Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,977,453 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,977,4530AT98.9% 635.0 / NA 272intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (129/138);  minor base C (2/1);  total (133/139)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,977,4540CG98.9% 630.7 / NA 272intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (132/137);  minor base T (1/2);  total (133/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,977,4550TA100.0% 642.5 / NA 272intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (133/139);  total (133/139)

Only 100 of 272 total aligned reads displayed.

CTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTGCTGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGGCTGGTACGCAGGTTCAATGCCTTAAACGCACTAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACGCTACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCACTAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAAACTACTGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAG  >  CP042583/1977173‑1977734
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                        
cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑g                                                                                                                                                                                                                                                                                   <  1:743322/289‑1 (MQ=255)
  ccccccAGCACAGCCAGCTCCGGGCTCCGCATCTCAACCGGCCTTGCCGTCCCCACAAGACCGGCTTTACCACGCGAACGAACGGCCGGAAGCGTAGAGGCGACATACGCCAGGCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GAt                                                                                                                                                                                                                                                                                 <  2:1489935/284‑1 (MQ=255)
  ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GAt                                                                                                                                                                                                                                                                                 <  1:135185/289‑1 (MQ=255)
     cTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACCAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATggg                                                                                                                                                                                                                                                                              >  1:818028/1‑289 (MQ=255)
     cTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGAACGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATggg                                                                                                                                                                                                                                                                              >  2:1691716/1‑289 (MQ=255)
        ggCACACCCAGGCCCGGGCTCATCATGCCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGggtgg                                                                                                                                                                                                                                                                           <  2:1831869/288‑1 (MQ=255)
          cacaCGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCCCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGGGTGTGGGCGTGGTACGCAGGGTCACTTCCTTAAAGGCTGAAGGGG‑GAGGGGTGGtt                                                                                                                                                                                                                                                                         >  2:1661024/1‑289 (MQ=255)
           accggCAGGTCCGGGCCCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTa                                                                                                                                                                                                                                                                        <  2:1256292/285‑1 (MQ=255)
           acccGCAGGGCCGGGCTCATACTCTCAACCGGCCTTGCCGGACCGCCAAAACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGggtggt                                                                                                                                                                                                                                                                          <  2:1338118/284‑1 (MQ=255)
           acacGCAGGTCCGGGCTCATCACCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTa                                                                                                                                                                                                                                                                        <  2:721666/289‑1 (MQ=255)
               gCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATTTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTAACa                                                                                                                                                                                                                                                                     >  1:1383517/1‑288 (MQ=255)
               gCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCa                                                                                                                                                                                                                                                                     >  1:236054/1‑288 (MQ=255)
                cAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGGGTGTTGGCGAGGTACGCAGGTTCAATGCCGTAAACGCTGAAGGACGGATGGGT‑GTTACCAt                                                                                                                                                                                                                                                                    >  2:1797128/1‑288 (MQ=255)
                    tCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGCGGCGACATAAGCCAGTCGCCATGGAAAATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGACGGATCGGCTCGCCTTTTTGCAATATGTATTACGCTGTCCAGTATCAGTGTGTTGGCGAGGTACGCAGGTTCAATTACTTAAACGCTGAAGTAC‑GATGGGTGGTTACCATCcaa                                                                                                                                                                                                                                                                >  1:1944785/1‑288 (MQ=255)
                                         ggCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCa                                                                                                                                                                                                                                          >  1:851968/1‑289 (MQ=255)
                                           ccTGGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCAt                                                                                                                                                                                                                                         <  1:666401/288‑1 (MQ=255)
                                            cTTGCCGTCACGCCCAGACCGGCTGTCACTGCCACACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGAACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCAttt                                                                                                                                                                                                                                       <  2:27577/289‑1 (MQ=255)
                                            cTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCAttt                                                                                                                                                                                                                                       >  2:572101/1‑289 (MQ=255)
                                              tGCCGTCACGCCAAGACCTGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCt                                                                                                                                                                                                                                     <  2:1353327/289‑1 (MQ=255)
                                              tGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCt                                                                                                                                                                                                                                     <  1:694894/289‑1 (MQ=255)
                                               gCCGTCACGCCAAGACCGGCTTTCCCTGCCGCACGCACGGCCGGCAGCGTCGAGGCGACCTACGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGACCGCACCCTACCGCGAAACGGGCTAGGATCATCCAGCAATACAAGCGGGATCGGCCCGCCTTTTTGCCATAAGTATTCCGCCGCGCAGTACCAGCGCGTTGGCCAGGTACGCAGGTTCAATGCCGTAATCGCAGAACGAC‑GCTGGGTCGGTCCCATCCAAACACCCTCCTGGCATTCCAGCATTTCt                                                                                                                                                                                                                                     >  2:1825137/1‑288 (MQ=255)
                                                    cACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCata                                                                                                                                                                                                                               <  1:1443211/289‑1 (MQ=255)
                                                           aGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCAt                                                                                                                                                                                                                        >  1:1303890/1‑289 (MQ=255)
                                                                 gCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGtt                                                                                                                                                                                                                  >  2:910379/1‑289 (MQ=255)
                                                                         tGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCgg                                                                                                                                                                                                          <  1:1123902/289‑1 (MQ=255)
                                                                                   aCGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑g                                                                                                                                                                                                                                                                                   >  2:1392949/1‑206 (MQ=255)
                                                                                   aCGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GCTGGGTGGTTACAATCAAATCCACTTCATGGGATTCCAGCATTTCTGCATTATAGGCATTCGGTTTAACGCGGACACCCCgc                                                                                                                                                                                                 >  2:173419/1‑288 (MQ=255)
                                                                                       gcagatattccatGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTGCGTTTAACGCGGACATCCAGCGCTaa                                                                                                                                                                                            >  2:1434800/13‑289 (MQ=255)
                                                                                               gtcgaggCGACATAAGCCATTCGCCATTGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGAt                                                                                                                                                                                    <  1:310313/284‑1 (MQ=255)
                                                                                                           tacgCCAGCCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGACTTTTTGCAATATGTAGTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAAccgaactca                                                                                                                                                                        <  2:1635176/286‑9 (MQ=255)
                                                                                                           tAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAAccgaactca                                                                                                                                                                        <  1:1076764/289‑9 (MQ=255)
                                                                                                                   tCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGAttt                                                                                                                                                                >  1:1102785/1‑289 (MQ=255)
                                                                                                                    cGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTa                                                                                                                                                               >  2:681768/1‑289 (MQ=255)
                                                                                                                         tGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAgg                                                                                                                                                          <  1:1653497/289‑1 (MQ=255)
                                                                                                                         tGGAATATATCCTGTAGTAGGCGTCGTCACAACTCAATCGCCAAACGCGCTAGGATCGTCCATCGATTCACGAGTGTTCGGCTCGACTTTTTTAGAGATGTAGTCCACTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GGTGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACGCACGCGATTTAACAgg                                                                                                                                                          <  2:1048883/289‑1 (MQ=255)
                                                                                                                         tGGAAGATCTGCTTTATTAAGCGTCGCCAGCACCATAGCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAGCTCACGCGATTTAACAgg                                                                                                                                                          <  1:762989/289‑1 (MQ=255)
                                                                                                                         tGGAAGATCGGCTTTCTTAAGGGTGGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAgg                                                                                                                                                          <  2:1882209/289‑1 (MQ=255)
                                                                                                                          ggAAGATGTGCTTTATTAGACGTCGCCAGCACCATATCGAGAAACGGGCTAGGATCATCCCGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTAGTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGa                                                                                                                                                         <  2:14432/289‑1 (MQ=255)
                                                                                                                           gAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGa                                                                                                                                                         <  2:1640371/288‑1 (MQ=255)
                                                                                                                                tCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTa                                                                                                                                                   >  1:530189/1‑289 (MQ=255)
                                                                                                                                 cTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTaa                                                                                                                                                  >  1:606690/1‑289 (MQ=255)
                                                                                                                                 cTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTaa                                                                                                                                                  >  1:1894672/1‑289 (MQ=255)
                                                                                                                                           aagggccGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAGGTGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCAGCAAAGCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCGAGCGCTAATTTCGGATAAACCGAACTCCCGCGATTTAACAGGAAAGGTAAGATCGTATCg                                                                                                                                        <  1:1825137/283‑1 (MQ=255)
                                                                                                                                              cGTCGCCAGCACCATCTCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAgg                                                                                                                                                          <  2:1728948/268‑1 (MQ=255)
                                                                                                                                                        aCCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCC‑TTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATtcatctga                                                                                                                          <  2:817501/289‑4 (MQ=255)
                                                                                                                                                           atatCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATtcatctgaag                                                                                                                        <  1:1797128/289‑6 (MQ=255)
                                                                                                                                                           atatCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATtcatctgaag                                                                                                                        <  1:703236/289‑6 (MQ=255)
                                                                                                                                                                gcgAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCg                                                                                                                   <  2:1622093/289‑1 (MQ=255)
                                                                                                                                                                gcgAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCg                                                                                                                   <  1:803487/289‑1 (MQ=255)
                                                                                                                                                                          cTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTaa                                                                                                                                                                                                               >  2:114371/1‑187 (MQ=255)
                                                                                                                                                                          cTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACAGATAGTTAAt                                                                                                          <  1:157232/288‑1 (MQ=255)
                                                                                                                                                                           tAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATa                                                                                                         <  1:630229/288‑1 (MQ=255)
                                                                                                                                                                           tAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATa                                                                                                         <  1:387885/288‑1 (MQ=255)
                                                                                                                                                                               atcatcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGcc                                                                                                     <  1:288111/288‑1 (MQ=255)
                                                                                                                                                                                 catcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATc                                                                                                                                                                                                     <  1:556041/190‑1 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGCTTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGACAGGCAAGATCGTATCGGCAGATTCATCGGAACCCACGATAGTTAATACGCCCTGaa                                                                                                >  2:645255/1‑289 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAAccgaa                                                                                                                                                                            <  2:1212757/213‑5 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACTCGATTTAACAGGAAAGGTAAGAACGTATCGGCAGATTCATCTGAAGCACCGCTAGTTAATACGCACTGaa                                                                                                >  2:1921471/1‑289 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGaa                                                                                                >  1:517413/1‑289 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGaa                                                                                                >  1:1556990/1‑289 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGaa                                                                                                >  1:1542581/1‑289 (MQ=255)
                                                                                                                                                                                   tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGAACGTATCGGCAGATTCATCGGAAGCACAGAAAGTTAATACGACCTGaa                                                                                                >  2:346660/1‑289 (MQ=255)
                                                                                                                                                                                     cAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGa                                                                                              <  2:236054/289‑1 (MQ=255)
                                                                                                                                                                                           tACAAGATGGATCGGCTCGCCTTTTTGCAATATGTAGTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTg                                                                                        <  2:543679/289‑1 (MQ=255)
                                                                                                                                                                                           tACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTg                                                                                        <  1:1636564/289‑1 (MQ=255)
                                                                                                                                                                                           tACAAGAGGGATCGGCTCGACTTTTTGCAATATGTAGTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTg                                                                                        <  1:1728962/289‑1 (MQ=255)
                                                                                                                                                                                             cAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTAACGCGATTTAACAgg                                                                                                                                                          >  2:316354/1‑221 (MQ=255)
                                                                                                                                                                                              aaGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAgg                                                                                                                                                          >  1:1059353/1‑220 (MQ=255)
                                                                                                                                                                                                gagGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACAt                                                                                   >  1:572210/1‑289 (MQ=255)
                                                                                                                                                                                                   ggATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGa                                                                                                                                   <  2:1081965/238‑1 (MQ=255)
                                                                                                                                                                                                     aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGACGCACCGAt                                                                                                                 >  2:1567941/1‑254 (MQ=255)
                                                                                                                                                                                                     aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATa                                                                                                         >  1:259236/1‑262 (MQ=255)
                                                                                                                                                                                                      tCGGGTCGCCTCTTTGCAATCTGGATGCTGCTGATGACGACCAGTGTGTTGGGGAGGTACGCAGGTTCAATGGCTTAAGCGCTGATGGAC‑GATGGGTGGTTACCATCAAATCCACGTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACAGCCAGAGGTAATTTCGGATAAACCGAGCTCACGAGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTaatg                                                                              <  2:1552058/288‑3 (MQ=255)
                                                                                                                                                                                                      tCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTaatg                                                                              <  1:122175/288‑3 (MQ=255)
                                                                                                                                                                                                                         tatGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTa                                                          >  2:1611680/1‑289 (MQ=255)
                                                                                                                                                                                                                          atGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaa                                                         <  1:1691716/289‑1 (MQ=255)
                                                                                                                                                                                                                                tCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGaa                                                                                      <  1:1702562/254‑1 (MQ=255)
                                                                                                                                                                                                                                tCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCa                                                   >  2:126854/1‑289 (MQ=255)
                                                                                                                                                                                                                                   gCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGa                                                <  1:1207468/289‑1 (MQ=255)
                                                                                                                                                                                                                                    cTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAt                                               >  1:1395681/1‑289 (MQ=255)
                                                                                                                                                                                                                                     tGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAtt                                              >  1:103134/1‑289 (MQ=255)
                                                                                                                                                                                                                                     tGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGAAGGAtt                                              >  1:203669/1‑289 (MQ=255)
                                                                                                                                                                                                                                       cgcAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt                                             >  1:1834815/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    tgttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCAAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCACCATTAAAACTCAGGATTTTCCTGGCGTAAcaa                               >  2:474172/10‑287 (MQ=255)
                                                                                                                                                                                                                                                      ttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGTTTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGTACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTACGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGCACATTAATGATGAGCAGGCCTAATCATTAACATCCAGTATTTTCCTTGCGTAACCaag                             >  2:1263548/8‑289 (MQ=255)
                                                                                                                                                                                                                                                      ttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAgg                                                                                                                                                     <  1:1621387/162‑1 (MQ=255)
                                                                                                                                                                                                                                                      ttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag                             >  2:1471839/8‑289 (MQ=255)
                                                                                                                                                                                                                                                      ttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag                             >  1:1740682/8‑289 (MQ=255)
                                                                                                                                                                                                                                                      ttggcgaggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag                             >  1:889324/8‑289 (MQ=255)
                                                                                                                                                                                                                                                            aggTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTg                       >  2:874995/2‑289 (MQ=255)
                                                                                                                                                                                                                                                              gTACGCAGGTTCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGaa                     >  1:1872159/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       ttCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGt             <  2:851968/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ttCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAACGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGt             >  1:930649/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                       ttCAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACAGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCATGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGtt            >  2:622101/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         cAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCa          >  2:37670/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         cAATGCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCa          >  2:232453/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             gCCTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTa      <  2:1322016/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cTTAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAAc    <  2:530189/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ttAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACa   >  2:1166694/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                 tAAACGCTGAAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAg  <  1:1427844/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                        
CTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTGCTGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGGCTGGTACGCAGGTTCAATGCCTTAAACGCACTAGGAC‑GATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACGCTACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCACTAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAAACTACTGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAG  >  CP042583/1977173‑1977734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A