Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F210 I1 R1	4928	385.2	6658600	97.5%	6492135	231.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	764,707	3 bp→CGA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	764,707	0	G	C	99.4%	819.0 / NA	347	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/2); new base C (180/164); total (181/166)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	764,708	0	C	G	99.7%	822.0 / NA	347	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (181/164); total (181/166)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	764,709	0	T	A	99.4%	821.8 / NA	347	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (181/162); total (181/166)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

CCTCCGCTCAAAACGTGTCGCGCGCAGGTCAATGCGTTTGAGAATGTACTGACTGAGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CGCTTTTGCTCAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGGCTGGCATAGGCGGCATACAGCAGACTCAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCGCTAAGCGTCAGCAGCAGCTGGCTGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCA  >  CP042583/764437‑764984

|||

ccTCCGCTCAAAACGTGTTGCGGGCAGGTCAAGGCGTTTGAGAATGTACTGAATGAGTAGGGTGATCACCAGGTAAAGTGCCGCCGCCACACTGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACCCTAATCCAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAg                                                                                                                                                                                                                                                                      <  2:1992377/288‑1 (MQ=255)

                             cAATGCGTTTGAGAATGTACTGACTGAGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCg                                                                                                                                                                                                                                         <  2:2222163/288‑1 (MQ=255)

                                     ttGAGAATGTACTGACTGAGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCCGCGCCTGACCAGACCACCACTCACCCACCGGCACCGCtt                                                                                                                                                                                                                                >  2:2461310/1‑289 (MQ=255)

                                            tGTACTGACTGAGAAGGGTGGCCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAAcgc                                                                                                                                                                                                                         <  2:557182/289‑1 (MQ=255)

                                                   actgaGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGAc                                                                                                                                                                                                                                                              <  1:1396065/245‑1 (MQ=255)

                                                   actgaGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGa                                                                                                                                                                                                                  >  1:1937277/1‑289 (MQ=255)

                                                      gaGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACt                                                                                                                                                                                                                                                  >  1:1452573/1‑254 (MQ=255)

                                                       aGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAAc                                                                                                                                                                                                                           <  1:2256662/276‑1 (MQ=255)

                                                             gTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGAc                                                                                                                                                                                                                                                              <  1:1437994/235‑1 (MQ=255)

                                                             gTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTtgcg                                                                                                                                                                                                        >  1:720010/1‑287 (MQ=255)

                                                             gTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTtgc                                                                                                                                                                                                         <  1:3141051/288‑2 (MQ=255)

                                                                 tCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTtgcgaggc                                                                                                                                                                                                    <  1:401954/289‑7 (MQ=255)

                                                                   aCCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTtgcgaggcat                                                                                                                                                                                                  <  2:1033895/289‑9 (MQ=255)

                                                                       ggTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAATTCTTTAACCCTAACCAACCTAACAGGCGCGTTTTCTTT‑AGCAAGCACCAGCAACTGGTACCGGAGGCAGGGAAGC‑CATTGCCCCACCACCTCGGGCTCACCAGGCCGAAAAAAGAAAG‑CCGATTCGAACAGCCCCGGCGCCTGCCCAGACCCCCACTACCCCACCGGCCCCCCTTCCACCGCCCCCCGATCCGttggcgacccaaggcc                                                                                                                                                                                              >  2:2790329/1‑275 (MQ=255)

                                                                       ggTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGGGGCATAggc                                                                                                                                                                                              >  1:1975482/1‑289 (MQ=255)

                                                                       ggTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAggc                                                                                                                                                                                              >  1:1095133/1‑289 (MQ=255)

                                                                        gTAAATGGCACCCGCCACAATGGTACAGTTAAAGGGTACCGGGGTACGAGTAGCAATTCTTTTGTTTTGCAGCATTAAATCATTCACCCTAATCAAACTGACCAGGGCGGTAACTTTCAGA‑ACCACCAGCCACTGGTGACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAgg                                                                                                                                                                                               <  2:2925315/287‑1 (MQ=255)

                                                                        gTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTtg                                                                                                                                                                                                          >  1:329256/1‑276 (MQ=255)

                                                                         tAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCCGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAggcg                                                                                                                                                                                             <  2:2403061/288‑1 (MQ=255)

                                                                         tAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAgg                                                                                                                                                                                               <  1:3001247/286‑1 (MQ=255)

                                                                            tatgcggccgccACAATGTAGCAGTGAAATGGTGCCGGGGTACGAGTAGCGATGCTGTTTGTTTGCAGCATTAAATCATTCACACTAGTCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCAt                                                                                                                                                                                         <  2:14528/283‑1 (MQ=255)

                                                                              cgccgccgcCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGcc                                                                                                                                                                                                                                                                  <  1:79997/214‑1 (MQ=255)

                                                                                  gccgccACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCa                                                                                                                                                                                                                                      >  2:2265661/1‑238 (MQ=255)

                                                                                       cacaATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATAcagcagtgac                                                                                                                                                                              <  1:2055782/289‑5 (MQ=255)

                                                                                                ccAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGAt                                                                                                                                                                      <  1:2959637/288‑1 (MQ=255)

                                                                                                   ggTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CTGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGac                                                                                                                                                                   >  2:412313/1‑288 (MQ=255)

                                                                                                      aaaTGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGAcacc                                                                                                                                                                >  1:3191646/1‑288 (MQ=255)

                                                                                                        aTGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGa                                                                                                                                                                                                                                                          >  2:2984006/1‑196 (MQ=255)

                                                                                                        aTGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACcaca                                                                                                                                                             >  1:911979/1‑289 (MQ=255)

                                                                                                        aTGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACcaca                                                                                                                                                             >  1:1988547/1‑289 (MQ=255)

                                                                                                             tCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGa                                                                                                                                                        >  1:1339555/1‑289 (MQ=255)

                                                                                                              ccTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGaa                                                                                                                                                       <  1:667575/289‑1 (MQ=255)

                                                                                                              ccTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGaa                                                                                                                                                       <  2:957863/289‑1 (MQ=255)

                                                                                                              ccTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGa                                                                                                                                                        >  1:472223/1‑288 (MQ=255)

                                                                                                              catGGGTACGAGTGGCGATGTGTGTGGTTTCGAGTATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACCGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGaa                                                                                                                                                       <  2:2577687/287‑1 (MQ=255)

                                                                                                                 gggTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACgg                                                                                                                                                    >  1:3264046/1‑289 (MQ=255)

                                                                                                                 gggTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACgg                                                                                                                                                    >  1:2575279/1‑289 (MQ=255)

                                                                                                                   gTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGa                                                                                                                                                        >  1:2490780/1‑283 (MQ=255)

                                                                                                                      cGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCTGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGCAACGGGCTa                                                                                                                                                >  2:1827154/1‑288 (MQ=255)

                                                                                                                        aGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCAt                                                                                                                                                                                         >  1:1737695/1‑245 (MQ=255)

                                                                                                                        aGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCAt                                                                                                                                                                                         <  2:1737695/245‑1 (MQ=255)

                                                                                                                        aGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGATACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAggcg                                                                                                                                                                                             <  2:1216587/241‑1 (MQ=255)

                                                                                                                              gATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCCGAAAGGAACGGGCCAACGtgtaag                                                                                                                                       >  2:881025/1‑284 (MQ=255)

                                                                                                                                   tttttGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTtct                                                                                                                                   <  1:2002917/288‑1 (MQ=255)

                                                                                                                                        gtttgCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGt                                                                                                                             <  2:1095133/289‑1 (MQ=255)

                                                                                                                                          ttgCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTcc                                                                                                                           <  1:978760/289‑1 (MQ=255)

                                                                                                                                           tgCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGGTCTCAATGTCCa                                                                                                                          >  1:879265/1‑289 (MQ=255)

                                                                                                                                            gcagcaTTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCAcaacgg                                                                                                                                                                                                                                              <  2:1950402/172‑6 (MQ=255)

                                                                                                                                            gcagcaTTAAAGCATTCACACGAATCAAACTGCCCAGCGCGGTAGCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCAt                                                                                                                         <  2:139650/289‑1 (MQ=255)

                                                                                                                                             cagcaTTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCAt                                                                                                                                                                                         >  1:945435/1‑224 (MQ=255)

                                                                                                                                              agcaTTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTCCGAGGTATAGGCGGCATACAGCAGCGCCAGAGCGATGCCACCACAAAGAGACAGGCTACCGTCGAAGTATCCAATGtgccac                                                                                                                        >  2:2122926/1‑283 (MQ=255)

                                                                                                                                                          ttCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGAccc                                                                                                                                                                                                                                             <  2:1258777/159‑1 (MQ=255)

                                                                                                                                                          ttCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGa                                                                                                                                                                    >  2:712320/1‑232 (MQ=255)

                                                                                                                                                              cacTAATCAACCTCACCGACGCGGTATCATTCAGA‑GGCACCAGCCACTGGTTCCCAAGGCAAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCAc                                                                                                       <  2:1339555/289‑1 (MQ=255)

                                                                                                                                                               acTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGCCCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGCCAGAGCGAGGACCCCACCAAGGAACGGCCTAACGTCGAACTTCTCAATGTCCATCTGCaattgcatcacacag                                                                                                      >  2:2533707/1‑275 (MQ=255)

                                                                                                                                                               acTAATCAAACTGACCAGCGAGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACg                                                                                                      >  1:358426/1‑289 (MQ=255)

                                                                                                                                                                  aaTCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAAc                                                                                                   <  1:1028758/289‑1 (MQ=255)

                                                                                                                                                                    acgaccgtaCCAGCGCTGTTTTTTTCCGG‑CGCACCAGGCAGACGTTACCGAGGCCCGCCAGAGTATGCGTCCACAGCAGCGGCATAAAAG‑GACAGACACCCATAGCCGATTTCGC‑AAGCCTCTGCGGCTGACCAGACTCCCACTGACCCACCCGCACCGCTTTTACCCCGACCCGAGGCGTTTGCGAGGAATAGGCGGCATACAGCAGTGACAGAGCGCTGACACCGCAAAGGAACGGGCTAACGTCGAAGTTCTCAATCTCCATCTGAACTGCGATATGCACGAAccc                                                                                                 <  2:827266/281‑1 (MQ=255)

                                                                                                                                                                         cTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGa                                                                                                                                                                                                                                                >  2:772192/1‑141 (MQ=255)

                                                                                                                                                                         cTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGAt                                                                                            >  2:3115765/1‑289 (MQ=255)

                                                                                                                                                                           gACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGAc                                                                                                                                                                                                                                                              <  2:1611207/125‑1 (MQ=255)

                                                                                                                                                                           gACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGAc                                                                                                                                                                                                                                                              >  1:1611207/1‑125 (MQ=255)

                                                                                                                                                                           gACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAAc                                                                                                                                                                                                                           >  2:2054462/1‑160 (MQ=255)

                                                                                                                                                                           gACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATAcag                                                                                                                                                                                     <  1:1258449/198‑1 (MQ=255)

                                                                                                                                                                           gACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTg                                                                                          >  1:113551/1‑289 (MQ=255)

                                                                                                                                                                           gACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTg                                                                                          >  1:2680583/1‑289 (MQ=255)

                                                                                                                                                                            aCCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGa                                                                                                                                                                       <  2:503432/211‑1 (MQ=255)

                                                                                                                                                                             ccAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCAt                                                                                                                                                                                         <  1:3064402/192‑1 (MQ=255)

                                                                                                                                                                             ccAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCAt                                                                                                                                                                                         >  2:3064402/1‑192 (MQ=255)

                                                                                                                                                                                 cgcgGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGGTAG‑CCGATTTCGACAGCCCCAGCGCCTGACCa                                                                                                                                                                                                                                                            <  1:2440426/121‑1 (MQ=255)

                                                                                                                                                                                      tATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGa                                                                                                                                                                       <  2:1139449/201‑1 (MQ=255)

                                                                                                                                                                                           ttCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCa                                                                                                                    >  2:3221533/1‑247 (MQ=255)

                                                                                                                                                                                           ttCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATccg                                                                          >  2:2950773/1‑287 (MQ=255)

                                                                                                                                                                                                c‑agcaCCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAAccc                                                                                                 >  1:1836659/1‑261 (MQ=255)

                                                                                                                                                                                                   gcaCCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATAcag                                                                                                                                                                                     <  2:452776/175‑1 (MQ=255)

                                                                                                                                                                                                         gCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGa                                                                                                     >  2:272178/1‑249 (MQ=255)

                                                                                                                                                                                                              tGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCAc                                                                                                       >  1:2097037/1‑242 (MQ=255)

                                                                                                                                                                                                              tGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACg                                                                                                      >  1:570754/1‑243 (MQ=255)

                                                                                                                                                                                                              tGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACg                                                                                                      <  2:570754/243‑1 (MQ=255)

                                                                                                                                                                                                              tGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGaa                                                                                  >  1:2147224/1‑263 (MQ=255)

                                                                                                                                                                                                              tGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTcagcagca                                                           >  1:2325659/1‑286 (MQ=255)

                                                                                                                                                                                                               ggTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAAccc                                                                                                 <  2:2884124/247‑1 (MQ=255)

                                                                                                                                                                                                                   aCCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCAt                                                                                                                         >  1:758902/1‑219 (MQ=255)

                                                                                                                                                                                                                   aCCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAgctgcgag                                                   >  1:2626800/1‑285 (MQ=255)

                                                                                                                                                                                                                        ggCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAg                                                                                                                                       >  2:1657312/1‑200 (MQ=255)

                                                                                                                                                                                                                          ccAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGa                                                                                                                                                                    >  1:2750161/1‑169 (MQ=255)

                                                                                                                                                                                                                          ccAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCaa                                            >  1:1664484/1‑289 (MQ=255)

                                                                                                                                                                                                                                  cgcATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGa                                                                                                                                                                       >  2:750493/1‑158 (MQ=255)

                                                                                                                                                                                                                                  cgcATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCa                                                                                                        >  2:3295462/1‑221 (MQ=255)

                                                                                                                                                                                                                                       gACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAg                               <  1:283889/289‑1 (MQ=255)

                                                                                                                                                                                                                                        aCGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCaa                                                                                                                                <  2:3279681/191‑1 (MQ=255)

                                                                                                                                                                                                                                         cGCCACATCTGCGGCATCACCA‑GACGGATAAAGATAG‑CCGAGTTCGACAGCCCCATCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGca                             <  1:2533707/289‑1 (MQ=255)

                                                                                                                                                                                                                                            cacaTCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAg                                                                                                                                                                                                <  2:1558926/123‑1 (MQ=255)

                                                                                                                                                                                                                                            cacaTCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAg                                                                                                                                                                                                >  1:1558926/1‑123 (MQ=255)

                                                                                                                                                                                                                                            cacaTCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGcacc                           >  1:539789/1‑288 (MQ=255)

                                                                                                                                                                                                                                                            ccA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAg                                                                                 <  1:1225912/218‑1 (MQ=255)

                                                                                                                                                                                                                                                            ccA‑GACGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAg          >  1:2925919/1‑289 (MQ=255)

                                                                                                                                                                                                                                                              caaaCGGAAAAAGATAA‑CCGATTTCGACACGCCCCGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAgg         <  2:2626800/286‑1 (MQ=255)

                                                                                                                                                                                                                                                                  cGGAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCa                                                                                                        <  2:1439155/190‑1 (MQ=255)

                                                                                                                                                                                                                                                                   ggAAAAAGATAG‑CCGATTTCGACAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGCGAGGCATAGGCGGCATACAGCAGTGACAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCCGAAAGCGTCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACg    <  2:924280/289‑1 (MQ=255)

|||

CCTCCGCTCAAAACGTGTCGCGCGCAGGTCAATGCGTTTGAGAATGTACTGACTGAGCAGGGTGATCACCAGGTAAATCGCCGCCGCCACAATGTACCAGGTAAATGGTTCCTGGGTACGAGTAGCGATGCTTTTTGTTTGCAGCATTAAATCATTCACACTAATCAAACTGACCAGCGCGGTATCTTTCAGC‑AGCACCAGCCACTGGTTACCGAGGCCAGGCAGCGCATGACGCCACATCTGCGGCATCACCA‑GACGGAAAAAGATAG‑CGCTTTTGCTCAGCCCCAGCGCCTGACCAGACTCCCACTGACCCACCGGCACCGCTTTCAACGCGCCCCGAAGCGTTTGGCTGGCATAGGCGGCATACAGCAGACTCAGAGCGATGACACCACAAAGGAACGGGCTAACGTCGAAGTTCTCAATGTCCATCTGCACTGGGATCTGCACGAACCCAAGATTGATAGTGAAGCCATCGCTAAGCGTCAGCAGCAGCTGGCTGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCA  >  CP042583/764437‑764984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A