Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,976,349 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,976,3490AT99.5% 509.8 / NA 215intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (93/121);  minor base G (1/0);  total (94/121)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,976,3500CG98.6% 497.8 / NA 215intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (91/120);  minor base T (2/1);  total (94/121)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,976,3510TA100.0% 514.0 / NA 215intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (94/121);  total (94/121)

Only 100 of 218 total aligned reads displayed.

CCCAGCCGCTTTCGCGGATAGAGGTACTCCATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CGCTGTCGATACCGGATTCAAACGGCACGTTTTTGCTCCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTACTCATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATG  >  CP042583/1976069‑1976625
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                  
cccAGCCGCTTTCGGGGAGAGAGGTAGACCATGCGAACAGATACAGCGCTTCAACGTAGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGttgacatact                                                                                                                                                                                                                                                                              <  2:1061962/289‑1 (MQ=255)
cccAGCCGCTTTCGCGGATAGAGGTTGACCATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGttgacatact                                                                                                                                                                                                                                                                              <  1:472029/289‑1 (MQ=255)
 cccgcagcgttCGCGGAAAGAGGTTGACCATGCGAACAGATAAAGCGCTTCAAAGTCGAAGATAACGAAGAACATGGACACCAGATAAAA‑CGTGGCGGAAAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGttgacatactc                                                                                                                                                                                                                                                                             <  2:1386537/280‑1 (MQ=255)
      cGCTTTCGCGGATAGAGGTTGACCATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCAtt                                                                                                                                                                                                                                                                          >  1:526957/1‑287 (MQ=255)
        cTTTCGCGGATAGAGGTTGACCATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGAAAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTTGAAGTTAACATACTCATTGCtt                                                                                                                                                                                                                                                                      >  2:373442/1‑289 (MQ=255)
                       gttgaccATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGAGGTTGACATACTCATTGCTTACTAATCAAAAGTa                                                                                                                                                                                                                                                        >  1:399545/6‑288 (MQ=255)
                          gaccATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAgcgc                                                                                                                                                                                                                                                    <  1:1742949/287‑1 (MQ=255)
                           accATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCCACACAGGCCAATGGCAACGAAAAGAAAGATAGCGAATGACCAGTCATGAGCGATTACTTCAGTGGATTTTGACATACTAATTGCTTACTCATCAAAAGTATCGcc                                                                                                                                                                                                                                                   >  2:830055/2‑289 (MQ=255)
                           accATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTTACATACTCATTTCTTACTCATCAAAAGTAgcgc                                                                                                                                                                                                                                                    >  1:908889/2‑288 (MQ=255)
                                        aTACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGc                                                                                                                                                                                                                                        >  2:1389870/1‑287 (MQ=255)
                                          aCAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGctct                                                                                                                                                                                                                                     >  1:1368146/1‑288 (MQ=255)
                                          aCAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGctct                                                                                                                                                                                                                                     >  1:32895/1‑288 (MQ=255)
                                          aCAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGAAGAGCGATGACTTCAGTGGATGTTTACATACTCATTGCTTACTCATTAAAAGTAGACCCAGATTCACTTCTCtt                                                                                                                                                                                                                                    >  2:499689/1‑289 (MQ=255)
                                             gcgcTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCAc                                                                                                                                                                                                                                 >  2:431634/1‑289 (MQ=255)
                                              cgcTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACg                                                                                                                                                                                                                                >  2:1290452/1‑289 (MQ=255)
                                              cgcTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACg                                                                                                                                                                                                                                >  2:1168306/1‑289 (MQ=255)
                                              cgcTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACg                                                                                                                                                                                                                                >  2:541307/1‑289 (MQ=255)
                                               gcTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCa                                                                                                                                                                                                                                  <  1:1440277/286‑1 (MQ=255)
                                               gcTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCAc                                                                                                                                                                                                                                 <  1:1008545/287‑1 (MQ=255)
                                               cgctCAACGTCGAAGATAAAGAAGAACATGACCACCAGATAAAA‑CATGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CGGAGTCGCTACCGGATTCAAACGGCACGTTTTTGAACCTCGCGCGTGCGCGCCCGCATAAAACCCAGCCGCCGACCAGCATCAGGAAACACAGACCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCGCTTCACgg                                                                                                                                                                                                                               <  2:330195/286‑1 (MQ=255)
                                                cTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGc                                                                                                                                                                                                                              >  1:1332813/1‑289 (MQ=255)
                                                  tCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGCTTCACTGCTCTTCACGGCAg                                                                                                                                                                                                                            >  1:181580/1‑289 (MQ=255)
                                                     aagTCGAAAGTAGCCAAAAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGGGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATg                                                                                                                                                                                                                         <  2:25457/287‑1 (MQ=255)
                                                               tAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCcaca                                                                                                                                                                                                                <  2:1803620/288‑1 (MQ=255)
                                                               tAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACAt                                                                                                                                                                                                               <  1:1875688/289‑1 (MQ=255)
                                                                         cATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTAATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATgg                                                                                                                                                                                                     >  1:96501/1‑289 (MQ=255)
                                                                          aagggacagggATAAAC‑CTTGGCGGACAGGGGGAAGCGGGCGCAGCAGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATggg                                                                                                                                                                                                    <  2:1837846/279‑1 (MQ=255)
                                                                                cacaGATAAAATCTTGGCGGCCAGGCGGAAGCGGGAGGAGCCGA‑CCGATTGGAGACCTGATTCAAACGTAACGTTGTGCGACCACGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACCGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATTACGTCAGTGGATGGTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGggagga                                                                                                                                                                                               <  2:30029/287‑1 (MQ=255)
                                                                                      tAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATaaaaa                                                                                                                                                                                        <  1:1290452/289‑1 (MQ=255)
                                                                                         atatttGGAGGACATGCGTAAGCGGCAGGAGCCGACCTATGTTG‑TGCCTAATTCAAACGGCACCTTATCAGACAGCGCGCAGTCGCGACAACCTAAACACAAACCGCCTACAATGCTCAAGCAACACGAGCCAATGGCAACGATAAGAAAGATAGCGCATGCCCAGTGGTGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCGTCAACAGTAGCGCCAGCTTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATaaaaaaa                                                                                                                                                                                      <  2:1114453/285‑1 (MQ=255)
                                                                                           acgtGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAc                                                                                                                                                                                    <  1:618089/286‑1 (MQ=255)
                                                                                               ggCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATaaa                                                                                                                                                                                          >  1:1837846/1‑279 (MQ=255)
                                                                                               ggCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTa                                                                                                                                                                                >  2:1817142/1‑289 (MQ=255)
                                                                                                gCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATc                                                                                                                                                                                                              >  1:282977/1‑258 (MQ=255)
                                                                                                gCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATc                                                                                                                                                                                                              <  2:282977/258‑1 (MQ=255)
                                                                                                gCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTAc                                                                                                                                                                               >  2:1223643/1‑289 (MQ=255)
                                                                                                gCGGACAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAAGTGGACGCCACAATGGTTCAGGGGGGGGGAAAAAAAAAAACTTaa                                                                                                                                                                               >  2:146559/1‑288 (MQ=255)
                                                                                                    aCAGGCGTGAGCGGCCGGAGCCGA‑CCCGGTCGATACCGGATTCAAACGGCACATTTTTCGGCCACGCGAGTGGGGAACCGCCTAAAAACCCACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAAt                                                                                                                                                                            <  2:674027/288‑1 (MQ=255)
                                                                                                    aCAGGCGTAAGCGGGCGGAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAAt                                                                                                                                                                            <  1:541307/288‑1 (MQ=255)
                                                                                                                  gcggAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACCTCCTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCTTGGGGCTGAATAAAAACCACCACACAATCACTGTAGACCTTCt                                                                                                                                                             >  2:41141/1‑289 (MQ=255)
                                                                                                                  gcggAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCcaca                                                                                                                                                                                                                >  2:1532335/1‑238 (MQ=255)
                                                                                                                  gcggAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAAt                                                                                                                                                                            <  1:972996/274‑1 (MQ=255)
                                                                                                                  gcggAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCt                                                                                                                                                             >  2:19365/1‑289 (MQ=255)
                                                                                                                  gcggAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATAACCATAGAAATTCt                                                                                                                                                             >  2:1357756/1‑289 (MQ=255)
                                                                                                                   cggAGCCGA‑CCGAGTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTGGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATTGACGCCACATCGATTCTTGGGTAGGAATAAAAAAAACCTTACAATAACTGTAGAAattatt                                                                                                                                                            >  2:686819/1‑289 (MQ=255)
                                                                                                                          cgaccgagTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCCTCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTtata                                                                                                                                                       <  1:1168306/281‑1 (MQ=255)
                                                                                                                          cgaccgagTCGATACCGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTtata                                                                                                                                                       <  1:146559/281‑1 (MQ=255)
                                                                                                                                        ccGGATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGAtgtg                                                                                                                                        <  2:324579/289‑1 (MQ=255)
                                                                                                                                        ccGAATTCAAACGGCACGTTTTTAGACCTCGCGCGTGCGCGAACGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACAAAGGCCAATGGCAACGATAAGAAAGATAGCGAATGACCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTACTTACTCATCAAAAGTAGCGCCAGATTAACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGAtgtg                                                                                                                                        <  2:225127/289‑1 (MQ=255)
                                                                                                                                           gATTCAAACGGCACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGtt                                                                                                                                     >  1:85404/1‑289 (MQ=255)
                                                                                                                                                    ggCACGTTTCTCGACCCCGCGGGTGCGCACCCGCCTAAAAACCAACCGCCTACCAGAATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                                                                            <  2:455753/289‑1 (MQ=255)
                                                                                                                                                     gCACGTTTTCGGACTCCGCGCGTGCGCGACCCACTAAAAACCAACCGCCTACCAGCATCAGGCAACACCAGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTcc                                                                                                                            <  2:1664616/288‑1 (MQ=255)
                                                                                                                                                      cACGTTTTTCGACCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCt                                                                                                                           <  1:833545/288‑1 (MQ=255)
                                                                                                                                                                     cgcgcgTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGt                                                                                                           >  1:784443/1‑289 (MQ=255)
                                                                                                                                                                     cgcgcgTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGt                                                                                                           >  2:483043/1‑289 (MQ=255)
                                                                                                                                                                          gTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAAc                                                                                                       <  1:587685/288‑1 (MQ=255)
                                                                                                                                                                                gACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGCATGCACAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACa                                                                                                <  2:641547/289‑1 (MQ=255)
                                                                                                                                                                                  ccGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACaaa                                                                                              >  1:1824247/1‑289 (MQ=255)
                                                                                                                                                                                          aaaCCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTttctt                                                                                      <  2:1332813/289‑1 (MQ=255)
                                                                                                                                                                                              caaccGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                                                                                  <  1:686819/289‑1 (MQ=255)
                                                                                                                                                                                              caaccGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                                                                                  <  1:1494084/289‑1 (MQ=255)
                                                                                                                                                                                              caaccGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                                                                                  <  1:167620/289‑1 (MQ=255)
                                                                                                                                                                                              caaccGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                                                                                  <  1:739173/289‑1 (MQ=255)
                                                                                                                                                                                              acaccGCCTACAAGCATCAGGGAACACAGGTCAATGGCAACGTTAAGAAAACGAGCGAATGGCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAACACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTcaca                                                                                  <  2:1590742/287‑1 (MQ=255)
                                                                                                                                                                                                accGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACAtt                                                                                >  1:487841/1‑289 (MQ=255)
                                                                                                                                                                                                accGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGACTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCGAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTAAACTTTAACAAAAGTTTCTTCACAtt                                                                                >  2:1516026/1‑289 (MQ=255)
                                                                                                                                                                                                   gCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGt                                                                             <  1:1738588/289‑1 (MQ=255)
                                                                                                                                                                                                         cAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACat                                                                       <  2:784443/289‑1 (MQ=255)
                                                                                                                                                                                                          agcacaacgCAAAACAGGCCAATGGCAACGATAATAAAGATAGCGAATGCACAGTGATGATCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGGAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACata                                                                      <  2:96501/281‑1 (MQ=255)
                                                                                                                                                                                                               cAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATc                                                                 <  1:1658889/289‑1 (MQ=255)
                                                                                                                                                                                                                 ggCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTa                                                                                                                                                 >  2:1442619/1‑207 (MQ=255)
                                                                                                                                                                                                                           gCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGc                                                     >  1:1776335/1‑289 (MQ=255)
                                                                                                                                                                                                                           gCCAATGGCAACGAT‑AGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCa                                                    >  1:304551/1‑289 (MQ=255)
                                                                                                                                                                                                                            ccAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCAtt                                                        <  1:431634/285‑1 (MQ=255)
                                                                                                                                                                                                                            ccAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAg                                                      <  1:214952/287‑1 (MQ=255)
                                                                                                                                                                                                                            ccAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGc                                                     <  1:1678428/288‑1 (MQ=255)
                                                                                                                                                                                                                            ccAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCa                                                    >  1:1877372/1‑289 (MQ=255)
                                                                                                                                                                                                                            ccAAAGGCAAAGATCAGAAAGATAGCGACTGTCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAg                                                      <  2:900199/287‑1 (MQ=255)
                                                                                                                                                                                                                                tGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTa                                                >  2:600971/1‑289 (MQ=255)
                                                                                                                                                                                                                                tGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTa                                                >  1:37687/1‑289 (MQ=255)
                                                                                                                                                                                                                                 ggCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTaa                                               <  1:1471217/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGc                                            >  2:1888163/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aCGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGc                                            >  2:1676927/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aCGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGc                                            >  1:706638/1‑288 (MQ=255)
                                                                                                                                                                                                                                         tAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCttt                                       <  1:19365/289‑1 (MQ=255)
                                                                                                                                                                                                                                         tAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCttt                                       <  2:1092250/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   aGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACAGACTCAGTGCTTACTCAGCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCAGTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTGAACTGTAACGAGAGTTTCTTCACATTAGTTTACATAATGTAAACACCCTTATCGTTTAGTGCCCtttacctccgga                              >  2:968015/1‑279 (MQ=255)
                                                                                                                                                                                                                                                    gCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGAt                             >  1:109786/1‑288 (MQ=255)
                                                                                                                                                                                                                                                     cGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGAt                             >  1:1891243/1‑287 (MQ=255)
                                                                                                                                                                                                                                                       aaTGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTtata                                                                                                                                                       >  1:248479/1‑163 (MQ=255)
                                                                                                                                                                                                                                                          gCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTgac                      <  2:1877372/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          gCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTgac                      <  1:1698806/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           cccAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTgac                      <  2:706638/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tgaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAgg             <  2:1875978/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tgaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAgg             <  2:1742123/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aGCGATGGCTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGAt           >  1:1564917/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     aGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGAt           >  2:1121393/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     aGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGAt           >  1:1461157/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             cTTGAGTGGGTGTTGACATACTCATTGCGTACTCATCAAAAGTAGCGCCAGGTTCACGGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCGTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGa    <  2:1699434/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ttCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGAtg  <  1:1528007/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                  
CCCAGCCGCTTTCGCGGATAGAGGTACTCCATGCGAACAGATACAGCGCTTCAACGTCGAAGATAACGAAGAACATGGCCACCAGATAAAA‑CTTGGCGGACAGGCGTAAGCGGGCGGAGCCGA‑CGCTGTCGATACCGGATTCAAACGGCACGTTTTTGCTCCTCGCGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTACTCATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATG  >  CP042583/1976069‑1976625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A