Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 727,883 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583727,8830GC98.2% 618.9 / NA 271intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base C (139/127);  total (139/132)
Fisher's exact test for biased strand distribution p-value = 2.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583727,8840CG99.3% 624.4 / NA 271intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base G (138/129);  total (139/132)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
*CP042583727,8850TA98.2% 625.7 / NA 271intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (138/127);  minor base G (0/5);  total (139/132)
Fisher's exact test for biased strand distribution p-value = 2.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

GTTGATTACGTACCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTGCTGAGGCTAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGGCTGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGACTACCCTGAT  >  CP042583/727612‑728156
                                                                                                                                                                                                                                                                               |||                                                                                                                                                                                                                                                                                
      tagctacgcgaccGTTAGACGGACAGTGGAGCTCAGGATAGGGTTCAGCTATCATGGCAGCACGTTCTGGGTCATCCAGCAGGGCGTTGACTGGCGTAATGGGGTTATTTGGGTTGATCCGGGTCAGGGCGGAACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGgccgcc                                                                                                                                                                                                                                                             <  2:1053824/277‑1 (MQ=255)
      tACGTACCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGCGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCAGAGgccga                                                                                                                                                                                                                                                              >  1:2716218/1‑287 (MQ=255)
         gTACCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAg                                                                                                                                                                                                                                                          >  1:2536354/1‑289 (MQ=255)
         gTACCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAg                                                                                                                                                                                                                                                          >  2:1191237/1‑289 (MQ=255)
          tACCGCAACGTTATCCATACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCCGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAgg                                                                                                                                                                                                                                                         <  1:1248783/289‑1 (MQ=255)
           aCCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAg                                                                                                                                                                                                                                                                   >  1:2843742/1‑278 (MQ=255)
           aCCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAgg                                                                                                                                                                                                                                                         >  1:2657553/1‑288 (MQ=255)
                  cGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGCTACCGCGAGGCTGTCCACCCGAGGCCGCCCAGGCCCACTa                                                                                                                                                                                                                                                  >  2:2844636/1‑288 (MQ=255)
                            aCAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTAGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGccgccga                                                                                                                                                                                                                                       >  1:874924/1‑283 (MQ=255)
                                   aGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTTCGAGGCTGCACAGCCGACGCCGCCCAGGCCCACTAACCGCACGCGAGAATCtt                                                                                                                                                                                                                                >  1:1170531/1‑289 (MQ=255)
                                   aGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGgccgcc                                                                                                                                                                                                                                                             <  2:1280264/260‑1 (MQ=255)
                                   aGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGgccgcc                                                                                                                                                                                                                                                             >  1:1280264/1‑260 (MQ=255)
                                   aGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGATTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTTCAGATTCCAGAGCGAAACCGTTTCGCAGTCGAGCAGCGTCAGCTCACCTACATCGGCGCTTCCCCGCTACTGCACGCCAGCACAGCCGCGGCCACCCCGGCCCACTCACACCCCCCCAGAATCtt                                                                                                                                                                                                                                >  2:1828229/1‑289 (MQ=255)
                                        ggacaggtgtTCAGGAATGAGTGCTGCAAGTGGTGCGTCATACAGGGGTGGATTGACTGGAGTAATCGCGGGAGGTGGGTTGATAGGCGACGGGGCGTCACGGGCGGATGCCACCTTCGGTTGACCGACCGTGGCATAGCTGTGCAGTTTCGGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCGTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAg                                                                                                                                                                                                                            <  2:2032017/282‑1 (MQ=255)
                                        cAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAgc                                                                                                                                                                                                                           <  2:359477/289‑1 (MQ=255)
                                          gATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGCCCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGGGTCGAAGTCGATCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGCCCGCCCAGGCCCCCTATCAGCACGCCACAATCTTCCAgcc                                                                                                                                                                                                                          >  2:911514/1‑287 (MQ=255)
                                                gtTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGgccgcc                                                                                                                                                                                                                                                             <  2:170455/247‑1 (MQ=255)
                                                  tCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTgg                                                                                                                                                                                                                  >  1:1975623/1‑288 (MQ=255)
                                                    aGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCa                                                                                                                                                                                                                                                           <  1:785490/245‑1 (MQ=255)
                                                           aatGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCaaaa                                                                                                                                                                                                         >  1:994706/1‑288 (MQ=255)
                                                                tgcAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTc                                                                                                                                                                                                             >  2:98795/1‑279 (MQ=255)
                                                                   aaGTTCTGCGTCTTCCGCCGGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGCCGGGGGGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCcg                                                                                                                                                                                                <  2:2745090/289‑1 (MQ=255)
                                                                    aGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACt                                                                                                                                                                                                                                                   >  1:2112397/1‑237 (MQ=255)
                                                                       tCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAACCAAAGCCGCGC‑a                                                                                                                                                                                           >  1:1540691/1‑289 (MQ=255)
                                                                         tGCGTCAGCACGCAGTGCATTGCCTGGCGTAATGGCGATATGTGGGTTGATCCGCGTCAGGGCGGCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGa                                                                                                                                                                                         <  2:2929904/289‑1 (MQ=255)
                                                                                tCCAGCAGTGCATTGACTGGCTTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAgg                                                                                                                                                                                                                                                                  <  2:2798381/210‑1 (MQ=255)
                                                                                        tGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTg                                                                                                                                                                          >  1:716853/1‑289 (MQ=255)
                                                                                        tGCATTGACTGGCGGTATTGGGATATGGGGGTGGCCCCGCGTCAGGGCGTCACGGGCGGTTTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGGGAGCGAAACCGCGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTg                                                                                                                                                                          <  2:1621982/289‑1 (MQ=255)
                                                                                          cATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTc                                                                                                                                                                                                             <  2:2649627/253‑1 (MQ=255)
                                                                                          cATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTa                                                                                                                                                                        >  1:3121188/1‑289 (MQ=255)
                                                                                               aCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCgggtttcacgca                                                                                                                                                                   >  2:831297/1‑279 (MQ=255)
                                                                                                  ggCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCgcagca                                                                                                                                                                >  1:2461049/1‑289 (MQ=255)
                                                                                                  ggCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTACCAGCACGCGAGAATCTTTCAGCGCCCCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCtcagcc                                                                                                                                                                >  2:507743/1‑288 (MQ=255)
                                                                                                  ggCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGCGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTc                                                                                                                                                                                                             <  2:2417638/245‑1 (MQ=255)
                                                                                                              atatGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATc                                                                                                                                                      >  1:101167/1‑287 (MQ=255)
                                                                                                              atatGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGc                                                                                                                                                    >  1:3057367/1‑289 (MQ=255)
                                                                                                              atatGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGACTCCCGGCCGTCCAAATCAAAGCC‑CGCAAGAACACGTTGCCGGTTGTAGCCCAGCCTCTCCTGATcc                                                                                                                                                     >  2:2373955/1‑287 (MQ=255)
                                                                                                               tatGGGGGTTGGTCCGCGTCGGGGCGTAACCGCAGGATTTCACCTTCGGTTACCCGACCGTGGCATCAATGTGCAGTGTCTGGCGTTGCAGAGTCGAGAGCGAAACCGTGTCGAATTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGc                                                                                                                                                    <  2:2102056/288‑1 (MQ=255)
                                                                                                                   tgGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGGGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTGTCAGCGCCTCCTGGCCGTCCAAATCAAAGCCGCGC‑ACAATTACTTCCCGGTTGTAGCGCAGCCGCCCCTTATCCCTGAGt                                                                                                                                               >  2:2531082/1‑289 (MQ=255)
                                                                                                                    gggTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGt                                                                                                                                               <  1:287848/288‑1 (MQ=255)
                                                                                                                    gggTGGAGCCGCGCCGGGCGGTCACGGGCGGATGCCACCTTCGGTTGCCCGACCGTGGCATCACTGTCCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGt                                                                                                                                               <  2:598146/288‑1 (MQ=255)
                                                                                                                      gTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTc                                                                                                                                             <  2:1980450/288‑1 (MQ=255)
                                                                                                                        tGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGc                                                                                                                                          <  1:2027310/289‑1 (MQ=255)
                                                                                                                         gATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGc                                                                                                                                          >  2:153303/1‑288 (MQ=255)
                                                                                                                           tCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGaa                                                                                                                                                                                                                                    <  2:3247202/197‑1 (MQ=255)
                                                                                                                           tCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAgc                                                                                                                                                                                                                           >  1:2248334/1‑206 (MQ=255)
                                                                                                                           tCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTCCGAGGCTGCACAGCCGAGGCCGCCCa                                                                                                                                                                                                                                                           >  1:1485882/1‑174 (MQ=255)
                                                                                                                               cgTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCCAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTa                                                                                                                                                                        >  2:2994295/1‑252 (MQ=255)
                                                                                                                               cgTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCt                                                                                                                                                          <  2:875484/266‑1 (MQ=255)
                                                                                                                                gTCAGGGGTTCAGGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGAAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACAGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTACTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   <  1:1828229/288‑1 (MQ=255)
                                                                                                                                gTCAGGGCGTCACGGGCGGATGCCACCTTCGGTTGGCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGGGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGGTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGGGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGGCGGTGGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   <  1:618497/288‑1 (MQ=255)
                                                                                                                                gTCAGGGCGTCACGGACGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   <  1:984293/288‑1 (MQ=255)
                                                                                                                                         tCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   >  2:1278054/1‑279 (MQ=255)
                                                                                                                                          cACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaac                                                                                                                        >  1:1232367/1‑289 (MQ=255)
                                                                                                                                               gCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAAcgc                                                                                                                   >  1:2393422/1‑289 (MQ=255)
                                                                                                                                                     tCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTaaa                                                                                                             <  2:1232367/289‑1 (MQ=255)
                                                                                                                                                     tCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGGGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTaaa                                                                                                             <  2:3121188/289‑1 (MQ=255)
                                                                                                                                                       cACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGcc                                                                                                                                                                               >  1:2771064/1‑221 (MQ=255)
                                                                                                                                                       cACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGcc                                                                                                                                                                               <  2:2771064/221‑1 (MQ=255)
                                                                                                                                                         ccTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGt                                                                                                         <  1:1863537/289‑1 (MQ=255)
                                                                                                                                                            tCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGca                                                                                                                                                                                                                                                                           <  1:2081241/125‑1 (MQ=255)
                                                                                                                                                            tCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGca                                                                                                                                                                                                                                                                           >  2:2081241/1‑125 (MQ=255)
                                                                                                                                                             cGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   >  1:549216/1‑259 (MQ=255)
                                                                                                                                                               gTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAgc                                                                                                                                                                      <  2:2316172/222‑1 (MQ=255)
                                                                                                                                                               gTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAgc                                                                                                                                                                      >  1:2316172/1‑222 (MQ=255)
                                                                                                                                                                     cGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCAcc                                                                                             <  2:572254/289‑1 (MQ=255)
                                                                                                                                                                              cATCACTGTGCAGTGTCTGGCGTTGCAGGTTCGAGATCGAAACCGTGTGGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGcc                                                                                     <  1:876618/288‑1 (MQ=255)
                                                                                                                                                                                  aCTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGACTCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTTCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTCAACCGGTTTTACTTCCAACCATTACCCCACt                                                                                 >  2:258593/1‑288 (MQ=255)
                                                                                                                                                                                  aCTGTGCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACtt                                                                                <  2:1451581/289‑1 (MQ=255)
                                                                                                                                                                                    tgtgCAGTGTCTGGCGTTGCAGATTGGAGGGCGAAACCGTGTCGAAGTCGGGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTcc                                                                                                <  2:3288240/271‑1 (MQ=255)
                                                                                                                                                                                    tgtgCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGAt                                                                                                                                                                                    <  2:3191548/187‑1 (MQ=255)
                                                                                                                                                                                      tgCAGTGTCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCg                                                                                       >  1:1052283/1‑278 (MQ=255)
                                                                                                                                                                                          gtgtCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCa                                                                                                                                                                                                                                                           >  1:336611/1‑111 (MQ=255)
                                                                                                                                                                                           tgtCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   >  2:1047216/1‑229 (MQ=255)
                                                                                                                                                                                             tCTGGCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaaca                                                                                                                       <  2:1945192/239‑1 (MQ=255)
                                                                                                                                                                                                ggCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGcc                                                                                     >  1:1398378/1‑270 (MQ=255)
                                                                                                                                                                                                ggCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGcc                                                                                     <  2:1398378/270‑1 (MQ=255)
                                                                                                                                                                                                ggCGTTGCAGATTCGAGAGCGAAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGa                                                                  >  2:1720992/1‑289 (MQ=255)
                                                                                                                                                                                                           ttcgagagcgaaaCCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTc                                                          <  1:1720992/275‑1 (MQ=255)
                                                                                                                                                                                                               agagcgaaaCCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACg                                                   >  1:936712/8‑289 (MQ=255)
                                                                                                                                                                                                                           tgtCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGtt                                        >  1:3138190/1‑288 (MQ=255)
                                                                                                                                                                                                                           tgtCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGC‑TGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGAttt                                                                                                                                                                                  <  1:2596491/149‑1 (MQ=255)
                                                                                                                                                                                                                              cGAAGTCGAGCCGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGcc                                     <  2:642228/288‑1 (MQ=255)
                                                                                                                                                                                                                              cGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGcc                                     <  1:2041226/288‑1 (MQ=255)
                                                                                                                                                                                                                                     gAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGa                                                                                                                                                        <  2:669576/166‑1 (MQ=255)
                                                                                                                                                                                                                                     gAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGa                                                                                                                                                        >  1:669576/1‑166 (MQ=255)
                                                                                                                                                                                                                                      agcagcGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCCATCGCGTTCCAGCCCGATACACGAGTTGCCGTGGCCaaa                            >  2:1343040/1‑289 (MQ=255)
                                                                                                                                                                                                                                        cagcGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg                           <  1:424564/288‑1 (MQ=255)
                                                                                                                                                                                                                                         agcGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAg                           <  1:635751/287‑1 (MQ=255)
                                                                                                                                                                                                                                           gccagggTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAg                        <  1:1625948/283‑1 (MQ=255)
                                                                                                                                                                                                                                                  ttACCGACCACGGCGCTTGCCAGATGCGGGAGGGCTGAACAGCCGAGGCCGCCAAGGCCCACAAGCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCGCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata                 <  2:459377/288‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCAt                                                                                          >  1:2207390/1‑212 (MQ=255)
                                                                                                                                                                                                                                                     ccGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCAt                                                                                          <  2:2207390/212‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATg             >  1:3267338/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      cGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATgt            <  1:1343040/289‑2 (MQ=255)
                                                                                                                                                                                                                                                        acacCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAgcgc                                                                                                                                                                    <  1:2950986/135‑1 (MQ=255)
                                                                                                                                                                                                                                                          acCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata                 <  2:2155277/280‑1 (MQ=255)
                                                                                                                                                                                                                                                               cgcTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                                                                   >  1:160704/1‑161 (MQ=255)
                                                                                                                                                                                                                                                                gcTTGCCAGAGCCTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCAACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt  <  2:716853/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGcc                                                                                     <  1:3205931/202‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |||                                                                                                                                                                                                                                                                                
GTTGATTACGTACCGCAACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCATCACTGTGCAGTGTCTGGCGTTGCAGATTGCTGAGGCTAACCGTGTCGAAGTCGAGCAGCGTCAGGTTACCGACACCGGCGCTTGCCAGATACTGGCTGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGC‑AGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGACTACCCTGAT  >  CP042583/727612‑728156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A