Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,977,657 6 bp→TGATGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,977,6570AT99.7% 736.7 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (161/144);  minor base G (0/1);  total (161/145)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,977,6580CG100.0% 735.7 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (161/145);  total (161/145)
*CP0425831,977,6590TA99.7% 732.8 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (160/145);  minor base C (1/0);  total (161/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,977,6600AT99.7% 734.5 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (160/144);  minor base C (1/0);  total (161/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,977,6610CG99.7% 726.2 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (160/144);  minor base A (1/0);  total (161/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,977,6620TA99.3% 725.9 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (160/143);  minor base C (1/1);  total (161/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

TGTATTCCGCTGCGCAGTACCAGTGTGTTGGGCTGGTACGCAGGTTCAATGCCTTAAACGCACTAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACGCTACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCACTAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAAACTACTGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATA  >  CP042583/1977392‑1977932
                                                                                                                                                                                                                                                                         ||||||                                                                                                                                                                                                                                                                              
       cGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCACTGAAGATTACTTAACATTAATGCTGAGCAGGCCTCATCAATACAACGCAgg                                                                                                                                                                                                                                                       >  2:1064077/1‑289 (MQ=255)
        gCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGa                                                                                                                                                                                                                                                      >  1:198925/1‑289 (MQ=255)
        gCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGa                                                                                                                                                                                                                                                      >  1:1860100/1‑289 (MQ=255)
          tGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaaa                                                                                                                                                                                                                                                              >  1:2976689/1‑279 (MQ=255)
                 tACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTGAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACCTTATTGATGAGCAGGCATCATCATCAAAACCCAGGATTTGCCTg                                                                                                                                                                                                                                              >  2:467833/1‑288 (MQ=255)
                      gtgtgtTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTa                                                                                                                                                                                                                                         >  1:1312024/1‑288 (MQ=255)
                           ttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGAAAGTTAATTCGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGAAGGATTTTCCTGGCGTAACCaag                                                                                                                                                                                                                                   >  1:124516/8‑289 (MQ=255)
                             ggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc                                                                                                                                                                                                                                                >  2:844144/6‑274 (MQ=255)
                             ggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaagaa                                                                                                                                                                                                                                 >  2:1207956/6‑289 (MQ=255)
                                   gTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTg                                                                                                                                                                                                                                              >  2:17359/1‑270 (MQ=255)
                                            ttCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGACTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGt                                                                                                                                                                                                                   >  2:2924076/1‑288 (MQ=255)
                                             tCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGtt                                                                                                                                                                                                                  <  1:1196869/288‑1 (MQ=255)
                                                    cTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAAc                                                                                                                                                                                                          <  2:185206/289‑1 (MQ=255)
                                                     ttAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACa                                                                                                                                                                                                         >  1:1098428/1‑289 (MQ=255)
                                                             tgaaGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGcc                                                                                                                                                                                                                       >  2:3098332/4‑267 (MQ=255)
                                                             tgaaGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGcc                                                                                                                                                                                                                       <  1:3098332/264‑1 (MQ=255)
                                                              gaaGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc                                                                                                                                                                                                                                                 >  1:843146/3‑240 (MQ=255)
                                                                         ggtggtTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGa                                                                                                                                                                                     >  1:997240/1‑289 (MQ=255)
                                                                         ggtggtTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGa                                                                                                                                                                                     >  1:1502856/1‑289 (MQ=255)
                                                                          gtggtTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAAt                                                                                                                                                                                                                          >  1:245758/1‑251 (MQ=255)
                                                                               tACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGaa                                                                                                                                                                                <  2:2638668/288‑1 (MQ=255)
                                                                                    tCAAATCCACTTCATGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGAATTAACAGGCAAGGTAAAATCATATCGGCAGATTCATCTCAAGCACAGATAGTTAATACGCCCTGAAGATTACTAAACATTAATGACGAGCAGGCCTCATCATTCAAACGCAGGATTTTCCTAGCTTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTACGACCGTGAagagacaaccat                                                                                                                                                                            >  2:2513678/1‑279 (MQ=255)
                                                                                            aCTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATg                                                                                                                                                                                                                         <  2:2403443/234‑1 (MQ=255)
                                                                                            aCTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATg                                                                                                                                                                                                                         >  1:2403443/1‑234 (MQ=255)
                                                                                            aCTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCa                                                                                                                                                                   >  1:630289/1‑288 (MQ=255)
                                                                                                ccTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGGTCTTTTCAAACGAg                                                                                                                                                              >  2:1146041/1‑289 (MQ=255)
                                                                                                  tGGGATTCCAGCCGTTCTGACATATAGGCATTACGTTTAACGCGGAAATCCAGCGCTAATTTCGGATAAACCGAACTCACGAGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAgtt                                                                                                                                                            <  2:1910368/289‑1 (MQ=255)
                                                                                                    ggATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGaa                                                                                                                                                                                >  1:2597886/1‑267 (MQ=255)
                                                                                                        tCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCACTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCa                                                                                                                                                      >  2:3172582/1‑289 (MQ=255)
                                                                                                          cAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAg                                                                                                                                                     >  2:1092547/1‑288 (MQ=255)
                                                                                                              aTTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGAc                                                                                                                                                   <  2:1116323/286‑1 (MQ=255)
                                                                                                                          taGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAg                                                                                                                                                              <  2:1862063/263‑1 (MQ=255)
                                                                                                                               aTTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTc                                                                                                                                                                                                                 >  1:2548367/1‑207 (MQ=255)
                                                                                                                                 tACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcggc                                                                                                                             <  1:1064077/289‑1 (MQ=255)
                                                                                                                                  aCGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGCCGCTTCATTTGCATACTTAcg                                                                                                                               >  2:1244720/1‑286 (MQ=255)
                                                                                                                                  aCGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcggcg                                                                                                                            >  1:494220/1‑289 (MQ=255)
                                                                                                                                    gTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                          <  2:919223/289‑1 (MQ=255)
                                                                                                                                       tAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGa                                                                                                                                                    >  1:84928/1‑260 (MQ=255)
                                                                                                                                       tAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTg                                                                                                                       >  1:101433/1‑289 (MQ=255)
                                                                                                                                             ggACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                          >  2:224826/1‑280 (MQ=255)
                                                                                                                                              gACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACg                                                                                                                 >  1:3148952/1‑288 (MQ=255)
                                                                                                                                              gACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACg                                                                                                                 >  1:218212/1‑288 (MQ=255)
                                                                                                                                               aCATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAg                                                                                                                                                              <  1:2225669/242‑1 (MQ=255)
                                                                                                                                                cATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGaca                                                                                                              <  2:198925/289‑1 (MQ=255)
                                                                                                                                                cATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGaca                                                                                                              <  1:1886053/289‑1 (MQ=255)
                                                                                                                                                 aTCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGacac                                                                                                             <  2:1678403/289‑1 (MQ=255)
                                                                                                                                                           aaTTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCa                                                                                                    >  2:2296691/1‑288 (MQ=255)
                                                                                                                                                               tCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGc                                                                                               <  1:553289/289‑1 (MQ=255)
                                                                                                                                                                 ggATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCg                                                                                              >  1:1417903/1‑288 (MQ=255)
                                                                                                                                                                    taaaccgaaCTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGgcag                                                                                          <  1:771470/281‑1 (MQ=255)
                                                                                                                                                                                  gcgATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAAc                                                                                                                                                                               <  1:2429053/190‑1 (MQ=255)
                                                                                                                                                                                          aCAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGgcaac                                                                    <  2:1980365/289‑1 (MQ=255)
                                                                                                                                                                                              gAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGt                                                                                                                                                                                          <  2:3087505/167‑1 (MQ=255)
                                                                                                                                                                                              gAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGt                                                                                                                                                                                          >  1:3087505/1‑167 (MQ=255)
                                                                                                                                                                                                aaGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCg                                                                                                                                                                                               <  2:352281/160‑1 (MQ=255)
                                                                                                                                                                                                aaGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGGTGCAGCAAAAGTGTTCAGATCGgcaacagcaaa                                                              >  2:445895/1‑288 (MQ=255)
                                                                                                                                                                                                    ttagagctgaTCGGCAGATTCATCTGACGCAACGATAGTGAATACGCCCGGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATGTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGcaacaa                                                            <  2:885948/278‑1 (MQ=255)
                                                                                                                                                                                                    tAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGcaacaa                                                            <  2:2720681/287‑1 (MQ=255)
                                                                                                                                                                                                        aTCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                          >  2:685979/1‑221 (MQ=255)
                                                                                                                                                                                                        aTCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                          <  1:685979/221‑1 (MQ=255)
                                                                                                                                                                                                            tATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTa                                                                                                                   <  1:228221/224‑1 (MQ=255)
                                                                                                                                                                                                             aTCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGaa                                                                                                                                                                                <  1:1685607/162‑1 (MQ=255)
                                                                                                                                                                                                             aTCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCaaaa                                                                                     >  1:733293/1‑253 (MQ=255)
                                                                                                                                                                                                                 gCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAg                                                                                                                                                              <  2:1703990/176‑1 (MQ=255)
                                                                                                                                                                                                                 gCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTc                                                                               <  2:792947/255‑1 (MQ=255)
                                                                                                                                                                                                                 gCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTc                                                                               >  1:792947/1‑255 (MQ=255)
                                                                                                                                                                                                                  cAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAt                                             >  2:634990/1‑288 (MQ=255)
                                                                                                                                                                                                                       tcatctgaaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgaggt                                       <  2:747520/281‑1 (MQ=255)
                                                                                                                                                                                                                        catctgaaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgaggtc                                      <  1:1217924/282‑1 (MQ=255)
                                                                                                                                                                                                                           ctgaaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgaggtcgag                                   >  2:134388/5‑289 (MQ=255)
                                                                                                                                                                                                                                   ccGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGa                                                                                                                      <  1:2792465/198‑1 (MQ=255)
                                                                                                                                                                                                                                   ccGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTa                           >  2:562761/1‑289 (MQ=255)
                                                                                                                                                                                                                                     gATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATc                         <  2:1582417/289‑1 (MQ=255)
                                                                                                                                                                                                                                     gATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATc                         >  1:1808981/1‑289 (MQ=255)
                                                                                                                                                                                                                                      aTAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGAc                                                                                                                                                   >  2:510237/1‑166 (MQ=255)
                                                                                                                                                                                                                                      aTAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCg                        >  1:1933183/1‑289 (MQ=255)
                                                                                                                                                                                                                                      aTAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCg                        >  2:2024188/1‑289 (MQ=255)
                                                                                                                                                                                                                                        aGTTAATACGCCCTGAAGATTCCTGAACATTAATGATGCGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGCCGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGa                      <  2:2680430/289‑1 (MQ=255)
                                                                                                                                                                                                                                            taTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGAt                  <  1:3181631/288‑1 (MQ=255)
                                                                                                                                                                                                                                            aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                          <  2:2358984/185‑1 (MQ=255)
                                                                                                                                                                                                                                            aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                          >  1:2358984/1‑185 (MQ=255)
                                                                                                                                                                                                                                            aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGAt                  >  1:2099962/1‑289 (MQ=255)
                                                                                                                                                                                                                                             aTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTcag                                                  <  1:1624992/256‑1 (MQ=255)
                                                                                                                                                                                                                                             aTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGAtt                 <  2:2099962/289‑1 (MQ=255)
                                                                                                                                                                                                                                              tACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGgcaacagc                                                                 >  2:2440815/1‑240 (MQ=255)
                                                                                                                                                                                                                                               aCGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTg               <  1:2296691/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCa             <  2:1417903/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  cccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCgg                       <  1:2658505/278‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAg                                                                                                                                                                                                        <  1:899651/100‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcggc                                                                                                                             <  2:2586424/175‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcgg                                                                                                                              <  1:421416/174‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCaaaa                                                                                     >  1:1125366/1‑215 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGtt                                                       <  1:2347516/245‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGtt                                                       >  2:2347516/1‑245 (MQ=255)
                                                                                                                                                                                                                                                   ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCa             <  1:2754559/287‑1 (MQ=255)
                                                                                                                                                                                                                                                    cTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAg                                                                             <  1:2140240/222‑1 (MQ=255)
                                                                                                                                                                                                                                                    cTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAg                                                                             >  2:2140240/1‑222 (MQ=255)
                                                                                                                                                                                                                                                        aGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCa      <  1:65165/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        aGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCa      <  2:2213384/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            tACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACt                                                                                                                        <  1:1707826/171‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ||||||                                                                                                                                                                                                                                                                              
TGTATTCCGCTGCGCAGTACCAGTGTGTTGGGCTGGTACGCAGGTTCAATGCCTTAAACGCACTAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACGCTACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCACTAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAAACTACTGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATA  >  CP042583/1977392‑1977932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A