Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,957,883 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,957,8830AT99.6% 569.8 / NA 240intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (97/142);  minor base G (1/0);  total (98/142)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,957,8840GC99.2% 549.2 / NA 240intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (98/139);  minor base A (0/2);  total (98/142)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
*CP0425831,957,8850TA99.6% 563.5 / NA 240intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (97/142);  minor base C (1/0);  total (98/142)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGAGCGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTAGCCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGAGTTCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATT  >  CP042583/1957603‑1958163
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
      aaCAGGCGGGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTa                                                                                                                                                                                                                                                                            <  1:987470/289‑1 (MQ=255)
       aCAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTAt                                                                                                                                                                                                                                                                           <  1:1911002/289‑1 (MQ=255)
         aGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCt                                                                                                                                                                                                                                                                         >  1:1577957/1‑289 (MQ=255)
           gCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCAGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCATGTAAAATGGGCACTGTTGTCATCTCAGGAGTTATCTGt                                                                                                                                                                                                                                                                       >  2:1349293/1‑289 (MQ=255)
            cgggCAGCTGGTCAGCGATGGGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCCGTGCATGCAGCTTCGCGTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTa                                                                                                                                                                                                                                                                            <  2:199812/280‑1 (MQ=255)
            cGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGt                                                                                                                                                                                                                                                                       <  1:918099/288‑1 (MQ=255)
               gCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCCGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGCATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGCGGAGAC‑TTTGTTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCTTCAGGTAAAAGCGGCATTGTTGTGATCTCAGGATTTATCTGTTTAt                                                                                                                                                                                                                                                                   <  1:544421/289‑1 (MQ=255)
                   cTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCt                                                                                                                                                                                                                                                                <  2:569714/288‑1 (MQ=255)
                   cTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCt                                                                                                                                                                                                                                                                <  1:622847/288‑1 (MQ=255)
                       tcgccttttggtcggaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGa                                                                                                                                                                                                                                                            <  2:1577957/274‑1 (MQ=255)
                       tCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGa                                                                                                                                                                                                                                                            <  1:27101/288‑1 (MQ=255)
                           cTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGaa                                                                                                                                                                                                                                                           >  1:1143637/1‑285 (MQ=255)
                             atgcgtcggaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                                                                                                                                                     <  2:770728/281‑1 (MQ=255)
                             atgcgtcggaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                                                                                                                                                     >  2:716937/9‑289 (MQ=255)
                             atgcgtcggaagaaCCGCCCGTATGTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                                                                                                                                                     <  2:1355325/281‑1 (MQ=255)
                                ggtgggagtaaCCGGCGTTCGTTGGTGGGGAGGAAATGGGTGGGCCGGTGCAGGCAGCTTGGCTTGGGCGTCCAGTGAAATTAATGATGGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCa                                                                                                                                                                                                                                                    <  2:361644/278‑1 (MQ=255)
                                  tcggaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGt                                                                                                                                                                                                                                                >  1:1405781/4‑289 (MQ=255)
                                     gaagaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAACGCGGCATTGTTTGCATCTCAGGAATGATCTGTTTATTCTATGAATACCCCAGGGTTTa                                                                                                                                                                                                                                             >  2:14876/1‑289 (MQ=255)
                                       agaaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTAt                                                                                                                                                                                                                                            <  1:1427791/288‑1 (MQ=255)
                                          aCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTcc                                                                                                                                                                                                                                         <  1:458201/288‑1 (MQ=255)
                                          aCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAAGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTcc                                                                                                                                                                                                                                         <  2:1667049/288‑1 (MQ=255)
                                            cGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCgg                                                                                                                                                                                                                                      >  2:299339/1‑289 (MQ=255)
                                                             gATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGac                                                                                                                                                                                                                      <  1:693559/288‑1 (MQ=255)
                                                             gATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGac                                                                                                                                                                                                                      <  2:1277810/288‑1 (MQ=255)
                                                               tGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGaccac                                                                                                                                                                                                                   <  1:798640/289‑1 (MQ=255)
                                                                           cgcgGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTTAATTATTg                                                                                                                                                                                                        >  2:599301/1‑288 (MQ=255)
                                                                             cgGTGCGTGCAGCGTCGCTTGCGCGTCCAGTGCAATTAATGTACGCACTTGAGATGATTGGCTATTCCGACTCTTCGCCTGGAAGCCAGAATGACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCTGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGTATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGc                                                                                                                                                                                                      <  2:305972/288‑1 (MQ=255)
                                                                              ggTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCaa                                                                                                                                                                                                    <  1:1234090/289‑1 (MQ=255)
                                                                                          ttcgcTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATTTGTTTATTCTATGAATACCCCCGGGTTTATTCCCGGTATTGATTTCTCTGACCAACCGAATTATTGGCAACTCGATCTTct                                                                                                                                                                                         >  2:1905792/5‑287 (MQ=255)
                                                                                          ttcgcTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTcc                                                                                                                                                                                         <  2:1439528/284‑1 (MQ=255)
                                                                                            cgcTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGTCACCCTGAATTATTGTCAACACGATATTctct                                                                                                                                                                                       >  2:576348/3‑285 (MQ=255)
                                                                                             gattgggagtgctgTGAAATTAAGTATAGCCCTGGAGATGATTGGCTATTCCGACTCTGCGCCTGGAAGCCAGAATTCCCCTTCCCCGGCAATGTCCTGGCTTTATCCCGCTCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTAAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGcc                                                                                                                                                                                     <  2:1094908/276‑1 (MQ=255)
                                                                                              cTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGTCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTCTCTGTTTATTCTATTAATACCACAGGGTTTATTCCCGGTATTTATTTTTCTGACCTCCTGCATTATTGGCAACACGATATTCCCCCCa                                                                                                                                                                                    >  2:1049480/1‑289 (MQ=255)
                                                                                                             aaaTTAATGGTAGCGCTGGAGTGGATTGGCTCTTTCCACTCTGCGCCGGGAAGCCAGATGTACCCTTACCCGGCAAGGGCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTTTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGaca                                                                                                                                                                     <  2:436033/289‑1 (MQ=255)
                                                                                                             aaaTTAATGATGGCATTGGGGAGGATTGGCTGTTCAGACTCTGCGCCTGAAAGCAGGATTTCCCCTTCCCCGGCAATGGCCTGGCTTTCTCCCGAGCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGaca                                                                                                                                                                     <  2:963588/289‑1 (MQ=255)
                                                                                                              aaTTATTGTTAGCACTGGGGATGTTTGGCTTTTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATTTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGacac                                                                                                                                                                    <  2:884212/289‑1 (MQ=255)
                                                                                                                ttAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCt                                                                                                                                                                                                                 >  2:1523139/1‑242 (MQ=255)
                                                                                                                       ttgaccgtgagaTGTGTGGCTATTCCGAGTGTGCGCCTGGAAGCCAGAATTACCCTGACCCGGCATGGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTAATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTa                                                                                                                                                           <  2:1477165/281‑1 (MQ=255)
                                                                                                                       tAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGCAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGGTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCAAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGACTTATTGGAAACAGGATATTCCCGCCATAATGATTACGTCCACCGCTTTTTa                                                                                                                                                           >  2:263152/1‑289 (MQ=255)
                                                                                                                       tAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTa                                                                                                                                                           <  1:1558594/289‑1 (MQ=255)
                                                                                                                            cTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCa                                                                                                                                                                                    >  1:208905/1‑259 (MQ=255)
                                                                                                                            cTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGta                                                                                                                                                      <  1:449280/289‑1 (MQ=255)
                                                                                                                            cTGGAGATGATTGGCTATTACGACTCGTCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGta                                                                                                                                                      <  1:1127410/289‑1 (MQ=255)
                                                                                                                               gagaTGATTGGCTATTACGAGTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCTTGGCTTTATCCCGAACGGGGAGAC‑TGTATTGCCGTGGTCGGCAGAATACATGATATCAACTCCGTTCGTCAGGTAAAATCGGGATTGTTGTCATCTCAGGCTTTATCTTTTTATTCTATGAATACACTAGGGTTAATTCCCGGTTTTGATTTCTCTGACTACTTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACGTCTTTTTAttctcata                                                                                                                                                   >  2:845416/1‑282 (MQ=255)
                                                                                                                                atagttgtggattttACGTGTCTGAGCCGGGAAGCGAGAATTACCCTTACGCGGAAAGGTCCTCGCGTTATCCCGGTCGGGGGGAC‑TCTATTGCCGTGTTCGGCAGAATACAGGAGATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTAAGGTTTTATCTGTTTATTCTTGGAATACCCCGGCGTTTATTCCCGGTAGTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaata                                                                                                                                                   <  2:814510/275‑1 (MQ=255)
                                                                                                                                  atgatTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGGAACATGATATTCACGCCATATTGATTAATGACACCGCTTTTTATTGTAATAAAc                                                                                                                                                >  2:1195478/1‑289 (MQ=255)
                                                                                                                                   tgatTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAAc                                                                                                                                                <  1:580656/288‑1 (MQ=255)
                                                                                                                                    gatTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACACCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTTGCAACACGTTATTCACGGCATAATGATTACTGACACCGCTTTTTATTGTAAGTAACaa                                                                                                                                              >  1:897040/1‑289 (MQ=255)
                                                                                                                                       tGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATa                                                                                                                                            >  1:1027282/1‑288 (MQ=255)
                                                                                                                                         gCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGACTTATTGGCAACACGATATTCCCGCCATAATTCTTACTGACACCGCTTTTTCTCGTAATAAACCCTAcc                                                                                                                                          >  1:838621/1‑288 (MQ=255)
                                                                                                                                         gCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACaa                                                                                                                                              <  1:970431/284‑1 (MQ=255)
                                                                                                                                           tATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACt                                                                                                                                       <  1:124874/289‑1 (MQ=255)
                                                                                                                                                     ctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGa                                                                                                                             >  2:1722142/1‑289 (MQ=255)
                                                                                                                                                     ctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGa                                                                                                                             >  1:351091/1‑289 (MQ=255)
                                                                                                                                                        cgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCACTTTTTATCGTAATAAACAATACCACTTGCCCGGttcta                                                                                                                           >  2:1020333/1‑284 (MQ=255)
                                                                                                                                                                                acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCg                                                                                                                                                                                                                                       >  2:1819288/1‑156 (MQ=255)
                                                                                                                                                                                acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCg                                                                                                                                                                                                                                       <  1:1819288/156‑1 (MQ=255)
                                                                                                                                                                                acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGca                                                                                                                      <  1:1697839/269‑1 (MQ=255)
                                                                                                                                                                                acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGaa                                                                                                  >  1:1461986/1‑289 (MQ=255)
                                                                                                                                                                                acccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGaa                                                                                                  <  1:14876/289‑1 (MQ=255)
                                                                                                                                                                                 cccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGaaa                                                                                                 <  1:377265/289‑1 (MQ=255)
                                                                                                                                                                                 cccGGCAATGTCCTGCCTTTATCCCGATCGGGGAGCT‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGaaa                                                                                                 <  1:411738/289‑1 (MQ=255)
                                                                                                                                                                                       aaggTCCTGGCTTTATCCTGATGGGGGGGAG‑TTGTTTGCGGTGGTCGGCAGAAAACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAGCACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCt                                                                                           <  2:1405781/286‑1 (MQ=255)
                                                                                                                                                                                       aaTGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAATGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCt                                                                                           <  1:1195478/289‑1 (MQ=255)
                                                                                                                                                                                           tcgtGGCTTTATCCCGTTCGGGGTGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAAAGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAg                                                                                        <  2:817763/285‑1 (MQ=255)
                                                                                                                                                                                           tCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTg                                                                                                                                     >  1:1082288/1‑243 (MQ=255)
                                                                                                                                                                                           tCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTc                                                                                          >  1:722164/1‑286 (MQ=255)
                                                                                                                                                                                                  tttacccagttgggggAGAC‑TTTATTGCCGTGTTCGGCAGATTACAGGATATCAACGCTGTTCGTCAGGTAAAAGCGGCATTGTTTTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGa                                                                                <  2:1169986/277‑1 (MQ=255)
                                                                                                                                                                                                  tttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGa                                                                                <  1:1563086/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCTGTTGGGGGTGAG‑TTTAGTGCCGGGGTCGGCAGAATACAGTAGTACAGATCCGTTCGGCAGGTAAAGGCGTGATTGTTGTCATCTCAGAAGTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCTGGATTGATTTATTTGACCACCTTAATTATGGGCAACACAATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  2:1107679/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGGTCGGGGGGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGAAATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  2:130406/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  1:1758809/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  1:1722142/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  1:1546960/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  1:1133817/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  1:303958/289‑1 (MQ=255)
                                                                                                                                                                                                   ttATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  1:748869/289‑1 (MQ=255)
                                                                                                                                                                                                    ttgtccGATCGGGGGGTCTTTTATTGCGGTGTGCGGCCGAATACAGGCTTTCAACGTTGTTCGTCAGGTAAAAGAGGCATTTTTGTCATCTAGGCATTTATCTGTTTATTCTAAGAATACCCCAGGTTTTATTCCGGGGATTGATTTTCCAGCCCACCTGAATTATTTGCAACAGTATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGAt                                                                               <  2:450855/285‑1 (MQ=255)
                                                                                                                                                                                                     aTCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATgg                                                                             >  1:1803516/1‑289 (MQ=255)
                                                                                                                                                                                                     aTCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATgg                                                                             >  1:130406/1‑289 (MQ=255)
                                                                                                                                                                                                     aTCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATgg                                                                             >  1:81022/1‑289 (MQ=255)
                                                                                                                                                                                                          gATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGcaga                                                                                                                    >  1:1002500/1‑245 (MQ=255)
                                                                                                                                                                                                           aTCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTtat                                                                       >  1:1772447/1‑289 (MQ=255)
                                                                                                                                                                                                             cGGGGAGAC‑TTTGTTGCCGTGTTCGGCAATATGCAGGTTATCAACGCCGTTCGTACGGTAAAAGCTGCATTGTTGTCATCTCAGTATTTTTTTTTTTATTCTATGAATACCCCAGGGTTTATTCCCGTTATTGATTTCTCTGACCACCTGAATTATTGGAAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATaa                                                                     <  2:1241974/289‑1 (MQ=255)
                                                                                                                                                                                                             cGGGGAGAC‑TTTATTGCCGTGGTCGGAAGAATACAGGTTATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATaa                                                                     <  1:1251528/289‑1 (MQ=255)
                                                                                                                                                                                                                 gggaC‑TTTATTGACGTGGTAGGCCGTATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTGTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCACCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACtt                                                                  <  2:33603/286‑1 (MQ=255)
                                                                                                                                                                                                                   gaC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTg                                                                <  1:571513/288‑1 (MQ=255)
                                                                                                                                                                                                                        ttATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTtata                                                           <  1:846700/289‑1 (MQ=255)
                                                                                                                                                                                                                           gtGCCGTGGTGGGAAGAATACAGGTGGTAAGCGCCGTTCGGCAGGTAAAAGAGGCATTGTTGTCATTTCAGGTTTTATCTGTTTAGTCTATGAATACCCCAGGGTTTATTCCCGTTTTTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATacaa                                                        <  2:1777933/288‑1 (MQ=255)
                                                                                                                                                                                                                                        cAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTcc                                                                                                                                                                                         >  1:1927531/1‑147 (MQ=255)
                                                                                                                                                                                                                                                       cAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCg                            >  2:28611/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          cGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTaaa                                                 >  1:1579262/1‑265 (MQ=255)
                                                                                                                                                                                                                                                            ccGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTg                                                                                                                              <  2:702407/186‑1 (MQ=255)
                                                                                                                                                                                                                                                             cGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACATCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGcag                                                                                                                     <  1:164443/194‑1 (MQ=255)
                                                                                                                                                                                                                                                             cGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACATCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGcag                                                                                                                     >  2:164443/1‑194 (MQ=255)
                                                                                                                                                                                                                                                              gTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTa                                                                                                                                                           >  2:1466652/1‑155 (MQ=255)
                                                                                                                                                                                                                                                                        tAAAAGCGGCATTTTTGTCATCTGATGTTTTCTTTGTTTATTCTTTGAATACCCCAGGTTTTTTTCCCGGTTTTAATTTCTCTGAACACCTGAATTATTGGAAACCCGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGAc           <  2:1554021/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATgg                                                                             <  1:972443/214‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATgg                                                                             >  2:972443/1‑214 (MQ=255)
                                                                                                                                                                                                                                                                                 cATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACatt  >  2:367340/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
ACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGAGCGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTAGCCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGAC‑TTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGAGTTCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATT  >  CP042583/1957603‑1958163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A