Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,953,760 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,953,7600AT99.1% 524.5 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (112/106);  minor base G (2/0);  total (115/107)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,953,7610GC99.1% 512.0 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (113/105);  minor base A (2/0);  total (115/107)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,953,7620TA98.7% 516.4 / ‑4.3 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (114/105);  minor base C (1/2);  total (115/107)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

ATTACTTAGCTGCTCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCAGCCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGAGTATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTC  >  CP042583/1953482‑1954040
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
aTTACTTAGCTGCTCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTCATCATTACCCACGTCAGCTCGCGCTGTGATGACAAAGGTGGGCAGCACCTGTTCCGTGAATGCAGGTCAATTTTccc                                                                                                                                                                                                                                                                                >  2:1159352/1‑289 (MQ=255)
     gtacctggtCCAGTGCCATGGAAAGCGCTCGTTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGTTATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGa                                                                                                                                                                                                                                                                           <  2:1901253/285‑1 (MQ=255)
            ctCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGa                                                                                                                                                                                                                                                                       >  1:836217/1‑286 (MQ=255)
            ctCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGa                                                                                                                                                                                                                                                                       >  1:1433259/1‑286 (MQ=255)
            ctCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGa                                                                                                                                                                                                                                                                       >  2:744406/1‑286 (MQ=255)
            ctCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGGGACTGCAGGTAAATTTTCCCGGCCACTGa                                                                                                                                                                                                                                                                       >  2:1199646/1‑286 (MQ=255)
              ccAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAAGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTg                                                                                                                                                                                                                                                                   >  1:1385983/1‑288 (MQ=255)
                actGCCAGGTAAAGCGCCCGCTATTTCAGGCGATACCGGCCCCTGGGAGGCCGCCATTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCg                                                                                                                                                                                                                                                                <  2:1458125/287‑1 (MQ=255)
                   gacgggTAAAGCGCCGGCGATGTGCGGCGATACCGGCCCCGGCGAGGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGaa                                                                                                                                                                                                                                                              <  2:295731/284‑1 (MQ=255)
                   gCCAGGTAAGGCGCTCGCTATTTTCGCCGATACCGGCCCCTGCGATGCCGAACGTGACCTGGCTAAAGGTGTCGATGTCATGGGGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCAAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCg                                                                                                                                                                                                                                                                <  2:873877/286‑1 (MQ=255)
                       ggTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGCCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTCCGAGAATGCAGGTCAATTTTCCCGGCCACTGAACTGGCGAATGAtt                                                                                                                                                                                                                                                         >  2:1822693/1‑289 (MQ=255)
                       ggTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTAGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACCCTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCCTTACCCACGTCAGCTCGCGCTATGATGACAACGGTTGTCAGAACATGTTACGTGAATGCAGGTCAAATTTCCCGGCGACTGAACTGGCGAATGAtt                                                                                                                                                                                                                                                         >  2:1368464/1‑289 (MQ=255)
                            aGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCg                                                                                                                                                                                                                                                                <  2:1660393/277‑1 (MQ=255)
                            aGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCAc                                                                                                                                                                                                                                                     >  2:778410/1‑288 (MQ=255)
                            aGCGCGCGCTATTTTCGGCGATACCGGCCCCTGCGCTGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCCCTACACGCCAGGCTGCGACACTAGCCCGTGACGCCCGACTCGGCAAGCTAATCCTTACCCACTTCATCTCGCGCTATTATGACCAAGGTCGTCAGCCCCTGTCACGTGCATGCACGCAAATGTTCCCGGCCACGAAACGTGCGAATGATTTCAcc                                                                                                                                                                                                                                                    >  2:1688581/1‑289 (MQ=255)
                                 tcgctATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGGCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCCTgtt                                                                                                                                                                                                                                               >  2:1571892/1‑289 (MQ=255)
                                   gtctttttCGGGGCTACCGGCCCCTGCGATGCCGAACTTGCCCTGGCTAAAAGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTgttta                                                                                                                                                                                                                                             <  2:1385983/285‑1 (MQ=255)
                                   ggtATTTTGGGCGGTACAGGCCCCGGCGATGCCGGACTTGACCTGGCTAAAGGGGTCGATGTCATGGTGAACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTgttta                                                                                                                                                                                                                                             <  2:1913713/287‑1 (MQ=255)
                                       ttttCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTgtttaacgt                                                                                                                                                                                                                                         >  1:5163/1‑289 (MQ=255)
                                                 tACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAActct                                                                                                                                                                                                                                 >  2:946852/1‑287 (MQ=255)
                                                    cGGCCCCTGGGATGCCGCACTTGACCTGGGTAAGGGGTTAGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCtta                                                                                                                                                                                                                            <  2:1425066/289‑1 (MQ=255)
                                                    cGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCtta                                                                                                                                                                                                                            <  2:177567/289‑1 (MQ=255)
                                                              gATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTaaaaa                                                                                                                                                                                                                  <  1:94880/289‑1 (MQ=255)
                                                                 gCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGa                                                                                                                                                                                                                                                                           <  2:1142266/229‑1 (MQ=255)
                                                                 gCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGa                                                                                                                                                                                                                                                                           >  1:1142266/1‑229 (MQ=255)
                                                                 gCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACa                                                                                                                                                                                                                <  2:105505/288‑1 (MQ=255)
                                                                    caaCTTGAACTGTGTAAAGGTGTAGATGTCAGGGTGCACGAAGCGACGCTGGATATACACATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGc                                                                                                                                                                                                            <  2:498825/287‑1 (MQ=255)
                                                                          gACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACt                                                                                                                                                                                                      >  1:1485544/1‑289 (MQ=255)
                                                                          gACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGCTAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACTGTTAACTCTGCTTATTCTTACAAACATGGCGAAACt                                                                                                                                                                                                      >  2:1224506/1‑289 (MQ=255)
                                                                            ccTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTg                                                                                                                                                                                                    <  1:986973/289‑1 (MQ=255)
                                                                                     aGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACCGGGCGAAACTTGCCCTGTTa                                                                                                                                                                                            >  1:1187709/1‑288 (MQ=255)
                                                                                          tCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGCCAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCCATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTTTTAAAAACAGGGCGAAACTTGCCCTGTTATCGCa                                                                                                                                                                                       >  1:347398/1‑288 (MQ=255)
                                                                                          tCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCa                                                                                                                                                                                       >  1:1659654/1‑288 (MQ=255)
                                                                                           cGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAAc                                                                                                                                                                                     <  1:66469/289‑1 (MQ=255)
                                                                                                 gaTGGTGCAGGACGCGACGCTGGATATACCCAGGGAAGACAAGGCCAATAGTAGCGGCCATAGCTCTACACGCCAGGCTGCGCCACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCcgcg                                                                                                                                                                                <  2:44011/287‑1 (MQ=255)
                                                                                                 cATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAGCGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCcgcg                                                                                                                                                                                <  1:1057848/288‑1 (MQ=255)
                                                                                                 cATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCcgcg                                                                                                                                                                                <  1:581561/288‑1 (MQ=255)
                                                                                                 cATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCgcgc                                                                                                                                                                               <  1:1312563/289‑1 (MQ=255)
                                                                                                          cGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTgtt                                                                                                                                                                                                                                               >  2:1064047/1‑216 (MQ=255)
                                                                                                                gACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATccac                                                                                                                                                                  <  1:778410/287‑1 (MQ=255)
                                                                                                                  cGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATccaccca                                                                                                                                                               <  1:1664478/288‑1 (MQ=255)
                                                                                                                   gCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCcaca                                                                                                                                                             <  1:1640420/289‑1 (MQ=255)
                                                                                                                              ccATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTg                                                                                                                                                  >  1:1028113/1‑289 (MQ=255)
                                                                                                                                 tGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCtta                                                                                                                                                                                                                            <  2:770319/212‑1 (MQ=255)
                                                                                                                                 tGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTGTTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGc                                                                                                                                               >  1:1887222/1‑289 (MQ=255)
                                                                                                                                 tGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCgcgc                                                                                                                                                                               >  1:107836/1‑257 (MQ=255)
                                                                                                                                 tGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGc                                                                                                                                               >  2:1059070/1‑289 (MQ=255)
                                                                                                                                 tGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTATGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGc                                                                                                                                               >  1:1436404/1‑289 (MQ=255)
                                                                                                                                         aaagccaaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTgttta                                                                                                                                                                                                                                             <  2:900546/187‑1 (MQ=255)
                                                                                                                                         aaagccaaTAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTgttta                                                                                                                                                                                                                                             >  1:900546/1‑187 (MQ=255)
                                                                                                                                           agccaaTCGGAGCGGCCATAGGTCTAAACGCCAAGCTGCGAAACTAGCCCGTGAGGCGGGCGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTCGCAGCACCTGTTACGGGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATAGCCACCCACACAGGCATATTGAGCGATTTTTGat                                                                                                                                     <  2:824378/289‑1 (MQ=255)
                                                                                                                                                 tAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATACACCCACACAGGCATATTGAGCGATTTTTGAtatttat                                                                                                                               >  1:845010/1‑289 (MQ=255)
                                                                                                                                                      gcgGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCa                                                                                                                            >  2:1499687/1‑287 (MQ=255)
                                                                                                                                                      gcgGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCAtc                                                                                                                          <  1:722372/289‑1 (MQ=255)
                                                                                                                                                      gcgGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCAtc                                                                                                                          <  1:322793/289‑1 (MQ=255)
                                                                                                                                                       cgGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTCAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCAtc                                                                                                                          >  2:201748/1‑288 (MQ=255)
                                                                                                                                                          ccATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTcc                                                                                                                       <  1:1580938/288‑1 (MQ=255)
                                                                                                                                                           cATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCtt                                                                                                                     >  1:1000298/1‑289 (MQ=255)
                                                                                                                                                             tAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTAc                                                                                                                   <  1:1499687/289‑1 (MQ=255)
                                                                                                                                                             tAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTAc                                                                                                                   <  1:1159352/289‑1 (MQ=255)
                                                                                                                                                             tAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCAGATTGAGCGATTTTTGATATTTATTCATCTCCTTAc                                                                                                                   >  1:1573087/1‑289 (MQ=255)
                                                                                                                                                              aGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTAc                                                                                                                   >  2:649446/1‑288 (MQ=255)
                                                                                                                                                                 tctACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCa                                                                                                                <  2:420105/288‑1 (MQ=255)
                                                                                                                                                                  ctACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTAATATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACt                                                                                                              <  1:183791/289‑1 (MQ=255)
                                                                                                                                                                    acacGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCAACTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACACGCGCTACATACATCCACCAACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACtat                                                                                                            >  1:1270541/1‑289 (MQ=255)
                                                                                                                                                                         ccAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCa                                                                                                       <  1:1468397/289‑1 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGcc                                                                                                                                                                                                  <  1:921349/197‑1 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGcc                                                                                                                                                                                                  >  2:921349/1‑197 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCag                                                                                                      >  1:536343/1‑289 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCag                                                                                                      >  1:1332398/1‑289 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCa                                                                                                       <  2:786456/288‑1 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCa                                                                                                       <  2:1358682/288‑1 (MQ=255)
                                                                                                                                                                          cAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTACTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCag                                                                                                      >  2:222503/1‑289 (MQ=255)
                                                                                                                                                                             gCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCaagac                                                                                                   >  2:132787/1‑285 (MQ=255)
                                                                                                                                                                               tGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGAATGAACTGGCGAATGATTGCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTCCCCTGTTATCGCCAACCGCCCTACATATATCCACCCAACCAGGCAGATTGAGCGAATTTAGATATTTATTCATCTCCTTACGCACTATATTCagaaaaa                                                                                                 >  2:253130/1‑285 (MQ=255)
                                                                                                                                                                                      cTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCa                                                                                                                >  2:1626168/1‑267 (MQ=255)
                                                                                                                                                                                                   cTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCt                                                                             >  1:174574/1‑289 (MQ=255)
                                                                                                                                                                                                           ggCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTc                                                                                                                                                                                                                                                       <  1:404915/111‑1 (MQ=255)
                                                                                                                                                                                                            gCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCa                                                                                                       >  1:449157/1‑254 (MQ=255)
                                                                                                                                                                                                            gCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCa                                                                                                       <  2:449157/254‑1 (MQ=255)
                                                                                                                                                                                                            gCAAGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGCCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGc                                                                    >  2:351824/1‑289 (MQ=255)
                                                                                                                                                                                                               aGCTAATCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGcaca                                                                 <  1:194706/289‑1 (MQ=255)
                                                                                                                                                                                                                     tCATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCttt                                                           >  2:327303/1‑289 (MQ=255)
                                                                                                                                                                                                                      cATTACCCACGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTg                                                          <  2:561681/289‑1 (MQ=255)
                                                                                                                                                                                                                              aCGTCAGCTCGCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTaaa                                                  >  2:628733/1‑289 (MQ=255)
                                                                                                                                                                                                                                gtcagctcgcGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAg                                                >  2:1493572/10‑289 (MQ=255)
                                                                                                                                                                                                                                gtcagctcgcGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAg                                                <  1:1688581/280‑1 (MQ=255)
                                                                                                                                                                                                                                     ctcgcGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCa                                                                                                                >  1:617254/5‑220 (MQ=255)
                                                                                                                                                                                                                                          gCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTg                                                          <  1:232076/269‑1 (MQ=255)
                                                                                                                                                                                                                                          gCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCatga                                      >  2:1086802/1‑289 (MQ=255)
                                                                                                                                                                                                                                          gCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCatg                                       <  1:819025/288‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaGGTTGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTAAAGTTg                          >  2:707738/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        ggTTGTCAGCACCTGTTACGTGTATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGc                                                                    >  1:178629/1‑245 (MQ=255)
                                                                                                                                                                                                                                                          ttGTCAGCACCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCa                       >  2:3687/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                  aCCTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAattatt              <  2:405897/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccTGTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTg             >  1:692963/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      gTTACGTGAATGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCa          >  1:1120421/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              aaTGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTAtctc  >  1:745754/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              aaTGCAGGTCAATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTAtctc  >  1:810258/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                               aTGCAGGTGCATTTTACCGGCGACTTAACTGGCGAATGATTTCACCGGGTTTAACGTTTAACTCGGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTAtctc  <  2:1035560/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
ATTACTTAGCTGCTCCAGTGCCAGGTAAAGCGCTCGCTATTTTCGGCGATACCGGCCCCTGCGATGCCGCACTTGACCTGGCTAAAGGTGTCGATGTCATGGTGCACGAAGCGACGCTGGATATAACCATGGAAGCCAAAGCCAATAGTCGCGGCCATAGCTCTACACGCCAGGCTGCGACACTAGCCCGTGAGGCTGGAGTCGGCAAGCTAATCATTACCCACGTCAGCAGCCGCTATGATGACAAAGGTTGTCAGCACCTGTTACGTGAATGCAGGAGTATTTTCCCGGCGACTGAACTGGCGAATGATTTCACCGTGTTTAACGTTTAACTCTGCTTATTATTAAAAACAGGGCGAAACTTGCCCTGTTATCGCAACCCGCGCTACATATATCCACCCACACAGGCATATTGAGCGATTTTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTCATCTTGATTCAGCACAACCTTTGCAACTAAAAGGAGCCCATGATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTC  >  CP042583/1953482‑1954040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A