Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F210 I1 R1	4928	368.7	6392010	97.5%	6232209	231.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	672,205	3 bp→TCG	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	672,205	0	A	T	100.0%	756.1 / NA	315	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (158/157); total (158/157)
*	CP042583	672,206	0	G	C	99.7%	741.1 / NA	315	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (158/156); total (158/157)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
*	CP042583	672,207	0	C	G	99.7%	742.1 / NA	315	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (158/155); minor base A (0/1); total (158/157)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 315 total aligned reads displayed.

CGCCGCCCACCAGGAGCTCATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGAGCCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGAGCATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCAGCCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGAGCGCGCGCAAAGCGAAAGCGGCAGGAAGCACGCGCTTCTGTATGGGCGCGGCGTGGAA  >  CP042583/671927‑672485

|||

             gaagacATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGAGAAACCGGCAGAGGGGAGGTCATGGATGGGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGa                                                                                                                                                                                                                                                                    <  2:3003216/283‑1 (MQ=255)

                 acATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAAc                                                                                                                                                                                                                                                               <  2:1130791/288‑1 (MQ=255)

                  cATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAAcc                                                                                                                                                                                                                                                              >  1:2737093/1‑289 (MQ=255)

                  cATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAAcc                                                                                                                                                                                                                                                              >  1:277473/1‑289 (MQ=255)

                            aCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGAt                                                                                                                                                                                                                                                    >  1:1564462/1‑289 (MQ=255)

                                           tCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGAt                                                                                                                                                                                                                                                    >  2:2696132/1‑274 (MQ=255)

                                           tCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGAt                                                                                                                                                                                                                                                    <  1:2696132/274‑1 (MQ=255)

                                            cAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcg                                                                                                                                                                                                                                     <  2:1448131/288‑1 (MQ=255)

                                                     tCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGc                                                                                                                                                                                                                           <  1:813644/289‑1 (MQ=255)

                                                         cTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCgcagca                                                                                                                                                                                                                                >  1:558582/1‑280 (MQ=255)

                                                         cTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATc                                                                                                                                                                                                                        >  1:1505744/1‑288 (MQ=255)

                                                                             ccaccGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTc                                                                                                                                                                                                   <  2:2599378/289‑1 (MQ=255)

                                                                                 cGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGt                                                                                                                                                                                               >  1:1190691/1‑289 (MQ=255)

                                                                                 cGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGt                                                                                                                                                                                               >  2:1283569/1‑289 (MQ=255)

                                                                                        aCCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTc                                                                                                                                                                                        >  2:59105/1‑289 (MQ=255)

                                                                                                     aGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTg                                                                                                                                                                            <  2:2111401/288‑1 (MQ=255)

                                                                                                          aTGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTgtcgatt                                                                                                                                                                      <  2:3143641/289‑7 (MQ=255)

                                                                                                                   tATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGa                                                                                                                                                             >  1:2880045/1‑289 (MQ=255)

                                                                                                                    tgggttgcaggATATGG‑GTTGGTTCAAAGCAAGATTGTTCGTTGTCATAATTGGAATGTAAAACAAAATGAAAAAATTTAAGTTTACAAATCTACACCCAATTAACAACAAAAAACACGTTTTGGGGAAGGCCCATGGCTCACCGCCCACGCTTGACAATTGTG‑CAAGGCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGc                                                                                                                                                           <  2:2812947/279‑1 (MQ=255)

                                                                                                                    aTGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAg                                                                                                                                                            <  1:1928867/289‑1 (MQ=255)

                                                                                                                        gTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAGAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTg                                                                                                                                                        >  1:3145714/1‑289 (MQ=255)

                                                                                                                         tGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTg                                                                                                                                                        >  1:947067/1‑288 (MQ=255)

                                                                                                                           ccAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAAcc                                                                                                                                                                                                                                                              <  1:515727/184‑1 (MQ=255)

                                                                                                                           ccAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAAcc                                                                                                                                                                                                                                                              >  2:515727/1‑184 (MQ=255)

                                                                                                                           ccAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTg                                                                                                                                                                                              <  2:687992/248‑1 (MQ=255)

                                                                                                                           ccAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCGGTCGATTAGGACCGGAGCTTGTcc                                                                                                                                                     >  2:1635653/1‑289 (MQ=255)

                                                                                                                                       ttGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTc                                                                                                                                                      >  1:1480709/1‑276 (MQ=255)

                                                                                                                                        tGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCAttt                                                                                                                                                                                                              >  1:2316949/1‑219 (MQ=255)

                                                                                                                                        tGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCaaa                                                                                                                                        >  2:2863801/1‑289 (MQ=255)

                                                                                                                                         ggTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGt                                                                                                                                                                                         >  2:1801071/1‑239 (MQ=255)

                                                                                                                                               aGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGc                                                                                                                                                                                                                                                         >  2:273738/1‑169 (MQ=255)

                                                                                                                                                    aGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAg                                                                                                                                                >  1:298708/1‑269 (MQ=255)

                                                                                                                                                        cGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAagct                                                                                                                        <  2:1643771/289‑2 (MQ=255)

                                                                                                                                                          tCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAagctc                                                                                                                       >  2:2577631/1‑286 (MQ=255)

                                                                                                                                                           ccGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAagctcg                                                                                                                      <  1:1639178/288‑4 (MQ=255)

                                                                                                                                                           ccGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAagctcg                                                                                                                      <  1:2076794/288‑4 (MQ=255)

                                                                                                                                                              ttGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCCGGTCAGCACGGTGCTGGCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCaaacgtccgcc                                                                                                                   >  2:2242456/1‑280 (MQ=255)

                                                                                                                                                               tGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTc                                                                                                                                                                                                   >  1:973060/1‑207 (MQ=255)

                                                                                                                                                                 tCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTg                                                                                                                                                                                              >  2:658640/1‑210 (MQ=255)

                                                                                                                                                                     aaTCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACAGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATTCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTCCCCGCAAagctcgtgcttcaaaa                                                                                                            >  1:679512/1‑275 (MQ=255)

                                                                                                                                                                      aTCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAAcc                                                                                                          <  2:1190691/289‑1 (MQ=255)

                                                                                                                                                                           cTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAg                                                                                                                                                                                                                   <  1:3128060/179‑1 (MQ=255)

                                                                                                                                                                           cTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTg                                                                                                                                                                                              <  1:1534156/200‑1 (MQ=255)

                                                                                                                                                                           cTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCgg                                                                                                        >  1:1167407/1‑286 (MQ=255)

                                                                                                                                                                                 aaCCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGc                                                                                                                                                                                                                           <  2:749702/165‑1 (MQ=255)

                                                                                                                                                                                    cAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGa                                                                                            <  1:252776/289‑1 (MQ=255)

                                                                                                                                                                                     aaaTTGAAAAGATTTAGGTTTACAAGTCTACACCTAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAg                                                                                           <  1:2217389/289‑1 (MQ=255)

                                                                                                                                                                                     aaaTTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAg                                                                                           <  2:2165427/289‑1 (MQ=255)

                                                                                                                                                                                     aaaTTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAg                                                                                           <  1:1776332/289‑1 (MQ=255)

                                                                                                                                                                                     aaaTTGAAAAAAGTTAGGTTTAAAAGTCTACACCAAATTCAGAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAg                                                                                           <  2:2183448/289‑1 (MQ=255)

                                                                                                                                                                                     aaaTGGAACAGAGTTAGGTTTACAAGTCTACACCGAAGTAACAACAAAAAACACGTTTTGGAGCAGCCCCATGGCTCACCGCCCACGCTGTAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAGACCGTTGCTGGATCTGCTGTTTGAATCGCAGCAGTTGCGTCGCGAGCATTTGGATCCTCGTCAGGTGCAGGTCAGCACGTTGCGGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCTCAAAGCTCGCGCTATAAAACCGGGCTGGAAGCCGAg                                                                                           <  2:1706771/289‑1 (MQ=255)

                                                                                                                                                                                         tGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAg                                                                                                                                                            >  1:216894/1‑220 (MQ=255)

                                                                                                                                                                                             aaGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGAt                                                                                   <  2:3129672/289‑1 (MQ=255)

                                                                                                                                                                                             aaGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGAt                                                                                   >  1:2817786/1‑289 (MQ=255)

                                                                                                                                                                                              aGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATg                                                                                  <  1:757170/289‑1 (MQ=255)

                                                                                                                                                                                                        ttACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAc                                                                        <  2:338337/289‑1 (MQ=255)

                                                                                                                                                                                                          aCAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGAt                                                                                                                                              >  2:1509665/1‑217 (MQ=255)

                                                                                                                                                                                                          aCAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAg                                                                      >  1:2006521/1‑289 (MQ=255)

                                                                                                                                                                                                          aCAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAg                                                                      >  1:1018665/1‑289 (MQ=255)

                                                                                                                                                                                                          aCAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCCCGGGCTACAAAACCGGGGCGGAAGCCGAGGGGCTGATGGaacgccctctt                                                                      >  2:77433/1‑280 (MQ=255)

                                                                                                                                                                                                                cTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGa                                                                                                                                                                                                           <  2:2324067/150‑1 (MQ=255)

                                                                                                                                                                                                                 tACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTgg                                                                                                                                                                                                                                                      <  1:470063/106‑1 (MQ=255)

                                                                                                                                                                                                                 tACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAg                                                                                                                                                <  1:2744162/208‑1 (MQ=255)

                                                                                                                                                                                                                   cacCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGgag                                                             >  1:1210795/1‑289 (MQ=255)

                                                                                                                                                                                                                   cacCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGgag                                                             >  1:2064318/1‑289 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTg                                                                                                                                                                                              >  2:207518/1‑155 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGc                                                                                                                                                                              >  2:2748031/1‑171 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGAc                                                                                                                                                                 >  2:3075562/1‑184 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAg                                                                                           >  2:2001612/1‑254 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGAt                                                                                   >  1:3061740/1‑262 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAc                                                                        <  2:1944162/273‑1 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAc                                                                        >  2:1421476/1‑273 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGgagtcggc                                                        >  1:2373431/1‑284 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGgagtcggc                                                        >  1:2942868/1‑284 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGAGGGAAGTTGAACAGGGTCTGgagtcggc                                                        >  2:1164479/1‑284 (MQ=255)

                                                                                                                                                                                                                        aaTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCAGAGCGGGTGCGGGAAGGTGAAAAGGTGCTGgagtgggg                                                        >  2:1037772/1‑284 (MQ=255)

                                                                                                                                                                                                                         aTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGtt                                                                                                                                                                                                                                            <  1:1674957/108‑1 (MQ=255)

                                                                                                                                                                                                                         aTTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAagctcg                                                                                                                      <  2:868087/226‑4 (MQ=255)

                                                                                                                                                                                                                                                     aGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGatcgacgc                           >  2:1548189/1‑282 (MQ=255)

                                                                                                                                                                                                                                                     aGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGatcgacg                            <  1:888683/288‑7 (MQ=255)

                                                                                                                                                                                                                                                     aGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGatcgacg                            <  2:2373431/288‑7 (MQ=255)

                                                                                                                                                                                                                                                        cccATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACgcgc                         <  1:1140053/288‑1 (MQ=255)

                                                                                                                                                                                                                                                        cccATGGCTCACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACgcgc                         <  1:2158986/288‑1 (MQ=255)

                                                                                                                                                                                                                                                        ccagtGGCCCACGCCCCACGCTGAGC‑ATTATCGCAAGTCACAGAATTATTGGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGGGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTTCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGGGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGatcgacg                            <  2:2520501/281‑7 (MQ=255)

                                                                                                                                                                                                                                                                 cACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAAcc                                                                                                          >  1:1970836/1‑198 (MQ=255)

                                                                                                                                                                                                                                                                 cACCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCAGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAg                                                                                                <  2:899469/208‑1 (MQ=255)

                                                                                                                                                                                                                                                                  aCCGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAAcc                                                                                                          >  1:2201391/1‑197 (MQ=255)

                                                                                                                                                                                                                                                                   ccGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAAc                                                                        >  1:860412/1‑230 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATctgct                                                                                                                                                                                                                                               >  1:70486/1‑62 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGatcgacg                            <  1:1351786/273‑7 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGatcgacg                            >  2:1351786/1‑267 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGat                                 <  2:2428428/268‑2 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGat                                 >  1:2428428/1‑267 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCCACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGgcg            >  1:1409873/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                       ccACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACgcgc                         >  1:1838017/1‑273 (MQ=255)

                                                                                                                                                                                                                                                                       ccACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACgcgc                         <  2:1838017/273‑1 (MQ=255)

                                                                                                                                                                                                                                                                       ccACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATGGGcgcg          <  1:642471/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                        cACGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTGGGCGCGCAAAGCGAAAGGGGCAGGATCGACGCGCTTCTGTATGGgcggtgc        >  2:293611/1‑285 (MQ=255)

                                                                                                                                                                                                                                                                          cGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGGGATTCGGTAGGGGCGCGGCGt      >  2:47253/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                          cGCTGGAC‑ATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGTCGATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCTCGCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGTCGGCGCGCAAAGCGAAAGCGGCAGGATCGACGCGCTTCTGTATggg              <  1:2608663/281‑1 (MQ=255)

|||

CGCCGCCCACCAGGAGCTCATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGAC‑ATTGAGCCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCGTCAGGTGCAGGTCAGCACGTTGCTGAGCATTAAGACCGGAGCTTGTCCGGAAGATTGCAAATACTGCCCGCAAAGCAGCCGCTACAAAACCGGGCTGGAAGCCGAGCGGTTGATGGAAGTTGAACAGGTGCTGGAGAGCGCGCGCAAAGCGAAAGCGGCAGGAAGCACGCGCTTCTGTATGGGCGCGGCGTGGAA  >  CP042583/671927‑672485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A