Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 792,696 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583792,6960AT100.0% 572.4 / NA 241intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (144/97);  total (144/97)
*CP042583792,6970CG99.2% 572.0 / NA 241intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (142/96);  total (144/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583792,6980TA100.0% 576.5 / NA 241intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (144/97);  total (144/97)

Only 100 of 241 total aligned reads displayed.

AACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCAACTTTCTGGACTTAAATCGCGTAATTCTATCCCGTTGATTCGTAGGCTTCCCTGATAACTGAGAAAACCAGAAAGCGCGTTCAGCAGACTGCTTTTACCACTACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGGCTCGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTGC‑TTGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCGCAAGGA  >  CP042583/792420‑792973
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                    
aaCAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGa                                                                                                                                                                                                                                                                            <  1:1903718/289‑1 (MQ=255)
   aGGCGTATCAACGCCTGGTGGGAGGAGCGGTAGAAACTTGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGa                                                                                                                                                                                                                                                                            <  2:1299331/286‑1 (MQ=255)
         aTCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTTAACCGCTGCGACcaaccaac                                                                                                                                                                                                                                                                   >  2:1833987/1‑289 (MQ=255)
               gCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGa                                                                                                                                                                                                                                                                            >  2:1567255/1‑274 (MQ=255)
               gCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGc                                                                                                                                                                                                                                                              >  1:1309145/1‑288 (MQ=255)
                   tgtgGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGtt                                                                                                                                                                                                                                                         >  1:292388/1‑289 (MQ=255)
                   tgtgGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCGTATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGAGACCAACCAACACCGCACGtt                                                                                                                                                                                                                                                         >  1:1851104/1‑289 (MQ=255)
                      gggAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGc                                                                                                                                                                                                                                                      <  1:395472/289‑1 (MQ=255)
                           gagCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCct                                                                                                                                                                                                                                                    >  1:219068/1‑286 (MQ=255)
                                           cTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCa                                                                                                                                                                                                                                 >  1:1622791/1‑289 (MQ=255)
                                           cTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTGCAGCAGTGAGCTTTTACCTGAACCGCTGCGAACAACCAACACCGCACGTTGGCCTGCTTGCAAAGTAAAGTTCa                                                                                                                                                                                                                                 >  1:1403485/1‑289 (MQ=255)
                                                ccAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGt                                                                                                                                                                                                                            <  2:187642/289‑1 (MQ=255)
                                                          tCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCg                                                                                                                                                                                                                  >  1:1557761/1‑289 (MQ=255)
                                                               cgcTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGtttt                                                                                                                                                                                                              >  1:178289/1‑288 (MQ=255)
                                                               cgcTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGtttt                                                                                                                                                                                                              >  2:1685534/1‑288 (MQ=255)
                                                                    ctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCtt                                                                                                                                                                                                         >  1:997670/1‑288 (MQ=255)
                                                                         taTTCTTGTTAGCTGGGATCAGGTCGCGCCAGTAGTAAGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGTTCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   <  2:730851/288‑3 (MQ=255)
                                                                         aaTTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   <  1:1178521/289‑3 (MQ=255)
                                                                         aaTTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   <  1:750102/289‑3 (MQ=255)
                                                                         aaTTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   <  2:112892/289‑3 (MQ=255)
                                                                              ttGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcgacgtg                                                                                                                                                                                              >  1:551176/1‑282 (MQ=255)
                                                                              ttGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcgacgtg                                                                                                                                                                                              >  1:1057673/1‑282 (MQ=255)
                                                                                            cAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGctcctc                                                                                                                                                                                <  2:1582811/289‑1 (MQ=255)
                                                                                                 cgcgCCCGTAGTACGTTAGCGCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCt                                                                                                                                                                           <  2:944335/289‑1 (MQ=255)
                                                                                                  gcgcCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCa                                                                                                                                                                                                                     <  2:1788028/246‑1 (MQ=255)
                                                                                                   cgcCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGctactacgccccc                                                                                                                                                                         >  1:11068/1‑281 (MQ=255)
                                                                                                      cattagtaCGTTTTCCCGCAATTTTGCGTCCGGTAATTGTGGGTTGTGCCCAACCCAGGAGAGTTGTTTACGCCATTGTTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTTATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCTCACGTTTGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATg                                                                                                                                                                      <  2:858657/286‑1 (MQ=255)
                                                                                                              cGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCgg                                                                                                                                                              >  2:1008777/1‑289 (MQ=255)
                                                                                                                   tCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   <  2:1440612/247‑3 (MQ=255)
                                                                                                                   tCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   >  1:1440612/1‑245 (MQ=255)
                                                                                                                   tCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCgg                                                                                                                                                         >  1:1582401/1‑289 (MQ=255)
                                                                                                                   tCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGAACgg                                                                                                                                                         >  1:1743022/1‑289 (MQ=255)
                                                                                                                   tCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCCGCCAGCGTTTTAACTTCCGGCTACCTCAACAACAGCTCCTCCGCCCTCATGGTCCCCgcagcg                                                                                                                                                          >  2:1665459/1‑283 (MQ=255)
                                                                                                                    cccGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGt                                                                                                                                                        <  2:785204/289‑1 (MQ=255)
                                                                                                                     ccGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGtc                                                                                                                                                       <  1:389900/289‑2 (MQ=255)
                                                                                                                      cGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGtcg                                                                                                                                                      >  1:151718/1‑287 (MQ=255)
                                                                                                                        cAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGc                                                                                                                                                                             <  1:611611/264‑1 (MQ=255)
                                                                                                                        cAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGGTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGc                                                                                                                                                                             >  2:611611/1‑264 (MQ=255)
                                                                                                                          aTGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGtcgat                                                                                                                                                    >  2:1458387/1‑283 (MQ=255)
                                                                                                                               gctgcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGCTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCACTGGTCACCGGATCGGTCG‑ATGCTAAt                                                                                                                                             >  1:768874/1‑288 (MQ=255)
                                                                                                                                  cccagTACTTGTGGGGTTTGCCCACCCCAGGAGAGCTGTTTACGCCATGATTCTGAGGATAAATCGCGTAATCCTATCCCGTTGATTCGTAGCGATCCCTGATATGGGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCg                                                                                                                                         <  2:1309145/285‑1 (MQ=255)
                                                                                                                                       tAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATC‑GTGCGATGCTAATTCCGcccc                                                                                                                                     >  2:781931/1‑286 (MQ=255)
                                                                                                                                        aagtgtgGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTAGCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTcac                                                                                                                                   <  2:303148/286‑1 (MQ=255)
                                                                                                                                              gggTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGtt                                                                                                                             <  1:1696093/289‑1 (MQ=255)
                                                                                                                                                  tttCCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCTGAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGCGCTTTTACCTGACCCGCTGCGACCAACCAACACCGCACGTTGGACTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCg                                                                                                                          <  1:716321/288‑1 (MQ=255)
                                                                                                                                                    tGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCa                                                                                                                                                                                                                     >  1:69310/1‑196 (MQ=255)
                                                                                                                                                    tGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCa                                                                                                                                                                                                                     <  2:69310/196‑1 (MQ=255)
                                                                                                                                                           cccAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGa                                                                                                                 >  1:1342348/1‑288 (MQ=255)
                                                                                                                                                            ccAGTAGAGATGTTTACGCCATTATTCTTGTGATAAATCGCATAATTCTATCCCGTTTATTCGTAGCTATCCCTGATATGAGAGAAAACCAAAAAGCGCGTTCATCAGTGAGCTTTTACCTGAACCGCTGCGACCACCCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGc                                                                                                               <  1:1135984/289‑1 (MQ=255)
                                                                                                                                                                   agaTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGtt                                                                                                         >  1:1387562/1‑288 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCTACGTGCTAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCTCCTCACCACCTTGCGGTTGGGCGAGCGGGGTTTc                                                                                                       >  2:1066343/1‑288 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGa                                                                                                                                                             <  2:911545/235‑1 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGa                                                                                                                                                             >  1:911545/1‑235 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGtc                                                                                                                                                       <  2:313302/241‑2 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGCGCGGGGTTTcc                                                                                                      >  1:810563/1‑289 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTcc                                                                                                      >  2:1641304/1‑289 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTcc                                                                                                      >  1:101201/1‑289 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTcc                                                                                                      >  1:1001935/1‑289 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTc                                                                                                       >  2:324819/1‑288 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑AGGCGAATTCCGCCTCACCACGTTGCCGATGGGAGACCGGGGTGTca                                                                                                      >  2:581706/1‑288 (MQ=255)
                                                                                                                                                                     aTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGCCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTcc                                                                                                      >  2:239177/1‑289 (MQ=255)
                                                                                                                                                                          tACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATTGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGACCGGGGGTTCCaaaa                                                                                                  >  2:1324902/1‑288 (MQ=255)
                                                                                                                                                                          tACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATaaa                                                                                                 <  2:642975/289‑1 (MQ=255)
                                                                                                                                                                                 tgattctggtgatAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATAGGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGGTCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTc                                                                                          <  2:1530096/277‑1 (MQ=255)
                                                                                                                                                                                         gtgatAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc                                                                                  >  1:946045/5‑289 (MQ=255)
                                                                                                                                                                                         gtgatAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc                                                                                  >  1:533916/5‑289 (MQ=255)
                                                                                                                                                                                             taaagagcgTAATGCTATACGTTTGATTCGTAGCGAGCCCTGATAGGAGAAAAAGCCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCt                                                                              <  2:172633/283‑1 (MQ=255)
                                                                                                                                                                                              aaaTCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGCGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTACAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCACTAAACGTTTTCAGCCTGCCAGCt                                                                              >  2:257073/1‑288 (MQ=255)
                                                                                                                                                                                              aaaTCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCggcg                                                                                                                                                                                                   <  2:957668/172‑3 (MQ=255)
                                                                                                                                                                                              aaaTCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCGATAAACGTTTTCAGACTGTCAGCTg                                                                             >  2:393131/1‑289 (MQ=255)
                                                                                                                                                                                              aaaTCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGATCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCCGAATGCAAGCt                                                                              >  2:820633/1‑288 (MQ=255)
                                                                                                                                                                                                 tCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCGCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCCCACCACGTTGCGGATGGGCTAGTGGGGCTTCCATAAACGTGTTATGACTGTCCGCTggcc                                                                          >  2:1359547/1‑286 (MQ=255)
                                                                                                                                                                                                   gcgTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCa                                                                        <  1:783529/289‑1 (MQ=255)
                                                                                                                                                                                                   acgTAATCCTAACCCGTTGATTCGTAGCGTTCCCCGGTCTGAGCGAAAACCAGAAAGCGCGTTCGGTAGTCAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGATGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCGTCCGGCTACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTGTCCATAAACGTTTTCAGACTGTCAGCTGCGCCa                                                                        <  2:176754/288‑1 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGtc                                                                                                                                                       <  2:474795/195‑2 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGtc                                                                                                                                                       >  1:474795/1‑194 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACg                                                                                                                               <  1:1008577/218‑1 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                                                         >  1:574708/1‑288 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                                                         >  1:45597/1‑288 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTa                                                         >  1:372789/1‑288 (MQ=255)
                                                                                                                                                                                                                   ttGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTGTTCAGACTGTCAGCTGCCCCAACAGCCTGGGCTTTa                                                         >  1:871507/1‑288 (MQ=255)
                                                                                                                                                                                                                    tGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAg                                                        <  1:1286084/288‑1 (MQ=255)
                                                                                                                                                                                                                      aTTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCAGCGCCAACAGCCTGGGCTTTAGCAt                                                     >  1:505558/1‑289 (MQ=255)
                                                                                                                                                                                                                               gatCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATaaaa                                              <  1:1668997/285‑1 (MQ=255)
                                                                                                                                                                                                                                atCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGt                                           >  1:665724/2‑289 (MQ=255)
                                                                                                                                                                                                                                           gagagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGc                                                                   <  2:440653/252‑1 (MQ=255)
                                                                                                                                                                                                                                            ggagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAg                                                                                                                >  1:267185/2‑208 (MQ=255)
                                                                                                                                                                                                                                            agagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCaa                                                                       >  2:1084519/2‑249 (MQ=255)
                                                                                                                                                                                                                                            agagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCaa                                                                       <  1:1084519/248‑1 (MQ=255)
                                                                                                                                                                                                                                            agagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCAAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATcgcg                               >  1:1464723/2‑289 (MQ=255)
                                                                                                                                                                                                                                             gagaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ACGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt                              >  2:203785/1‑289 (MQ=255)
                                                                                                                                                                                                                                              agaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCa                                                                                                                                                                  >  1:1468252/1‑157 (MQ=255)
                                                                                                                                                                                                                                              agaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTa                             >  1:1035424/1‑289 (MQ=255)
                                                                                                                                                                                                                                              agaAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTGCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTa                             >  2:1858894/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            cgcgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGCTAAAACGTACCGAGATCGCGGAATGGCTGGaacaa                >  2:1257275/1‑288 (MQ=255)
                                                                                                                                                                                                                                                            cgcgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGaaaaa                >  2:750813/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             gcgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAACCGTTTTCAGACTGTCAGCTGCGCCAACAGCCGGGGCTTTAGCACGATAAAACGTACCGAGATCGCGTAATGGCCGGAAAAAc               >  2:6738/1‑288 (MQ=255)
                                                                                                                                                                                                                                                              cgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGACG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCATGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGaaaacc               >  1:179269/1‑285 (MQ=255)
                                                                                                                                                                                                                                                              cgTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACACACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGGTCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAAct              <  2:1541658/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      agtgagCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGCGACGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTCG‑ATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCGCa      <  1:1458387/283‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                    
AACAGGCGTATCAACGCCTTGTGGGAGGAGCGGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCAACTTTCTGGACTTAAATCGCGTAATTCTATCCCGTTGATTCGTAGGCTTCCCTGATAACTGAGAAAACCAGAAAGCGCGTTCAGCAGACTGCTTTTACCACTACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTAAAGTTCAGCGGTCCGGCCAGCGTTTTACCTTCCGGGCTCGTGATAAACAGCTCCTCGGCCTCAATGGTCACCGGATCGGTGC‑TTGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGCTGGAAAAACTCTGGCGCAAGGA  >  CP042583/792420‑792973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A