Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 768,455 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583768,4550AT99.0% 478.5 / NA 205intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (92/111);  minor base G (2/0);  total (94/111)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP042583768,4560GC99.0% 483.1 / NA 205intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (92/111);  minor base A (2/0);  total (94/111)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583768,4570TA99.0% 473.8 / NA 205intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (92/111);  minor base C (2/0);  total (94/111)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

TGGCTGGCAAAAAAGCGCCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGAGTTTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAC  >  CP042583/768175‑768734
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
  gctggcACAAAGCTGCCGGAGTGAACTATGTGGCCCCGTTTGAACCTGCATGGATTCAGGAGCTTATTCAACTGGCGAAAGTTCTTATTGCCCGTCATAACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCCCCGCAGCGCAAAGACGATCCGGGGCCATTATTGCCGTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTgg                                                                                                                                                                                                                                                                                 <  2:49293/288‑1 (MQ=255)
  gctggcAAAAATCGGCCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGa                                                                                                                                                                                                                                                                                >  1:1710444/1‑289 (MQ=255)
            atcggcCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcg                                                                                                                                                                                                                                                                      <  1:1910021/285‑1 (MQ=255)
            atcggcCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcg                                                                                                                                                                                                                                                                      <  1:243213/285‑1 (MQ=255)
                gcCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTACTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcgcgc                                                                                                                                                                                                                                                                   <  2:263797/288‑1 (MQ=255)
                gcCGGAGGGAAAAATTTGGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCGGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcgcgc                                                                                                                                                                                                                                                                   <  2:1059506/288‑1 (MQ=255)
                 caggtggaaaaTGTTTAGCCCAGTTTGATCCGGACCATGTTCAGGCTATTATCACCATTACTAATGATATTGTTGCCATTTATCCCAGCAAGCCGAAAAACGTAGTGGCAAATGCGGCTATCGTACCGCAGCGCAAAGACGAGCCGGGTCCGTTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATGGGTGCCTGGCCGTATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCt                                                                                                                                                                                                                                                                  <  2:1194473/280‑1 (MQ=255)
                  cGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTa                                                                                                                                                                                                                                                                 <  1:875548/288‑1 (MQ=255)
                  cGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTa                                                                                                                                                                                                                                                                 <  1:494043/288‑1 (MQ=255)
                        gAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTAt                                                                                                                                                                                                                                                          <  1:208755/289‑1 (MQ=255)
                        gAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTAt                                                                                                                                                                                                                                                          <  1:1635852/289‑1 (MQ=255)
                        gAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTAt                                                                                                                                                                                                                                                          <  1:1435203/289‑1 (MQ=255)
                        gAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTAt                                                                                                                                                                                                                                                          <  1:1390815/289‑1 (MQ=255)
                           atatTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCTGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTCGGGTTATGAc                                                                                                                                                                                                                                                       >  2:1031591/1‑289 (MQ=255)
                              tttgGCCGCGTTTGACACGGCACAGATTCAGGCGCTTCTGACCCTGGCGAGAGACATTAGTGCCCGTTATCACACCAAGCCGGAAAAGGTAGTGGCACCTGGAGGTACCGCACCGCAGCGCAAAGCCGATCAGGGGCTATTATTTCCCTGGCAGCAACCGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGGTAACTTTTCCCTTGCCGGGCGCGCGCCGCACATACCTGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGtt                                                                                                                                                                                                                                                    <  2:426436/289‑1 (MQ=255)
                               tttGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTTGCAGCAACTGGCGCAGCAGGGGCTTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTCGATACCGCGTCACTGCTGGAGCTTTTGGCGCGCTCCGGTTCGTACGTTa                                                                                                                                                                                                                                                   >  2:960108/1‑289 (MQ=255)
                                   ccccGTTTAAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTTGCACATGCGGATATCGCCCCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACGGGCGCAGCAGGGGATTGGTGCCTGGCCGTATGCGCACCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                                                                                                                                                                                                               <  1:512071/289‑1 (MQ=255)
                                    cccGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                                                                                                                                                                                                               <  2:1719890/288‑1 (MQ=255)
                                    cccGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                                                                                                                                                                                                               <  1:1733413/288‑1 (MQ=255)
                                    cccGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                                                                                                                                                                                                               <  1:408538/288‑1 (MQ=255)
                                             aCCAGCACAGAGTCAGACGCTTATTCCACTGGCGAAAGATTTTATTGCCCGGTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCCGACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCGGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGac                                                                                                                                                                                                                                      <  2:186559/288‑1 (MQ=255)
                                                  cacaGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACcgc                                                                                                                                                                                                                                 <  1:183158/288‑1 (MQ=255)
                                                  cacaGATTAAGGCGCTAAGTCCACTGGCGAAACAAATGATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGAAAAGACGATCCGGGGCAATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGAATCGCAGCGGGTTAACTTTTCCCTTGCCTGGGGCGCGCCGAACACACCGGTAGATCCCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACcgc                                                                                                                                                                                                                                 <  2:695612/288‑1 (MQ=255)
                                                         ttaGGTGCTTCATCCGCTGGCTAAAGAACTGATTGCCCGTTCTCACCTGAAGACAGGAAACGGGTTGGCACATGCGTTGATGGTACCGCAGCGCAAGGACGATCCGGGGCCATCAGTGCCCTGGTAGCAACTGGCGCAGCAGGGGATTGGGGCCTGGCCGGATGCGCAGCTGGTTAACTTGTCCGTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTACACCTGATATGGCACCGCGCGagcag                                                                                                                                                                                                                         <  2:317169/287‑1 (MQ=255)
                                                               gcTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGgcgc                                                                                                                                                                                                                   <  1:696584/289‑1 (MQ=255)
                                                                 ttATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcgcgc                                                                                                                                                                                                                                                                   <  1:1168000/239‑1 (MQ=255)
                                                                 ttATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGt                                                                                                                                                                                                                 >  1:1782400/1‑289 (MQ=255)
                                                                 ttATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGACGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGt                                                                                                                                                                                                                 >  1:1480972/1‑289 (MQ=255)
                                                                       cACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTAt                                                                                                                                                                                                           <  1:1281271/289‑1 (MQ=255)
                                                                         cTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAgcggc                                                                                                                                                                                                                     <  1:968938/277‑1 (MQ=255)
                                                                           ggCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGAAGTTAAACCTGATATGACACCGTGCGAGCACGGGTGCGCGATTATGGCa                                                                                                                                                                                                       >  1:309507/1‑289 (MQ=255)
                                                                           ggCAAAAGATATTATTGCCCGTTATAGCATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGc                                                                                                                                                                                                        <  2:1094547/288‑1 (MQ=255)
                                                                             cGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCAt                                                                                                                                                                                                      >  1:912413/1‑288 (MQ=255)
                                                                               aaaGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTg                                                                                                                                                                                                                                                                         >  2:363229/1‑219 (MQ=255)
                                                                               aaaGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTg                                                                                                                                                                                                                                                                         <  1:363229/219‑1 (MQ=255)
                                                                                aaGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑cc                                                                                                                                                                                                  <  1:1453089/288‑1 (MQ=255)
                                                                                   atatTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTGAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTAATTGCTGGGGCGTTTGGCGCGGTACGGTTAGGACGGTAAACCTTGTATGACCACGCGCGAGCAGCGGGGCGTGAATagtctggcgcttaa                                                                                                                                                                                               >  2:968938/1‑276 (MQ=255)
                                                                                            cccGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAcc                                                                                                                                                                                                                                               <  2:662984/232‑1 (MQ=255)
                                                                                            cccGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTcc                                                                                                                                                                                      >  1:1147037/1‑288 (MQ=255)
                                                                                              cGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGTTCCGGGGCCATTATTTCGCTGGCAACAACTGGCGCAGCAGGGGTGTGGTGCCTGGCCGGATGCCAAGCGGGTTAACTTTCACCTTGCCTGGCGCGCGCGGGCCACACCGCTAGATACCTCGGCAGTGCTGGACCTCTTGGCGCGCTCCGGTTATGACGTTAAACCTGCTATGACACTACCCGGGCAGGCGCGCGTTATTTTGGGGTT‑CCatgtgtagttgcgt                                                                                                                                                                                    >  2:778014/1‑275 (MQ=255)
                                                                                              cGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCgt                                                                                                                                                                                    >  1:1258868/1‑287 (MQ=255)
                                                                                              cGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGcc                                                                                                                                                                                   <  1:1031591/289‑1 (MQ=255)
                                                                                              cGTTATAACATCAAGCCGGAAAACGTAGTGGCACATGCCGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGcc                                                                                                                                                                                   <  1:97774/289‑1 (MQ=255)
                                                                                                ttATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACTCCGGTAGATACCCCCTCCTTGCTGGAGCTTTTTGCGCGCTACGGGTTAGACGTTAAACCTTATATTACACCCCCCCACCCGCGGCCCGTGTCTATGGCATC‑TCAGTGTCATTTCCGccca                                                                                                                                                                                 >  2:1467209/1‑288 (MQ=255)
                                                                                                ttATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCg                                                                                                                                                                                 >  2:325921/1‑289 (MQ=255)
                                                                                                 tATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGa                                                                                                                                                                                <  1:661207/289‑1 (MQ=255)
                                                                                                 tATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGa                                                                                                                                                                                <  1:994135/289‑1 (MQ=255)
                                                                                                          aggCCGGAAAACGTAGTGGCACATGCGGGTACCGGACCGCAGGGCAAAGCCGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCGGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTtatata                                                                                                                                                                       <  2:57320/287‑1 (MQ=255)
                                                                                                          aaGCCGGAAAACGTAGTGGCACATCCGGATATCGCACCGCGGCGCAAAGACGACCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGTTGCGCACCGGGGTAACTTTTACCGTGCCGGGCGCGCGCCGCACACACCGGTATATACCGCTTCATTGCCTGAGCGTTTGTAGCGCTACTGTTATGACGGTAAACCTGATATTCCACTGCTCGAGCAGCCACGCGGGATGTCGGGATTCCCATAT‑CATTTCCGACCGTGGGtatata                                                                                                                                                                       >  2:680540/1‑289 (MQ=255)
                                                                                                           aGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACg                                                                                                                                                                              >  2:1278444/1‑281 (MQ=255)
                                                                                                           aGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTtatata                                                                                                                                                                       >  1:672554/1‑288 (MQ=255)
                                                                                                             ccGGAAAACGTAGTGGCACATCCGGATATCGCACCGCCGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGCAGATACCGCCTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGTCACCCCCCGCGCAGAGGAGCGTGACTCTGCCATT‑CCATATGACTTTCTGCCCGCGGTTAACTAACg                                                                                                                                                                    >  2:189005/1‑289 (MQ=255)
                                                                                                                                 tGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACCGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATAATGGCATT‑CCAGATGCATTTCCGCCCGACGCTAACTAACGGCGAAGGCGATGCAGAAAc                                                                                                                                                 >  2:1166376/1‑288 (MQ=255)
                                                                                                                                  gCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTc                                                                                                                                               >  1:1225025/1‑289 (MQ=255)
                                                                                                                                          ggCACGGCAGCGGAAAGACGTTCCGGGGCGATTAGTTCCCTGGCAGGGAGTGGCGCAGGAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACGTTGGCGGGCGGGCGCCGCACACACCGGTAGATACCGCGTCAGTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGAt                                                                                                                                        <  2:1563770/287‑1 (MQ=255)
                                                                                                                                          cGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGAtt                                                                                                                                       >  1:1052372/1‑289 (MQ=255)
                                                                                                                                            cACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGa                                                                                                                                                          <  2:1484661/268‑1 (MQ=255)
                                                                                                                                            cACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGa                                                                                                                                                          >  1:1484661/1‑268 (MQ=255)
                                                                                                                                                gCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATAGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGcc                                                                                                                                                                                   >  1:1477806/1‑239 (MQ=255)
                                                                                                                                                 cAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGcgcg                                                                                                                                                                                                                  >  1:942772/1‑208 (MQ=255)
                                                                                                                                                  aGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAg                                                                                                                                <  1:325921/288‑1 (MQ=255)
                                                                                                                                                         aGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCCCGTCATTGCTGGAGCTTTTGGCGCGCTACGGGTATGACGGTAAACCTGATATTACACCGCGCGAGCAGCCGCGCCTGATTATGGCATT‑ACATATTCATTTCCTCCCGCCGTTATATCACTGCCAAGCGGATGCAGCAACTACGGGCATTTCCCAAGCATTGccg                                                                                                                        >  2:1634417/1‑287 (MQ=255)
                                                                                                                                                            cGATACGGGGCCATTATTGCCCTGGCAGCAACTGGCGGAGCAGGGGATTGGGGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGGGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGga                                                                                                                      <  1:64907/288‑1 (MQ=255)
                                                                                                                                                                 cGGGGCAATTATTTCCCGGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCGGGCCGGATGCGCAGCGGGTTAACTTTAACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATa                                                                                                                <  2:305041/289‑1 (MQ=255)
                                                                                                                                                                      ccATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACggg                                                                                                            <  2:520235/288‑1 (MQ=255)
                                                                                                                                                                         ttattTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATAAGGGGAgg                                                                                                        >  1:46171/1‑289 (MQ=255)
                                                                                                                                                                         ttattTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATGATGGCATT‑CCAGATGCATTTCCGCCCGACGTTAGATAACGGCGAGCAGGATGCAGAAACTCAGGCAATTTCCGAAACATTTCTGGAGAAATAAGGGCAgg                                                                                                        >  2:1442206/1‑289 (MQ=255)
                                                                                                                                                                                ccTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGcg                                                                                                 >  1:1916192/1‑289 (MQ=255)
                                                                                                                                                                                ccTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGAAGGATTagc                                                                                                  >  1:1878486/1‑286 (MQ=255)
                                                                                                                                                                                    gcagcaACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGGAATAAGGGCAGGATTAGCGCGGc                                                                                             >  1:1788363/1‑289 (MQ=255)
                                                                                                                                                                                              gcgcAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGa                                                                                                                                                          >  1:1665804/1‑218 (MQ=255)
                                                                                                                                                                                                cacgcagTGGCTTGGTGCCTGGCCGTGTGCGCCGCGGGTTAACTTTTACCTGGCCGGGCGCGCGCCGCACAACCCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCGACGGTTATGACGTTAAACCTGATATGACACCGCGCGGGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                                                  <  2:1147037/285‑1 (MQ=255)
                                                                                                                                                                                                        ggATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCACT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGGCGCGTAg                                                                         >  1:1344410/1‑289 (MQ=255)
                                                                                                                                                                                                              gTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTTCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggc                                                                   >  1:256615/1‑289 (MQ=255)
                                                                                                                                                                                                                   tGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcggccg                                                              >  1:1647887/1‑287 (MQ=255)
                                                                                                                                                                                                                     gacGGCTGAGCAGCGGGTCCACTTTGCCGTTGCCGGGCGCGCGCGGCACACACCGGTAGTTACCGCGTCATTGCGGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCGGATATGACACCGCGCGAGCAGGGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGt                                                             <  2:950914/286‑1 (MQ=255)
                                                                                                                                                                                                                     gCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑cc                                                                                                                                                                                                  >  1:716849/1‑155 (MQ=255)
                                                                                                                                                                                                                     gCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑cc                                                                                                                                                                                                  <  2:716849/155‑1 (MQ=255)
                                                                                                                                                                                                                      ccggggacgctgtgggTTGACTATTACCTTGCCGGGCGGCGGCCACACACACCGGGAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTAGGACGTTAAACCTGATATGGCACCGCGCGAGCAGCGGCGCGGGATTAGGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGTCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATGCGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtg                                                            <  2:1258868/275‑2 (MQ=255)
                                                                                                                                                                                                                         gATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATAGGGGCAGGATTAGGGCGGGAGTTCTCCGTGGTCGCGGAGCCAGGCGGGAGgtttt                                                         >  2:1900186/1‑287 (MQ=255)
                                                                                                                                                                                                                         gATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCCATACGGTTATGACGTCAAACCTGATATGACACCGCGCGAGCCGCGGCGCGTGATTGTGGCATT‑CCAGATGCATTTCCGCCCGCCGCTATATAACGGCGAAGCGGAGGCAGAAACTCAGGCGATTCCCGAAGCATTGCTGGAGAACAACGGGCAGGATTCGCCAGGCAGTTGTCCGTGGTCCCGTAGTCAGGCGGCAGttttt                                                         >  2:1368435/1‑287 (MQ=255)
                                                                                                                                                                                                                             gccAGCGGGTTACCTTGTGCCTTGCCGGGGCGGCGCCCCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                     <  2:1682658/286‑1 (MQ=255)
                                                                                                                                                                                                                             cgcgccGGGTTAACTTTTCCCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                     <  1:1003566/283‑1 (MQ=255)
                                                                                                                                                                                                                                  cGGGTTAACTTTTACCTGGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                <  2:1225025/288‑1 (MQ=255)
                                                                                                                                                                                                                                       tacactttGCCTTGCCGGGCGCGCGCCGCACACACCGGTAGAGACCGCGTCATGGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAACCCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                          <  2:269863/284‑1 (MQ=255)
                                                                                                                                                                                                                                       tAACTTTTCCCTTGCCGGGGGCGCGCCGCACAAACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                          <  2:1704535/289‑1 (MQ=255)
                                                                                                                                                                                                                                       tAACTTTTACCTTGCCGGGCGCGCGCCGCACACCCCGGTAGATACCGCGTCATTGCTGGAGCTGTTGGCGCGCTACGGTTATGCCGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGCTTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTTGTGGATGAATCAGGGCAGGACTAGCGCGGCAGTTTTCCCGGGTCGCGGCGCCAGGCGCGAGGTTtccagatgactttcgt                                           >  2:729505/1‑274 (MQ=255)
                                                                                                                                                                                                                                                   tGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGGTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAcc                              >  1:581485/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    gCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTTCGTGGTCGCGTAGCCAGGGGGCAGGTTTCTCGATACCTTCCTCCAGGGTGATGAACg                             >  2:847445/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 cgcACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                <  1:543358/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cacacaCCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa                                         >  1:1415259/1‑262 (MQ=255)
                                                                                                                                                                                                                                                                   cacaacgcGGTAGATCCCGCGTCAGTGCTGGATCTTGTGGAGCGCAACGGTTATGACGGTAAACCTGATATGACACCGCGCGAGCGGCGGCGCGTGAGTAGGGCACT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct              <  2:1344410/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                        acCGGTAGATACCGCGTCATTGCGGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                 <  1:384182/249‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ccGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTGTTTCGATACCTTCTTCCAGGGTGATGACCGGCTGATAACCTAAACCTTcc         >  2:641173/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                          cGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                     <  1:1197050/243‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gaagttACCGCGTCATTGCTGGGGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgc      <  2:867366/283‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcg     <  1:331820/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCACGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTGTTCCGTGGTCGCGTAGCCAGGCGGCAGGTTTCTCGATACCTTCATCCAGGGTGATGATCGGATGCTGACCTAAGTCTTCCTgcg     >  1:1695221/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                 tACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc  <  1:1872765/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc  <  1:1686657/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
TGGCTGGCAAAAAAGCGCCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGAGTTTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATT‑CCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAC  >  CP042583/768175‑768734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A