Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 745,983 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583745,9830AT98.1% 622.1 / NA 270intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base T (148/115);  minor base G (1/4);  total (150/120)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583745,9840CG98.9% 623.1 / NA 270intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base G (149/116);  minor base T (1/2);  total (150/120)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583745,9850TA98.9% 633.9 / NA 270intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (148/118);  minor base G (1/2);  total (150/120)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

TTGCGGATCGGTAATGCAAAACAGACGCGATACCAGCTTTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGGCTCCAGGCAATGACGACGGCCAGCACCATTAAAAGACTGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAAACTGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAAGCTGATCATCCACGGAGCGCTGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAG  >  CP042583/745705‑746264
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                       
ttGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATccg                                                                                                                                                                                                                                                                                 <  1:1028606/289‑1 (MQ=255)
      aTCGGTAATGCAAAACAGACGCGATACCACGATGTCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGATCATATCCAGCGTCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCa                                                                                                                                                                                                                                                                            <  1:96969/288‑1 (MQ=255)
       tCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATCAAAAGTGAGCCGTACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGAGAAAATAACCGTACCGTTAACCCTTGGGAAGTAGGCATCCGGCGCatg                                                                                                                                                                                                                                                                          >  2:712159/1‑289 (MQ=255)
           tAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCatgatg                                                                                                                                                                                                                                                                       <  1:1319129/288‑1 (MQ=255)
                cAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGACAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGaa                                                                                                                                                                                                                                                                 >  1:43980/1‑289 (MQ=255)
                    aCAGGCTCGATACCACGATTGGCGACGTTGATAGAGTTTATGCCCCATCGCTTGACAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTta                                                                                                                                                                                                                                                             <  2:786014/289‑1 (MQ=255)
                     cAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTtat                                                                                                                                                                                                                                                            >  2:1627600/1‑289 (MQ=255)
                     cAGACGCGATACCACGATCGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGGGAAAATCACCGTACCGTGAACGCTTGGGAATGAGTCATCCGCCGCACGATGCAGGAAGTtat                                                                                                                                                                                                                                                            >  1:1761440/1‑289 (MQ=255)
                        aCGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAg                                                                                                                                                                                                                                                          >  1:1413979/1‑288 (MQ=255)
                        aCGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATACAGCGGCCAGTGGACGCCAAGATAAACGCGCGGCCAGGCAATGAAGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGGCAGCCGAAAAATGCCAGTGCAAAGATGAAAATAACAGTACGGTGCTCGACTGGGGATGGGTCATCCGCCGCATTAAGCAGGCAGtgtgtg                                                                                                                                                                                                                                                          >  2:547300/1‑283 (MQ=255)
                          gcgATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTACCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAACAGTGCGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAAGCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAGGTTTGAGCCg                                                                                                                                                                                                                                                       >  2:1912858/1‑289 (MQ=255)
                            gATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGat                                                                                                                                                                                                                                                     <  1:1462215/289‑1 (MQ=255)
                                 cacgattGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATAtttt                                                                                                                                                                                                                                                <  2:1139658/284‑1 (MQ=255)
                                           gACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAgggg                                                                                                                                                                                                                                      >  2:345428/1‑289 (MQ=255)
                                                tGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAgg                                                                                                                                                                                                                                                                   <  1:147320/255‑1 (MQ=255)
                                                 gATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGggtcggtc                                                                                                                                                                                                                                >  1:1716182/1‑289 (MQ=255)
                                                      agTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCg                                                                                                                                                                                                                           >  2:481225/1‑289 (MQ=255)
                                                        tttATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGCGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGa                                                                                                                                                                                                                         >  1:1169538/1‑289 (MQ=255)
                                                          tATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGaaa                                                                                                                                                                                                                       >  2:1175857/1‑289 (MQ=255)
                                                             gCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAg                                                                                                                                                                                                                    <  1:413472/289‑1 (MQ=255)
                                                                ccctagCTTGCCAGGAAATCTGGGCACTAGGGCAGCCAATCATACCTGCCAGCAATCCCCCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATg                                                                                                                                                                                                                 <  2:1477164/284‑1 (MQ=255)
                                                                  aTCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGt                                                                                                                                                                                                                >  1:1553705/1‑288 (MQ=255)
                                                                           ccgatgaatggcgCACAAAGGCAGGCAATCAAACGTGCCAGCAATCCACCGAGCATATCAAGAGGCCAGTGAACGTAAAGATAAGCGAGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGGGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGGGCAAAGGTGAAAATCTCCTTAACGTTATCGCTTGGGAATGAGTCAGCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTcc                                                                                                                                                                                                      <  2:1649679/277‑1 (MQ=255)
                                                                           cAGCTAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTcc                                                                                                                                                                                                      <  2:375137/289‑1 (MQ=255)
                                                                              aTAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCAT‑AAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAgg                                                                                                                                                                                                   >  1:52506/1‑288 (MQ=255)
                                                                                     gggCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACaa                                                                                                                                                                                             >  1:1857330/1‑288 (MQ=255)
                                                                                       gCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATACAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATAACCGTACCATGATCGCTTTGAAATGAGGCATCCGCCGCATGATGCAGGAAGTTATAGACGATATTTTCGACAAAGGGTCTGTCGTGAGGAAAAAGATGTCCAATCGTCAAGGACACAAGCa                                                                                                                                                                                          >  1:690991/1‑289 (MQ=255)
                                                                                         aCTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAgg                                                                                                                                                                                        <  1:134613/289‑1 (MQ=255)
                                                                                                  gCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGGTGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTAACGTGATCGCTTGGGAATGAGTAATCCGCCGCAAGATGCAGGAAGTTATAGCCGATACTTTAGACAAAGGGACGGCCGTGCGGAAAAAGTAGTCCCAGCGCTACGGACACAAACAGCCGGACCGct                                                                                                                                                                               >  2:216260/1‑288 (MQ=255)
                                                                                                   ccAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCat                                                                                                                                                                                                                                                                           >  2:166466/1‑196 (MQ=255)
                                                                                                   ccAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCa                                                                                                                                                                              >  1:1034949/1‑289 (MQ=255)
                                                                                                            ccTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATa                                                                                                                                                                      >  1:1676162/1‑288 (MQ=255)
                                                                                                               gCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATccgccg                                                                                                                                                                                                                                                                              >  1:957095/1‑181 (MQ=255)
                                                                                                               gCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGACAGCGa                                                                                                                                                                  >  1:907814/1‑289 (MQ=255)
                                                                                                                 cAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGAtt                                                                                                                                                                >  1:272740/1‑289 (MQ=255)
                                                                                                                    cAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACACGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGCAAAAAGATTGCCCATAGTCAAGGACACAAACAGGCTGACCGCAAGCACGATAGAGCttttt                                                                                                                                                             >  2:1786087/1‑289 (MQ=255)
                                                                                                                     aaTCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGAttt                                                                                                                                                               <  1:481225/286‑1 (MQ=255)
                                                                                                                              aGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGtt                                                                                                                                                   >  1:885549/1‑289 (MQ=255)
                                                                                                                                 atatCCAGCGGCCAGTGGACGCCAAGATAAACCCGCTACCAGTCAATGACGACGGCCAGCACCATAAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGCTCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTg                                                                                                                                                  <  1:216260/287‑1 (MQ=255)
                                                                                                                                  tatCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCg                                                                                                                                               <  1:1898815/289‑1 (MQ=255)
                                                                                                                                    tCCAGCGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGtt                                                                                                                                             <  2:272740/289‑1 (MQ=255)
                                                                                                                                     cccgCGGCCAGTGGGCGCAAAGAAAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTtg                                                                                                                                            <  2:1889659/286‑1 (MQ=255)
                                                                                                                                         cGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATAttt                                                                                                                                                                                                                                                 >  1:496997/1‑184 (MQ=255)
                                                                                                                                         cGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATAttt                                                                                                                                                                                                                                                 <  2:496997/184‑1 (MQ=255)
                                                                                                                                         cGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTtgt                                                                                                                                           >  1:1917470/1‑286 (MQ=255)
                                                                                                                                         cGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTtgt                                                                                                                                           >  1:1776824/1‑286 (MQ=255)
                                                                                                                                         cGGCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGACATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTtgt                                                                                                                                           >  1:1131627/1‑286 (MQ=255)
                                                                                                                                          ggCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGa                                                                                                                                                                                                  >  2:259521/1‑230 (MQ=255)
                                                                                                                                          ggCCAGTGGACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGa                                                                                                                                                                                                  <  1:259521/230‑1 (MQ=255)
                                                                                                                                                 ggACGCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAg                                                                                                                                   <  1:616883/286‑1 (MQ=255)
                                                                                                                                                   gagcaaaGATAAACGCGACACCAGGCAAAGAAGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGcccc                                                                                                                               <  2:139445/283‑1 (MQ=255)
                                                                                                                                                     gCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCcac                                                                                                                            <  2:189837/289‑1 (MQ=255)
                                                                                                                                                     gCCAAGATAAACGCGCGACCAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCcac                                                                                                                            <  1:1743895/289‑1 (MQ=255)
                                                                                                                                                                    cgaccAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCATTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACg                                                                                                             >  2:1511076/4‑289 (MQ=255)
                                                                                                                                                                    cgaccAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACg                                                                                                             >  2:142894/4‑289 (MQ=255)
                                                                                                                                                                       ccAGGCAATGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGcc                                                                                                          <  2:966494/289‑1 (MQ=255)
                                                                                                                                                                       ccAGGCAATGACGACGGCCAGCACCATTAAAAGGGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGcc                                                                                                          <  1:372166/289‑1 (MQ=255)
                                                                                                                                                                            cAAAGACGACGGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCa                                                                                                      <  1:682591/288‑1 (MQ=255)
                                                                                                                                                                                 acgacgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCCCAACCAAAGTACCCCGGCCAGCAACGGc                                                                                                 >  2:1275671/1‑288 (MQ=255)
                                                                                                                                                                                 acgacgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGACAGCGCGATCGCGACTTTTATCCCGAGGTGCCGTTGTTCGATAACGCCCCCCCACCAAAGTACTACGGCCaagacactc                                                                                                 >  2:1854939/1‑280 (MQ=255)
                                                                                                                                                                                  cgacgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGcac                                                                                               <  1:1888300/289‑1 (MQ=255)
                                                                                                                                                                                     cgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGa                                                                                                                                                                                                                         <  2:1449159/164‑1 (MQ=255)
                                                                                                                                                                                     cgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGa                                                                                                                                                                                                                         >  1:1449159/1‑164 (MQ=255)
                                                                                                                                                                                     cgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGcaccac                                                                                            >  1:1090031/1‑289 (MQ=255)
                                                                                                                                                                                     cgGCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGcaccac                                                                                            >  1:161356/1‑289 (MQ=255)
                                                                                                                                                                                      ggCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACg                                                                                           <  1:1107645/289‑1 (MQ=255)
                                                                                                                                                                                      ggCCAGCACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACg                                                                                           <  1:1175857/289‑1 (MQ=255)
                                                                                                                                                                                            cACCATTAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGt                                                                                                                                              >  2:436240/1‑232 (MQ=255)
                                                                                                                                                                                                 ttAAAAGTGAGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAATCCCCCACAACCAAAGTACCACGGCCAGCAACGGCAACACGGTAATCaaa                                                                                  >  1:742960/1‑287 (MQ=255)
                                                                                                                                                                                                  taaaagtgagCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAATAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTAACACGGCCAGCAACGGCACCACGGTAATCAAATCt                                                                               >  1:1813086/10‑289 (MQ=255)
                                                                                                                                                                                                  taaaagtgagCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTTCCGTTGTGCTGTAAGCCCCCACAACCAAAGTCACACGGCCAGCAACGCCACCACGGTAATCAAATCt                                                                               >  2:1764476/10‑289 (MQ=255)
                                                                                                                                                                                                   aaaagtgagCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCtt                                                                              <  2:885549/281‑1 (MQ=255)
                                                                                                                                                                                                    aaagggagCCGAACCCCAGGCGATGCCAGCATAAAAATGCCAGGGCAAAGGTGAAAATCGCCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCAGGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCttt                                                                             <  2:1572000/282‑1 (MQ=255)
                                                                                                                                                                                                         gagCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGACGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCaa                                                                        >  2:691555/3‑289 (MQ=255)
                                                                                                                                                                                                         gagCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCa                                                                         <  1:796216/286‑1 (MQ=255)
                                                                                                                                                                                                         gagCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTAATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCaa                                                                        >  1:1834665/3‑289 (MQ=255)
                                                                                                                                                                                                          agCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAAt                                                                       >  2:395187/2‑289 (MQ=255)
                                                                                                                                                                                                          agCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAAACAAATCCTTAGCAAt                                                                       >  2:658826/2‑289 (MQ=255)
                                                                                                                                                                                                                     aGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCa                                                            >  1:169364/1‑289 (MQ=255)
                                                                                                                                                                                                                         gATGCCAGCCGAAAACGGCACGTGCACACGTGAAAATCACCTTACCGTTTTCGCTTGGGCAGTGGTCAACCGCCGCATGATGCAGGAAGTGATAGCCGATATTTTCGACAAAGGGTCGGTCGGTCTGAAAAAGATGTTCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCCGTTTCCGGTGTGCTGGAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCaacg                                                         <  2:901564/288‑3 (MQ=255)
                                                                                                                                                                                                                              cAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCaacgagatc                                                    <  2:592079/288‑8 (MQ=255)
                                                                                                                                                                                                                                 cATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCg                                                                                                                                               >  2:299006/1‑194 (MQ=255)
                                                                                                                                                                                                                                  aTAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCa                                               <  2:832579/289‑1 (MQ=255)
                                                                                                                                                                                                                                  aTAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTAGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGatcat                                                  <  1:1575689/286‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaaaTGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACg                                             <  2:1716182/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaaTGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACgg                                            >  2:1119000/1‑289 (MQ=255)
                                                                                                                                                                                                                                      aaaTGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGa                                           >  2:414339/1‑289 (MQ=255)
                                                                                                                                                                                                                                       aaTGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAg                                          <  1:1627600/289‑1 (MQ=255)
                                                                                                                                                                                                                                           ccAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAg                                                                                                                                   >  1:1102864/1‑196 (MQ=255)
                                                                                                                                                                                                                                                       tGAAAATCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTc                            <  2:488642/287‑1 (MQ=255)
                                                                                                                                                                                                                                                       tGAAAATCACCGTACCGTGAGCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTc                            <  1:801213/287‑1 (MQ=255)
                                                                                                                                                                                                                                                         aaaaTCACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGatcat                                                  >  2:1746842/1‑263 (MQ=255)
                                                                                                                                                                                                                                                              cACCGTACCGTGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTtaata                   <  1:193560/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                ccgtaccgtGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAg                                                                                                                                   >  2:937501/1‑175 (MQ=255)
                                                                                                                                                                                                                                                                 cgtaccgtGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGACACGAGTCTGGCGTCGCGTTAATAaga                >  2:225808/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  gtaccgtGATCGGTTGGGAATGAGGCAGCCGCCGCATGATGCAGGAAGGTATTGCCGATATTTTCGACAAAGGGTGGGTCGTGCGGAAAAAGAGGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCGGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAagag               <  2:1324794/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCcac                                                                                                                            >  1:298773/1‑174 (MQ=255)
                                                                                                                                                                                                                                                                        tGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATa         >  2:1082530/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        tGATCGCTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATa         >  1:119503/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              cTTGGGAATGAGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATagagaga   >  2:1220763/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                       
TTGCGGATCGGTAATGCAAAACAGACGCGATACCAGCTTTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGGCTCCAGGCAATGACGACGGCCAGCACCATTAAAAGACTGCCGGACCACAGGCGATGCCAGCATAAAAATGCCAGTGCAAAGGTGAAAATCACCGTACCGTGATCGCTTGGGAAACTGTCATCCGCCGCATGATGCAGGAAGTTATAGCCGATATTTTCGACAAAGGGTCGGTCGTGCGGAAAAAGATGTCCCATCGTCCAGGACACAAACAGGCTGACCGCCAGCGCGATAGCGATTTTTATCACCAGTTGCCGTTGTGCTGTAAGCCCCCACAACCAAAGTACCACGGCCAGCAACGGCACCACGGTAATCAAATCTTTAGCAATAAAAATCGCCAAGCTGATCATCCACGGAGCGCTGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAG  >  CP042583/745705‑746264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A