Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 566,020 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583566,0200AT99.2% 588.4 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (114/132);  minor base G (1/1);  total (115/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583566,0210CG99.6% 586.0 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (114/133);  minor base A (1/0);  total (115/133)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583566,0220TA99.6% 577.6 / NA 248intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (113/133);  total (115/133)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 248 total aligned reads displayed.

GCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACACTTTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGGCTTGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGCTCTTGCAG  >  CP042583/565745‑566295
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                 
gCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTAAGGGCAATCAATGATTCAGGTGttt                                                                                                                                                                                                                                                                        >  2:912539/1‑289 (MQ=255)
       cTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCAc                                                                                                                                                                                                                                                                 >  1:1616022/1‑289 (MQ=255)
       cTCAACAAGCGCCCATTGTCGGCTGGCTCCAGGGTCGTTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTAACTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCa                                                                                                                                                                                                                                                                  <  2:1695849/288‑1 (MQ=255)
         cAACAGACGCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCAcgc                                                                                                                                                                                                                                                               >  1:548897/1‑289 (MQ=255)
         cAACAGACGCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCAcg                                                                                                                                                                                                                                                                >  2:1746158/1‑288 (MQ=255)
           aCAGACTCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACgcgc                                                                                                                                                                                                                                                             <  1:1242620/289‑1 (MQ=255)
                cgggcgttgctggctgGCTCCAGGGTGCTTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGAc                                                                                                                                                                                                                                                         <  2:1433308/276‑1 (MQ=255)
                cgcgCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGAc                                                                                                                                                                                                                                                         >  1:1695849/1‑288 (MQ=255)
                cgcgCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGAc                                                                                                                                                                                                                                                         >  1:616646/1‑288 (MQ=255)
                cgcgCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGAc                                                                                                                                                                                                                                                         >  1:650563/1‑288 (MQ=255)
                    cATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAACGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCGGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACGGATTCCGGTGTTACTGCCACGCGCAGCCCCAtt                                                                                                                                                                                                                                                    >  2:19979/1‑289 (MQ=255)
                      ttGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTc                                                                                                                                                                                                                                                  <  1:887857/289‑1 (MQ=255)
                          ctgctgGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCt                                                                                                                                                                                                                                              <  1:1291204/289‑1 (MQ=255)
                           tgctgGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGCGTTTCTGCCTCGCCCAGACCCATTTCATCt                                                                                                                                                                                                                                              >  2:1264265/1‑288 (MQ=255)
                             ctgGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCAcgcg                                                                                                                                                                                                                                                              <  1:1609401/270‑1 (MQ=255)
                                    cAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGACGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGca                                                                                                                                                                                                                                    <  2:411182/289‑1 (MQ=255)
                                                 cgAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACcgc                                                                                                                                                                                                                        >  1:187818/1‑288 (MQ=255)
                                                 cgAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCAATGATTCCGGTGTTTCTGCACCGCGCAGACCCCTTCTATCTTCAGTACGCACCCCTTTACcgc                                                                                                                                                                                                                        >  2:1805174/1‑288 (MQ=255)
                                                       tCTTTGTTCAACCCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGACCATTCACCGGCCAATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCACTAAAGATGGGGATAAAGAGAGAAAAACCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCagag                                                                                                                                                                                                                  <  1:310310/288‑1 (MQ=255)
                                                               cAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGt                                                                                                                                                                                                         >  1:1505784/1‑289 (MQ=255)
                                                                 aaCCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCCGACCCATTTCCTCTTCAGTACGCACCACTTTACCGCGCAGCAAGTTCGGGTAg                                                                                                                                                                                                       >  2:359120/1‑289 (MQ=255)
                                                                 aaCCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAg                                                                                                                                                                                                       >  1:1418670/1‑289 (MQ=255)
                                                                 aaCCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCAACCTCAGTACCCCACAATTTACCGCCCAGAAATTTCGGGTAg                                                                                                                                                                                                       >  2:1425252/1‑289 (MQ=255)
                                                                     gTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCa                                                                                                                                                                                                                                           >  1:1590017/1‑249 (MQ=255)
                                                                      ttCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAACGTCGTTTTCTTTGCGGGTCCGGGCCATCCCTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCCCCACTTTACCGCCCCGCGAGTTCGGGTAGACCTc                                                                                                                                                                                                  >  2:454308/1‑289 (MQ=255)
                                                                       tCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCg                                                                                                                                                                                                 >  1:1409515/1‑289 (MQ=255)
                                                                       tCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCCGCCCCGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCg                                                                                                                                                                                                 >  2:965379/1‑289 (MQ=255)
                                                                       tCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGG‑AAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAACAAAAG‑‑CCACCGCAACACCAGGCCCGAGAAAAATGGGGGATAATAACACCCGCCCCGGGCTCGTTCTCTTCGCGTGCGGGCGATCTCTGATTCCCGGGTTTCTGCCACGCGCCGACCCCTTTTCTCTTTAGTCTCCACCCCTTTATCCCGCAGAGAGAGCGGGTGTAAGTCGGTa                                                                                                                                                                                              >  2:492343/1‑289 (MQ=255)
                                                                         ctctTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGt                                                                                                                                                                                               <  2:749402/289‑1 (MQ=255)
                                                                         ctctTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGt                                                                                                                                                                                               <  1:1797055/289‑1 (MQ=255)
                                                                         ctctTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGt                                                                                                                                                                                               <  2:1230704/289‑1 (MQ=255)
                                                                         ctctGATCTGGATGCCGCCAGAAGATGAACATTCACCGGCCTATAAGGAAATTAGTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGAGGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGt                                                                                                                                                                                               <  1:537946/289‑1 (MQ=255)
                                                                                   tATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGca                                                                                                                                                                                                                                    >  1:1516824/1‑242 (MQ=255)
                                                                                   tATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGca                                                                                                                                                                                                                                    <  2:1516824/242‑1 (MQ=255)
                                                                                     tGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCAACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGTCACGCGCAGACCCATTTCATCTTCAGTACGCCCCCCTTTACCGCGCAGAGAGGTCGGGTAGACGTCGGTAATTTCTACATc                                                                                                                                                                                   >  2:1618259/1‑289 (MQ=255)
                                                                                       ccgccgGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcga                                                                                                                                                                                 <  2:650563/289‑1 (MQ=255)
                                                                                        cgccgGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCa                                                                                                                                                                                                                                           <  1:690854/230‑1 (MQ=255)
                                                                                        cgccgGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcgac                                                                                                                                                                                >  1:1000279/1‑289 (MQ=255)
                                                                                        cgccgGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcgac                                                                                                                                                                                >  2:1348727/1‑289 (MQ=255)
                                                                                        cgccgGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATcgac                                                                                                                                                                                >  1:1380830/1‑289 (MQ=255)
                                                                                               aGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAAttt                                                                                                                                                                          <  1:1348727/288‑1 (MQ=255)
                                                                                                  tGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTAc                                                                                                                                                                        <  1:1824731/287‑1 (MQ=255)
                                                                                                  tGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTAc                                                                                                                                                                        <  1:1773329/287‑1 (MQ=255)
                                                                                                  tGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTAc                                                                                                                                                                        <  1:1939497/287‑1 (MQ=255)
                                                                                                           aCCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTTCGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATcc                                                                                                                                                             >  1:1123017/1‑289 (MQ=255)
                                                                                                           aCCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGTGAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATAccc                                                                                                                                                             >  1:47095/1‑289 (MQ=255)
                                                                                                              ggCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGc                                                                                                                                                          >  2:1785142/1‑289 (MQ=255)
                                                                                                                      aGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTc                                                                                                                                                  <  1:1898536/289‑1 (MQ=255)
                                                                                                                              attCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTg                                                                                                                                           >  2:889924/1‑288 (MQ=255)
                                                                                                                                tCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGAcc                                                                                                                                        <  1:403769/289‑1 (MQ=255)
                                                                                                                                   cGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTc                                                                                                                                                                                                                                                  <  2:362786/180‑1 (MQ=255)
                                                                                                                                    gCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcgc                                                                                                                                    >  1:600324/1‑289 (MQ=255)
                                                                                                                                                aGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTAACGATCATATCCGGCGTGCCTTCGAAGTTGACGACGCGGTTATTTTCCg                                                                                                                        >  1:840902/1‑289 (MQ=255)
                                                                                                                                                          aTTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGa                                                                                                              <  1:1746158/289‑1 (MQ=255)
                                                                                                                                                          aTTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGa                                                                                                              <  2:1384613/289‑1 (MQ=255)
                                                                                                                                                            tGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTCGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAAGCGGTTATTTTCCGTACGCCCGGaa                                                                                                             >  1:43200/1‑288 (MQ=255)
                                                                                                                                                            tGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATAACCGGCGTGCCTTCGACGTTGACCAAGCGTCTATTTTCCGTACCCCACGaaa                                                                                                            >  2:1580908/1‑289 (MQ=255)
                                                                                                                                                                    aGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCa                                                                                                      <  1:1269515/287‑1 (MQ=255)
                                                                                                                                                                       tGGGGATAAAGAGAGAACAAGCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCatga                                                                                                   <  1:1335606/287‑1 (MQ=255)
                                                                                                                                                                             tAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTa                                                                                           <  1:1728815/289‑1 (MQ=255)
                                                                                                                                                                               aaGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcg                                                                                                                                     <  2:807837/245‑1 (MQ=255)
                                                                                                                                                                                    agaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACgcgatg                                                                                    >  2:184841/1‑284 (MQ=255)
                                                                                                                                                                                    agaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACgcgatg                                                                                    >  1:250615/1‑284 (MQ=255)
                                                                                                                                                                                     gaAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACgcgatg                                                                                    <  1:359120/288‑6 (MQ=255)
                                                                                                                                                                                      aaaaaaCAAGGGCCACCGGAACGGGAGGCCTGAGAATGACGGCTGATGATAACCTACGCCAGGGTCGTTTTCTTTGCGGGTACGGGCAAGCCATGATTCCGGTGTTTCGGCCACGCGCAGACCCATTTCATCTGCAGTACGCACCACTTGACCGCGCAGAGAGTACGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGGTCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACgcgatgta                                                                                  <  2:1210891/289‑8 (MQ=255)
                                                                                                                                                                                          aaCAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCtt                                                                              <  1:1463250/289‑1 (MQ=255)
                                                                                                                                                                                              aGGCCCACCGGAACGGCAGGCCTGAGAATTTCGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTAc                                                                          <  2:1123017/289‑1 (MQ=255)
                                                                                                                                                                                                gCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCa                                                                        >  1:1548830/1‑289 (MQ=255)
                                                                                                                                                                                                  ccACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGa                                                                      <  1:912539/289‑1 (MQ=255)
                                                                                                                                                                                                  ccACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTCCTTTGCGGGTACGGGCAATCACTGATTCCGTTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGa                                                                      <  2:423253/289‑1 (MQ=255)
                                                                                                                                                                                                    aCCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAAt                                                                    >  1:777644/1‑289 (MQ=255)
                                                                                                                                                                                                           cGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACg                                                                                                                                                                                                                                      >  2:98856/1‑120 (MQ=255)
                                                                                                                                                                                                           cGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACg                                                                                                                                                                                                                                      <  1:98856/120‑1 (MQ=255)
                                                                                                                                                                                                            tgccggacTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCACACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTggg                                                            <  2:1270467/282‑1 (MQ=255)
                                                                                                                                                                                                            ggCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTggg                                                            <  1:180609/289‑1 (MQ=255)
                                                                                                                                                                                                                   cTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAACGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATAAGCTGGgtggtggc                                                     >  1:138975/1‑287 (MQ=255)
                                                                                                                                                                                                                       gaATTACGGCGGATAAGACCCCACGCCAAGGTCGTTTTCGTGGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAg                                                  <  2:1000279/288‑1 (MQ=255)
                                                                                                                                                                                                                           tACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGAt                                                                                                                                                                    >  2:286326/1‑170 (MQ=255)
                                                                                                                                                                                                                             cGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCcg                                            >  1:1027340/1‑288 (MQ=255)
                                                                                                                                                                                                                                       taaCCCACGCTAAGGTCATGTTCTTTGCGGGTACAGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCCCTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACg                                 <  2:1190383/289‑1 (MQ=255)
                                                                                                                                                                                                                                       taaCCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACg                                 <  1:1210348/289‑1 (MQ=255)
                                                                                                                                                                                                                                          cccACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCa                              >  1:1067549/1‑289 (MQ=255)
                                                                                                                                                                                                                                           caaCGCCAAGGTCGTGTTCTTTGCGGGTAAGGGCAATCACGGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTCCCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATAGACGAATGTACCGATCATATCCGGCGTGCCTTCGAAGTTGCCCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCAt                             <  2:1937878/287‑1 (MQ=255)
                                                                                                                                                                                                                                             aCGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCatg                                                                                                    <  2:1727995/216‑1 (MQ=255)
                                                                                                                                                                                                                                             aCGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGAAGCTCTTACTCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGGTCCACCCCCTc                            >  2:1867264/1‑288 (MQ=255)
                                                                                                                                                                                                                                              cGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCAcg                                                                                                                                     <  2:1060179/182‑1 (MQ=255)
                                                                                                                                                                                                                                              cGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCCTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCCGAATACGCTGGGTGGTGCTGAGCATCCGGAGCATCCCTGCCATCGc                          >  2:1596286/1‑289 (MQ=255)
                                                                                                                                                                                                                                               gCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGTCTCCACGCCATCGCt                         >  1:827961/1‑289 (MQ=255)
                                                                                                                                                                                                                                               gCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCt                         >  1:954333/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 cAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTg                       <  1:184841/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   aGGTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCg                                                                                                                        <  1:1039319/190‑1 (MQ=255)
                                                                                                                                                                                                                                                    ggTCGTTTTCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCttccgg                    >  2:603217/1‑285 (MQ=255)
                                                                                                                                                                                                                                                         ttttCTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTaa               >  2:1716062/1‑289 (MQ=255)
                                                                                                                                                                                                                                                             cTTTGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATgcg           <  1:289494/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                tGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATgcgc          >  2:1480990/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                tGCGGGTACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGctc        <  1:492343/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGGATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGCTCTTGCa   <  2:250615/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGCTCTTGCa   <  1:454308/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGCTCTTGCa   <  1:1893225/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tACGGGCAATCACTGATTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTGCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGCGATGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGCTCTTGCa   <  1:228794/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                 
GCACAGGCTCAACAGACGCGCATTGTCTGCTGGCTCCAGGGTGATTTCGCGAGTGTCTATGTTCAAACCGTTCCTCTTATCTGTATGCCGCCGGAAGCTGAACATTCACCGGCCTATAAGGAAATTATTCACGCCACAGGAAAAAGGCGCAAGCATTGCAATAAAGATGGGGATAAAGAGAGAAAAAACAAGGCCCACCGGAACGGCAGGCCTGAGAATTACGGCTGATAATAACCCACGCCAAGGTCGTTTTCTTTGCGGGTACGGGCAATCACACTTTCCGGTGTTTCTGCCACGCGCAGACCCATTTCATCTTCAGTACGCACCACTTTACCGCGCAGAGAGTTCGGGTAGACGTCGGTAATTTCTACATCGACGAATTTACCGATCATATCCGGCGTGCCTTCGAAGTTGACCACGCGGTTATTTTCCGTACGCCCGGAAAGCTCCATGATGCTCTTACGGCTTGTACCTTCTACCAGAATACGCTGGGTGGTGCCGAGCATCCGGCGGCTCCACGCCATCGCTTGCTGATTAATGCGCTCTTGCAG  >  CP042583/565745‑566295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A