Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,813,186 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,813,1860AT99.3% 650.9 / NA 276intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (126/148);  minor base G (0/2);  total (126/150)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,813,1870CG98.9% 640.7 / NA 276intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (124/149);  minor base T (2/1);  total (126/150)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,813,1880TA98.5% 638.4 / NA 276intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (125/144);  minor base G (1/3);  total (126/150)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

ACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGGCTACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATGCT‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGGCTGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAAGCTGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTACTGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCGCTTTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAAGCTGACATCGCGAATGCTCGGCTGGCGCAGACTCGTCAGGTTACGTACCTCGAGGATGACTTCGCCCGGTTTGTTTTCTTTGT  >  CP042583/1812906‑1813466
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                       
  ttCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGct                                                                                                                                                                                                                                                                                 <  2:1734549/289‑1 (MQ=255)
  ttCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGct                                                                                                                                                                                                                                                                                 <  1:196036/289‑1 (MQ=255)
    ccccccggactCATTACCCGGCGTCAGTTCACCGAGCATTAATATGTTTGGTGGCGTTAATAGCCAACCAACAATCATCACCGTTCGCTGATTACCACCGGACATCGAACCAATTTGCGTCCGATGACCCGGCATTTTTACCCGCATCGA‑ATAAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCCACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCCATCTCCAGATAGGCATAAATTCCAGTGGAGCGGCGctcc                                                                                                                                                                                                                                                                               <  2:675910/279‑1 (MQ=255)
     gCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGctcc                                                                                                                                                                                                                                                                               >  2:1162782/1‑288 (MQ=255)
                       cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGTGTTTGTAGCCAGCGACCAATAATCACCTTTTGCTGATTATCACCGGAGAGCGTACCAATTTGCGTCCGCTGACCCGGCGTTTTTACACTCA‑GCTAATCAGTCACCCACTGGGTATCGCTTTTCATTTGCGAGTTATCTAGTAATCCAACTTTATTTTTGTAGTTGCGAACATTTGAAATTAACGAGTTATAACCATTCTCCAGATAGGCATAAATCACCGTTTGGCGGCTCTCTTCAGTTAcatgtgtacat                                                                                                                                                                                                                                                             >  2:513063/1‑278 (MQ=255)
                       cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCACTTTGCGTCCGCTGACCCGCCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGACTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGCAAATTAACGAGTTAAAACCAATATCCAGATAGGCTTAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAAt                                                                                                                                                                                                                                                             >  2:1913530/1‑288 (MQ=255)
                       cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAAt                                                                                                                                                                                                                                                             >  2:743069/1‑288 (MQ=255)
                       cgcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAAt                                                                                                                                                                                                                                                             >  1:1489455/1‑288 (MQ=255)
                         cgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCAAAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCa                                                                                                                                                                                                                                                          >  2:492804/1‑289 (MQ=255)
                          gTCGGTTCATCGCGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCATCTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCa                                                                                                                                                                                                                                                          <  1:1648267/288‑1 (MQ=255)
                          gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCa                                                                                                                                                                                                                                                          <  1:1166215/288‑1 (MQ=255)
                          gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCAt                                                                                                                                                                                                                                                         >  2:1900481/1‑289 (MQ=255)
                           tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATg                                                                                                                                                                                                                                                        >  1:1199144/1‑289 (MQ=255)
                                      aGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTTAGTACCACCGGAGAGCGAACCAACTTGCGCCCGATGACCCGGCGTTTTTAACCGCATTGA‑ATCAATCACCAACTGGGTATCGCTGTTCATCCGCGAGTTCTCCAGTAAACCAACATTATTTTTGTAGTTGCGAATATGGGCAATTAAAGAGTTAAATCTAATATCCAGATAGGCGTAAATTCCCGTTGAGATGTGCTCCGCTGTTCACAGAGCGAATCCATGGTTCATGGcgt                                                                                                                                                                                                                                             >  2:1668117/1‑287 (MQ=255)
                                         aTTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                                                                                                                                                          <  2:1842530/289‑1 (MQ=255)
                                          ttAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGtt                                                                                                                                                                                                                                         <  2:1278695/289‑1 (MQ=255)
                                           tAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑g                                                                                                                                                                                                                                       <  1:1638447/289‑1 (MQ=255)
                                             atatTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGc                                                                                                                                                                                                                                     <  2:218753/289‑1 (MQ=255)
                                             atatTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTACCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑gg                                                                                                                                                                                                                                      >  2:824455/1‑288 (MQ=255)
                                               atTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCAt                                                                                                                                                                                                                                   <  1:1599430/289‑1 (MQ=255)
                                                tttCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCAtt                                                                                                                                                                                                                                  <  1:322487/289‑1 (MQ=255)
                                                  tCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTAt                                                                                                                                                                                                                                <  1:1875093/289‑1 (MQ=255)
                                                     ggTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                                                                                                                                                          <  2:1614540/277‑1 (MQ=255)
                                                     ggTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGt                                                                                                                                                                                                                                          >  1:1614540/1‑277 (MQ=255)
                                                      gTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGtt                                                                                                                                                                                                                            >  1:191324/1‑289 (MQ=255)
                                                         gCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGTTTAtt                                                                                                                                                                                                                         >  2:966815/1‑289 (MQ=255)
                                                            ttAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGa                                                                                                                                                                                                                       >  1:136371/1‑288 (MQ=255)
                                                                tagCCAGCCACCAATAATCCCCTTTTGTTGATTACCCCCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGt                                                                                                                                                                                                                  <  2:411392/289‑1 (MQ=255)
                                                                 agCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGt                                                                                                                                                                                                                  <  2:566467/288‑1 (MQ=255)
                                                                   ccAACGACCATTAATCACCTTTTGCTGATTACCACCGGGGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTg                                                                                                                                                                                                               <  2:650625/289‑1 (MQ=255)
                                                                    cAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTg                                                                                                                                                                                                                                                                  <  1:477451/238‑1 (MQ=255)
                                                                    cAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTg                                                                                                                                                                                                               <  1:1354234/288‑1 (MQ=255)
                                                                     aGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGcc                                                                                                                                                                                                             >  2:598797/1‑289 (MQ=255)
                                                                     aGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGcc                                                                                                                                                                                                             >  2:1337131/1‑289 (MQ=255)
                                                                       cGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTCTTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGCTAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCCGTGCAAATCCATGGTTTATGGCTTCGTT‑GTCATTATg                                                                                                                                                                                                                               >  1:1046740/1‑269 (MQ=255)
                                                                        gACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTg                                                                                                                                                                                                          <  1:1814661/289‑1 (MQ=255)
                                                                        gACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTg                                                                                                                                                                                                          <  2:1779768/289‑1 (MQ=255)
                                                                          ccAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCa                                                                                                                                                                                                        >  1:787110/1‑289 (MQ=255)
                                                                                atacccTTTTGCTGGTTACCACCGGAGAGCGAAACAATTTGCGTCCGATGACCCGGCGGTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTa                                                                                                                                                                                                  <  2:1092532/285‑1 (MQ=255)
                                                                                        ttGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCa                                                                                                                                                                                          >  1:206582/1‑289 (MQ=255)
                                                                                         tGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCa                                                                                                                                                                                          >  1:408805/1‑288 (MQ=255)
                                                                                         tGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCa                                                                                                                                                                                          >  2:1130452/1‑288 (MQ=255)
                                                                                                tACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAgccgattt                                                                                                                                                                                  <  1:1452239/289‑7 (MQ=255)
                                                                                                     ccGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTcgc                                                                                                                                                                             <  1:861984/289‑1 (MQ=255)
                                                                                                         agagcgaaCCAATTTGCGTCCGATGACGCAGCGTGTTTACCCGCATAGA‑CACTATCACCCAGTGGGTATCGCTTTTCATCCGAGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGGGTTAAAAACAATCTCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGaa                                                                                                                                                                          <  2:1379390/281‑1 (MQ=255)
                                                                                                          gagcgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAAt                                                                                                                                                                         <  2:767984/282‑1 (MQ=255)
                                                                                                          gagcgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTGTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAAt                                                                                                                                                                         <  1:941368/282‑1 (MQ=255)
                                                                                                           agcgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCGGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATa                                                                                                                                                                        >  2:368386/6‑288 (MQ=255)
                                                                                                             cgaaCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCa                                                                                                                                                                     >  1:971855/4‑289 (MQ=255)
                                                                                                                aCCAATTTGCGTCCGATGATCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAAt                                                                                                                                                                  <  1:650917/289‑1 (MQ=255)
                                                                                                                aCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAAt                                                                                                                                                                  <  2:1489455/289‑1 (MQ=255)
                                                                                                                aCCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGAGCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATCTCTCGCGAATACCAAAt                                                                                                                                                                  >  1:173264/1‑289 (MQ=255)
                                                                                                                        gCGTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGtctc                                                                                                                                                          <  1:68825/289‑1 (MQ=255)
                                                                                                                          gTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTa                                                                                                                                                                                                                           <  2:416015/222‑1 (MQ=255)
                                                                                                                          gTCCGATGACCCGGCGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTa                                                                                                                                                                                                                           >  1:416015/1‑222 (MQ=255)
                                                                                                                                        cGTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGt                                                                                                                                          >  1:1271917/1‑289 (MQ=255)
                                                                                                                                         gTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGtt                                                                                                                                         <  2:694522/289‑1 (MQ=255)
                                                                                                                                         gTTTTTACCCGCATCGA‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGtt                                                                                                                                         <  2:1186431/289‑1 (MQ=255)
                                                                                                                                                     catcgaatcaatcaCCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCccacc                                                                                                                              >  1:1840725/7‑289 (MQ=255)
                                                                                                                                                      atcgaatcaatcaCCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCccacca                                                                                                                             >  2:1764745/6‑289 (MQ=255)
                                                                                                                                                       tcgaatcaatcaCCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAg                                                                                                                            <  1:1540317/285‑1 (MQ=255)
                                                                                                                                                                tcaCCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTcaacaa                                                                                                                                                     >  1:274136/1‑255 (MQ=255)
                                                                                                                                                                tcaCCCACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTC‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAAt                                                                                                                   >  2:243530/1‑289 (MQ=255)
                                                                                                                                                                    ccACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCg                                                                                                               <  2:453079/289‑1 (MQ=255)
                                                                                                                                                                    ccACTGGGTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCg                                                                                                               <  1:503320/289‑1 (MQ=255)
                                                                                                                                                                         gggTATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAt                                                                                                          <  2:1885352/289‑1 (MQ=255)
                                                                                                                                                                            tttagCTTTTCAGCCGCGAGTTCTTCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATCTCCAGATAGGCATAAATTCCCGTTGCGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAtct                                                                                                        <  2:842770/284‑1 (MQ=255)
                                                                                                                                                                            tATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAtct                                                                                                        <  2:1511316/288‑1 (MQ=255)
                                                                                                                                                                            tATCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAtct                                                                                                        <  1:828883/288‑1 (MQ=255)
                                                                                                                                                                             aTCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAtctc                                                                                                       >  1:1249048/1‑288 (MQ=255)
                                                                                                                                                                              tCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTAtt                                                                                                                                                                                                                         <  2:1412101/173‑1 (MQ=255)
                                                                                                                                                                              tCGCTTTTCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTAtt                                                                                                                                                                                                                         >  1:1412101/1‑173 (MQ=255)
                                                                                                                                                                                    ttCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGTCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAGCTCCCATTTAt                                                                                               >  1:438227/1‑289 (MQ=255)
                                                                                                                                                                                    ttCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCa                                                                                                                     <  2:831511/267‑1 (MQ=255)
                                                                                                                                                                                     tCATCCGCGAGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATg                                                                                              <  1:743069/289‑1 (MQ=255)
                                                                                                                                                                                         ccgcgagTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGa                                                                                          <  1:837311/283‑1 (MQ=255)
                                                                                                                                                                                         ccgcgagTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGa                                                                                          <  1:243530/283‑1 (MQ=255)
                                                                                                                                                                                         ccgcgagTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATAACGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGAACTACCCTTTATGCAGa                                                                                          >  1:839726/7‑289 (MQ=255)
                                                                                                                                                                                         ccgcaagTTATACAGTAAACCAACTGTATTTTTGTAGTTGCGAATATTGTAAATTAACGAGTTAAAACCAATATCCAGATAGGAATACATTACCGTGGGGCGGCGCTACTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGa                                                                                          <  2:1853377/283‑1 (MQ=255)
                                                                                                                                                                                         cagcaagTTATCCACTAAACCAACTTTGTTTTTGTAGTTGCGAAATGTGGAAATTAACGAGTTAAAACCAATAGCCAGTTAGTCATGCGTTCCCGTTTCGCGGCGCTCCGCTGTTACCCTGGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACATTATGGTGCCCGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCGGACCGGCAATACCGAGGATCTCCCCTTTCTGCAGa                                                                                          <  2:308237/283‑1 (MQ=255)
                                                                                                                                                                                                               atttttttttTGTAGTTGCGATTATTGGAAATTAACGAGTTAAACCCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAAtcg                                                                    <  2:1228871/287‑3 (MQ=255)
                                                                                                                                                                                                               aCTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGAACAAACGAGACATCGCGAAtcg                                                                    >  1:370526/1‑287 (MQ=255)
                                                                                                                                                                                                                cTTTATTTTTGTAGTTGCGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTcgc                                                                                                                                                                             >  1:61966/1‑183 (MQ=255)
                                                                                                                                                                                                                    gttttttTAGTTGCGCATATTGGAAAGTAACGAGTTAAAAACAGTATCCAGATAGGGATAAATTCCCGTTGAGCGGCCCTCCTCAGTTACCAGTGCCACTACATGGTTTATGGCTTCGTT‑TGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAAtcgacggc                                                               <  2:1840725/288‑8 (MQ=255)
                                                                                                                                                                                                                                 cGAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCCCCAGACCGGCAATACCGAGGATCACCCCTTTATGCATATCAAACGAGCCATCGCGAATCGACGGCGGGCGCAgtgacg                                                  >  1:1442091/1‑284 (MQ=255)
                                                                                                                                                                                                                                  gAATATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGCGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTTGGGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAgtgac                                                   <  1:315628/288‑5 (MQ=255)
                                                                                                                                                                                                                                   aaTATTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCa                                                                                                                                                                                                                                                                <  1:1568632/82‑1 (MQ=255)
                                                                                                                                                                                                                                    atatTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAgtgacgtc                                                <  1:1337131/288‑8 (MQ=255)
                                                                                                                                                                                                                                     tatTGGAAATTAACGAGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAg                                              <  1:1888202/289‑1 (MQ=255)
                                                                                                                                                                                                                                               taacgagTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAGGTTACGTAcc                                    <  2:1447337/283‑1 (MQ=255)
                                                                                                                                                                                                                                               taacgagTTAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAGGTTACGTAcc                                    <  2:1249048/283‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTTGCGAATACCAAAt                                                                                                                                                                  <  1:1708888/156‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttAAAACCAATATCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTTGCGAATACCAAAt                                                                                                                                                                  >  2:1708888/1‑156 (MQ=255)
                                                                                                                                                                                                                                                               atatCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAGGTTACGTACCTCGAGGATGACTTCg                     >  1:175876/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               atatCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAGGTTACGTACCTCGAGGATGACTTCg                     >  1:1863744/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                tatCCAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTTCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAGGTTACGTACCTCGAGGATGACTTCGc                    >  2:918458/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    cAGATAGGCATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCACAGTGACGTCAGGTTACGTACCTCGAGGATGACTTCGCCCGgt               <  1:1130452/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCt                                                                                                   <  2:262294/197‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGATTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCCCGAATCGCCGGCTGGCCCCGTGACGTCAGGTTACGTACCCGGCGGCTGACTCTGCCCGggttgttt         >  2:1047500/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                            cATAAATTCCCGTTGAGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCCGACTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAACGAGACATCGCGAATCGACGGCTGGCGCAGTGACGTCAGGTTACGTACCTCGAGGATGACTTCGCCCGgtttgttt         >  1:1086163/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                       
ACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGGCTACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATGCT‑ATCAATCACCCACTGGGTATCGCTTTTCATCCGGCTGTTATCCAGTAAACCAACTTTATTTTTGTAGTTGCGAATATTGGAAATTAAGCTGTTAAAACCAATATCCAGATAGGCATAAATTCCCGTACTGCGGCGCTCCTCAGTTACCAGTGCAAATCCATGGTTTATGGCTTCGTT‑GGCATTATGGTTATTGATCTGTTTGCCGTGCAACGTTATGGTGCCAGCGCTTTTCTCGCGAATACCAAATAACGTCTCAACAATATCGGTACGTTTCGCCCCCACCAGACCGGCAATACCGAGGATCTCCCCTTTATGCAGATCAAAGCTGACATCGCGAATGCTCGGCTGGCGCAGACTCGTCAGGTTACGTACCTCGAGGATGACTTCGCCCGGTTTGTTTTCTTTGT  >  CP042583/1812906‑1813466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A