Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,948,758 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,948,7580AT99.2% 283.9 / NA 124intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (80/42);  total (81/43)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,948,7590CG97.6% 280.5 / NA 124intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (79/42);  total (81/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,948,7600TA99.2% 286.7 / NA 124intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (80/43);  total (81/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 124 total aligned reads displayed.

ATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑GCTGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCGCTACCACCGTGACCTGGGAGATCAACATACAACCGACTGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCG  >  CP042583/1948490‑1949043
                                                                                                                                                                                                                                                                             |||                                                                                                                                                                                                                                                                                           
aTTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCTTGACCTGGGAGCTCAATATACCACCGTGAGTAAGCGACAAACGccc                                                                                                                                                                                                                                                                            >  2:1761198/1‑287 (MQ=255)
     cGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCCCCAGCCAGAAGTCAAGGATGTTGTAACTAAACAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCCCCGCCGAACCACCGTTACCTGGGAGAACAACATACAACCGTGAGTACTCGGCAACcgccccgccc                                                                                                                                                                                                                                                                       >  2:978019/1‑288 (MQ=255)
       aTCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAG‑CCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCAACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCAct                                                                                                                                                                                                                                                                    >  1:1216571/1‑288 (MQ=255)
            aaCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtctt                                                                                                                                                                                                                                                                >  1:260241/1‑288 (MQ=255)
                   aCGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCg                                                                                                                                                                                                                                                        >  1:1081991/1‑289 (MQ=255)
                   aCGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGACCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGCAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCg                                                                                                                                                                                                                                                        >  2:220736/1‑289 (MQ=255)
                                          tttGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGt                                                                                                                                                                                                                                  >  1:27920/1‑288 (MQ=255)
                                          tttGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGCTGCCATCGCCGGAAAAACCGt                                                                                                                                                                                                                                  >  2:604325/1‑288 (MQ=255)
                                                 ccacgctaccccGCGTTAGACACTAACCCCACAAAGCCGCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCGGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAAGCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGG‑AAAACCGTGCAAAAaca                                                                                                                                                                                                                         <  2:1909670/280‑1 (MQ=255)
                                                  cccGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAaca                                                                                                                                                                                                                         <  1:993844/289‑1 (MQ=255)
                                                  cccGGATGCCCGCCTCAGACAATAACCCCACAGAGCCCCCCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAaca                                                                                                                                                                                                                         <  2:300200/289‑1 (MQ=255)
                                                      gATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcca                                                                                                                                                                                                                      >  2:158564/1‑288 (MQ=255)
                                                        tGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcc                                                                                                                                                                                                                   <  1:429939/289‑1 (MQ=255)
                                                        tGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                                                                                                                                    <  1:175758/288‑1 (MQ=255)
                                                                          aCCCCACAAAGCCCCCCCAGCCCCCGGAAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTc                                                                                                                                                                                                 <  2:27920/289‑1 (MQ=255)
                                                                                caAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGtt                                                                                                                                                                                           >  2:86632/1‑289 (MQ=255)
                                                                                     acccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGcc                                                                                                                                                                                      <  1:220736/288‑1 (MQ=255)
                                                                                      cccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTg                                                                                                                                                                                        >  1:311834/1‑286 (MQ=255)
                                                                                      cccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGcc                                                                                                                                                                                      >  2:721977/1‑288 (MQ=255)
                                                                                        ccccAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCCAGCGATG‑ACCCCACCAGACAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTgc                                                                                                                                                                                   <  2:139662/287‑1 (MQ=255)
                                                                                         gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGCGTAAACCTGGTTTTCCGCGTTTTTCCTgc                                                                                                                                                                                   >  2:1890159/1‑288 (MQ=255)
                                                                                              ccccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCACACGCCCCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAACACCACAGCCATGTTACAACGGGCTTTCCGTGTTTTCCCTGCGCGtc                                                                                                                                                                              >  2:1329952/1‑287 (MQ=255)
                                                                                              ccccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGc                                                                                                                                                                             <  1:721977/289‑1 (MQ=255)
                                                                                               cccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCa                                                                                                                                                                            <  1:1253643/289‑1 (MQ=255)
                                                                                                  tGGCAAGCCGCCATCCTCGCCACCCGTCCACCAAT‑CGAGTACCCCACCAGCCAGAAGGCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGa                                                                                                                                                                         <  2:629105/289‑1 (MQ=255)
                                                                                                  tGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAACCGCCGGAAAAACCGTGCAAAAACACCAGCCAACGTAAACCTGGTTTTCCAGGTTTTCCCCGCGCCTGCAggt                                                                                                                                                                         >  2:30949/1‑288 (MQ=255)
                                                                                                        gccgccATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaa                                                                                                                                                                   >  1:912510/1‑289 (MQ=255)
                                                                                                         ccgccATCATCGCCACCCGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATc                                                                                                                                                                                                                                                >  2:500008/1‑211 (MQ=255)
                                                                                                                         ccGTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCa                                                                                                                                                  >  1:1878039/1‑289 (MQ=255)
                                                                                                                           gTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                                                                                                                    >  1:300726/1‑269 (MQ=255)
                                                                                                                           gTCCACCAAG‑CGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGACGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAACCGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCAGGAAAAACCGTGCAAAAACCCCAGCCAGGGTAAAACTGGTTTTCCGGTATTTGCCTGACCGTTCAGGATCATAAATGGCTTACCTgcgcagga                                                                                                                                                >  2:57348/1‑285 (MQ=255)
                                                                                                                                 ccaagcgagTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGtt                                                                                                                                            >  2:946853/9‑288 (MQ=255)
                                                                                                                                  catgcgactACCCCACCAGCCAAAAGTCAAGGGTGTTGTAACTAACCAAGGTGTTACTCAGTAAGTCGGTGACATCATCAAATCCATGTACGATAATCGCGGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGc                                                                                                                                          <  2:682790/281‑1 (MQ=255)
                                                                                                                                    agcgagTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTg                                                                                                                                        >  1:1656194/6‑289 (MQ=255)
                                                                                                                                    agcgagTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTg                                                                                                                                        >  1:164635/6‑289 (MQ=255)
                                                                                                                                       gagTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                                                                                                                                     <  1:604325/287‑1 (MQ=255)
                                                                                                                                        agTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGG‑AAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCg                                                                                                                                   >  2:1774733/2‑289 (MQ=255)
                                                                                                                                          tACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGt                                                                                                                                  >  1:1415751/1‑289 (MQ=255)
                                                                                                                                            ccccACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGTAGTTGCTGGAGCGttt                                                                                                                                >  1:257826/1‑289 (MQ=255)
                                                                                                                                            ccccACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGttt                                                                                                                                >  1:1092299/1‑289 (MQ=255)
                                                                                                                                               caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                                                                                                                                    <  1:1031257/202‑1 (MQ=255)
                                                                                                                                               caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagc                                                                                                                                                                                                                    >  2:1031257/1‑202 (MQ=255)
                                                                                                                                               caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgcg                                                                                                                             <  1:1890159/289‑1 (MQ=255)
                                                                                                                                               caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTgc                                                                                                                              >  1:460142/1‑288 (MQ=255)
                                                                                                                                                    gccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGTAACACCCGTGCAAACACACCCGCCAGCGTAAACCTGGTTTTCCGTGTTTTGCCCGCCTCCGCTGGATCCGAAATGGCTACACTGCTCCAGTCGTTGCGTGAGCGTGTTCTCAcc                                                                                                                         >  2:1416878/1‑288 (MQ=255)
                                                                                                                                                    gccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTc                                                                                                                                                                                                 >  1:978764/1‑216 (MQ=255)
                                                                                                                                                             cAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGgtgt                                                                                                                 <  2:164635/287‑1 (MQ=255)
                                                                                                                                                              aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGt                                                                                                              >  1:707972/1‑289 (MQ=255)
                                                                                                                                                              aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGAGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTGGCGCACCATCGGTGTGGt                                                                                                              >  1:814322/1‑289 (MQ=255)
                                                                                                                                                               aGGATGTTGTAACTAACCAAGGTTTTACGCACTAAGTCGTTGCCAACATCAAATCCCTCGACGCTAAACGCCGCCGAACCACCGTGACCTGCGAGATCAACATACCAACGCCTCTACGCGGCAAAAGCCTCGCCCCCTTCTTCCCATTTGTGGCAATCGCCGGAAAACCCTTGTAAAAACAGCAGCCAGGCTAAACCTGGTTATCCTTATGTTGCCTGCGCGTGCACGATCATCAGTGGCTTACCTTAACCAGAATTTGCTGCAGCGTATGCGCACTATCGgtgcctt                                                                                                              >  2:283236/1‑284 (MQ=255)
                                                                                                                                                                      tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCCAAAACACCCGCCACGGTAAACCTCGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCGGCGCCAGTAGTTGCTGGAGCGTTTGCGCACCATCGGTTGCCTTAaccac                                                                                                       >  2:1184206/1‑288 (MQ=255)
                                                                                                                                                                      tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAaccacc                                                                                                      >  1:775351/1‑289 (MQ=255)
                                                                                                                                                                      tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAaccacc                                                                                                      >  2:967213/1‑289 (MQ=255)
                                                                                                                                                                           ctaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTc                                                                                                 >  1:1635325/1‑289 (MQ=255)
                                                                                                                                                                              acCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                                                                                                                                     >  2:1676637/1‑250 (MQ=255)
                                                                                                                                                                              acCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAg                                                                                                                                     <  1:1676637/250‑1 (MQ=255)
                                                                                                                                                                               ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCTCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAATTTGCTGGAGCGTCTGCGCACGATCGGTGTCCTTAACCACTATTTCAATc                                                                                             >  2:1107899/1‑289 (MQ=255)
                                                                                                                                                                               ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAt                                                                                                                                                                        <  2:1655935/214‑1 (MQ=255)
                                                                                                                                                                               ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAt                                                                                                                                                                        >  1:1655935/1‑214 (MQ=255)
                                                                                                                                                                               ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATc                                                                                             >  1:311040/1‑289 (MQ=255)
                                                                                                                                                                               ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATc                                                                                             >  1:433106/1‑289 (MQ=255)
                                                                                                                                                                               ccAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAGCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGGCCACCATCGGTGTCGGTAACCACCATTTCAAt                                                                                              >  2:933659/1‑288 (MQ=255)
                                                                                                                                                                                cAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATc                                                                                             >  1:1238524/1‑288 (MQ=255)
                                                                                                                                                                                cAAGGTTTTACGCAGTAAGTCGGGGACATCAGCAAATCCATCGCCGCTAATCGCCGCCGAACCACCGGGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCa                                                                                            <  2:1081991/289‑1 (MQ=255)
                                                                                                                                                                                     ttttACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCgt                                                                                        <  2:912510/288‑1 (MQ=255)
                                                                                                                                                                                                aaGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGc                                                                              <  1:158564/287‑1 (MQ=255)
                                                                                                                                                                                                       tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt                                                                                                             <  1:1574951/249‑1 (MQ=255)
                                                                                                                                                                                                       tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGtt                                                                                                             >  2:1574951/1‑249 (MQ=255)
                                                                                                                                                                                                       tGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAg                                                                     >  1:1384890/1‑289 (MQ=255)
                                                                                                                                                                                                         aCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGc                                                                   >  1:75810/1‑289 (MQ=255)
                                                                                                                                                                                                           atcatcAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACCACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGACGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCCCGTGCAGGCTCTTAACTGTCTTACCCGCGCCAGAAGTTGCAGGAGCGTTTGCTCACCATCGGTGTTGTTAACCACGATTTAAATCACTGGGGGGGTTCGCGTGCGCCGGGCg                                                                  >  2:1075994/1‑288 (MQ=255)
                                                                                                                                                                                                           atcatcAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCg                                                                  >  1:983923/1‑288 (MQ=255)
                                                                                                                                                                                                           atcatcAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGt                                                                 <  1:1761198/289‑1 (MQ=255)
                                                                                                                                                                                                                      cATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGcc                                                      >  1:1393682/1‑289 (MQ=255)
                                                                                                                                                                                                                       aTCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGG‑TTTCCGTGTTTTGCCTGCGCGTGCAGGATGATAAATGGCTTACCTGCCCCCGCAGTTGCTGGACCGTTTGTGCACCAACGGTGTCGGTACTCTCCATTTCAATCACCGTGGTTGTTGGCGTTCGACAGGCGTCAGCaacgtctgt                                                    >  2:872616/1‑282 (MQ=255)
                                                                                                                                                                                                                             gCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                                                                                                                    >  1:926378/1‑172 (MQ=255)
                                                                                                                                                                                                                              cTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAAt                                                                                              >  2:1660292/1‑241 (MQ=255)
                                                                                                                                                                                                                              cTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAAt                                                                                              <  1:1660292/241‑1 (MQ=255)
                                                                                                                                                                                                                              cTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAg                                              >  2:1616159/1‑289 (MQ=255)
                                                                                                                                                                                                                                      cgccgaaccaccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGcc                                      >  1:676758/7‑289 (MQ=255)
                                                                                                                                                                                                                                           aaccaccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCAACATTTCAATCACCCGTGTGGGTGGCGTGCGCCAGGGCTCGGGCACTCCCGTTCCAACTTTCTGcccggtg                                 >  2:1261468/2‑284 (MQ=255)
                                                                                                                                                                                                                                               accGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCg                             <  1:1124360/289‑1 (MQ=255)
                                                                                                                                                                                                                                                ccGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGGTGTGGTTGGCGTTCGCCAGGCGTCGGCAAATCCCGTTTCAAGTTCCTGCCAGTTTTGCgg                            >  2:1051299/1‑289 (MQ=255)
                                                                                                                                                                                                                                                  gagACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGAc                          <  2:664171/287‑1 (MQ=255)
                                                                                                                                                                                                                                                   tGACCTGGGAGATCAACATAGAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGGGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGAc                          <  1:985084/288‑1 (MQ=255)
                                                                                                                                                                                                                                                   tGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACg                         >  1:892024/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      ccTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACgatg                      <  2:676758/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGG‑AAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGata                   >  1:453299/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          ggAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGAGCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCACCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCTTTTCACGTTCCTGCCAGTTTTGCGGACGATGatat                  >  2:1235682/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           gagaTCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATAtt                 >  1:998553/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGCAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCCCGTGCACGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGCAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGCTGGTTGGCTGTCGCCAGTCGTCGGCAAATCCCGTGTCACGTTCCTCCCAGGTTTTCCGTCGCTGATAttcta              >  2:1497425/1‑285 (MQ=255)
                                                                                                                                                                                                                                                               tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCa              >  1:1317800/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               tCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATAGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGGGGTGGTTGGCGCGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTTCGGACGATGAAAtttga              >  1:1356407/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATa                                                                                                                                                                    <  2:340213/137‑1 (MQ=255)
                                                                                                                                                                                                                                                                cAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCa              >  1:317753/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                 aaCATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGa                                                                                                                                                <  1:327744/156‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTCCCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCCTCCGCAAATGCCGTTGCAAGTGCCTGCCCGTTTTGCGGACGATGATGTTTAAGCTCg         >  2:1550629/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     tACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGa        >  1:636549/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                     tACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGa        >  1:1140324/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                          ccGACAGTCGTCGGACAACGCCTCGACCACTTTTTGCCAGTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATcg  <  2:1656194/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             |||                                                                                                                                                                                                                                                                                           
ATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAG‑GCTGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCGCTACCACCGTGACCTGGGAGATCAACATACAACCGACTGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCG  >  CP042583/1948490‑1949043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A