Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 588,252 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583588,2520AT99.5% 465.0 / NA 200intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (83/115);  minor base G (1/0);  total (85/115)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583588,2530GC99.0% 470.6 / NA 200intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (85/113);  minor base A (0/2);  total (85/115)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583588,2540CG99.5% 468.0 / NA 200intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (85/114);  minor base A (0/1);  total (85/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

CAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGAGTGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGAGTGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCAGTTTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCAGCATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGAGTGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGGCTAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCGCTTAATTTTATTACGTCTTCTCG  >  CP042583/587972‑588531
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
cAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGatgatg                                                                                                                                                                                                                                                                                 <  1:1665884/289‑1 (MQ=255)
  gagGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCa                                                                                                                                                                                                                                                                               >  1:1136268/1‑289 (MQ=255)
        tAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCgg                                                                                                                                                                                                                                                                         >  1:1792943/1‑289 (MQ=255)
        tAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCgg                                                                                                                                                                                                                                                                         <  1:835503/289‑1 (MQ=255)
               tAGCATTGCGGTAGATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattatt                                                                                                                                                                                                                                                                  >  2:291288/1‑289 (MQ=255)
               tAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGACAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattatt                                                                                                                                                                                                                                                                  >  2:1597896/1‑289 (MQ=255)
                aGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattatt                                                                                                                                                                                                                                                                  <  1:459029/288‑1 (MQ=255)
                            aaTATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGt                                                                                                                                                                                                                                                                            <  2:32657/266‑1 (MQ=255)
                                 tGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGt                                                                                                                                                                                                                                                                            >  2:1344715/1‑261 (MQ=255)
                                 tGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCag                                                                                                                                                                                                                                                >  1:189367/1‑289 (MQ=255)
                                         tCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCGCGATGATGCATGTCGGGCTTATTGAGTTACCACGCTGCCagtcagaaaa                                                                                                                                                                                                                                        >  2:1686484/1‑281 (MQ=255)
                                                  aGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCa                                                                                                                                                                                                                               >  1:1988315/1‑289 (MQ=255)
                                                        aTTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTg                                                                                                                                                                                                                                     <  2:1197456/277‑1 (MQ=255)
                                                        aTTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTg                                                                                                                                                                                                                                     >  1:1197456/1‑277 (MQ=255)
                                                        aTTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                                                                                                                                                                                                                         >  1:327941/1‑289 (MQ=255)
                                                            acggtgggATAACTTACCTGATAATACCCGCCGGTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                                                                                                                                                                                                                      <  2:977959/281‑1 (MQ=255)
                                                             cGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                                                                                                                                                                                                                     <  1:1686484/288‑1 (MQ=255)
                                                                ggtgATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                                                                                                                                                                                  <  1:977487/288‑1 (MQ=255)
                                                                 gtgATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGAt                                                                                                                                                                                                                >  1:981119/1‑289 (MQ=255)
                                                                     tAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGA‑TTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGATTTGAAAGCGATAAATa                                                                                                                                                                                                           <  1:669838/289‑1 (MQ=255)
                                                                      acctatcttgtagataCCGGACGCTGGCGGTCGGCAGTCGGGAACTAAAAAGACATTTCTTAGAGTCATAATAGGTCCGGAAATGAAAAGGTCGGTTTAAGGCCCGGTTGACAATGCAACACCTCATCTTTTGTGCGGTTTTGACATGGAAAGCGCGGCTCTCGACCGGTAGTCAATGAGGATTATGTAAGCGAAAGCGGAGTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGGCAGAAGATTGCCAGCAATAATTGAAAGCgatagata                                                                                                                                                                                                           <  2:223960/276‑1 (MQ=255)
                                                                      aactaaccgactaatcCCCGCCGCCGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATAGGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTGTGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTGTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                                                                                                                                                                           <  2:465904/278‑1 (MQ=255)
                                                                      aaCTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                                                                                                                                                                           <  1:601643/289‑1 (MQ=255)
                                                                      aaCTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                                                                                                                                                                           >  1:560687/1‑289 (MQ=255)
                                                                      aaCTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                                                                                                                                                                           <  2:395306/289‑1 (MQ=255)
                                                                      aaCTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                                                                                                                                                                           <  1:1199765/289‑1 (MQ=255)
                                                                      aaCTTACCGAATAATACCCGCCGCTGGAAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                                                                                                                                                                           <  2:975292/289‑1 (MQ=255)
                                                                          tACCTGAAAATACCCGCAGTTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATAcaca                                                                                                                                                                                                       <  1:1479560/289‑1 (MQ=255)
                                                                           aCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGc                                                                                                                                                                                                                                                   <  2:542632/244‑1 (MQ=255)
                                                                           aCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGAGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATGATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAAACTTGAAAACAGTAAATACACAt                                                                                                                                                                                                      >  2:1090946/1‑289 (MQ=255)
                                                                           aCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGCCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGAATTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGACAGAAATTTGCGCGCCAGAAATGCAAGCACTAAATagacct                                                                                                                                                                                                      >  2:572277/1‑287 (MQ=255)
                                                                            ccTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAtt                                                                                                                                                                                                     >  2:355971/1‑289 (MQ=255)
                                                                              tGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGat                                                                                                                                                                                                                                                                      >  1:157012/1‑222 (MQ=255)
                                                                                ataataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGCGTTACGACGCTGCCAGTCAGAAAATTGCCAGAAAGAATTGAACGCGATAAATACAAAATaaaa                                                                                                                                                                                                 >  2:1510291/1‑289 (MQ=255)
                                                                                ataataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                                                                                                                                                                                                                     >  1:1001173/1‑269 (MQ=255)
                                                                                ataataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATCAATACACATTaaaa                                                                                                                                                                                                 >  1:698721/1‑289 (MQ=255)
                                                                                    taatcgccgcTGGAAGTTGGCAGGCGGGAACGAAAAAGAACTTACTTATCTGCATAATATGTAAGCACAAGTAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACAACTCATGTTTTATGCGGGTTTGCGAGGGCAAGCCCGGCACTAGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGATAGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGt                                                                                                                                                                                             <  1:1090946/285‑1 (MQ=255)
                                                                                    taCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGt                                                                                                                                                                                             <  2:203964/289‑1 (MQ=255)
                                                                                       ccgccgCTGGCAGTTGTCAGGCGGGAACGACAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGAAGCCGCCAGTCCGAAAATTGCCAGCACGAAGTGAAAGGTAAAAATACACATTAAAATAGTg                                                                                                                                                                                            >  2:1756725/1‑287 (MQ=255)
                                                                                         gccgcTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGt                                                                                                                                                                                         >  2:1276604/1‑288 (MQ=255)
                                                                                          ccgcTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattat                                                                                                                                                                                                                                                                   >  1:468614/1‑213 (MQ=255)
                                                                                          ccgcTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattat                                                                                                                                                                                                                                                                   <  2:468614/213‑1 (MQ=255)
                                                                                          ccgcTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                                                                                                                                                                                       >  1:303070/1‑289 (MQ=255)
                                                                                          ccgcTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTATTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                                                                                                                                                                                       >  1:1544324/1‑289 (MQ=255)
                                                                                             cTGGCAGTTGTCAGGCGGGAACGAAAAGGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATAcaca                                                                                                                                                                                                       <  1:998785/270‑1 (MQ=255)
                                                                                              tGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGa                                                                                                                                                                                   >  2:784423/1‑289 (MQ=255)
                                                                                                      tgcaggCGGGAAAGAAAAAGAACTTACTTAGCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                                                                                                                                                                           <  2:189367/285‑1 (MQ=255)
                                                                                                      gtcaggCTGGAACAAAAAAAAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAATTCCCGGTTGGCAATGCTATACCTGATTTTTTATGCGGGTTTGCCATGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                                                                                                                                                                           <  1:366797/285‑1 (MQ=255)
                                                                                                            cGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc                                                                                                                                                                      <  1:1452127/288‑1 (MQ=255)
                                                                                                            cGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc                                                                                                                                                                      <  1:284202/288‑1 (MQ=255)
                                                                                                               gAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGa                                                                                                                                                                  >  2:1073838/1‑289 (MQ=255)
                                                                                                                 aCGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAAc                                                                                                                                                                <  1:571965/289‑1 (MQ=255)
                                                                                                                           acttacttATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTa                                                                                                                                                        <  1:1276604/287‑1 (MQ=255)
                                                                                                                           acttacttATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTAt                                                                                                                                                       <  1:1073838/288‑1 (MQ=255)
                                                                                                                               acttaTCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacgacg                                                                                                                                                                                                                                                       >  2:627877/1‑188 (MQ=255)
                                                                                                                                    tCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGCATTGAAAGCGATAAATACACATTAAAATAGTTAGTCCGGGAATGAAACCGGGACCTGAACATGTTGTATTGCGTGAATg                                                                                                                                             >  2:1120505/1‑289 (MQ=255)
                                                                                                                                                  cagCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGa                                                                                                                               >  2:1035198/3‑289 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGCTTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                                                                                                                                                                                         >  2:412736/1‑212 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGt                                                                                                                                                                                                                                                                            >  1:1865488/1‑145 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGt                                                                                                                                                                                                                                                                            <  2:1865488/145‑1 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                                                                                                                                                                                         <  1:412736/212‑1 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                                                                                                                                                                           >  1:584435/1‑242 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                                                                                                                                                                           <  2:584435/242‑1 (MQ=255)
                                                                                                                                                     cacaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGAt                                                                                                                              >  1:1352046/1‑287 (MQ=255)
                                                                                                                                                      acaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTTAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATa                                                                                                                             <  2:771931/287‑1 (MQ=255)
                                                                                                                                                      acaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATa                                                                                                                             <  1:103792/287‑1 (MQ=255)
                                                                                                                                                      acaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGACAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATa                                                                                                                             <  1:1597896/287‑1 (MQ=255)
                                                                                                                                                      acaAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGATTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGACAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATa                                                                                                                             <  2:561264/287‑1 (MQ=255)
                                                                                                                                                       caAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTTAGTCCGGGAATGAAACCCGGACCTGAACATGTCCTATTTCGTGAATGTTTCTTTTATTTTATACTg                                                                                                                          >  2:260539/1‑289 (MQ=255)
                                                                                                                                                          gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATa                                                                                                                                                                                               <  1:1356986/217‑1 (MQ=255)
                                                                                                                                                          gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCAt                                                                                                                       >  2:555367/1‑289 (MQ=255)
                                                                                                                                                                    gTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGGATTGAAATAGATAAATACACATTAAAATAGTGAGTCCgg                                                                                                                                                                                     >  2:1356986/1‑217 (MQ=255)
                                                                                                                                                                          aTCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGa                                                                                                                                                <  2:1934134/248‑1 (MQ=255)
                                                                                                                                                                               ggTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGtttctt                                                                                                                                       <  1:91487/252‑1 (MQ=255)
                                                                                                                                                                               ggTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGtttctt                                                                                                                                       >  2:91487/1‑252 (MQ=255)
                                                                                                                                                                               ggTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCa                                                                                                                                    <  1:636506/255‑1 (MQ=255)
                                                                                                                                                                                          cTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACttt                                                                                         >  1:1016081/1‑287 (MQ=255)
                                                                                                                                                                                              acctcattttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCTGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGGTCAGTAAGCGAAATAAATTttctttt                                                                                   >  2:658629/7‑287 (MQ=255)
                                                                                                                                                                                                  cattttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCa                                                                                                          >  2:952846/3‑262 (MQ=255)
                                                                                                                                                                                                  cattttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCa                                                                                                          <  1:952846/260‑1 (MQ=255)
                                                                                                                                                                                                   attttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTttcttgttcttg                                                                              <  2:992069/288‑1 (MQ=255)
                                                                                                                                                                                                     tttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGct                                                                            <  2:1016081/289‑1 (MQ=255)
                                                                                                                                                                                                      ttttATGCGGGTTTGGCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGctt                                                                           <  1:547620/289‑1 (MQ=255)
                                                                                                                                                                                                         tATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCCATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGcttctt                                                                        <  2:1888020/289‑1 (MQ=255)
                                                                                                                                                                                                          aTGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCttcttc                                                                       <  2:1136268/289‑1 (MQ=255)
                                                                                                                                                                                                                gTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACttt                                                                  <  1:809525/288‑1 (MQ=255)
                                                                                                                                                                                                                 ttggCCAGTGCAAGTCCGGCGATCGACCGATAGTCAATTAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTGAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAGCATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTg                                                                 <  2:1114627/285‑1 (MQ=255)
                                                                                                                                                                                                                      cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTAccc                                                            <  1:529831/288‑1 (MQ=255)
                                                                                                                                                                                                                         ggtaaGCCCGGCACGCAATCGATAGTCAATGAGGATTATCTCAGCGAAAGCGGATGTGTTGGCTCGATGATGTATGTCGGGATTATTGAGTTACGACGCTGTCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATa                                                         <  2:1468568/285‑1 (MQ=255)
                                                                                                                                                                                                                            aaGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGaa                                                     <  1:784423/289‑1 (MQ=255)
                                                                                                                                                                                                                                  ggCACTCGACCGGTAGTCAATGAGTGTTATGTCAGCGAAAGCGACTTCTTAGGCTAGATGATGCAAGTCGGGATTATTGAGTTACGACTCTGACAGTCAGAGAATTGCCAGCAAGGATTGAAAGCGATAAATACACCATAAAATAGTGAGTCTGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCATTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTATTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTa                                               <  2:487997/289‑1 (MQ=255)
                                                                                                                                                                                                                                         gACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTc                                                                                                                                                                                        <  2:1327221/145‑1 (MQ=255)
                                                                                                                                                                                                                                         gACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTc                                                                                                                                                                                        >  1:1327221/1‑145 (MQ=255)
                                                                                                                                                                                                                                          aCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGcttct                                                                         >  1:772286/1‑255 (MQ=255)
                                                                                                                                                                                                                                                           aTTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGa                                                      >  2:833463/1‑257 (MQ=255)
                                                                                                                                                                                                                                                           aTTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTgccga                       <  1:543403/288‑4 (MQ=255)
                                                                                                                                                                                                                                                             tATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTgccgataa                    <  1:1120505/289‑7 (MQ=255)
                                                                                                                                                                                                                                                                  cAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCCGATAATTtta               <  1:1909416/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGtt                            >  2:1419816/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                      gAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGCGAAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCCGATAATTttatt             <  1:355971/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                     
CAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGAGTGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGAGTGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCAGTTTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCAGCATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGAGTGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTTATTGCGTGAATGTTTCTTTCATTTGATACTGCATTTCCCAGAGTTCAGTAAGGCTAATAACTTTTCTTGTTCTTGCTTCTTCACTTTGTACCCATAAGAAAGGGTAGAACGTGAAGGCTTCCGTTGCGCTTAATTTTATTACGTCTTCTCG  >  CP042583/587972‑588531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A