Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F210 I1 R1	4928	368.7	6392010	97.5%	6232209	231.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	1,818,188	3 bp→TGA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	1,818,188	0	A	T	99.7%	865.7 / ‑4.2	360	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (194/165); minor base . (0/1); total (194/166)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
*	CP042583	1,818,189	0	C	G	99.4%	851.1 / NA	360	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (193/165); minor base T (1/1); total (194/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,818,190	0	T	A	99.7%	859.1 / NA	360	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (192/165); total (194/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

CTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑GCTGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTACTCAGACCGATCACGCTATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATAT  >  CP042583/1817916‑1818468

|||

         aaTCAGTTGTGATGCCGCGCAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGAATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCATTTGTAGCGCAATAAGAATTTGCTTCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCg                                                                                                                                                                                                                                                                  <  1:550966/289‑1 (MQ=255)

                                                  cgTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGCCA‑ACCGAACACCGAAACTTTCGGGATATAAGCCACCGTTGCTTTTCCCACGATGGTTAACaaat                                                                                                                                                                                                                        >  1:2474714/1‑288 (MQ=255)

                                                          gACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTc                                                                                                                                                                                                                >  1:3050449/1‑289 (MQ=255)

                                                            cTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTcac                                                                                                                                                                                                              <  2:1372677/289‑1 (MQ=255)

                                                                 tttGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGt                                                                                                                                                                                                          >  1:416070/1‑288 (MQ=255)

                                                                      acaatcacacaAGAGATGTGTGTGGCGTAGCACGTGTGGCAGCGCTGAGGCCCCGCGCGTTCAAGCAGTAATGCACGGCTGCGTT‑CGAGACGACCACCTTATTTGGACCCAGCAGCGTGTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCAACTGCGGACGCTGGGCAAAGAACTGCACAATGAGGTTAATTTTTTACAGACAGATAATCGAATCTAGCGGGATATAGGCCACCGTCGCTTGGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    <  2:2517368/280‑1 (MQ=255)

                                                                      aCAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    <  2:2091310/289‑1 (MQ=255)

                                                                          tCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCaga                                                                                                                                                                                                >  1:3147610/1‑289 (MQ=255)

                                                                          tCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCACGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGTGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCaga                                                                                                                                                                                                >  1:3179843/1‑289 (MQ=255)

                                                                           ccaccaAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCagag                                                                                                                                                                                               <  1:1633641/289‑1 (MQ=255)

                                                                               caAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTACTTTTTGACAGACCGATCACCGAATATTTCGGGATATAGGCCACCGTCGCTTCGCCATCGATGG‑TAACAAAATg                                                                                                                                                                                                                      >  1:1744855/1‑262 (MQ=255)

                                                                               caAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGat                                                                                                                                                                                           >  2:396528/1‑289 (MQ=255)

                                                                               caAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGat                                                                                                                                                                                           <  1:652712/289‑1 (MQ=255)

                                                                                  gagagGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCg                                                                                                                                                                                                                                                                  <  1:2079309/216‑1 (MQ=255)

                                                                                      ggTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATgg                                                                                                                                                                                                                                 <  2:2164852/245‑1 (MQ=255)

                                                                                      ggTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATgg                                                                                                                                                                                                                                 >  1:2164852/1‑245 (MQ=255)

                                                                                       gTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGAt                                                                                                                                                                                                                                   >  1:376800/1‑242 (MQ=255)

                                                                                       gTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATg                                                                                                                                                                                                                      >  1:1413751/1‑254 (MQ=255)

                                                                                       gTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATg                                                                                                                                                                                                                      <  2:1413751/254‑1 (MQ=255)

                                                                                       gTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATAGAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTTCTTGGCAGAGTGATATCccgc                                                                                                                                                                                    >  2:2946979/1‑288 (MQ=255)

                                                                                        tCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGAGGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGGGAGATcgcggcc                                                                                                                                                                                  >  2:498901/1‑286 (MQ=255)

                                                                                            tGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGt                                                                                                                                                                               <  1:219790/288‑1 (MQ=255)

                                                                                             gTCGTGTCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGCTCACAGGTGCTGGTTAGAGTGATATCGCGCACGGTGa                                                                                                                                                                             <  1:1846490/289‑1 (MQ=255)

                                                                                                        ggTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTcac                                                                                                                                                                                                              >  2:3013491/1‑245 (MQ=255)

                                                                                                         gTTGCATCGCGGATGCCACGCTCCCTCACGCAGTAATGCACCGCGGCGAT‑CGAGACAGCCATGTTATGGGTGCGCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCGGACGTTCCTGCACCTACGGATGCAGGGCAAAGAACTGCACAACGCGGTTAATTTTTGACAGACCGACCACCGTATCGTTCGGGATCTAGGCCACCGTCGCGTCGCCATCGATGG‑TAACAAAATGGTGTTCACAGTTGGTGCTCAAATTGCTATCGCCCACGGTGCCCATTTCAttg                                                                                                                                                                  >  2:315668/1‑286 (MQ=255)

                                                                                                              aTCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCttt                                                                                                                                                                                                                                                                    >  2:1784244/1‑186 (MQ=255)

                                                                                                              aTCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTc                                                                                                                                                            >  2:454918/1‑289 (MQ=255)

                                                                                                              aTCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCCATGG‑TAACAAAATGGTGTTCACAGGTGCTGGGCAGAGTGCAATCGCGCACGGTGACCATTTCATCGACCTTc                                                                                                                                                            >  2:1490666/1‑289 (MQ=255)

                                                                                                                  cgGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGTTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCAttt                                                                                                                                                        >  1:1374480/1‑289 (MQ=255)

                                                                                                                                 ttCACGCAGTAATGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACGATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACg                                                                                                                                                                                 <  1:2236932/249‑1 (MQ=255)

                                                                                                                                           aaTGCACCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCgg                                                                                                                               <  1:1463953/289‑1 (MQ=255)

                                                                                                                                            aTGCACCGCGTCGATCGGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    <  1:1758244/220‑1 (MQ=255)

                                                                                                                                                aacgcgTCGAT‑CGAGACAGCCACGTTATTGGTGCCAAGCAGCGGTGGTAGCGAAAGAAGAATTTGCGGCGTCAGACGATCCTGCACCGGCGGACGCTGTGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAAt                                                                                                                          <  2:3186294/287‑1 (MQ=255)

                                                                                                                                                aCCGCGTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGaaa                                                                                                                           <  2:3050449/288‑1 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCg                                                                                                                                                                                                                                                                  <  1:573569/150‑1 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATgg                                                                                                                                                                                                                                 >  1:108738/1‑183 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATgg                                                                                                                                                                                                                                 >  1:3008592/1‑183 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTcac                                                                                                                                                                                                              <  2:2651814/201‑1 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGt                                                                                                                                                                                                          >  1:2605218/1‑205 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    <  2:73680/211‑1 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGc                                                                                                                      >  2:2233180/1‑289 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGc                                                                                                                      >  1:2812001/1‑289 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGc                                                                                                                      >  1:1907231/1‑289 (MQ=255)

                                                                                                                                                    cgTCGAT‑CGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGc                                                                                                                      >  1:855896/1‑289 (MQ=255)

                                                                                                                                                      gtcgatcgagACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGc                                                                                                                      <  1:2946979/279‑1 (MQ=255)

                                                                                                                                                      gtcgatcgagACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCGTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCg                                                                                                                     <  1:1226388/280‑1 (MQ=255)

                                                                                                                                                       tcgatcgagACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCg                                                                                                                     <  2:15330/280‑1 (MQ=255)

                                                                                                                                                                         ttATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCAtt                                                                                                                                                         >  1:1169853/1‑234 (MQ=255)

                                                                                                                                                                         ttATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCAtt                                                                                                                                                         <  2:1169853/234‑1 (MQ=255)

                                                                                                                                                                            ttGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTcac                                                                                                                                                                                                              <  1:1538062/178‑1 (MQ=255)

                                                                                                                                                                              ggTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGc                                                                                                                      <  2:1269303/264‑1 (MQ=255)

                                                                                                                                                                              ggTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCa                                                                                             <  2:2874187/289‑1 (MQ=255)

                                                                                                                                                                              ggTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCa                                                                                             <  1:102583/289‑1 (MQ=255)

                                                                                                                                                                                  cccAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGa                                                                                                                                                                                                                                                                                 <  2:573903/106‑1 (MQ=255)

                                                                                                                                                                                  cccAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAAt                                                                                                                          >  1:264480/1‑256 (MQ=255)

                                                                                                                                                                                  cccAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCg                                                                                                                     >  1:1511540/1‑261 (MQ=255)

                                                                                                                                                                                  cccAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGAACACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCg                                                                                          >  1:439614/1‑288 (MQ=255)

                                                                                                                                                                                   ccAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGac                                                                                        >  2:2138507/1‑289 (MQ=255)

                                                                                                                                                                                    cagcagCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGaca                                                                                       >  1:952568/1‑289 (MQ=255)

                                                                                                                                                                                      gcagcGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    >  1:46654/1‑178 (MQ=255)

                                                                                                                                                                                      gcagcGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGacat                                                                                      >  1:61994/1‑288 (MQ=255)

                                                                                                                                                                                       cagcGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGacatac                                                                                    >  1:2915657/1‑289 (MQ=255)

                                                                                                                                                                                                    gcAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    >  2:2695993/1‑164 (MQ=255)

                                                                                                                                                                                                    gcAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAgcgatgc                                                                       >  1:3066312/1‑289 (MQ=255)

                                                                                                                                                                                                       aTAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGAc                                                                                                                                                                <  2:657432/197‑1 (MQ=255)

                                                                                                                                                                                                          aGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGatgcgatgcg                                                                 <  2:192969/289‑1 (MQ=255)

                                                                                                                                                                                                                  ctgcGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTcc                                                         >  2:552870/1‑289 (MQ=255)

                                                                                                                                                                                                                      gTCCGACGTTCCTACACCTGCGACCGCTGGGCAAAGAACTGCACAATGCGGTTAATGTTTGACAGACCGATAACCGAATCTTTCGGGATCTAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATc                                                      <  2:1956619/288‑1 (MQ=255)

                                                                                                                                                                                                                               tCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGgag                                                                                                      <  2:1580535/231‑1 (MQ=255)

                                                                                                                                                                                                                                ccTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCgatgcgatgc                                                                  <  1:2399671/266‑1 (MQ=255)

                                                                                                                                                                                                                                ccTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCgatgcgatgc                                                                  >  2:2399671/1‑266 (MQ=255)

                                                                                                                                                                                                                                ccTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATACGATGCGGCGTTTCCATCAAACTGtcatc                                           >  2:800839/1‑289 (MQ=255)

                                                                                                                                                                                                                                         gCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGc                                        >  1:775732/1‑283 (MQ=255)

                                                                                                                                                                                                                                         gCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGt                                   >  2:2440298/1‑288 (MQ=255)

                                                                                                                                                                                                                                         gCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTc                                  >  1:2846387/1‑289 (MQ=255)

                                                                                                                                                                                                                                          cGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATgg                                                                                                                                                                                                                                 <  1:940897/98‑1 (MQ=255)

                                                                                                                                                                                                                                          cGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGt                                   >  2:2037327/1‑287 (MQ=255)

                                                                                                                                                                                                                                          cGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGt                                   <  1:2037327/287‑1 (MQ=255)

                                                                                                                                                                                                                                                         gAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTcagcag                      <  1:159307/285‑1 (MQ=255)

                                                                                                                                                                                                                                                             tGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGt                                                                                                                                                                                                    <  2:1135275/107‑1 (MQ=255)

                                                                                                                                                                                                                                                                 caATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTc          <  2:865123/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                  aaTGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCg         <  2:104800/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCa                                                                                                                                                >  2:1749815/1‑152 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAAt                                                                                                                          <  2:2591986/174‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCa                                                                                             <  2:2743229/203‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAg                                                                             <  1:2675840/219‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGtca                                             >  1:698828/1‑251 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGtca                                             <  2:698828/251‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTcag                         >  1:257444/1‑271 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTaagc                        >  2:2813120/1‑272 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCgg        >  1:1320108/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCgg        >  1:2520692/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCgg        >  1:2427096/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCgg        >  2:2564414/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCA‑GGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCa                                                                                             <  2:941746/202‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTAGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGATGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTAAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGATAAAATTTCATCGACATACATCTTAGCGATGCGATGCGGCGTTTCCATCAAACTTGCATCAGCCAGGTCGAGATTCAGCTGCTGCATGATTTCgg        >  2:2628338/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                        gTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCat    <  1:2721929/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                         ttAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGaca                                                                                       <  1:2333348/204‑1 (MQ=255)

                                                                                                                                                                                                                                                                         ttAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGaca                                                                                       >  2:2333348/1‑204 (MQ=255)

                                                                                                                                                                                                                                                                         ttAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCataa  >  1:1938601/1‑288 (MQ=255)

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CTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGAT‑GCTGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTACTCAGACCGATCACGCTATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGG‑TAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATAT  >  CP042583/1817916‑1818468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A