Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 729,232 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583729,2320GC98.6% 335.2 / ‑6.5 146intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base C (60/84);  total (62/84)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583729,2330CG99.3% 333.3 / NA 146intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (61/83);  total (62/84)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583729,2340TA99.3% 339.1 / NA 146intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (61/84);  total (62/84)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 146 total aligned reads displayed.

ACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGGCTAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑GCTCATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGC  >  CP042583/728956‑729512
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
aCCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCg                                                                                                                                                                                                                                                                              <  1:108137/289‑1 (MQ=255)
     tCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCggtgg                                                                                                                                                                                                                                                                          <  2:977778/288‑1 (MQ=255)
      cATAAGACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTa                                                                                                                                                                                                                                                                        <  1:1261327/289‑1 (MQ=255)
         aataCGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAAt                                                                                                                                                                                                                                                                     <  2:1004445/289‑1 (MQ=255)
         aataCGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAAt                                                                                                                                                                                                                                                                     <  1:127207/289‑1 (MQ=255)
           tcggaaggacggTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGGGGCAATCTCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACAGCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGGACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGGGGTGTCGGG‑CGACATCAATCCGGTGGTAAATTc                                                                                                                                                                                                                                                                   <  2:560092/279‑1 (MQ=255)
               aaTGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATg                                                                                                                                                                                                                                                               >  1:1578148/1‑289 (MQ=255)
                 tGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGaa                                                                                                                                                                                                                                                             <  1:682036/289‑1 (MQ=255)
                   cAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCCCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGCGGCAGCGTTTCCTGGGCGGTCAGTCGGGTGACGAGGGAAAGCATCTCATTAAGCGCGGTGTCGGC‑CGACATCAATCCGGTGTTAACTTCCATGaaa                                                                                                                                                                                                                                                            >  2:311611/1‑288 (MQ=255)
                    aGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGaaaa                                                                                                                                                                                                                                                           <  2:1637443/288‑1 (MQ=255)
                      gTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCCCATTAATCGCGGGGTCGAG‑CGACCTCAATCCGGTGGTAAATTCCATGAAAacac                                                                                                                                                                                                                                                        >  2:486604/1‑289 (MQ=255)
                                  catagccgaTGGTGCGGCGGAACGGCAAAGGATGTGCCGGCAACGTGGAAGGGTTGGCCGGAGGCAAACGCGGCGAACCGAACCGCGTAGGAGTCCATTGCGGAGATATCAAACACCGGAACATCAAGCGGCGAAAAGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTACTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGGGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAACACACTCCTGCGGAGGc                                                                                                                                                                                                                                            <  2:1573742/281‑1 (MQ=255)
                                    ttCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCaa                                                                                                                                                                                                                                          >  1:904758/1‑289 (MQ=255)
                                           tGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGACACGACATCGCTCGCCACAATACGACCAAAACACTGTACCAGTGGCCCGCTTTCCTGGGCGTTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCTCGGTGTCGCGCGCTC‑TCAATCAGGTCTTAAATTCCATGACTCCACCCCTGCgtcggctcacgcc                                                                                                                                                                                                                                     >  2:90713/1‑275 (MQ=255)
                                           tGGTAGGGCTGACGGGCAAAGGATTTACCGGAAACGGGCAGCGGTTGCCCGGAGGGAATATCGGCGAAACGCACCGCGTATGCGTCCAATGCGGAGTTAGCAAACCCCGGAGCATCAAGCGGCGAAACGACATCGCGCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGaa                                                                                                                                                                                                                                   <  2:425535/289‑1 (MQ=255)
                                           tGGAATGGCTGACCGGCAACGGATTTACCGGCAACGGGCATCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTTGCAGCTTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGaa                                                                                                                                                                                                                                   <  1:1196522/289‑1 (MQ=255)
                                            gggaTGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGGTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACGCTGGACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGaa                                                                                                                                                                                                                                   <  2:1843392/285‑1 (MQ=255)
                                            ggTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCCTCAAGCTCGGTGTCGAG‑GGACTTCAATCCGGTGCTAAATTCCCTGAAAACACTCCCGCGGAGGCAAACCCGAAt                                                                                                                                                                                                                                  >  2:1314916/1‑289 (MQ=255)
                                            ggTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCAt                                                                                                                                                                                                                                                                >  2:1055035/1‑259 (MQ=255)
                                                tGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTg                                                                                                                                                                                                                               >  1:1162573/1‑288 (MQ=255)
                                                   cTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCta                                                                                                                                                                                                                           >  1:517854/1‑289 (MQ=255)
                                                   cTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCta                                                                                                                                                                                                                           >  1:932420/1‑289 (MQ=255)
                                                        cGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCCTTAAGCCCGGTGGCGAG‑CGACAGCAATCCGGTGGTAAATTCCATGAAAACCCTCCGTCGGGGGCAAAATCGAATTTGCCTATTATg                                                                                                                                                                                                                      >  2:1419120/1‑289 (MQ=255)
                                                        cGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATg                                                                                                                                                                                                                      >  1:568372/1‑289 (MQ=255)
                                                        cGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAACCGAATTTGCCTATTATg                                                                                                                                                                                                                      >  1:851486/1‑289 (MQ=255)
                                                          gCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGc                                                                                                                                                                                                                              <  2:670749/279‑1 (MQ=255)
                                                            aaaGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAg                                                                                                                                                                                                                  >  1:429548/1‑289 (MQ=255)
                                                                 aTTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGaaaa                                                                                                                                                                                                              <  1:17890/288‑1 (MQ=255)
                                                                 aTTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGaaaa                                                                                                                                                                                                              <  1:1206805/288‑1 (MQ=255)
                                                                  tttACCGGCAACGTGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAAc                                                                                                                                                                                                            <  1:129827/289‑1 (MQ=255)
                                                                  tttACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCTGCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAAc                                                                                                                                                                                                            <  1:611785/289‑1 (MQ=255)
                                                                      ccGGCAAGGTGCAGCGGTTGCCCGGAGGACATAGCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTAACAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCca                                                                                                                                                                                                        <  2:282953/289‑1 (MQ=255)
                                                                        ggCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCcaca                                                                                                                                                                                                      <  2:904758/289‑1 (MQ=255)
                                                                        cgcagagggCAGCGGTTGCACGGCAGACATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCcaca                                                                                                                                                                                                      <  2:602477/283‑1 (MQ=255)
                                                                          cAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTCATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAg                                                                                                                                                                                                     >  1:1783327/1‑288 (MQ=255)
                                                                           aaCGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGa                                                                                                                                                                                                    <  2:517854/288‑1 (MQ=255)
                                                                           aaCGGGCAGCGGTTGCACGGGGGCAATATCGGCTAAACGACCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGa                                                                                                                                                                                                    <  2:503770/288‑1 (MQ=255)
                                                                                  aGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCATCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATg                                                                                                                                                                                             <  1:1047519/288‑1 (MQ=255)
                                                                                              gtggCAATTACGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCg                                                                                                                                                                                 <  2:1379046/286‑1 (MQ=255)
                                                                                              gAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCg                                                                                                                                                                                 <  2:800951/288‑1 (MQ=255)
                                                                                               gggCAATATCGGCTAAACCCACCTCTAAGCCGGCCATTGCGGAATTATCCAACGCCGGAACATCAGTCGGCGAGACGACATCGCTCGCCAGAATACGACCAAAACGCTGTACCAGTGGCAGGGGTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGt                                                                                                                                                                                <  2:1237993/287‑1 (MQ=255)
                                                                                                ggCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTa                                                                                                                                                                               <  1:1649605/288‑1 (MQ=255)
                                                                                                ggCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTa                                                                                                                                                                               <  1:1306982/288‑1 (MQ=255)
                                                                                                  cAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAgcg                                                                                                                                                                            <  2:1480479/289‑1 (MQ=255)
                                                                                                    atatCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCt                                                                                                                                                                          <  1:862846/289‑1 (MQ=255)
                                                                                                        ccgCTAAACGCACCCCGTCGCCATCCATTGCGGAGTTATCCAACCCCGGAACATCAAGCGGCAAAACGACCTCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGt                                                                                                                                                                      <  1:762634/287‑1 (MQ=255)
                                                                                                        cGGCTAAACGCACCGCGTAGCCGTCCATGGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACAGCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATGAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATGTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGt                                                                                                                                                                      <  2:1594996/289‑1 (MQ=255)
                                                                                                         ggccagaccgaCCGCCGAGGCGGCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTc                                                                                                                                                                     <  2:1052606/279‑1 (MQ=255)
                                                                                                             aaaCGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGc                                                                                                                                                                 <  1:683534/289‑1 (MQ=255)
                                                                                                                   aCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATc                                                                                                                                                                                                                                                                                >  2:556497/1‑172 (MQ=255)
                                                                                                                   aCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATc                                                                                                                                                                                                                                                                                <  1:556497/172‑1 (MQ=255)
                                                                                                                   aCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTa                                                                                                                                                            <  1:1732800/288‑1 (MQ=255)
                                                                                                                   aCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTAc                                                                                                                                                           <  1:1514850/289‑1 (MQ=255)
                                                                                                                   aCCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTAc                                                                                                                                                           <  1:349889/289‑1 (MQ=255)
                                                                                                                   aCCGCGTAGCCGTCCATTGCGGAGTCATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTa                                                                                                                                                            <  2:1783327/288‑1 (MQ=255)
                                                                                                                     cgcgTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACAt                                                                                                                                                         >  2:640770/1‑289 (MQ=255)
                                                                                                                        gTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTTAGAAAAACGCCACAGAATGTATGCCACCTCGGGCGTAGCGCTGGGCCCTGCCTTCACATGc                                                                                                                                                       >  2:349889/1‑288 (MQ=255)
                                                                                                                          aGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCat                                                                                                                                                    <  1:640770/289‑1 (MQ=255)
                                                                                                                             cGTCCATTGCGGAGATAGCAAAGCCCGGAACATCAAGCGGCGAAACGCCATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGT‑GTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATc                                                                                                                                                 <  2:1690244/288‑1 (MQ=255)
                                                                                                                               tCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCa                                                                                                                                               >  1:1884049/1‑289 (MQ=255)
                                                                                                                                 cATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCAt                                                                                                                                                                                                                                                                <  2:162855/174‑1 (MQ=255)
                                                                                                                                 cATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCAt                                                                                                                                                                                                                                                                >  1:162855/1‑174 (MQ=255)
                                                                                                                                 cATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCatat                                                                                                                                                  >  2:1224378/1‑284 (MQ=255)
                                                                                                                                 cATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCatat                                                                                                                                                  <  1:1224378/284‑1 (MQ=255)
                                                                                                                                    tGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCtt                                                                                                                                           >  1:570005/1‑288 (MQ=255)
                                                                                                                                         aGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCtata                                                                                                                                     >  1:36205/1‑289 (MQ=255)
                                                                                                                                              tCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTcc                                                                                                                                                                    <  1:409757/253‑1 (MQ=255)
                                                                                                                                              tCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCGCGGGCGTAGCGCTGGGTcc                                                                                                                                                                    >  2:409757/1‑253 (MQ=255)
                                                                                                                                                     ccGGAACATCCAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGa                                                                                                                         <  1:827418/289‑1 (MQ=255)
                                                                                                                                                     ccGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGa                                                                                                                         >  1:1385405/1‑289 (MQ=255)
                                                                                                                                                     ccGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCCCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGa                                                                                                                         >  2:1899014/1‑289 (MQ=255)
                                                                                                                                                        gAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCCTCGCGCTGGGTCCTGCCTTTACATGCCATATCCACCTTTCTATATTTAAAATTTGAATg                                                                                                                      >  2:1417208/1‑289 (MQ=255)
                                                                                                                                                                     cgaaaCGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATaa                                                                                                          <  1:490346/285‑1 (MQ=255)
                                                                                                                                                                       aaaCGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATaaaaa                                                                                                       >  1:839926/2‑289 (MQ=255)
                                                                                                                                                                                                    aaaCACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAac                                                                          <  2:36205/289‑1 (MQ=255)
                                                                                                                                                                                                           gTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCtt                                                                   >  1:258173/1‑289 (MQ=255)
                                                                                                                                                                                                             aCCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                 <  1:1000234/289‑1 (MQ=255)
                                                                                                                                                                                                             aCCAGGGGCAGCTTTTCCTGGGTGGGCATGAGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CAACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                 <  2:1162573/289‑1 (MQ=255)
                                                                                                                                                                                                               cAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                               >  1:928060/1‑289 (MQ=255)
                                                                                                                                                                                                                  tGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGAc                                                            >  1:1795440/1‑289 (MQ=255)
                                                                                                                                                                                                                   ggCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGAc                                                            <  1:1877062/288‑1 (MQ=255)
                                                                                                                                                                                                                    gCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTc                                                                                                                                                                     <  1:1515413/182‑1 (MQ=255)
                                                                                                                                                                                                                    gCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTc                                                                                                                                                                     >  2:1515413/1‑182 (MQ=255)
                                                                                                                                                                                                                    gCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGaa                                                                           <  2:1711661/272‑1 (MQ=255)
                                                                                                                                                                                                                    gCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGaa                                                                           >  1:1711661/1‑272 (MQ=255)
                                                                                                                                                                                                                    gCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGa                                                          >  2:1321099/1‑289 (MQ=255)
                                                                                                                                                                                                                     cAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGa                                                          <  1:144071/288‑1 (MQ=255)
                                                                                                                                                                                                                      aGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTCTATTCAAAAATTTAATGAGTAATTCATAAAAATTCTGATATTTATATCAAAATTGGCTAACCACCCTTAATGGAAGact                                                        >  2:1860440/1‑287 (MQ=255)
                                                                                                                                                                                                                              cTGGGAGGTCAGTGAGGGGACGCGAGAAAGGATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCGGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATggg                                                <  2:946080/289‑1 (MQ=255)
                                                                                                                                                                                                                                  gCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCaaa                                            >  1:754779/1‑289 (MQ=255)
                                                                                                                                                                                                                                   gggTCAGGGGGGTGACGCGCGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCaaa                                            <  2:258173/287‑1 (MQ=255)
                                                                                                                                                                                                                                               tGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCaa                               >  1:1052637/1‑289 (MQ=255)
                                                                                                                                                                                                                                               tGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCaa                               >  1:1547988/1‑289 (MQ=255)
                                                                                                                                                                                                                                               tGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCaa                               >  2:1621247/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    cgAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGa                                                                            <  2:1442561/239‑1 (MQ=255)
                                                                                                                                                                                                                                                    cgAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGa                                                                            >  1:1442561/1‑239 (MQ=255)
                                                                                                                                                                                                                                                    cgAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCAt                          >  1:26786/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            gtgtCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCt                                                                                                                                                                                       <  2:841827/108‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gtgtCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGa                                                          >  2:581356/1‑233 (MQ=255)
                                                                                                                                                                                                                                                                            gtgtCGAG‑CGACATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCcgc  >  1:1061662/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
ACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGGCTAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTCAGTGGGGTGACGCGAGAAAGCATCTCATTAAGCGCGGTGTCGAG‑GCTCATCAATCCGGTGGTAAATTCCATGAAAACACTCCTGCGGAGGCAAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGC  >  CP042583/728956‑729512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A