Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,925,644 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,925,6440AT99.1% 508.0 / NA 219intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (116/99);  minor base G (0/2);  total (117/102)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,925,6450GC99.5% 502.3 / NA 219intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (116/100);  total (117/102)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,925,6460TA99.1% 506.9 / NA 219intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (115/100);  minor base C (1/1);  total (117/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CGTCACGACGTCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCAGTTCCGGAAGCCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTAGCCCAGAAGTCCCCGTCTGGCC  >  CP042583/1925366‑1925921
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                    
ccgcACGACGTCCGGGCAATCTCTCGGGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCTTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACGGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGatc                                                                                                                                                                                                                                                                              <  1:1352395/286‑3 (MQ=255)
   cACGACGTCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGatcgc                                                                                                                                                                                                                                                                            <  1:447612/288‑5 (MQ=255)
    acgacgTCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGatcgctg                                                                                                                                                                                                                                                                          >  1:1768524/1‑283 (MQ=255)
          tCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGAttt                                                                                                                                                                                                                                                                     >  2:886931/1‑288 (MQ=255)
            cgcgCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCt                                                                                                                                                                                                                                                                  <  1:89353/289‑1 (MQ=255)
                      aTCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCCGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTTGTCAAAACGCACTGCATTTCTCATCCCGATCGCTGGATTTGCTggctcatct                                                                                                                                                                                                                                                         >  2:1495481/1‑280 (MQ=255)
                               cTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGt                                                                                                                                                                                                                                               <  1:791406/289‑1 (MQ=255)
                                   ccaacgtccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAAc                                                                                                                                                                                                                                           <  2:840217/283‑1 (MQ=255)
                                         tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGTTg                                                                                                                                                                                                                                      >  2:570412/1‑288 (MQ=255)
                                         tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                     <  1:659690/289‑1 (MQ=255)
                                         tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTg                                                                                                                                                                                                                                      >  1:316895/1‑288 (MQ=255)
                                         tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGACTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                     <  1:1242242/289‑1 (MQ=255)
                                           cttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                   <  1:121483/289‑1 (MQ=255)
                                           cttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                   <  1:70458/289‑1 (MQ=255)
                                           cttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                   <  2:269682/289‑1 (MQ=255)
                                                  aacgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTc                                                                                                                                                                                                                            >  1:461840/1‑289 (MQ=255)
                                                   acgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTca                                                                                                                                                                                                                           <  2:537909/289‑1 (MQ=255)
                                                    cgCTGGAAAGGCGTGCAACTCACCGCCTGGGGAGCTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTAGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGTCCTCGCCGGATTACTCTGTCGCCTGCACCTTCAAACACACGGCCTGCGCTGTGCTATTGTCACTCTTCTGCAAAGGGCACTGCTTGTTTATGTCGGATCCCTGGATGTGCTGAACCATCTGCTATATGCTCAACCGGttgtggacaatcat                                                                                                                                                                                                                          >  2:789363/1‑276 (MQ=255)
                                                    cgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGCCATTcac                                                                                                                                                                                                                          >  2:873762/1‑289 (MQ=255)
                                                      cTGGAAAGGCGGGCAACCCAGCGCCTGGGGGGATGCGCTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                         <  2:1574822/288‑1 (MQ=255)
                                                           aaGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGa                                                                                                                                                                                                                    <  1:413133/288‑1 (MQ=255)
                                                                               gggggAGATGGACTGCGCGAAAGCGAATTTGCCCGCTGGTTTTATCAGGGATTTTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTGAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTcagca                                                                                                                                                                                                <  2:1324066/287‑1 (MQ=255)
                                                                                   gagaTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCa                                                                                                                                                                                            <  1:901140/288‑1 (MQ=255)
                                                                                    agaTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAc                                                                                                                                                                                           <  2:1736814/288‑1 (MQ=255)
                                                                                    agaTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAc                                                                                                                                                                                           <  2:701338/288‑1 (MQ=255)
                                                                                      aTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGAACTTCATTTCCCCTCCGGATCGCTCGATTTGCTGACCCCACTCCCAGTTGGTCAACCGGTGACCGAACTTCCCAGTTGACTGGAATCTTTTCGTCAGCAGccacca                                                                                                                                                                                         >  2:725459/1‑288 (MQ=255)
                                                                                       tGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGTTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAg                                                                                                                                                                                        >  1:1776464/1‑288 (MQ=255)
                                                                                       tGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAg                                                                                                                                                                                        >  2:674563/1‑288 (MQ=255)
                                                                                       tGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAg                                                                                                                                                                                        >  1:497881/1‑288 (MQ=255)
                                                                                       tGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGc                                                                                                                                                                                       <  2:287584/289‑1 (MQ=255)
                                                                                        tgagtGCGCGAAAGCAAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGGGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                      <  2:31931/285‑1 (MQ=255)
                                                                                        gCACTGCGCGGAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                      <  2:461840/289‑1 (MQ=255)
                                                                                        gCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGGTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGc                                                                                                                                                                                       <  2:1298439/288‑1 (MQ=255)
                                                                                            tGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCAt                                                                                                                                                                                   >  1:1322439/1‑288 (MQ=255)
                                                                                              cgcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCCTTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAAGCTTTGCGTCCGCAGGCCCCAGTCCATCCt                                                                                                                                                                                >  2:1105719/1‑289 (MQ=255)
                                                                                              cgcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCa                                                                                                                                                                                    <  2:296641/285‑1 (MQ=255)
                                                                                                   aaGCGAATTTACCCGCCGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTTCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGCCATCCGGTGACCGACATGCCCAGTTGTCTGGAATCTTTGCGTCGGCCGGCCCCAGCCCATccctccc                                                                                                                                                                            >  2:1819444/1‑283 (MQ=255)
                                                                                                                           tCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCt                                                                                                                                                   <  1:587425/289‑1 (MQ=255)
                                                                                                                               ggATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGa                                                                                                                                                >  1:27441/1‑288 (MQ=255)
                                                                                                                                 aTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAtc                                                                                                                                              >  1:393732/1‑288 (MQ=255)
                                                                                                                                         gTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTCTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCCCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCCGGACTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTTCCAGTGGACTGGCATCTTTGCGTCAGCAGGCACCCGCCCATCCTTCCTCCCTCCCCCACCACCAACGCCTGCCCGACCTC‑CCTTccc                                                                                                                                    >  2:935109/1‑289 (MQ=255)
                                                                                                                                               agaAGGAGCAGAAAGATGCGCTTTGATACTGTCTTTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               <  1:1542510/288‑1 (MQ=255)
                                                                                                                                               agaAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               <  1:570412/288‑1 (MQ=255)
                                                                                                                                                gaAGGAGCAGAAAGATGCGCGTGGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGAGCTGTGCCATTGTCACGCGTGGTCAAAGCGCACTGCATTTCGCATCCGGATCGCTGGATTTGCGGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCAAAACGCGTGCTCGATCTC‑GCTTGCCAGgcgc                                                                                                                              <  2:58336/288‑1 (MQ=255)
                                                                                                                                                    gAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                   <  2:1241887/184‑1 (MQ=255)
                                                                                                                                                    gAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACCACGCGTGCTCGATCTC‑GCTTGCCCGGCGCAGGCa                                                                                                                         >  1:90880/1‑289 (MQ=255)
                                                                                                                                                        aGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCGTTGCGTCAGCAGGCAACAGCCCATCCTTACTCCCTTCTCGATCCACAACGCGTTCTCGA‑CTCTGCTTGCCAGGCGCAGCAATTat                                                                                                                     >  2:607298/1‑288 (MQ=255)
                                                                                                                                                                gcgcTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGaa                                                                                                             >  2:1338021/1‑289 (MQ=255)
                                                                                                                                                                          aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   <  1:788740/289‑1 (MQ=255)
                                                                                                                                                                          aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   <  1:369224/289‑1 (MQ=255)
                                                                                                                                                                          aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTACGCTGGGCCATTGTCACTCGTGGTCAAATCGCACTGCATTTCTCATCCGGATCGCTGGAGTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCAAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATGAATCGCTGAAAGCGGTgcgc                                                                                                   <  1:1105719/289‑1 (MQ=255)
                                                                                                                                                                             gTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCCCGACCCACAACGCGTGCTCGATCCC‑CCTTCCCCGCCGCCGGACTTACTCGCTGCAACCGGCGCGCatt                                                                                                >  2:1282257/1‑287 (MQ=255)
                                                                                                                                                                              tCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGACTCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACTACGCGTGCTCCATCCC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAAtt                                                                                               >  2:113608/1‑289 (MQ=255)
                                                                                                                                                                              tCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTC‑‑‑TCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg                                                                                                      <  1:1710103/279‑1 (MQ=255)
                                                                                                                                                                                aTTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGc                                                                                             >  1:248442/1‑289 (MQ=255)
                                                                                                                                                                                    tGGGCGGCGGCCTCGCCTGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  1:1461472/289‑1 (MQ=255)
                                                                                                                                                                                    tGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  2:432853/289‑1 (MQ=255)
                                                                                                                                                                                      ggcggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGTCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGTATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTACGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGGACTACAACCGGTGCTCGATCTC‑GCGTGCCCGGCGCCGGAATTAACACCCGAAAGCGGTGCGCACTGGCAggcga                                                                                       >  2:1465544/1‑286 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCa                                                                                                                                                                                            >  1:1174306/1‑187 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCa                                                                                                                                                                                            <  2:1174306/187‑1 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTa                                                                                                                                                                              <  1:1042213/201‑1 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCt                                                                                                                                                   <  1:846067/228‑1 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCa                                                                                                                                   <  1:623974/243‑1 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               <  2:1387884/247‑1 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               >  1:1387884/1‑247 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAg                                                                                                           <  2:417288/267‑1 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGc                                                                                     >  1:192684/1‑289 (MQ=255)
                                                                                                                                                                                        cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGc                                                                                     >  1:979513/1‑289 (MQ=255)
                                                                                                                                                                                          gcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCACTCGCCGGCATTAATCGCTGAAAGCGGTGCGCAATTGCATGGCAGCg                                                                                    >  2:1544931/1‑288 (MQ=255)
                                                                                                                                                                                           cggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgacag                                                                                                                                                                                                                                  >  1:365430/1‑146 (MQ=255)
                                                                                                                                                                                               cTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAAc                                                                              <  1:1655117/289‑1 (MQ=255)
                                                                                                                                                                                                       aTTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCa                                                                       >  1:1719477/1‑288 (MQ=255)
                                                                                                                                                                                                         tACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCa                                                                    <  1:1495481/289‑1 (MQ=255)
                                                                                                                                                                                                         cactttggtgccgacaactgcaaAAACACGGCCTGAGCTTGGCCATTGTCACTCGTGGTCAAAGCGCAATGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCa                                                                    <  2:1861649/275‑1 (MQ=255)
                                                                                                                                                                                                           ctctGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   >  2:640535/1‑256 (MQ=255)
                                                                                                                                                                                                                tgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGtt                                                             >  2:1475304/1‑289 (MQ=255)
                                                                                                                                                                                                                 ggCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGt                                                                                   >  1:687341/1‑266 (MQ=255)
                                                                                                                                                                                                                 ggCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGtt                                                             >  1:644722/1‑288 (MQ=255)
                                                                                                                                                                                                                    ctgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcg                                                          <  1:1614109/288‑1 (MQ=255)
                                                                                                                                                                                                                    ctgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcg                                                          <  1:749169/288‑1 (MQ=255)
                                                                                                                                                                                                                      gcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTGAcgcc                                                        >  1:1855126/1‑288 (MQ=255)
                                                                                                                                                                                                                       caactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGcc                                                                                                                                                                                                                                                             <  2:22292/91‑1 (MQ=255)
                                                                                                                                                                                                                       caactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGcc                                                                                                                                                                                                                                                             >  1:22292/1‑91 (MQ=255)
                                                                                                                                                                                                                          ctgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  1:99920/251‑1 (MQ=255)
                                                                                                                                                                                                                          ctgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCg                                                   >  1:1439813/1‑289 (MQ=255)
                                                                                                                                                                                                                               aaaaCACAGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCAct                                              <  2:649283/289‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGGCCTGCGCTCTGACATGGTCACTCTTGGTCAAAGCGCACTGCATTTCTCATCCGGGTCGCTTGATTGGATGAGCCATCTGCCAGATGGTCAACAGGTGACAGACATTCACAGTGGACGGGAATCTTTGCGGCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTgcg                                         <  2:90880/288‑1 (MQ=255)
                                                                                                                                                                                                                                     cGGCCTGGCGTGGGCCGTTGTCATCCGGGGTCAAAGCGCACTAGATTTCTCATCCGGATCGCGGGATTTGCTGGGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTgcgc                                        <  2:979513/289‑1 (MQ=255)
                                                                                                                                                                                                                                          tGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTAc                                   <  2:316895/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 tgCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCCCTACCTGGCTa                            >  1:743775/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   ccATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGTTCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAg                          <  2:745963/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCCCTACCTGGCTAAGtc                        >  2:1043455/1‑287 (MQ=255)
                                                                                                                                                                                                                                                         tcccTCGTGGTCAACGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGTACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGttcgcc                    <  2:623578/286‑6 (MQ=255)
                                                                                                                                                                                                                                                         tCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGttcgcc                    >  1:765638/1‑284 (MQ=255)
                                                                                                                                                                                                                                                           aCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTTCAGGGCAGCGTAGACCTTGCTCACCAGCGGGTTACGCCGCCCGGCACTCTTCGCTCTACCTGCCTAAGttcgccat                  >  2:1680330/1‑282 (MQ=255)
                                                                                                                                                                                                                                                              cGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAg               >  1:1218609/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                tGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  2:1360724/213‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcg                                                          <  1:1002840/233‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGcc  >  1:1136913/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            aCTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         >  1:1166074/1‑201 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                    
CGTCACGACGTCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCAGTTCCGGAAGCCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTAGCCCAGAAGTCCCCGTCTGGCC  >  CP042583/1925366‑1925921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A