Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F210 I1 R1	4928	368.7	6392010	97.5%	6232209	231.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	750,033	3 bp→CGA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	750,033	0	G	C	100.0%	831.9 / NA	349	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (169/180); total (169/180)
*	CP042583	750,034	0	C	G	100.0%	830.2 / NA	349	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (169/180); total (169/180)
*	CP042583	750,035	0	T	A	99.7%	830.8 / NA	349	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (169/178); minor base G (0/1); total (169/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

CCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGGCTTGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCGCTGCAGAGAATA‑CCGCTCATGCTACCGATGCTCAGACCAAAGAGAACACCGCCCATTTCAGCGATGCTGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGACTAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGA  >  CP042583/749756‑750307

|||

    cccGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑cc                                                                                                                                                                                                                                                                      >  1:16898/1‑288 (MQ=255)

        acacCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCa                                                                                                                                                                                                                                                                 >  1:649224/1‑289 (MQ=255)

                    gCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAtcg                                                                                                                                                                                                                                                     >  1:1496101/1‑287 (MQ=255)

                      cAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTTCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAtcgac                                                                                                                                                                                                                                                   <  2:327612/289‑5 (MQ=255)

                          gTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAtcgacaga                                                                                                                                                                                                                                                <  1:1788370/288‑8 (MQ=255)

                                 ggCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAtcg                                                                                                                                                                                                                                                     <  2:1160941/276‑3 (MQ=255)

                                  gCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAgaga                                                                                                                                                                                                                                       >  2:1684272/1‑289 (MQ=255)

                                  gCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAgaga                                                                                                                                                                                                                                       >  1:1158220/1‑289 (MQ=255)

                                             cGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAACAGAACACCGCCCAt                                                                                                                                                                                                                            >  2:2724666/1‑289 (MQ=255)

                                                gaatcatcGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGGCGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCAttt                                                                                                                                                                                                                          <  2:551804/286‑1 (MQ=255)

                                                gCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAaca                                                                                                                                                                                                                                    <  2:1553841/278‑1 (MQ=255)

                                                gCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAaca                                                                                                                                                                                                                                    >  1:1553841/1‑278 (MQ=255)

                                                            aaaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGAtcgagac                                                                                                                                                                                                             <  2:649224/289‑7 (MQ=255)

                                                                  tCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACagagag                                                                                                                                                                                                       >  1:942132/1‑289 (MQ=255)

                                                                   cTTAATCATTTCCCGCTCAACGGCAGCCCCTTCAACGTTTATAGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAgagaga                                                                                                                                                                                                      <  2:39554/289‑1 (MQ=255)

                                                                           tttCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATcgcg                                                                                                                                                                                              <  2:1612920/289‑1 (MQ=255)

                                                                           tttCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATcgcg                                                                                                                                                                                              <  1:1401181/289‑1 (MQ=255)

                                                                            ttCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAtcga                                                                                                                                                                                                                                                    <  1:1429342/234‑4 (MQ=255)

                                                                            ttCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGCGACAGAGAGAATAACCCgg                                                                                                                                                                                             >  2:1734988/1‑289 (MQ=255)

                                                                                 gCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAACGACAAACCAAAGAGAACACAGCCCATTTCAGCGATCGAGACAGAAAGAAACACGCGGATAGc                                                                                                                                                                                        >  2:2548920/1‑289 (MQ=255)

                                                                                      aCGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGAtcgacaga                                                                                                                                                                                                                                                >  2:1471901/1‑221 (MQ=255)

                                                                                              aCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACggg                                                                                                                                                                             <  2:2160210/287‑1 (MQ=255)

                                                                                               ccTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGtt                                                                                                                                                                           >  1:58929/1‑288 (MQ=255)

                                                                                                  tCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACggg                                                                                                                                                                             <  2:1243226/283‑1 (MQ=255)

                                                                                                      cGTTTAGCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGa                                                                                                                                                                   <  1:3191583/289‑1 (MQ=255)

                                                                                                          tATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACg                                                                                                                                                                 >  1:2863929/1‑287 (MQ=255)

                                                                                                                cACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                                                                          <  1:867099/288‑1 (MQ=255)

                                                                                                                     ccGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGGTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                                                                    >  1:1566152/1‑289 (MQ=255)

                                                                                                                             ccAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATcg                                                                                                                                                                                                >  2:1107551/1‑237 (MQ=255)

                                                                                                                                aaaCTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                                                                         <  2:1311823/289‑1 (MQ=255)

                                                                                                                                     tGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGCTGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCAATTTCAGCGATCGAGACAGAGAGAATACCGCGGATAGCAGGCGCACGGGTCGTCCAGGACGCCATGAACAGGCCTGGCAAAAGgaagaaa                                                                                                                                    >  2:85913/1‑288 (MQ=255)

                                                                                                                                     tGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCCGGCCAAAAGAAGAAc                                                                                                                                    >  1:247179/1‑289 (MQ=255)

                                                                                                                                                    gagaCCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGt                                                                                                                                                                                  <  1:2831411/228‑1 (MQ=255)

                                                                                                                                                       aCCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGGTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGtt                                                                                                                  <  2:1566152/289‑1 (MQ=255)

                                                                                                                                                        ccAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGtt                                                                                                                  <  2:2288448/288‑1 (MQ=255)

                                                                                                                                                                aaaGAGCATGGGAGACGTCAGCCAGAGTGCGAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGGTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTc                                                                                                         <  2:2448105/289‑1 (MQ=255)

                                                                                                                                                                aaaGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTc                                                                                                         <  2:2969520/289‑1 (MQ=255)

                                                                                                                                                                         gggcgatGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAGAGAAGAACATAAACAGCGCCCAGGTACGGCGTTTCAAGGCAtt                                                                                                 >  1:1916408/7‑288 (MQ=255)

                                                                                                                                                                         gggcgatGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAtt                                                                                                 >  1:762152/7‑288 (MQ=255)

                                                                                                                                                                              atGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGATGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACgtga                                                                                           >  2:2917287/2‑286 (MQ=255)

                                                                                                                                                                                      cAGAGTGCCATACTTAATATACTCAGCCCAATACAAGCGCCAGACATCGTGACCAAGATGACA‑TTACGTGTCCCAAACCGTTTCACTCACCACGCCGAGCAGCGAATA‑CCGCTGATCGCACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  2:1916408/289‑1 (MQ=255)

                                                                                                                                                                                      cAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  1:2262646/289‑1 (MQ=255)

                                                                                                                                                                                      cAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  2:1974371/289‑1 (MQ=255)

                                                                                                                                                                                      cAGAGGACCAGACTTAATATCATCAGCCCGAACAATGCGCAGGACATCGTGACCAGGAGTACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  2:3115793/289‑1 (MQ=255)

                                                                                                                                                                                         agTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  1:1631306/1‑286 (MQ=255)

                                                                                                                                                                                           tGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCCGCTCATCGAACCGATCAACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATaaa                                                                                                                               <  1:3053933/241‑1 (MQ=255)

                                                                                                                                                                                           tGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAaca                                                                                                                                                                                                                                    >  2:2584174/1‑139 (MQ=255)

                                                                                                                                                                                            gCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCa                                                                             <  1:1734988/289‑1 (MQ=255)

                                                                                                                                                                                             ccAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAgagaga                                                                                                                                                                                                      <  2:956864/167‑1 (MQ=255)

                                                                                                                                                                                                     aaTATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACaga                                                                                                                                                                                                          >  2:1012742/1‑155 (MQ=255)

                                                                                                                                                                                                     aaTATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACaga                                                                                                                                                                                                          <  1:1012742/155‑1 (MQ=255)

                                                                                                                                                                                                     aaTATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACg                                                                    >  1:2746843/1‑289 (MQ=255)

                                                                                                                                                                                                           atcatcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGAtcgag                                                                                                                                                                                                               >  2:800556/1‑140 (MQ=255)

                                                                                                                                                                                                           atcatcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  1:1895094/1‑268 (MQ=255)

                                                                                                                                                                                                           atcatcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  2:2865844/268‑1 (MQ=255)

                                                                                                                                                                                                            tcatcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                                                                       <  2:467429/215‑1 (MQ=255)

                                                                                                                                                                                                             catcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAgg                                                                                                                                                                    <  1:1817149/185‑1 (MQ=255)

                                                                                                                                                                                                             catcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  1:2281242/1‑266 (MQ=255)

                                                                                                                                                                                                             catcCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGACAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTc                                                                  <  2:3142379/283‑1 (MQ=255)

                                                                                                                                                                                                                    aTCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  2:1813612/1‑259 (MQ=255)

                                                                                                                                                                                                                         tGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGc                                                                                                                                                                                        >  1:1179093/1‑153 (MQ=255)

                                                                                                                                                                                                                         tGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGc                                                                                                                                                                                        <  2:1179093/153‑1 (MQ=255)

                                                                                                                                                                                                                         tGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  2:114689/254‑1 (MQ=255)

                                                                                                                                                                                                                          gcgcAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGATGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGa                                               <  1:2917287/289‑1 (MQ=255)

                                                                                                                                                                                                                           cgcAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCTCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCAc                                                                     <  1:2011420/266‑1 (MQ=255)

                                                                                                                                                                                                                           cgcAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTc                                                                  >  2:2236411/1‑269 (MQ=255)

                                                                                                                                                                                                                            tcAGGACATCGTGACCAGGATGACA‑GTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCGCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGac                                              <  2:739644/287‑1 (MQ=255)

                                                                                                                                                                                                                              aGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  1:2713239/249‑1 (MQ=255)

                                                                                                                                                                                                                                gACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                                                                        >  1:2368767/1‑258 (MQ=255)

                                                                                                                                                                                                                                gACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGAt                                                                        <  2:2368767/258‑1 (MQ=255)

                                                                                                                                                                                                                                   aTCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCATAATAAGAAGAGGGAAGACAACATTa                                       >  1:398938/1‑288 (MQ=255)

                                                                                                                                                                                                                                     cGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATcg                                                                                                                                                                                                >  2:572834/1‑133 (MQ=255)

                                                                                                                                                                                                                                     cGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACgtga                                                                                           <  1:46641/234‑4 (MQ=255)

                                                                                                                                                                                                                                     cGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCa                                    <  2:2837698/289‑1 (MQ=255)

                                                                                                                                                                                                                                     cGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCa                                    <  1:3102429/289‑1 (MQ=255)

                                                                                                                                                                                                                                     cGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCa                                    <  2:873969/289‑1 (MQ=255)

                                                                                                                                                                                                                                         caaaGGATGACAGTAACGT‑TCCAAAAGCGTGACCCTAACCACGCCGCGCAGAGAATA‑CCGCGAATCGAACCGATCGACAGACCAAAGGGAACACCGCCAAGTGCAGCGATCGATACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCTAGGACGCCAGTAACAGGCCTGGGAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTGACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAgg                                 <  2:3055790/285‑1 (MQ=255)

                                                                                                                                                                                                                                              gATGCCA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCaa                                   <  2:1410911/281‑1 (MQ=255)

                                                                                                                                                                                                                                               aTGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTtgtg                            <  2:1869888/287‑1 (MQ=255)

                                                                                                                                                                                                                                                 gACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGcc                                                                                                                                                               <  1:2561429/154‑1 (MQ=255)

                                                                                                                                                                                                                                                        aCGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTa                  <  2:2746843/289‑1 (MQ=255)

                                                                                                                                                                                                                                                         ctggtCCCAACGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGTTCATAGATCACGTCAAAATAAGAA‑AGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTaca                <  1:1220773/286‑1 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTTCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTac                 >  1:2957433/1‑289 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGATAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAGGGTTGTGTACATTTGTac                 >  1:1131855/1‑289 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                                                                       <  2:2066058/171‑1 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                                                                       >  1:2066058/1‑171 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAAc                                                                                                                              >  1:1025983/1‑180 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  1:545477/1‑223 (MQ=255)

                                                                                                                                                                                                                                                         cGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTac                 >  1:980111/1‑289 (MQ=255)

                                                                                                                                                                                                                                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                                                                         <  1:2152596/165‑1 (MQ=255)

                                                                                                                                                                                                                                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  2:2321465/219‑1 (MQ=255)

                                                                                                                                                                                                                                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   <  1:1324114/219‑1 (MQ=255)

                                                                                                                                                                                                                                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  1:2321465/1‑219 (MQ=255)

                                                                                                                                                                                                                                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                                                                                   >  2:1035427/1‑219 (MQ=255)

                                                                                                                                                                                                                                                             tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCa                                    >  1:3139753/1‑266 (MQ=255)

                                                                                                                                                                                                                                                                    gCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCa                                    <  1:1847994/259‑1 (MQ=255)

                                                                                                                                                                                                                                                                    gCGTTTCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCa                                    >  2:1847994/1‑259 (MQ=255)

                                                                                                                                                                                                                                                                       tttCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagag   >  2:211880/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                        ttCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                                                                         >  1:1047125/1‑154 (MQ=255)

                                                                                                                                                                                                                                                                        ttCACTAACCACGCCGAGCAGAGAATA‑CCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCagagag   <  1:211880/288‑1 (MQ=255)

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CCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGGCTTGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACA‑TTACGTGTCCCAAAGCGTTTCACTAACCACGCGCTGCAGAGAATA‑CCGCTCATGCTACCGATGCTCAGACCAAAGAGAACACCGCCCATTTCAGCGATGCTGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGACTAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGAGGGAAGACAACATTAGCAAGGTTGTGTACATTTGTACACAATTGCAGAGAGA  >  CP042583/749756‑750307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A