Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,925,653 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,925,6530AT100.0% 508.6 / NA 215intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (116/99);  total (116/99)
*CP0425831,925,6540GC100.0% 510.7 / NA 215intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (116/99);  total (116/99)
*CP0425831,925,6550CG99.5% 502.2 / NA 215intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (115/99);  total (116/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

TCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCAGTTCCGGAAGCCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTAGCCCAGAAGTCCCCGTCTGGCC  >  CP042583/1925376‑1925921
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                           
tCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGAttt                                                                                                                                                                                                                                                                     >  2:886931/1‑288 (MQ=255)
  cgcgCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCt                                                                                                                                                                                                                                                                  <  1:89353/289‑1 (MQ=255)
                     cTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGt                                                                                                                                                                                                                                               <  1:791406/289‑1 (MQ=255)
                               tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                     <  2:538051/289‑1 (MQ=255)
                               tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                     <  1:1535478/289‑1 (MQ=255)
                               tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGACTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                     <  1:1242242/289‑1 (MQ=255)
                                 cttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                   <  1:70458/289‑1 (MQ=255)
                                       gaacgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACAtt                                                                                                                                                                                                                             <  1:224335/289‑1 (MQ=255)
                                         acgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTCCTCATCCGGATCGCTGGACTTGCTGAGCCATCTGCCAGATGTTCAACCGGTTACAAACATTc                                                                                                                                                                                                                            >  2:1614109/1‑288 (MQ=255)
                                          cgCTGGAAAGGCGTGCAACTCACCGCCTGGGGAGCTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTAGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGTCCTCGCCGGATTACTCTGTCGCCTGCACCTTCAAACACACGGCCTGCGCTGTGCTATTGTCACTCTTCTGCAAAGGGCACTGCTTGTTTATGTCGGATCCCTGGATGTGCTGAACCATCTGCTATATGCTCAACCGGttgtggacaatcat                                                                                                                                                                                                                          >  2:789363/1‑276 (MQ=255)
                                          cgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGCCATTcac                                                                                                                                                                                                                          >  2:873762/1‑289 (MQ=255)
                                          cgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCAGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTTTGCCCTTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCAACCGCCAGATGGTCAACCGGTGACAGACAATcac                                                                                                                                                                                                                          >  2:587425/1‑289 (MQ=255)
                                           gCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                         >  1:40837/1‑289 (MQ=255)
                                           gCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                         <  1:343276/289‑1 (MQ=255)
                                            cTGGAAAGGCGGGCAACCCAGCGCCTGGGGGGATGCGCTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                         <  2:1574822/288‑1 (MQ=255)
                                                       tGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGaa                                                                                                                                                                                                              >  1:745963/1‑288 (MQ=255)
                                                       tGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGCCAACCGGTGACAGACATTCACCGTGGACTGGaa                                                                                                                                                                                                              >  2:648755/1‑288 (MQ=255)
                                                                  gCCTGGGGAGATGCACTGAGCGAAAGCGAATTTTCCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGGGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTcag                                                                                                                                                                                                  <  2:317059/289‑1 (MQ=255)
                                                                         gagaTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCa                                                                                                                                                                                            <  1:901140/288‑1 (MQ=255)
                                                                          agaTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAc                                                                                                                                                                                           <  2:701338/288‑1 (MQ=255)
                                                                            aTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGAACTTCATTTCCCCTCCGGATCGCTCGATTTGCTGACCCCACTCCCAGTTGGTCAACCGGTGACCGAACTTCCCAGTTGACTGGAATCTTTTCGTCAGCAGccacca                                                                                                                                                                                         >  2:725459/1‑288 (MQ=255)
                                                                             tGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGTTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAg                                                                                                                                                                                        >  1:1776464/1‑288 (MQ=255)
                                                                              gCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                      <  1:385673/289‑1 (MQ=255)
                                                                                aCTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAATATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGccc                                                                                                                                                                                     <  2:1287858/288‑1 (MQ=255)
                                                                                aCTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCa                                                                                                                                                                                    <  1:1202063/289‑1 (MQ=255)
                                                                                  tGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCAt                                                                                                                                                                                   >  1:1322439/1‑288 (MQ=255)
                                                                                     gcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCt                                                                                                                                                                                <  2:68447/288‑1 (MQ=255)
                                                                                         aaGCGAATTTACCCGCTGGGTTTATCAGGGGTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACt                                                                                                                                                                            <  2:1322439/288‑1 (MQ=255)
                                                                                              aaTTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATCTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCtt                                                                                                                                                                       >  2:221561/1‑288 (MQ=255)
                                                                                                                 tCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCt                                                                                                                                                   <  1:587425/289‑1 (MQ=255)
                                                                                                                           tgtgGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCTTTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGCTCGCAGGCACCAGCCCATCCTTACTCCCTTCTCGACCCACAACGCGTGCCCGAtcacc                                                                                                                                           >  2:1542510/1‑284 (MQ=255)
                                                                                                                           tgtgGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTctactccgct                                                                                                                                         >  1:61529/1‑280 (MQ=255)
                                                                                                                           tgtgGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCt                                                                                                                                        >  2:64976/1‑289 (MQ=255)
                                                                                                                               gTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTCTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCCCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCCGGACTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTTCCAGTGGACTGGCATCTTTGCGTCAGCAGGCACCCGCCCATCCTTCCTCCCTCCCCCACCACCAACGCCTGCCCGACCTC‑CCTTccc                                                                                                                                    >  2:935109/1‑289 (MQ=255)
                                                                                                                                tCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTTTGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGGGCTCGATCTC‑CCTTGCCa                                                                                                                                   >  1:296217/1‑289 (MQ=255)
                                                                                                                                 cTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCa                                                                                                                                   <  1:221561/288‑1 (MQ=255)
                                                                                                                                     agaAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               <  2:313105/288‑1 (MQ=255)
                                                                                                                                     agaAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               <  1:570412/288‑1 (MQ=255)
                                                                                                                                         ggAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGc                                                                                                                          >  1:680162/1‑289 (MQ=255)
                                                                                                                                          gAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                   <  2:1241887/184‑1 (MQ=255)
                                                                                                                                          gAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACCACGCGTGCTCGATCTC‑GCTTGCCCGGCGCAGGCa                                                                                                                         >  1:90880/1‑289 (MQ=255)
                                                                                                                                            gCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                      >  1:26999/1‑227 (MQ=255)
                                                                                                                                            gCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCGGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                      <  2:26999/227‑1 (MQ=255)
                                                                                                                                              aGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTa                                                                                                                      >  2:1466910/1‑288 (MQ=255)
                                                                                                                                              aGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCGTTGCGTCAGCAGGCAACAGCCCATCCTTACTCCCTTCTCGATCCACAACGCGTTCTCGA‑CTCTGCTTGCCAGGCGCAGCAATTat                                                                                                                     >  2:607298/1‑288 (MQ=255)
                                                                                                                                                    aTGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTg                                                                                                               >  1:1835841/1‑289 (MQ=255)
                                                                                                                                                      gcgcTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGaa                                                                                                             >  2:1338021/1‑289 (MQ=255)
                                                                                                                                                             ggtACTGTCCTTATGGGCGGCGGCCTCGCCGGATTACTCGGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg                                                                                                      <  2:1717816/287‑1 (MQ=255)
                                                                                                                                                                aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   <  1:788740/289‑1 (MQ=255)
                                                                                                                                                                aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   <  2:497881/289‑1 (MQ=255)
                                                                                                                                                                aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTACGCTGGGCCATTGTCACTCGTGGTCAAATCGCACTGCATTTCTCATCCGGATCGCTGGAGTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCAAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATGAATCGCTGAAAGCGGTgcgc                                                                                                   <  1:1105719/289‑1 (MQ=255)
                                                                                                                                                                aCTGTCATTATGGACGTCGGCTCGGCCGGTGTACTCTGGGGCCTGAACATGCAAAATCCGGGGCTGCGCTGGTCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCGGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   <  2:1455986/289‑1 (MQ=255)
                                                                                                                                                                   gTCATTATGGGCCGCGTCCTCGCCGGCTTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGCCTGACATTACCAGTGGACTGGAATCTTTGCGTCAGCAGGTACCAGCCCATCCTTACTCACTTCTCGAGCCACAACGCGTGCTCGACCTC‑CCTTTCCCGCCTCAGCCATTCATCGCTGCAACCGGtcgtcatt                                                                                                >  2:1088560/1‑282 (MQ=255)
                                                                                                                                                                    tCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGACTCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACTACGCGTGCTCCATCCC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAAtt                                                                                               >  2:113608/1‑289 (MQ=255)
                                                                                                                                                                    tCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCCAtt                                                                                               >  1:1861649/1‑289 (MQ=255)
                                                                                                                                                                    tCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTC‑‑‑TCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg                                                                                                      <  1:1710103/279‑1 (MQ=255)
                                                                                                                                                                      aTTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAAACGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAACAGCCCATCCTTCCTCCCTTCACGAGCCACAAACCCTGCTAGATCTC‑GCTTGCCAGCCGCAGGCATTAATAGCTGAAAACGGTCCGTAATTGc                                                                                             >  2:296010/1‑289 (MQ=255)
                                                                                                                                                                          tGGGCGGCGGCCTCGCCTGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  1:1461472/289‑1 (MQ=255)
                                                                                                                                                                          tGGGCCGCGGCCACGCCGGAGTCCTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGGACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGAGGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTATGCGCCAGCAGGCACCAGCCCATCGTTACCCCCTGTTCGAGCCACAACGCGTGCTCGCGCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  2:57251/289‑1 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCa                                                                                                                                                                                            <  2:1174306/187‑1 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCa                                                                                                                                                                                            >  1:1174306/1‑187 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTa                                                                                                                                                                              >  2:1042213/1‑201 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTa                                                                                                                                                                              <  1:1042213/201‑1 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCt                                                                                                                                                   <  1:846067/228‑1 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCt                                                                                                                                                   >  2:846067/1‑228 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               <  2:1387884/247‑1 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGgcg                                                                                                                               >  1:1387884/1‑247 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAg                                                                                                           >  1:417288/1‑267 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAg                                                                                                           <  2:417288/267‑1 (MQ=255)
                                                                                                                                                                              cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGc                                                                                     >  1:979513/1‑289 (MQ=255)
                                                                                                                                                                                 cggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgacag                                                                                                                                                                                                                                  <  2:365430/146‑1 (MQ=255)
                                                                                                                                                                                  ggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAg                                                                                 >  1:366783/1‑289 (MQ=255)
                                                                                                                                                                                     cTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAAc                                                                              <  1:1655117/289‑1 (MQ=255)
                                                                                                                                                                                             aTTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCa                                                                       >  1:1719477/1‑288 (MQ=255)
                                                                                                                                                                                                 ctctGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                   <  1:640535/256‑1 (MQ=255)
                                                                                                                                                                                                    tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCggg                                                               >  2:1266429/1‑289 (MQ=255)
                                                                                                                                                                                                          ctgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcg                                                          <  1:1614109/288‑1 (MQ=255)
                                                                                                                                                                                                           tgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCCATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcgcc                                                        >  1:462924/1‑289 (MQ=255)
                                                                                                                                                                                                            gcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACCACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcgcc                                                        >  1:454359/1‑288 (MQ=255)
                                                                                                                                                                                                                ctgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  1:99920/251‑1 (MQ=255)
                                                                                                                                                                                                                ctgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCg                                                   >  1:1439813/1‑289 (MQ=255)
                                                                                                                                                                                                                       aaCACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCActct                                            <  1:64976/289‑1 (MQ=255)
                                                                                                                                                                                                                          ccGGCCTGCGCTCTGACATGGTCACTCTTGGTCAAAGCGCACTGCATTTCTCATCCGGGTCGCTTGATTGGATGAGCCATCTGCCAGATGGTCAACAGGTGACAGACATTCACAGTGGACGGGAATCTTTGCGGCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTgcg                                         <  2:90880/288‑1 (MQ=255)
                                                                                                                                                                                                                           cGGCCTGGCGTGGGCCGTTGTCATCCGGGGTCAAAGCGCACTAGATTTCTCATCCGGATCGCGGGATTTGCTGGGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTgcgc                                        <  2:979513/289‑1 (MQ=255)
                                                                                                                                                                                                                             gCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGGGGATTGGAATCTTTGCGTCAGCAGGCACCACCCCATCCTTACTCCCTTCTCGAGCCACTACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGCCATGAAACGGATAAAGCGGTGCGTACATGCAGGGCAGCGTAGAACTTGACCACCCTCGGGTTAGGCCGTCCGGTCATCTgcgc                                        >  2:229749/1‑287 (MQ=255)
                                                                                                                                                                                                                                     tgtgCCATTGGTCCTCTTGGTCAAAGCGAACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTgg                               <  2:1835841/288‑1 (MQ=255)
                                                                                                                                                                                                                                       tgCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCCCTACCTGGCTa                            >  1:743775/1‑289 (MQ=255)
                                                                                                                                                                                                                                         ccATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGTTCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAg                          <  2:745963/289‑1 (MQ=255)
                                                                                                                                                                                                                                               tcccTCGTGGTCAACGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGTACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGttcgcc                    <  2:623578/286‑6 (MQ=255)
                                                                                                                                                                                                                                               tCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGttcgcc                    >  1:765638/1‑284 (MQ=255)
                                                                                                                                                                                                                                                 aCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGttcgccag                  >  2:1801516/1‑282 (MQ=255)
                                                                                                                                                                                                                                                 aCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCCCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGACAGGAAGCGTAGAACTTGCTCCCCAGCGGGTTACGCCGCCCGGACCTCCGGGCTCTACCTGGACAAGtttcgcag                  >  2:45968/1‑282 (MQ=255)
                                                                                                                                                                                                                                                    cGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAg               >  1:1218609/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         <  2:1360724/213‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         >  1:1360724/1‑213 (MQ=255)
                                                                                                                                                                                                                                                               cgcACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTg     >  2:1484724/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                 cACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcg                                                          <  1:1002840/233‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGt        >  1:1574882/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                 cACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGcc  >  1:1136913/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  aCTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                         >  1:1166074/1‑201 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                           
TCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCAGTTCCGGAAGCCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC‑GCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTAGCCCAGAAGTCCCCGTCTGGCC  >  CP042583/1925376‑1925921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A