >  2:1461789/9‑288 (MQ=255)

                                                                                                                                                          aaatcgaa‑gcGTGATTCTACGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCATCCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGca                                                                                                                      <  1:1379989/282‑1 (MQ=255)

                                                                                                                                                                        aTTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCggtgg                                                                                                        >  1:1008518/1‑289 (MQ=255)

                                                                                                                                                                        aTTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGATTGTTACCGGGCGTGGTGCAGGTACCACATTACGGGCAGCCGGTTGTGTTGATGAAAGACGCACAGACCCCtggtgg                                                                                                        >  2:836777/1‑289 (MQ=255)

                                                                                                                                                                            tATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTTCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTa                                                                                                     >  1:146083/1‑288 (MQ=255)

                                                                                                                                                                              tGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCGTTCGCGCCGTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATGTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAcc                                                                                                   <  1:3038125/288‑1 (MQ=255)

                                                                                                                                                                              tGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTa                                                                                                                                                                                                                                               >  2:2234122/1‑148 (MQ=255)

                                                                                                                                                                                   gcgcAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCgtggt                                                                                                                                                                    >  1:1494484/1‑218 (MQ=255)

                                                                                                                                                                                   gcgcAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATa                                                                                                                                                      <  1:1211260/232‑1 (MQ=255)

                                                                                                                                                                                   gcgcAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGa                                                                                                                                          <  1:1652294/244‑1 (MQ=255)

                                                                                                                                                                                   gcgcAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGa                                                                                                                                          >  2:1652294/1‑244 (MQ=255)

                                                                                                                                                                                        gggCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGcaccac                                                                                                                                                                                                              >  2:2143047/1‑171 (MQ=255)

                                                                                                                                                                                         ggCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCa                                                                                                                                                                                         <  2:2241573/191‑1 (MQ=255)

                                                                                                                                                                                         ggCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGAGCTCGCCCTGGCCGCTTAG‑CCCGCAAAGTAACCCCATGGGCTATCGCTTACAGGGGCAAATTTTAATACGCACCACCGACCGCGACCTGTTATCTCACGGTTTGGTACCGGGCGTGGTGCCGGTGCCACATCACGGGCACGCGATCGTGTTGGTGAACGCCGCACAGCCCACCGGTGTGTACCCTGGCATTGCCt                                                                                       >  2:3038125/1‑289 (MQ=255)

                                                                                                                                                                                          gCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGc                                                                                         <  1:1266605/286‑1 (MQ=255)

                                                                                                                                                                                          gCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGc                                                                                         <  2:3274250/286‑1 (MQ=255)

                                                                                                                                                                                             ttAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTa                                                                                    >  1:881546/1‑288 (MQ=255)

                                                                                                                                                                                              tAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAg                                                                                                                   >  1:2384672/1‑256 (MQ=255)

                                                                                                                                                                                                 aCAGCGGCTGGGGGGCAACCGCATTGGCGCCTTGCCGGGGCAGGAAGATCATGAGTTCGATCGCGCCTCGCAGGATGCAGTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCAtt                                                                               <  2:1913571/289‑1 (MQ=255)

                                                                                                                                                                                                     ctgctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTAcc                                                                                                                                                                             >  1:763012/1‑191 (MQ=255)

                                                                                                                                                                                                     ctgctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCg                                                                                                                                                                        >  2:2046565/1‑196 (MQ=255)

                                                                                                                                                                                                     ctgctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGca                                                                                                                      >  1:109499/1‑246 (MQ=255)

                                                                                                                                                                                                      tggtgtgGGCCAACCGCGTTCGCGGCTTGCCGGGGCTGGGATTTCATGAGTCCGTGCGCGCCTCGCAGGTTGCATACTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCGAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTACCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACCTAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAgg                                                                           <  2:1707531/285‑1 (MQ=255)

                                                                                                                                                                                                      tgctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCa                                                                                                                                               <  2:1159459/220‑1 (MQ=255)

                                                                                                                                                                                                      tgctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCa                                                                                                                                               >  1:1159459/1‑220 (MQ=255)

                                                                                                                                                                                                      tgctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAgg                                                                           <  1:2026297/288‑1 (MQ=255)

                                                                                                                                                                                                      tgatgtgGGGCAACCGCATTCGCGCCGTGCCGGGGTCGGGATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTAGCATTGAgg                                                                           <  1:2077192/285‑1 (MQ=255)

                                                                                                                                                                                                       gctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAg                                                                            >  1:3093601/1‑286 (MQ=255)

                                                                                                                                                                                                        ctgTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAgg                                                                           <  2:3093601/286‑1 (MQ=255)

                                                                                                                                                                                                                cAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGa                                                                                                                                          >  1:1917884/1‑215 (MQ=255)

                                                                                                                                                                                                                  aCCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCgt                                                                                                                                                                       >  1:3236493/1‑184 (MQ=255)

                                                                                                                                                                                                                  aCCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGc                                                                                                                                                                  <  2:2087411/189‑1 (MQ=255)

                                                                                                                                                                                                                  aCCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCa                                                              >  1:448429/1‑289 (MQ=255)

                                                                                                                                                                                                                                          cGGAATATCATGAGTTCGATCGCGCCTCTCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTTGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCTCAGACCACCGGTTGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTc                                       <  1:2557637/288‑1 (MQ=255)

                                                                                                                                                                                                                                           ggAATATGATGAGTTCGGTCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAg                                     <  1:2447345/289‑1 (MQ=255)

                                                                                                                                                                                                                                           ggAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAacg                                                                                                                           >  1:3515/1‑203 (MQ=255)

                                                                                                                                                                                                                                                atCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGAt                                <  2:1970082/289‑1 (MQ=255)

                                                                                                                                                                                                                                                  cATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCa                                                                                                                                               <  2:2999606/176‑1 (MQ=255)

                                                                                                                                                                                                                                                  cATGAGTTCGATCACGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGAtt                               >  2:2436318/1‑288 (MQ=255)

                                                                                                                                                                                                                                                       gTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGac                                                                                                                 <  2:332984/201‑1 (MQ=255)

                                                                                                                                                                                                                                                       gTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCggtggt                                                                                                       >  1:6113/1‑211 (MQ=255)

                                                                                                                                                                                                                                                       gTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCAtttt                         >  1:2153894/1‑289 (MQ=255)

                                                                                                                                                                                                                                                       gTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATAAACGACGCACAGACCACCggtggt                                                                                                       <  2:6113/211‑1 (MQ=255)

                                                                                                                                                                                                                                                          cGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCAtt                                                                               <  1:1047006/232‑1 (MQ=255)

                                                                                                                                                                                                                                                           gATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTg                                                                                      <  1:1738637/224‑1 (MQ=255)

                                                                                                                                                                                                                                                                  cctcgcagGATGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTAcc                                                                                                                                                                             >  1:2237473/6‑130 (MQ=255)

                                                                                                                                                                                                                                                                            tGCATTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAagag    <  1:2720093/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                               aTTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTa                                                                                                                                                                                                                         >  2:2792759/1‑73 (MQ=255)

                                                                                                                                                                                                                                                                               aTTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTAtt                                                                                           <  1:2875455/199‑1 (MQ=255)

                                                                                                                                                                                                                                                                               aTTCTGGCGTTCGCCCTGGCAGCTTAG‑CTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAagag    >  1:143809/1‑286 (MQ=255)

|||

TAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCAGTTGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAAAGCAA‑GCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCAGCCAGGATGCATTCTGGCGTAGCCCCTGGCAGCTTAG‑CAGCCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTAGTCTGGAAGAGGC  >  CP042583/607382‑607939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A