Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 829,269 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583829,2690AT99.5% 868.9 / NA 364intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (184/178);  total (186/178)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
*CP042583829,2700GC99.5% 875.0 / ‑6.0 364intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (184/178);  minor base T (2/0);  total (186/178)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583829,2710CG99.5% 861.7 / NA 364intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (184/178);  total (186/178)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

TGTGCGTGGCGAAGCGTGGCAATGGCAAGGATTGCGTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACCAGTAGTAGC‑TTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCAAGCGCAAGC‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTAGTGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCA  >  CP042583/828983‑829546
                                                                                                                                                                                                                                                                                                |||                                                                                                                                                                                                                                                                                    
                       ggcaaGGATTGCGTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCg                                                                                                                                                                                                                                                              >  1:496675/1‑289 (MQ=255)
                        gcaaGGATTGCGTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCg                                                                                                                                                                                                                                                              >  1:2331479/1‑288 (MQ=255)
                                tGCGTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGcc                                                                                                                                                                                                                                                        <  2:3041116/286‑1 (MQ=255)
                                   gTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTaa                                                                                                                                                                                                                                                  <  1:145927/289‑1 (MQ=255)
                                   gTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTaa                                                                                                                                                                                                                                                  <  1:275093/289‑1 (MQ=255)
                                   gTTTCAGCGCGAACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTaa                                                                                                                                                                                                                                                  <  1:13763/289‑1 (MQ=255)
                                     ttCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACa                                                                                                                                                                                                                                                <  2:1826799/289‑1 (MQ=255)
                                       cAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGTGGGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACa                                                                                                                                                                                                                                                >  1:2971841/1‑288 (MQ=255)
                                       cAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGTGGGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACa                                                                                                                                                                                                                                                <  2:2971841/288‑1 (MQ=255)
                                       cAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACaaa                                                                                                                                                                                                                                              >  1:294276/1‑289 (MQ=255)
                                               aCTGGCCTTTACAAGGTATCAAAGATAAAGGAAATAACCATGCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCCTTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      <  2:1682346/289‑1 (MQ=255)
                                               aCTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      <  1:1619756/289‑1 (MQ=255)
                                               aCTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      >  1:955028/1‑289 (MQ=255)
                                                   gCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGa                                                                                                                                                                                                                                                             <  2:2253484/262‑1 (MQ=255)
                                                         aCAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCaa                                                                                                                                                                                                                            <  1:2271533/289‑1 (MQ=255)
                                                             ggTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGc                                                                                                                                                                                                                        >  2:2503732/1‑289 (MQ=255)
                                                               tAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCa                                                                                                                                                                                                                       <  1:139087/288‑1 (MQ=255)
                                                               tAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCa                                                                                                                                                                                                                       >  2:139087/1‑288 (MQ=255)
                                                                  cAAAGATAAAGGAGATAACCATTCCTGTGTTGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGCAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGACAGCAAGTGCAGGCAACATAGATTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCGATTAGTTCCGCGACTGAAGGTCAAAGC‑TGCATGAGC‑ATCGCCATCGCACTACAACGCATGGCGTCTGACCTCTATCAAACTTAAACATCGCCATCAACCGCataga                                                                                                                                                                                                                   >  2:186827/1‑285 (MQ=255)
                                                                   aaCGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGa                                                                                                                                                                                                                   <  1:2777997/288‑1 (MQ=255)
                                                                       aTAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGAc                                                                                                                                                                                                                                                            <  2:2723069/243‑1 (MQ=255)
                                                                       aTAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      >  1:2475859/1‑265 (MQ=255)
                                                                       aTAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCa                                                                                                                                                                                                              >  1:1007149/1‑289 (MQ=255)
                                                                       aTAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCa                                                                                                                                                                                                              >  1:668370/1‑289 (MQ=255)
                                                                              aaaTAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGAt                                                                                                                                                                                                        <  2:836404/288‑1 (MQ=255)
                                                                                aTAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGa                                                                                                                                                                                                     <  1:1930092/289‑1 (MQ=255)
                                                                                aTAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGa                                                                                                                                                                                                     <  1:1133492/289‑1 (MQ=255)
                                                                                       ttCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACa                                                                                                                                                                                                                                                >  1:177883/1‑239 (MQ=255)
                                                                                          cTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACAt                                                                                                                                                                                           <  2:1933812/289‑1 (MQ=255)
                                                                                          cTGGGTGGATAAGGTTGATGCACGGACCATTCGCATGCGTCTAACCGGTGAGATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACAt                                                                                                                                                                                           <  2:1342930/289‑1 (MQ=255)
                                                                                           tgtgtgGGTAAGGTTTATGACCGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATc                                                                                                                                                                                          <  1:1348606/289‑1 (MQ=255)
                                                                                            gtgtgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCa                                                                                                                                                                                         <  2:1007149/289‑1 (MQ=255)
                                                                                             tgtgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCaa                                                                                                                                                                                                             >  1:2642627/1‑268 (MQ=255)
                                                                                             tgtgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCaa                                                                                                                                                                                        >  2:535465/1‑289 (MQ=255)
                                                                                             tgtgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCaa                                                                                                                                                                                        >  1:43712/1‑289 (MQ=255)
                                                                                              gtgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTc                                                                                                                                                                                                >  2:928140/1‑280 (MQ=255)
                                                                                               tgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCaa                                                                                                                                                                                                             >  2:2863617/1‑266 (MQ=255)
                                                                                               tgGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCCACCAACTGCAACGATAACAATGGATTGATACCCCACAACAAgg                                                                                                                                                                                      >  2:784921/1‑289 (MQ=255)
                                                                                                       gTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGtt                                                                                                                                                                                                                                           <  2:889526/228‑1 (MQ=255)
                                                                                                       gTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGtt                                                                                                                                                                                                                                           >  1:889526/1‑228 (MQ=255)
                                                                                                         tgatgaCGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCaa                                                                                                                                                                                                             >  2:1585345/1‑256 (MQ=255)
                                                                                                                 ggACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAAttt                                                                                                                                                                    >  1:3088545/1‑289 (MQ=255)
                                                                                                                  gACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAAtttt                                                                                                                                                                   >  1:3028819/1‑289 (MQ=255)
                                                                                                                     gAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAAttttt                                                                                                                                                                  <  2:257207/287‑2 (MQ=255)
                                                                                                                            aTTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTg                                                                                                                                                                                                                                                          >  1:1272584/1‑192 (MQ=255)
                                                                                                                             ttcttcTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      >  1:2609816/1‑211 (MQ=255)
                                                                                                                             ttcttcTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAg                                                                                                                                                         >  2:210365/1‑288 (MQ=255)
                                                                                                                                ttctAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAg                                                                                                                                                                                                                                        <  1:2852834/206‑1 (MQ=255)
                                                                                                                                ttctAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGa                                                                                                                                                                                                                   <  1:1522720/227‑1 (MQ=255)
                                                                                                                                     aCCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAGGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGAt                                                                                                                                                                                                        <  2:660995/233‑1 (MQ=255)
                                                                                                                                     aCCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATc                                                                                                                                                                                                                              <  2:3174052/211‑1 (MQ=255)
                                                                                                                                     aCCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCa                                                                                                                                                                                         >  2:3089108/1‑248 (MQ=255)
                                                                                                                                          tGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCa                                                                                                                                                                                                                       >  2:1451284/1‑213 (MQ=255)
                                                                                                                                          tGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTagc                                                                                                                                           >  1:784224/1‑289 (MQ=255)
                                                                                                                                          tGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTagc                                                                                                                                           >  2:2820703/1‑289 (MQ=255)
                                                                                                                                          tGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTag                                                                                                                                            <  2:671749/288‑1 (MQ=255)
                                                                                                                                                  aaGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCaa                                                                                                                                                                                                             >  2:1484974/1‑215 (MQ=255)
                                                                                                                                                  aaGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAg                                                                                                                                   >  2:330712/1‑289 (MQ=255)
                                                                                                                                                    gCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAAttttt                                                                                                                                                                  <  1:2086309/256‑2 (MQ=255)
                                                                                                                                                       ccAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACAt                                                                                                                                                                                           <  1:2179122/228‑1 (MQ=255)
                                                                                                                                                       ccAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCa                                                                                                                                                                                         >  1:569652/1‑230 (MQ=255)
                                                                                                                                                         aGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      <  1:1051571/183‑1 (MQ=255)
                                                                                                                                                         aGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCaa                                                                                                                            >  1:2130109/1‑289 (MQ=255)
                                                                                                                                                                cAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTAc                                                                                                                     <  2:1253100/289‑1 (MQ=255)
                                                                                                                                                                     gATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGt                                                                                                                >  1:630948/1‑289 (MQ=255)
                                                                                                                                                                        gCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCa                                                                                                                                                                                                                                      >  1:2895768/1‑168 (MQ=255)
                                                                                                                                                                        gCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCt                                                                                                                                                                                                                                 >  1:2308479/1‑173 (MQ=255)
                                                                                                                                                                        gCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCt                                                                                                                                                                                                                                 <  2:2308479/173‑1 (MQ=255)
                                                                                                                                                                             gCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTatca                                                                                                        >  1:196919/1‑289 (MQ=255)
                                                                                                                                                                               cGTTACTGGCAGACAGTGAAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTatcat                                                                                                       <  2:2891048/288‑1 (MQ=255)
                                                                                                                                                                                         agcaAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCa                                                                                                                                                                                                                       >  1:203770/1‑166 (MQ=255)
                                                                                                                                                                                                gCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCa                                                                                     <  1:2535710/289‑1 (MQ=255)
                                                                                                                                                                                                   ggCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATCTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCCTCAGTGGTTTGGCGTGCCAGTg                                                                                  >  2:2066840/1‑289 (MQ=255)
                                                                                                                                                                                                       acatgtcTTCAGTTACCTAAACATGGCAGTAAAACC‑TCACCATCGTTGCAATTAATTCAGCGGGGGAATGGAAAAGG‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATa                                                                              <  2:1958400/283‑1 (MQ=255)
                                                                                                                                                                                                       aCATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATa                                                                              <  1:330712/289‑1 (MQ=255)
                                                                                                                                                                                                        cATTGCTTCAGGTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGc                                                                                       >  2:1139023/1‑279 (MQ=255)
                                                                                                                                                                                                                  ggTACCTCACCATGGCAGTAATACC‑TCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCCGGGGATAACGGGTAGAat                                                                   >  2:479471/1‑289 (MQ=255)
                                                                                                                                                                                                                                           ctcatcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTAc                                                                                                                     <  2:232634/205‑1 (MQ=255)
                                                                                                                                                                                                                                            tcatcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTgg                                          >  2:2946197/10‑289 (MQ=255)
                                                                                                                                                                                                                                            tcatcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTAAATTTTTCAGCGCAAGGCTGGCGGTTTCGCAGCCTCAGGGAGCAAACTTTCCCATGTTGGTATCATCAGTGGTTTGGCGTGTCAGTGGATAACGGGTAGAATATGCGGCTCTTTCAACAAAGTCTgg                                          >  2:1750573/10‑289 (MQ=255)
                                                                                                                                                                                                                                             catcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCa                                                                                     <  1:327246/237‑1 (MQ=255)
                                                                                                                                                                                                                                             catcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCa                                                                                     >  2:327246/9‑245 (MQ=255)
                                                                                                                                                                                                                                              atcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACggg                                                                         <  2:507610/249‑1 (MQ=255)
                                                                                                                                                                                                                                               tcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCTGCCTCAGGGAGCAAATTTTACCTCg                                                                                                                 <  2:108522/209‑1 (MQ=255)
                                                                                                                                                                                                                                               tcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTatcat                                                                                                       >  2:581314/7‑225 (MQ=255)
                                                                                                                                                                                                                                               tcatcgttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGCTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGttt                                       >  1:2363392/7‑289 (MQ=255)
                                                                                                                                                                                                                                                     ttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCg                                                                                                                 <  2:2167289/209‑1 (MQ=255)
                                                                                                                                                                                                                                                     ttGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCCGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                 >  2:359760/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                 <  1:3147967/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            tAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCT‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATaa                          <  1:2884050/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            tAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATGACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATaa                          >  2:1154330/1‑289 (MQ=255)
                                                                                                                                                                                                                                                             aaTTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCg                                                                                                                                                  >  1:908700/1‑168 (MQ=255)
                                                                                                                                                                                                                                                                tCAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGAc                      <  1:1095701/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cAGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                     <  1:1174351/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aGCGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaa                    >  1:473305/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cGAGTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGa                                                                                                                                                                                                     >  1:1144622/1‑110 (MQ=255)
                                                                                                                                                                                                                                                                    agAGTGAATGGAATAGT‑GGAACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                   <  2:242448/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gTGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAt                <  2:2026340/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGAATGGAAAAGT‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAtc               >  2:265671/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    t‑GGCACTCGCATCGGCATCG‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGCTTAGCAGCCTCCGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGTCGTGCCAGTGGATAACGTGTAGAGTATGCGTCTATTGCAACAAAGGCTGGTGTTTTGAATGCATAACGACAAAGAtctcgtctcgtggtc  >  2:2629450/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                                |||                                                                                                                                                                                                                                                                                    
TGTGCGTGGCGAAGCGTGGCAATGGCAAGGATTGCGTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACGATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCCCCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAATACCAGTAGTAGC‑TTGCCATTAATTCAGCGAGTGAATGGAAAAGT‑GGCACTCGCAAGCGCAAGC‑CGCTATAACGCATGGCGACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTAGTGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCA  >  CP042583/828983‑829546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A