Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F210 I1 R1	4928	385.2	6658600	97.5%	6492135	231.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	1,810,277	3 bp→TCG	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	1,810,277	0	A	T	99.7%	884.6 / NA	369	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (184/183); total (185/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,810,278	0	G	C	99.5%	876.0 / NA	369	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/2); new base C (185/182); total (185/184)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,810,279	0	C	G	100.0%	891.3 / NA	369	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (185/184); total (185/184)

Only 100 of 369 total aligned reads displayed.

TGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCAGTTGTTAAATTAATATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTAGCAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTAGCCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCAGCCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTAGCATCATGGGGGAAAATGAGCAGCAATGGGA  >  CP042583/1810002‑1810557

|||

gccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  2:1199544‑M2/85‑1 (MQ=255)

gccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:580567‑M2/85‑1 (MQ=255)

gccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:1900085‑M2/85‑1 (MQ=255)

gccgccggtcagcatgccagactagatgccgttgcggagttaaggccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:3319977‑M2/85‑1 (MQ=255)

  cgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACt                                                                                                                                                                                                                                                                           <  1:491265‑M2/87‑1 (MQ=255)

        tctgcatgccagcctggatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTAt                                                                                                                                                                                                                                                                      <  2:890375‑M2/92‑1 (MQ=255)

                          tgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:1169793‑M2/85‑1 (MQ=255)

                          tgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             >  2:1169793‑M2/179‑263 (MQ=255)

                             ctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACg                                                                                                                                                                                                                                                >  1:1735554‑M2/176‑289 (MQ=255)

                              tttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcGCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGGTTTGCAACGCCTAcgg                                                                                                                                                                                                                                               >  2:1379046‑M2/175‑288 (MQ=255)

                                tgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGac                                                                                                                                                                                                                                              >  1:845716‑M2/173‑288 (MQ=255)

                                       ttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACt                                                                                                                                                                                                                                                                           >  1:3208942‑M2/166‑252 (MQ=255)

                                        tagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTg                                                                                                                                                                                                                                      <  1:1455660‑M2/124‑1 (MQ=255)

                                            gccgcagcgtaacaccatcaaaacccaaaagtcccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                                                                   <  2:2100360‑M2/127‑1 (MQ=255)

                                            gccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                                                                   <  1:3165821‑M2/127‑1 (MQ=255)

                                            gccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                                                                   <  1:2791803‑M2/127‑1 (MQ=255)

                                            gccgcagcgtaacacaatcaaaacacaaaattaccagaaccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                                                                   <  1:1530493‑M2/127‑1 (MQ=255)

                                            gccgcaacgtaacaccatcaaaacacaaaactaccagacccgcgaccagcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                                                                   <  1:3070113‑M2/127‑1 (MQ=255)

                                               gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             >  2:2768042‑M2/158‑242 (MQ=255)

                                               gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  2:260272‑M2/85‑1 (MQ=255)

                                               gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCa                                                                                                                                                                                                                                                         >  1:1643326‑M2/158‑262 (MQ=255)

                                               gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCggtg                                                                                                                                                                                                                              >  1:1795488‑M2/158‑289 (MQ=255)

                                               gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCggtg                                                                                                                                                                                                                              >  1:622906‑M2/158‑289 (MQ=255)

                                                acgcgtaaccccatcaacacacaaaattaccagccccgggaccctcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtgttctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttatatggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCggtgt                                                                                                                                                                                                                             <  2:1335952‑M2/133‑1 (MQ=255)

                                                 agcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGTTGTGTGCCAAGCCCTACGACAGAGGTTGGgggggttgt                                                                                                                                                                                                                            >  2:653388‑M2/156‑286 (MQ=255)

                                                 agcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCa                                                                                                                                                                                                                                                         <  2:2722291‑M2/105‑1 (MQ=255)

                                                 agcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCggtgtg                                                                                                                                                                                                                            <  1:1514274‑M2/134‑1 (MQ=255)

                                                    gtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGTGCggtgtg                                                                                                                                                                                                                            <  1:2616850‑M2/134‑1 (MQ=255)

                                                             tcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                                                                   <  1:1179066‑M2/127‑1 (MQ=255)

                                                                        aattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgccaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAGCCTACGATCCAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCttttt                                                                                                                                                                                                     >  2:2390490‑M2/133‑289 (MQ=255)

                                                                                gacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCa                                                                                                                                                                                             >  1:1346597‑M2/125‑289 (MQ=255)

                                                                                gacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTACTAGATGTGTGCCAAAGCCTACGAAACCGGTTGGGTCGGTGTGGTGTTTAAAAGGATAGGCTTTTTTATCTCCa                                                                                                                                                                                             >  2:2798340‑M2/125‑289 (MQ=255)

                                                                                             caagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACgaagtctcgcc                                                                                                                                                                                >  1:1877082‑M2/112‑284 (MQ=255)

                                                                                                          ctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:2435112‑M2/85‑1 (MQ=255)

                                                                                                                 tattgttgagggtgacaaaaccgtggtctatgccgtgcaaaccgggaacgaagccgccgaggcgattcatcactatttagcgggagcttgcTCATGTTACCGAAAGATCTTTCGAT‑TACTTTTTGTGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGa                                                                                                                                                            <  1:2300245‑M2/198‑1 (MQ=255)

                                                                                                                    tgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACt                                                                                                                                                                                                                                                                           >  2:1054382‑M2/89‑175 (MQ=255)

                                                                                                                    tgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACt                                                                                                                                                                                                                                                                           <  1:1054382‑M2/87‑1 (MQ=255)

                                                                                                                         agggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TAATTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGaca                                                                                                                                                                                                                                             >  2:543616‑M2/84‑200 (MQ=255)

                                                                                                                           ggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             >  1:3303265‑M2/82‑166 (MQ=255)

                                                                                                                           ggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGGTCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATa                                                                                                                                                   >  1:645071‑M2/82‑288 (MQ=255)

                                                                                                                           ggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATa                                                                                                                                                   >  1:2328372‑M2/82‑288 (MQ=255)

                                                                                                                             tgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCg                                                                                                                                                >  2:42464‑M2/80‑289 (MQ=255)

                                                                                                                               acaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATa                                                                                                                                                   >  2:962702‑M2/78‑284 (MQ=255)

                                                                                                                                caaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGaca                                                                                                                                                                                                                                             <  1:679108‑M2/117‑1 (MQ=255)

                                                                                                                                caaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGtt                                                                                                                                             >  1:1381606‑M2/77‑289 (MQ=255)

                                                                                                                                     ccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATc                                                                                                                                        >  2:2841871‑M2/72‑289 (MQ=255)

                                                                                                                                       gtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCg                                                                                                                                       >  2:2158599‑M2/70‑288 (MQ=255)

                                                                                                                                        tggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCg                                                                                                                                       >  1:473868‑M2/69‑287 (MQ=255)

                                                                                                                                             tatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCaa                                                                                                                                <  1:2763861‑M2/226‑1 (MQ=255)

                                                                                                                                                  cgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAata                                                                                                                           >  1:3186734‑M2/59‑289 (MQ=255)

                                                                                                                                                    tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATc                                                                                                                                                                                                             <  2:2262134‑M2/149‑1 (MQ=255)

                                                                                                                                                    tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATc                                                                                                                                                                                                             >  1:2262134‑M2/57‑205 (MQ=255)

                                                                                                                                                    tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAagtctcg                                                                                                                                                                                  >  1:1116259‑M2/57‑229 (MQ=255)

                                                                                                                                                    tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAagtctcg                                                                                                                                                                                  <  2:1116259‑M2/176‑4 (MQ=255)

                                                                                                                                                    tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATg                                                                                                                         >  1:730730‑M2/57‑289 (MQ=255)

                                                                                                                                                    tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTGCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGAGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATTGGGGGACAGAAGTTAACGGTTTTATCGgcttcaaaactttg                                                                                                                         >  2:3297689‑M2/57‑284 (MQ=255)

                                                                                                                                                         accgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCt                                                                                                                                                                 >  2:1453145‑M2/52‑244 (MQ=255)

                                                                                                                                                         accgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCa                                                                                                                    >  1:209264‑M2/52‑289 (MQ=255)

                                                                                                                                                            gggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGAt                                                                                                                 <  2:356846‑M2/241‑1 (MQ=255)

                                                                                                                                                              gaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTg                                                                                                               >  2:2051491‑M2/47‑289 (MQ=255)

                                                                                                                                                                                         ctatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTgg                                                                                    <  2:2944974‑M2/270‑1 (MQ=255)

                                                                                                                                                                                          tatttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCGCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGaaa                                                                                                                                                          <  1:2115024‑M2/200‑1 (MQ=255)

                                                                                                                                                                                            tttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             >  2:2545189‑M2/17‑101 (MQ=255)

                                                                                                                                                                                            tttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:2545189‑M2/85‑1 (MQ=255)

                                                                                                                                                                                            tttagagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGgcc                                                                                  >  1:2225555‑M2/17‑288 (MQ=255)

                                                                                                                                                                                               agagggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                                                                             <  1:964359‑M2/85‑1 (MQ=255)

                                                                                                                                                                                                 agggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCt                                                                             >  2:2320173‑M2/12‑288 (MQ=255)

                                                                                                                                                                                                 agggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCt                                                                             >  2:1547566‑M2/12‑288 (MQ=255)

                                                                                                                                                                                                 agggagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTg                                                                            >  1:2981167‑M2/12‑289 (MQ=255)

                                                                                                                                                                                                    gagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCTAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCg                                                                          >  2:402450‑M2/9‑288 (MQ=255)

                                                                                                                                                                                                    gagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACgaagtctcgcc                                                                                                                                                                                >  2:515392‑M2/9‑181 (MQ=255)

                                                                                                                                                                                                    gagcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGatcat                                                                                                                                                                   >  1:1930966‑M2/9‑199 (MQ=255)

                                                                                                                                                                                                     ggcttgcTCATGTTAACGAAAGATCTT‑GCGTGTACTTTTTGGGGCGTGAAGTTTCCCAACCCGTTCTGCCTCGCTTCTTCGCCGGTAGGCAACTGCTATGAGATGGGTGCCAAAGCCTACGAAACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGt                                                                         <  2:3144026‑M2/281‑1 (MQ=255)

                                                                                                                                                                                                      gcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGATGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCg                                                                       >  1:2919693‑M2/7‑289 (MQ=255)

                                                                                                                                                                                                      gcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCaaa                                                                                                                                                                                                                                                       <  2:1502532‑M2/107‑1 (MQ=255)

                                                                                                                                                                                                      gcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAgg                                                                                                                                                                                                                                         >  1:422942‑M2/7‑127 (MQ=255)

                                                                                                                                                                                                      gcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTgg                                                                                    >  1:2176429‑M2/7‑276 (MQ=255)

                                                                                                                                                                                                      gcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCg                                                                       >  1:2506499‑M2/7‑289 (MQ=255)

                                                                                                                                                                                                      gcttgcTCATGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCg                                                                       >  2:3258905‑M2/7‑289 (MQ=255)

                                                                                                                                                                                                        ttgaTCATGTTAACTACAGTTCCTTCGAT‑GTCTTTTTGCGGCGTTAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACTACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGc                                                                     <  2:1877082‑M2/285‑1 (MQ=255)

                                                                                                                                                                                                               tGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCa                                                                                                                                                                                                                                                         <  1:398686/102‑1 (MQ=255)

                                                                                                                                                                                                               tGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATa                                                                                                                                                   <  2:1707572/204‑1 (MQ=255)

                                                                                                                                                                                                               tGTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGGCGGCTgaagga                                                              >  2:1755698/1‑289 (MQ=255)

                                                                                                                                                                                                                gTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAGACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTgaaggaa                                                             >  2:709917/1‑289 (MQ=255)

                                                                                                                                                                                                                gTTAACGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGt                                                                         <  2:3187689/277‑1 (MQ=255)

                                                                                                                                                                                                                    aCGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACg                                                                                                                                                                                          >  1:958883/1‑160 (MQ=255)

                                                                                                                                                                                                                    aCGAAAGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGaa                                                                                              >  1:1262283/1‑252 (MQ=255)

                                                                                                                                                                                                                        aaGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTAccc                                                     <  1:2841871/289‑1 (MQ=255)

                                                                                                                                                                                                                         aGATCTTTCGAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCg                                                    >  1:517408/1‑289 (MQ=255)

                                                                                                                                                                                                                            tctttcgaT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGa                                                  <  2:473868/281‑1 (MQ=255)

                                                                                                                                                                                                                             ctttcgaT‑TACTTTTTGCGGCGTGATGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACa                                                <  2:2919693/283‑1 (MQ=255)

                                                                                                                                                                                                                                      tACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCACTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCGACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTg                                        <  1:2390490/289‑1 (MQ=255)

                                                                                                                                                                                                                                                  cgTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAagag                                                                                           >  1:1598751/1‑226 (MQ=255)

                                                                                                                                                                                                                                                   gTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGAt                                                                                                                 >  1:1324567/1‑203 (MQ=255)

                                                                                                                                                                                                                                                                 cccGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGag             <  2:622906/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                 cccGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAACTATTGATCGCTCTGATCATGGGGGAAAATGa              >  2:1272295/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                     ttCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCt                                                                                                             <  2:2448946/189‑1 (MQ=255)

                                                                                                                                                                                                                                                                      tCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTa                                           <  1:995328/254‑1 (MQ=255)

                                                                                                                                                                                                                                                                      tCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAgcagca        >  1:2672292/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                            tctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCGGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGgga  >  1:1028719/1‑289 (MQ=255)

|||

TGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCAGTTGTTAAATTAATATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTAGCAT‑TACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTAGCCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCAGCCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTAGCATCATGGGGGAAAATGAGCAGCAATGGGA  >  CP042583/1810002‑1810557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A