Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,814,221 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,814,2210AT99.6% 531.3 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (106/115);  minor base C (1/0);  total (107/115)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,814,2220CG100.0% 523.8 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (107/115);  total (107/115)
*CP0425831,814,2230TA99.1% 517.7 / NA 222intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (105/115);  minor base C (2/0);  total (107/115)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 222 total aligned reads displayed.

ACTCCTGGTGTACCATGCTAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTGCTTTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTACTGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCA  >  CP042583/1813944‑1814498
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                    
 cTCCTGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGACATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTAGTTGATACCGCTCATTTCCAACAAGCATTCCCCGGAGGACGGAATCGTCGAGCTGACCatt                                                                                                                                                                                                                                                                            >  1:734131/1‑288 (MQ=255)
 cTCCTGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCata                                                                                                                                                                                                                                                                            <  1:288264/289‑1 (MQ=255)
     tGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑t                                                                                                                                                                                                                                                                       >  1:504941/1‑289 (MQ=255)
     tGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑t                                                                                                                                                                                                                                                                       >  1:198390/1‑289 (MQ=255)
     tGGTGTACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑t                                                                                                                                                                                                                                                                       >  1:1845390/1‑289 (MQ=255)
      ggTGTACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑tt                                                                                                                                                                                                                                                                      >  2:1101306/1‑289 (MQ=255)
         gtACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTa                                                                                                                                                                                                                                                                   <  1:1015137/289‑1 (MQ=255)
         gtACCATCGAAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTa                                                                                                                                                                                                                                                                   <  1:1002435/289‑1 (MQ=255)
          taccatcgaaaTACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTa                                                                                                                                                                                                                                                                   >  1:1154009/10‑288 (MQ=255)
           accatcgaaaTACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTAcc                                                                                                                                                                                                                                                                 <  1:607208/281‑1 (MQ=255)
             catcgaaaTACCATTTTTCAGGGCTTCATTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCtt                                                                                                                                                                                                                                                               <  2:791898/283‑1 (MQ=255)
               tcgaaaTACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCttgt                                                                                                                                                                                                                                                             >  2:644168/5‑289 (MQ=255)
                  aaaTACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCttgttg                                                                                                                                                                                                                                                           >  1:92627/2‑288 (MQ=255)
                     tACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCa                                                                                                                                                                                                                                                       <  1:1122570/289‑1 (MQ=255)
                     tACCATTTTCCAGGGCTTCTTTTGCAGAATAGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCa                                                                                                                                                                                                                                                       <  2:1275889/289‑1 (MQ=255)
                      aCCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCAt                                                                                                                                                                                                                                                      >  2:1033047/1‑289 (MQ=255)
                          ttttCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACaa                                                                                                                                                                                                                                                  >  1:551061/1‑289 (MQ=255)
                            ttCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTTCCGGAGTCTTGTTGATAAATACCAAACAGGCATTTTAATAATGGCGATGTTCCTTCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTACCGTTATCAAGTGCCTTAACACCAGACAAGGACTTGTTGATACTGCTCATTGCCAACAAGTATTCCCCGGAGGACGGACTCGTTGACCTGACCATACAA‑TTTTACCTTGTTGCCCATACAATa                                                                                                                                                                                                                                                >  2:766264/1‑289 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAggg                                                                                                                                                                                                                                            <  1:1033047/286‑1 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  1:1381892/288‑1 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  1:1553291/288‑1 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  2:1845390/288‑1 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  2:441763/288‑1 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  2:808788/288‑1 (MQ=255)
                                   gCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAAGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAggg                                                                                                                                                                                                                                            <  1:219189/286‑1 (MQ=255)
                                   gCTTCTGTTGCTGAATGGAAATTGATCGCTTTACCCTGTAATAAAATGGTTCCGCAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGGATGGATAGAGTGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  2:1858509/288‑1 (MQ=255)
                                       cttTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACG‑‑GTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAAt                                                                                                                                                                                                                                   >  1:844978/1‑289 (MQ=255)
                                            gCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGa                                                                                                                                                                                                                                <  1:1304012/289‑1 (MQ=255)
                                                  tGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGGCGTTGAGACGACCATATAA‑TTGTACCTTGTTGGCCATCCAAGACGGGAGCAGTACTaagcatca                                                                                                                                                                                                                           >  2:950006/1‑281 (MQ=255)
                                                       aTCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAAt                                                                                                                                                                                                                     <  1:828221/289‑1 (MQ=255)
                                                        tCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATc                                                                                                                                                                                                                    <  2:1787774/289‑1 (MQ=255)
                                                            tccctTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCACGGACAGAATGTGGCCGGCCTTTTAAATTAACGTTATCAAGTGCCTTAACACCCGGAACGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACTGATTCGTTGAGCTGACCTTTTAA‑TTTACCCTTGTCGGCCATACAATATTGGCGCAGTAATAGATTGCGACCAATCAgc                                                                                                                                                                                                                 >  2:484952/4‑287 (MQ=255)
                                                                  tACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTAttt                                                                                                                                                                                                          >  1:1604243/1‑289 (MQ=255)
                                                                      cTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCCAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTg                                                                                                                                                                                                      <  2:1555910/289‑1 (MQ=255)
                                                                       tGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGTCTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGTACTTGTTGATACCGCTCTTTTCCAACAAGTATTCACCGGAGGACGGAGTCGTTGCGCTGACCATCTAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAATAATAGACTGCTCCCAATCAGTCTTATTTCtt                                                                                                                                                                                                       >  2:232445/1‑287 (MQ=255)
                                                                        ggAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAggg                                                                                                                                                                                                                                            <  2:1176825/249‑1 (MQ=255)
                                                                         gAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGCGCGCAGTAATAGACTCAGCCCAATCAGTCTTATTTCTTGCTg                                                                                                                                                                                                   >  2:1161162/1‑289 (MQ=255)
                                                                          aaTAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          >  2:903246/1‑249 (MQ=255)
                                                                          aaTAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          >  2:1321214/1‑249 (MQ=255)
                                                                          aaTAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          <  1:903246/249‑1 (MQ=255)
                                                                                aggttGCCGGAATCTTTTTGATAAATACCAAACAGGACTTTTAATAATGTCGATTTTCCTGCACCGTTTTCACCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAg                                                                                                                                                                                            <  2:1031258/285‑1 (MQ=255)
                                                                                   gTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGGCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTAttt                                                                                                                                                                                                          >  1:1246001/1‑272 (MQ=255)
                                                                                                tgtGATCAATACCAAGCAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATc                                                                                                                                                                            <  2:388531/287‑1 (MQ=255)
                                                                                                   gATAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTAc                                                                                                                                                                         >  1:1417455/1‑289 (MQ=255)
                                                                                                    aTAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGc                                                                                                                                                                                                                                                         >  1:1442846/1‑208 (MQ=255)
                                                                                                    aTAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGc                                                                                                                                                                                                                                                         <  2:65167/208‑1 (MQ=255)
                                                                                                     tAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACg                                                                                                                                                                        <  1:1566103/288‑1 (MQ=255)
                                                                                                     tAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCACTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACg                                                                                                                                                                        <  1:1170051/288‑1 (MQ=255)
                                                                                                                                        tCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGAtt                                                                                                                                    >  1:984695/1‑289 (MQ=255)
                                                                                                                                                ccttttCCCCCATTAATGGATGGGTAGGAAGTGGCCCGGCTTTTAAAATAACGGTTTCCAATGCCTTAAAACCAGGGAAGGACTTGGTGATAACGCCCATTTCCAACAAGTATTCCCCGGAGGAAGGAGTCGTTGAGCTTACCATATAATTTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGt                                                                                                                             <  1:514026/287‑1 (MQ=255)
                                                                                                                                                  ttttCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAgg                                                                                                                                                                                       <  2:1809129/228‑1 (MQ=255)
                                                                                                                                                     tCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTg                                                                                                                        >  1:424359/1‑288 (MQ=255)
                                                                                                                                                       ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCa                                                                                                                     >  1:1759863/1‑289 (MQ=255)
                                                                                                                                                       ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TGTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCa                                                                                                                     <  1:743879/289‑1 (MQ=255)
                                                                                                                                                            ttAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGCAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTCCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCAACGCCAACATAAGGTACGCGGACCACTTTGTCGTCGATTTTCCATTTGGTGCCATCac                                                                                                                 >  2:431501/1‑287 (MQ=255)
                                                                                                                                                            ttAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGc                                                                                                                <  1:1101306/289‑1 (MQ=255)
                                                                                                                                                              aaTGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCg                                                                                                              <  2:536857/289‑1 (MQ=255)
                                                                                                                                                                     ggATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCttt                                                                                                       >  1:583958/1‑289 (MQ=255)
                                                                                                                                                                     ggATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCCGACCACTTGGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGACCCtt                                                                                                        >  2:531880/1‑288 (MQ=255)
                                                                                                                                                                               tgaccGGAATTTTAAATTAACGTTATAAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACG‑‑GTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCa                                                                                            <  2:844978/285‑1 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTTACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTTTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTTGTGCCATTAGCCGCACCTTTACCATCGGCCAg                                                                                           >  2:1578667/1‑289 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAggg                                                                                                                                                                                                                                            >  1:281138/1‑144 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAATGTACGCGGTCCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAg                                                                                           >  2:736582/1‑289 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAg                                                                                           >  1:1108278/1‑289 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAg                                                                                           >  1:264246/1‑289 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAg                                                                                           >  1:1650352/1‑289 (MQ=255)
                                                                                                                                                                                 gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAg                                                                                           >  2:1131747/1‑289 (MQ=255)
                                                                                                                                                                                     gACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGttt                                                                                       >  1:277220/1‑289 (MQ=255)
                                                                                                                                                                                     gACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGttt                                                                                       >  2:616102/1‑289 (MQ=255)
                                                                                                                                                                                     gACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGttt                                                                                       >  1:334810/1‑289 (MQ=255)
                                                                                                                                                                                     gACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGAACCTTTACCATCGGCCAGGttt                                                                                       >  2:1237878/1‑289 (MQ=255)
                                                                                                                                                                                          ttAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGc                                                                                  <  2:285381/289‑1 (MQ=255)
                                                                                                                                                                                                              gCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGcc                                                                                 >  1:450281/1‑270 (MQ=255)
                                                                                                                                                                                                               ccTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTACTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGACAGATCAAAGGTCGCTTTCg                                                              >  1:1750123/1‑288 (MQ=255)
                                                                                                                                                                                                                  tAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGgcg                                                                                                                                                                                                                                          >  2:1480559/1‑113 (MQ=255)
                                                                                                                                                                                                                  tAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTg                                                          <  1:644168/289‑1 (MQ=255)
                                                                                                                                                                                                                  tAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTg                                                          <  1:1250490/289‑1 (MQ=255)
                                                                                                                                                                                                                  tAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTg                                                          <  2:424359/289‑1 (MQ=255)
                                                                                                                                                                                                                       ccAGGAAAGGACTTGTTGCTACCGCTCATTTCCAACACGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATCTAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGgttgt                                                     <  2:342514/289‑1 (MQ=255)
                                                                                                                                                                                                                          ggAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGCGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTAACTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATGTTCCAGTTGGTTGCAGCTGCCGCACCTTCACCATGGGACATGTGTTTCTCCTGTTCAAAGGTTGCTTTCGACTgttctttag                                                  >  2:363865/1‑281 (MQ=255)
                                                                                                                                                                                                                          ggAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGTTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTa                                                   >  2:8900/1‑288 (MQ=255)
                                                                                                                                                                                                                             aaGGACTTGTTGCTACCGCTCATTTCCAACAAGTAGTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCAt                                               <  2:565827/289‑1 (MQ=255)
                                                                                                                                                                                                                             aaGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa                                                <  2:1404450/288‑1 (MQ=255)
                                                                                                                                                                                                                             aaGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCa                                                <  2:504941/288‑1 (MQ=255)
                                                                                                                                                                                                                                       tgATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTa                                      <  1:616160/288‑1 (MQ=255)
                                                                                                                                                                                                                                          tACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTTAGTACGGt                                  >  1:1883497/1‑289 (MQ=255)
                                                                                                                                                                                                                                           aCCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATC‑ACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACg                                    >  2:1637518/1‑285 (MQ=255)
                                                                                                                                                                                                                                           aCCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATC‑ACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACg                                    <  1:1637518/285‑1 (MQ=255)
                                                                                                                                                                                                                                               cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAggg                                                                                                                                                                                                                                            >  2:171202/1‑82 (MQ=255)
                                                                                                                                                                                                                                               cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGccc                              >  2:1154513/1‑288 (MQ=255)
                                                                                                                                                                                                                                               cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGccc                              >  1:1310990/1‑288 (MQ=255)
                                                                                                                                                                                                                                               cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGATGccc                              >  1:1490763/1‑288 (MQ=255)
                                                                                                                                                                                                                                               cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTCCCCCTGGTTGTTAGCTTCGTTCAGTACGGTGccc                              >  2:1438082/1‑288 (MQ=255)
                                                                                                                                                                                                                                               cTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TGTTACCTTGTTGGCCATACAATAAGTGCGCAGTAATAGACTGCGCCCAATCAGTCTTTTTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGccc                              >  1:68397/1‑288 (MQ=255)
                                                                                                                                                                                                                                                tCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCg                             >  1:1190509/1‑288 (MQ=255)
                                                                                                                                                                                                                                                tCATTGCCAACATGTATTCCCCGGGGGCCGGAGTCGTTGAGCTGCCCATAGAA‑TTTTACCTGTTTGGCCATACAATAAGGGCGCAGTAATATACTGCGCCCAATCATTCTTATTTCTTGCGGAATTCCGCCAGGTTGTCTTCATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGGGCCATCAGCCGCACCTTTGCCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGc                            <  2:92627/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        aacaaGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCAc                    <  2:298565/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                ttCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTc            >  2:626246/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           aCGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCCGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCAccagcgcca  <  1:1157141/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aCGGAGTCGTTGAGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCCTCGTTCAGTACGGTGCCCGCCAGTGCACGGGTTTTCAccggcgcca  >  2:1358339/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                    
ACTCCTGGTGTACCATGCTAATACCATTTTCCAGGGCTTCTTTTGCAGAATGGAAATCGATCTCTTTACCCTGGAATAAAATGGTGCCGGAGTCTTTTTGATAAATACCAAACAGGCATTTTAATAATGTGCTTTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTACTGCTGACCATATAA‑TTTTACCTTGTTGGCCATACAATAAGGGCGCAGTAATAGACTGCGCCCAATCAGTCTTATTTCTTGCTGAATTCAGCCAGGTTGTCTTTATCTACGCCAACATAAGGTACGCGGACCACTTTGTTGTCGATTTTCCAGTTGGTGCCATCAGCCGCACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCA  >  CP042583/1813944‑1814498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A