Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,810,226 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,810,2260AT99.2% 611.8 / NA 261intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (120/138);  total (122/139)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,810,2270GC100.0% 611.8 / NA 260intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (121/139);  total (121/139)
*CP0425831,810,2280CG99.6% 611.8 / ‑6.4 260intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (121/138);  minor base A (0/1);  total (121/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 261 total aligned reads displayed.

CGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCAGTTGTTAAATTAATATTTAGAGGGAGCTTGCTCATGTTAACGAAAGA‑TCTTAGCATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTAGCCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCAGCCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCC  >  CP042583/1809950‑1810506
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
gcatgtacggttatcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACttttt                                                                                                                                                                                                                                                                              >  2:1102244‑M2/257‑289 (MQ=255)
    gtacggttatcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTgcg                                                                                                                                                                                                                                                                           >  1:210393‑M2/253‑288 (MQ=255)
    gtacggttatcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTgcg                                                                                                                                                                                                                                                                           >  1:1827532‑M2/253‑288 (MQ=255)
    gtacggttatcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTgcg                                                                                                                                                                                                                                                                           >  1:926570‑M2/253‑288 (MQ=255)
     tacggttatcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgcaagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgacatagttgagggtgacaaaaccgtggtctatgctgttaaaaccgggaaagaagccgccgaggcgactcatcatcagttagagggagcttgtTCATGTTAACGAAAGA‑TCTTTCGATTACTTCTTgcggc                                                                                                                                                                                                                                                                         >  2:1806999‑M2/252‑289 (MQ=255)
            atcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGt                                                                                                                                                                                                                                                                  >  1:1560303‑M2/245‑289 (MQ=255)
             tcgggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTCACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGATGtt                                                                                                                                                                                                                                                                 >  2:31985‑M2/244‑289 (MQ=255)
               gggcgaactgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtcttttctgctggcggtattgttgagggtgacaaaaccggtgtctatgccgtgaaaaccgggaaagaagcctccgtggcgactcataactatttagcgggatcttgaTAATGTTAACGACAGACTTTTAg‑attactatttgcgtggggaatgtc                                                                                                                                                                                                                                                                >  2:1557299‑M2/242‑277 (MQ=255)
                      ctgacgatggcggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAAccc                                                                                                                                                                                                                                                        >  1:1705042‑M2/235‑289 (MQ=255)
                                cggcagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCct                                                                                                                                                                                                                                               <  1:1724977‑M2/64‑1 (MQ=255)
                                   cagataaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCctctct                                                                                                                                                                                                                                           >  1:619499‑M2/222‑289 (MQ=255)
                                       taaaattattctcgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTctcttctt                                                                                                                                                                                                                                       <  1:1081320‑M2/72‑2 (MQ=255)
                                                  tcgccggcggtcaacatgccagactagatgccgtggcggagttagagccgcagagtaaaacaatcaaaacacaaaatgaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagagtgacaaatccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGc                                                                                                                                                                                                                            <  2:1751533‑M2/83‑1 (MQ=255)
                                                    gccgtctgtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                          <  1:320813‑M2/85‑1 (MQ=255)
                                                    gccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                          <  2:1670478‑M2/85‑1 (MQ=255)
                                                    gccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                          <  1:255589‑M2/85‑1 (MQ=255)
                                                    gccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCa                                                                                                                                                                                                                           >  1:754645‑M2/205‑288 (MQ=255)
                                                    cgccgtcggtcagcatgccagactagatgcctttgcggagttagagccgcagcgtaacacatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCaa                                                                                                                                                                                                                          <  1:231225‑M2/85‑1 (MQ=255)
                                                      cgtcggtcagcatgccagactagatgcctttgcggagttagaaccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACt                                                                                                                                                                                                                        <  1:73875‑M2/87‑1 (MQ=255)
                                                                catgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAAGTGCTATGAGAt                                                                                                                                                                                                              >  2:963009‑M2/193‑289 (MQ=255)
                                                                 atgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGAtg                                                                                                                                                                                                             <  1:813766‑M2/98‑1 (MQ=255)
                                                                  tgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaagaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGAtgt                                                                                                                                                                                                            <  2:210393‑M2/99‑1 (MQ=255)
                                                                  tgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGGCTCTCTTCTTCGCCGGTAGGCAACTGCTATg                                                                                                                                                                                                                  >  2:786396‑M2/191‑283 (MQ=255)
                                                                  tgccagactagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGAtgt                                                                                                                                                                                                            <  1:1785631‑M2/99‑1 (MQ=255)
                                                                         ctagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaacattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgaccaaaccgtggtctatgccgtgcacaccgggacagaagccgccgaggcgcttcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGACGTTTCCCAACCCGTTCTGCCTCCCTTCTTCGCCGGTAGGCAACTGCTATGAAATGTGTGCCaa                                                                                                                                                                                                     >  2:800195‑M2/184‑289 (MQ=255)
                                                                         ctagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCaa                                                                                                                                                                                                     >  2:82865‑M2/184‑289 (MQ=255)
                                                                         ctagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatcccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCa                                                                                                                                                                                                      <  1:1531984‑M2/105‑1 (MQ=255)
                                                                          taggagccgttgcggcgtcagagccgcagcgtaccacaatcagaacacagaattaccagacccgcgagccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAACGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCaaa                                                                                                                                                                                                    <  2:400777‑M2/107‑1 (MQ=255)
                                                                          tagatgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCaaa                                                                                                                                                                                                    <  1:1299712‑M2/107‑1 (MQ=255)
                                                                              tgcctttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCt                                                                                                                                                                                                >  1:1663683‑M2/179‑289 (MQ=255)
                                                                                  tttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACg                                                                                                                                                                                             >  2:1703175‑M2/175‑288 (MQ=255)
                                                                                   ttgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGac                                                                                                                                                                                           <  1:1597096‑M2/116‑1 (MQ=255)
                                                                                    tgcggagttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACTCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGaca                                                                                                                                                                                          <  2:733696‑M2/117‑1 (MQ=255)
                                                                                          gttagagccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGt                                                                                                                                                                                     <  1:1013025‑M2/122‑1 (MQ=255)
                                                                                              gagccggagcgtgacaacagcaaagcacaaaagtcccagccccgcgaccggcaagtctttgctgagggcgatattgttgagggtgacaaaaccgtggtctaggccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTggg                                                                                                                                                                                 <  2:1204162‑M2/126‑1 (MQ=255)
                                                                                              aagccggcgcgtaaacccatcacaacacaacatgaccagaaccgcgagccgcacgactttgcgcctagcgatattgttgagggtgacagaaccgtggtctatgccgtgaaaagcgtgaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTTACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATTTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                <  2:168614‑M2/127‑1 (MQ=255)
                                                                                                gccgcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTgggg                                                                                                                                                                                <  1:1410288‑M2/127‑1 (MQ=255)
                                                                                                   gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGTCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCACCTTCTATGAGATGTGTGCCAAAGCCCACGACACAGGTTGGGgcggcg                                                                                                                                                                           >  2:334568‑M2/158‑287 (MQ=255)
                                                                                                   gcagcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTTAAGTTTCCCAAACCGTTCTGCCTGTCTTCTTCGCCTGTAGGCAACCTCCTTGAGAGGTATGCCTAAGCCTACGCCCCAGGTTGGGgcgggg                                                                                                                                                                           >  2:156174‑M2/158‑287 (MQ=255)
                                                                                                     agcgtaacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCggtgt                                                                                                                                                                          <  1:1573923‑M2/133‑1 (MQ=255)
                                                                                                      gattaaccccaccaaaaaccaaaagtacccgacccgcgaccctaaagtctttgatgctggcgatattgttgagggtgacagaaccgtggtgtatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATTTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCggtgtgg                                                                                                                                                                        <  2:1065444‑M2/135‑1 (MQ=255)
                                                                                                         taacaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactacttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTCTGGGATGTGTGCCAAAGCTTACCACACAGGTTGGGGCggtgtggtgt                                                                                                                                                                     >  2:490856‑M2/152‑289 (MQ=255)
                                                                                                           acaccatcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGcctctcttcttcgc                                                                                                                                                                                                                                    <  2:177109‑M2/75‑5 (MQ=255)
                                                                                                               catcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTaaa                                                                                                                                                                >  1:1484063‑M2/146‑288 (MQ=255)
                                                                                                                atcaaaacacaaaattaccagacccgcgacacgcaagtctttgctgatggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggagattcatcactctctagaggtcgcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGCTTACTAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGTTATGAGATGGTTCCCAAAGCCTGAGACACCGGTTGGGGCTGTGTGGCGTTGAAAAc                                                                                                                                                              >  2:1494792‑M2/145‑289 (MQ=255)
                                                                                                                 tcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAACGCCTACGACCCAGGTTGGGGCGGTTTGGTGTTTAAAACg                                                                                                                                                             >  2:1840294‑M2/144‑289 (MQ=255)
                                                                                                                 tcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACTACACAGGTTGGGGCGGTGTGGGGTGTAAAACg                                                                                                                                                             >  2:869497‑M2/144‑289 (MQ=255)
                                                                                                                 tcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGac                                                                                                                                                                                           <  1:1543726‑M2/116‑1 (MQ=255)
                                                                                                                 tcaaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGGTGTGTttaaaagg                                                                                                                                                             >  2:1795474‑M2/144‑287 (MQ=255)
                                                                                                                   aaaacaccaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGAt                                                                                                                                                           <  1:1504617‑M2/148‑1 (MQ=255)
                                                                                                                   aaaacacaaaattaccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgatgcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGAt                                                                                                                                                           <  1:277665‑M2/148‑1 (MQ=255)
                                                                                                                               taccagccccgcgccacccaaagctttgctgctggcggtattgtggagggtgacaaaaccgtggtatatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGGTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTAt                                                                                                                                               <  2:671174‑M2/160‑1 (MQ=255)
                                                                                                                                 ccagacccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCg                                                                                                                                             <  1:516211‑M2/162‑1 (MQ=255)
                                                                                                                                      cccgcgacccgcaagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAAc                                                                                                                                        >  1:85328‑M2/123‑289 (MQ=255)
                                                                                                                                                  aagtctttgctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACgaagtctcgcc                                                                                                                             <  2:1150526‑M2/178‑6 (MQ=255)
                                                                                                                                                      cttggctgctggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGtt                                                                                                                        <  1:656742‑M2/183‑1 (MQ=255)
                                                                                                                                                               tggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGatcatc                                                                                                               <  1:547507‑M2/192‑1 (MQ=255)
                                                                                                                                                               tggcgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGatcat                                                                                                                <  1:568486‑M2/191‑1 (MQ=255)
                                                                                                                                                                  cgatattgttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTg                                                                                                             <  1:896321‑M2/194‑1 (MQ=255)
                                                                                                                                                                     ttttgttgagtatgaccaaaccgtggtctatgccgtgaaaaccgtgaaagcagccgccgaggctattcatcactatttagagggagcttgcTCATGTTAACTAAATA‑TCTTTCGATTTCTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGa                                                                                                         <  2:243215‑M2/198‑1 (MQ=255)
                                                                                                                                                                         gttgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAaaga                                                                                                     <  2:1296506‑M2/202‑1 (MQ=255)
                                                                                                                                                                           tgagggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAaagaag                                                                                                   >  1:1869931‑M2/86‑289 (MQ=255)
                                                                                                                                                                             agggtgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGCAGAt                                                                                                 >  2:508069‑M2/84‑289 (MQ=255)
                                                                                                                                                                                 tgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCg                                                                                             >  2:1207079‑M2/80‑289 (MQ=255)
                                                                                                                                                                                 tgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCg                                                                                             >  1:87894‑M2/80‑289 (MQ=255)
                                                                                                                                                                                 tgacaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCg                                                                                             >  1:1348357‑M2/80‑289 (MQ=255)
                                                                                                                                                                                   acaaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGc                                                                                                                                                                                                                            >  2:1014555‑M2/78‑160 (MQ=255)
                                                                                                                                                                                    caaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGtt                                                                                          >  1:1386106‑M2/77‑289 (MQ=255)
                                                                                                                                                                                    caaaaccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGtt                                                                                          >  1:1097005‑M2/77‑289 (MQ=255)
                                                                                                                                                                                         ccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGCTTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGGCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGAGACCGGTTTTatt                                                                                     >  2:597543‑M2/72‑288 (MQ=255)
                                                                                                                                                                                         ccgtggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATc                                                                                     <  1:1231366‑M2/218‑1 (MQ=255)
                                                                                                                                                                                            tggtctatgccgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCg                                                                                    >  2:1530261‑M2/69‑287 (MQ=255)
                                                                                                                                                                                                      cgtgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAata                                                                        >  1:1892303‑M2/59‑289 (MQ=255)
                                                                                                                                                                                                        tgaaaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATg                                                                      >  2:466676‑M2/57‑289 (MQ=255)
                                                                                                                                                                                                           caaccgggaaagaagccgccgaggcgattcatcactaattggggggggcttgcTACTGGTAACGAAGGT‑TCTTTCGATTACTTTTTGCGGGGTGAAGTTTCCCAACCCGTTCGGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCACAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAg                                                                   <  2:1444126‑M2/236‑1 (MQ=255)
                                                                                                                                                                                                           aaaccgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAg                                                                   <  1:490856‑M2/236‑1 (MQ=255)
                                                                                                                                                                                                             accgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCa                                                                 <  2:1532118‑M2/238‑1 (MQ=255)
                                                                                                                                                                                                               cgggaaagaagccgccgaggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGa                                                               >  2:653948‑M2/50‑289 (MQ=255)
                                                                                                                                                                                                                              cgatgctattcatccctatttagagggagcttgcTCATGTTAACGAACGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGt                                                <  1:1208975‑M2/255‑1 (MQ=255)
                                                                                                                                                                                                                                 ggcgattcatcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTgg                                             >  1:126185‑M2/32‑289 (MQ=255)
                                                                                                                                                                                                                                    gattcatcactatttgtagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTTCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGaa                                           <  1:41852‑M2/260‑1 (MQ=255)
                                                                                                                                                                                                                                          tcactatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACgaagtctcgcc                                                                                                                             >  2:1594384‑M2/23‑195 (MQ=255)
                                                                                                                                                                                                                                             ctatttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTgg                                 <  1:466676‑M2/270‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttagggggatcttgcTAATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGgccgc                             <  1:869497‑M2/274‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttagagggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACa                                                     >  1:823627‑M2/16‑265 (MQ=255)
                                                                                                                                                                                                                                                  ttgagggagtgtgaTCATGTTAACGTGAGA‑TCTTGCGAGTTTTTTTTGCGTCGAGAAGTCTCCCGACCCGTTCTGCCTCTCTTCTTAGCCGGTTGGCAACTGCTATGAGATGTGTGCCAACGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGGTCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCATTTTGATCATGGGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGGGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGgccgcc                            <  2:1086676‑M2/275‑1 (MQ=255)
                                                                                                                                                                                                                                                       ggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGgccgcc                            >  1:958266‑M2/10‑284 (MQ=255)
                                                                                                                                                                                                                                                       ggagcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCg                       >  2:915309‑M2/10‑289 (MQ=255)
                                                                                                                                                                                                                                                          gcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAagtctcg                                                                                                                               >  1:1765875‑M2/7‑179 (MQ=255)
                                                                                                                                                                                                                                                          gcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCg                    >  2:118838‑M2/7‑289 (MQ=255)
                                                                                                                                                                                                                                                          gcttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCg                    >  1:71173‑M2/7‑289 (MQ=255)
                                                                                                                                                                                                                                                          gCTTGCTCACGTTAACGAAAGA‑TCTAACGACTACTTTTTGAGGCGTGAAGTTTCCCAACTCGTTATGCCTCTCTTCTTCCCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCTTACGACAGGGGTTGGGGCGGTTTGGTGTTTAAAACGCACGTCTTCTTTATCGACAACGAAGTCTCGCGGCGTTTTTATCATCTGATGAAATAAGATACCGGTTTTAACGCCTAAAATAATATGAAGTAGAGTGCTGAACATAAGTTTGAAGAGAAACTGGCAGACCTGCGACg                    >  2:1251245/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            ttgcTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGc                  <  2:1348357‑M2/285‑1 (MQ=255)
                                                                                                                                                                                                                                                             tgcTCATGTTAACGAAAGA‑GCTTTCGATTACTTTTTGCGGCGTGATGTTTCCCACCCCGTTCTGCCTCTCTTCTTCGCCGATAGGCAACTGCTATGAGATGTTTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTCATCGCCAACGCAGTCTCGCCGCGTTGTGATCATCTGGTGAAAGAAGATAACGGTTTTATCGGCTTCAAAAATATGGAGCCGATTGCTGAACATCCGTTGGACGAGAATCTGGCCGCCCTGCTGCGGc                  >  2:752648‑M2/4‑288 (MQ=255)
                                                                                                                                                                                                                                                               cTCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCct                                                                                                                                                                                                                                               <  2:817840‑M2/64‑1 (MQ=255)
                                                                                                                                                                                                                                                                tCATGTTACAGAAAGA‑TCTTTCGCTTACTTTTTGCGGCGTTAAGTTTCCCAACCCGTTCTTCCTCGCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTgaa              <  2:705087/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                tCATGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTgaa              <  2:87894/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tGTTAACGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTgaagga           >  1:1144567/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         cGAAAGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGAtt      >  2:49966/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                            aaGA‑TCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCCGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTAccc  <  1:1031446/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                      
CGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCAGTTGTTAAATTAATATTTAGAGGGAGCTTGCTCATGTTAACGAAAGA‑TCTTAGCATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTAGCCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCAGCCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCC  >  CP042583/1809950‑1810506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A