Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,813,057 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,813,0570GC99.7% 683.7 / NA 291intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (153/134);  total (156/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,813,0580CG99.3% 678.1 / NA 291intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (154/135);  total (156/135)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,813,0590TA99.3% 688.1 / NA 291intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (154/134);  total (156/135)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

CGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGGCTACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATGCTA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGGCTGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAAGCTGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTACTGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCGCTTTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCC  >  CP042583/1812777‑1813336
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
cGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAa‑tcaat                                                                                                                                                                                                                                                                                 <  2:1206256/289‑1 (MQ=255)
      cGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCa                                                                                                                                                                                                                                                                           <  1:1496535/289‑1 (MQ=255)
                      gACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTc                                                                                                                                                                                                                                                           >  1:191143/1‑289 (MQ=255)
                         cAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCTGCGGTTCATCGACCATTAATATTTCTGGTTGCGTACGTAGCCAGCGACCAATAATCACCTTTTACTGATTACCACCGGAGAGCGAACTCATTTCCGACCGATGACCCGGCGTTTTTACCTGCGT‑CTACTCAAACACACACTggtgacctccttgcct                                                                                                                                                                                                                                                         >  2:1648267/1‑274 (MQ=255)
                           gAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCAACCACTGGCTACCGATTTTCATcc                                                                                                                                                                                                                                                       >  1:947759/1‑288 (MQ=255)
                             aTACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATcc                                                                                                                                                                                                                                                       <  2:1465033/286‑1 (MQ=255)
                                   tCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCgcgagtta                                                                                                                                                                                                                                               >  2:1400467/1‑281 (MQ=255)
                                      gTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATGCGCGAGTTATCCa                                                                                                                                                                                                                                           >  1:1853377/1‑289 (MQ=255)
                                      gTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCa                                                                                                                                                                                                                                           <  1:1743002/289‑1 (MQ=255)
                                      gTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCa                                                                                                                                                                                                                                           >  1:284808/1‑289 (MQ=255)
                                       ttATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAg                                                                                                                                                                                                                                          <  1:1444745/289‑1 (MQ=255)
                                             cTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTAt                                                                                                                                                                                                                                                                   <  2:1032219/258‑1 (MQ=255)
                                             cTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCgcgag                                                                                                                                                                                                                                                  <  1:695430/275‑5 (MQ=255)
                                             cTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCgcgag                                                                                                                                                                                                                                                  >  2:695430/1‑271 (MQ=255)
                                             cTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAAcc                                                                                                                                                                                                                                    >  2:135651/1‑289 (MQ=255)
                                                    cTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCTCGTCGGTTCATCGAGCATTAATATTTCTGGTTCCGTCAGTAGCCATCGACCAATAATCACCTTTTGCTGATTACCACCGGACAGCGAACCACTTTCCGTACGATGACCCGGCGTTTTTACCCGAACCGAA‑TTAACCCCCCACTGGGAATCGCTTTCCACCCCACAGTGATCCAGGAAAACAGCGTTa                                                                                                                                                                                                                             >  2:268384/1‑289 (MQ=255)
                                                    cTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTa                                                                                                                                                                                                                             >  1:1387674/1‑289 (MQ=255)
                                                              tCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTCACCCAACTTTATTTTTGTatt                                                                                                                                                                                                                   >  1:1092532/1‑287 (MQ=255)
                                                                       ataataataATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAatat                                                                                                                                                                                                          >  1:481398/1‑289 (MQ=255)
                                                                       ataataataATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCAATAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTAGCGCTTTTCATACGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAatat                                                                                                                                                                                                          >  1:1170342/1‑289 (MQ=255)
                                                                          ataataatCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTgg                                                                                                                                                                                                       <  1:1276799/289‑1 (MQ=255)
                                                                            aataatCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCAAATATTGGaa                                                                                                                                                                                                     >  2:712007/1‑289 (MQ=255)
                                                                                 tCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTa                                                                                                                                                                                                 >  1:1727922/1‑288 (MQ=255)
                                                                                 tCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAA‑AATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAAGATTGGAAATTaac                                                                                                                                                                                               >  2:65740/1‑288 (MQ=255)
                                                                                  ccccTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaac                                                                                                                                                                                               >  1:1150004/1‑288 (MQ=255)
                                                                                   cccTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacg                                                                                                                                                                                              >  1:1842530/1‑287 (MQ=255)
                                                                                     cTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacgag                                                                                                                                                                                            >  2:1116117/1‑285 (MQ=255)
                                                                                     cTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacgag                                                                                                                                                                                            >  1:39207/1‑285 (MQ=255)
                                                                                      ttGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacgagt                                                                                                                                                                                           >  1:768675/1‑284 (MQ=255)
                                                                                       tGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTCGTTGCGAATATTGGAAATTatcgagtt                                                                                                                                                                                          >  2:1152951/1‑282 (MQ=255)
                                                                                        gCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacgagtta                                                                                                                                                                                         >  2:877617/1‑282 (MQ=255)
                                                                                          cTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacgagttaa                                                                                                                                                                                        <  1:1503555/288‑9 (MQ=255)
                                                                                          cTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaacgagttaa                                                                                                                                                                                        <  1:527142/288‑9 (MQ=255)
                                                                                              cttcGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCa                                                                                                                                                                                   <  1:877617/289‑1 (MQ=255)
                                                                                              cttcGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCa                                                                                                                                                                                   <  1:1400467/289‑1 (MQ=255)
                                                                                                    cAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATc                                                                                                                                                                              <  1:1116117/288‑1 (MQ=255)
                                                                                                       ttCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGa                                                                                                                                                                          >  1:1779768/1‑289 (MQ=255)
                                                                                                       ttCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGa                                                                                                                                                                          >  1:1560210/1‑289 (MQ=255)
                                                                                                       ttCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGa                                                                                                                                                                          >  1:1186431/1‑289 (MQ=255)
                                                                                                       ttCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGa                                                                                                                                                                          >  1:418272/1‑289 (MQ=255)
                                                                                                               ttAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATa                                                                                                                                                                  <  1:1028626/289‑1 (MQ=255)
                                                                                                                      aTAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTccc                                                                                                                                                           >  2:369642/1‑289 (MQ=255)
                                                                                                                                     ccccccggactCATTACCCGGCGTCAGTTCACCGAGCATTAATATGTTTGGTGGCGTTAATAGCCAACCAACAATCATCACCGTTCGCTGATTACCACCGGACATCGAACCAATTTGCGTCCGATGACCCGGCATTTTTACCCGCATCGAA‑TAAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCCACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCCATCTCCAGATAGGCATAAATTCCAGTGGAGCGGCGctcc                                                                                                                                            <  2:675910/279‑1 (MQ=255)
                                                                                                                                                  aTACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTg                                                                                                                               <  1:1829432/289‑1 (MQ=255)
                                                                                                                                                      cgcgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGa                                                                                                                                                                          <  2:1427801/242‑1 (MQ=255)
                                                                                                                                                       gcgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCaaa                                                                                                                           >  1:680418/1‑288 (MQ=255)
                                                                                                                                                        cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTaa                                                                                                                                                                                                <  1:970046/218‑1 (MQ=255)
                                                                                                                                                        cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAAt                                                                                                                          >  1:240053/1‑288 (MQ=255)
                                                                                                                                                        cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAAt                                                                                                                          >  2:743069/1‑288 (MQ=255)
                                                                                                                                                        cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAAt                                                                                                                          >  1:861286/1‑288 (MQ=255)
                                                                                                                                                          ggTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCa                                                                                                                       <  1:1645926/288‑1 (MQ=255)
                                                                                                                                                           gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGct                                                                                                                                              <  2:1851535/265‑1 (MQ=255)
                                                                                                                                                           gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCa                                                                                                                       <  1:712007/288‑1 (MQ=255)
                                                                                                                                                           gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAATTAAAACCAATATCCATATAGGCATAAATTCCCGTTTAGCGGCGCCCCCCAGTTActgtcgcttataccc                                                                                                                      >  2:1581990/1‑275 (MQ=255)
                                                                                                                                                            tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATg                                                                                                                     >  1:1199144/1‑289 (MQ=255)
                                                                                                                                                               gTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGtt                                                                                                                  >  2:503320/1‑289 (MQ=255)
                                                                                                                                                                     cGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGc                                                                                                            <  1:825832/289‑1 (MQ=255)
                                                                                                                                                                          aTTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                       >  2:837311/1‑289 (MQ=255)
                                                                                                                                                                          aTTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                       <  2:191143/289‑1 (MQ=255)
                                                                                                                                                                              atatTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGc                                                                                                   <  2:218753/289‑1 (MQ=255)
                                                                                                                                                                                 tttCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                       <  2:1505166/282‑1 (MQ=255)
                                                                                                                                                                                 tttCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCAtt                                                                                                <  1:322487/289‑1 (MQ=255)
                                                                                                                                                                                   tCTGGTTGCGTTAGTAGCCCGCGACCAATACTCACCTTTTGCTTATTACCACCGGAGAGCGAACCACTTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCGCCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTAt                                                                                              <  1:448656/289‑1 (MQ=255)
                                                                                                                                                                                   tCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTAt                                                                                              >  1:159868/1‑289 (MQ=255)
                                                                                                                                                                                    cTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATg                                                                                             >  1:1228871/1‑289 (MQ=255)
                                                                                                                                                                                     tGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATgg                                                                                            <  2:1316465/289‑1 (MQ=255)
                                                                                                                                                                                      ggTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                       >  1:1614540/1‑277 (MQ=255)
                                                                                                                                                                                          tcGTTAGTGGCCAGGGACCAATAATCACCTTTGCCTGATTACCCCCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTAtt                                                                                       <  2:1748047/288‑1 (MQ=255)
                                                                                                                                                                                          gCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGTTTAtt                                                                                       >  2:966815/1‑289 (MQ=255)
                                                                                                                                                                                          gCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTAtt                                                                                       >  1:1882822/1‑289 (MQ=255)
                                                                                                                                                                                             ttAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGa                                                                                     >  1:136371/1‑288 (MQ=255)
                                                                                                                                                                                                 tagCCAGCCACCAATAATCCCCTTTTGTTGATTACCCCCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGt                                                                                <  2:411392/289‑1 (MQ=255)
                                                                                                                                                                                                  agCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGt                                                                                <  2:566467/288‑1 (MQ=255)
                                                                                                                                                                                                     cAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTg                                                                             <  1:1354234/288‑1 (MQ=255)
                                                                                                                                                                                                      aGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAAt                                                                                                                                                               >  2:438001/1‑205 (MQ=255)
                                                                                                                                                                                                      aGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGcc                                                                           >  2:598797/1‑289 (MQ=255)
                                                                                                                                                                                                      aGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGcc                                                                           <  2:1839129/289‑1 (MQ=255)
                                                                                                                                                                                                        cGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTCTTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGCTAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCCGTGCAAATCCATGGTTTATGGCTTCGTTGTCATTATg                                                                                             >  1:1046740/1‑269 (MQ=255)
                                                                                                                                                                                                         ggcccataatCCCCTTTTTCTGATTACCACCGGAGAGCCAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTAACGCTTTTCCTCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGcc                                                                           <  1:577981/281‑1 (MQ=255)
                                                                                                                                                                                                         gACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTg                                                                        <  2:1779768/289‑1 (MQ=255)
                                                                                                                                                                                                         gACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTg                                                                        <  2:481398/289‑1 (MQ=255)
                                                                                                                                                                                                                aatCACCGTTTGCGGATTACAACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGtt                                                                 <  2:1727922/289‑1 (MQ=255)
                                                                                                                                                                                                                    aCCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCACATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTTCAACGTTATgg                                                             >  2:1078022/1‑289 (MQ=255)
                                                                                                                                                                                                                         ttGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCa                                                        >  1:933356/1‑289 (MQ=255)
                                                                                                                                                                                                                         ttGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCa                                                        >  1:206582/1‑289 (MQ=255)
                                                                                                                                                                                                                         ttGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGATCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTCCAACg                                                                   >  1:1469056/1‑278 (MQ=255)
                                                                                                                                                                                                                                ttACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAgccgatt                                                 >  1:225326/1‑284 (MQ=255)
                                                                                                                                                                                                                                  accaccGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAgccgatttc                                               <  2:787110/289‑8 (MQ=255)
                                                                                                                                                                                                                                         gagagcgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGaa                                        >  1:1316806/9‑289 (MQ=255)
                                                                                                                                                                                                                                          agagcgaaCCAATTTGCGTCCGATGACGCAGCGTGTTTACCCGCATAGAC‑ACTATCACCCAGTGGGTATCGCTTTTCATCCGAGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGGGTTAAAAACAATCTCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGaa                                        <  2:1379390/281‑1 (MQ=255)
                                                                                                                                                                                                                                              cgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGACATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGTCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTAGGGTTATTGATCTGTTTGCCGTGCAACTTTTCGTTGCCAGCCGATTCCTCGCGAATACCa                                   >  2:1492157/4‑289 (MQ=255)
                                                                                                                                                                                                                                              cgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCa                                   >  2:1236875/4‑289 (MQ=255)
                                                                                                                                                                                                                                                 aCCAATTTGCGTCCGATGATCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAAt                                <  1:650917/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   cAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATaa                              <  1:495686/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tGACCCGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAata                 <  2:1882822/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cccGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGct                                                                                                                                              >  2:1935430/1‑160 (MQ=255)
                                                                                                                                                                                                                                                                    cccGGCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGct                                                                                                                                              <  1:1935430/160‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ggCGTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTAc          >  1:1677957/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          gTTTTTACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGtt       <  2:1186431/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               tACCCGCATCGAA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGcc  <  1:838044/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                      
CGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGGCTACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATGCTA‑TCAATCACCCACTGGGTATCGCTTTTCATCCGGCTGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAAGCTGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTACTGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCGCTTTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCC  >  CP042583/1812777‑1813336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A