Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 655,723 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583655,7230GC99.6% 528.2 / NA 227intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (110/116);  total (111/116)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583655,7240CG99.6% 529.3 / NA 227intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (111/115);  total (111/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583655,7250TA100.0% 535.5 / NA 227intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (111/116);  total (111/116)

Only 100 of 227 total aligned reads displayed.

TGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCGCTAACGGCCTGATAAATGCCAATGCTATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTGCTTTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCACTAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCAATCCGCCAGCA  >  CP042583/655455‑656003
                                                                                                                                                                                                                                                                            |||                                                                                                                                                                                                                                                                                       
tGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGGCGATTTTCCTGCACCATTTg                                                                                                                                                                                                                                                                        >  1:982833/1‑288 (MQ=255)
   cGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAGAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGccc                                                                                                                                                                                                                                                                    <  1:1969678/289‑1 (MQ=255)
                ccACTGCTGCACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGACTCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTTCTGACCCGGTACTCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGCTGGTTTCGCCGCTGCCGCGACGTCGTCCGCAAAGCGTCAAATCGTTGCTGTAACCTTTCGGATGATCGCCATCAACCAGTCTTAATAACGTGGATGTTCATGCACCATTTGGCCCTAACATTTGCAAg                                                                                                                                                                                                                                                       >  2:1403410/1‑289 (MQ=255)
                ccACTGCTGCACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAg                                                                                                                                                                                                                                                       >  1:1653208/1‑289 (MQ=255)
                  aCTGCTGCACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtg                                                                                                                                                                                                                                                     <  2:1044254/289‑1 (MQ=255)
                  aCTGCTGCACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtg                                                                                                                                                                                                                                                     <  1:1333191/289‑1 (MQ=255)
                    tgctgcACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCCGGCTTAATACCGTCGATTTTCCTGCCCCATTTGGCCCGACACTTTGCCCgtgt                                                                                                                                                                                                                                                    >  2:313501/1‑288 (MQ=255)
                    tgctgcACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTgg                                                                                                                                                                                                                                                                       >  1:1933969/1‑269 (MQ=255)
                    tgctgcACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTTGCCCGACAATTTGCCAGtttt                                                                                                                                                                                                                                                   >  1:1783507/1‑289 (MQ=255)
                    tgctgcACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGtt                                                                                                                                                                                                                                                   <  1:684352/289‑1 (MQ=255)
                     gctgcACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGTTGGTTTCGCCGCTGCAGCGAAGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACATTGCGGAGGATAGCAAGTAACCAGGCTTAATAACGTCGATTTACCTGAACCATATGCCCAGACAATTTGCCAGTGtta                                                                                                                                                                                                                                                  >  2:1893013/1‑288 (MQ=255)
                     gctgcACCAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTc                                                                                                                                                                                                                                                  >  1:171514/1‑289 (MQ=255)
                            cAGTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGa                                                                                                                                                                                                                                           <  1:1445394/289‑1 (MQ=255)
                              gTTTTTGCTGGCGATCCGAAACGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGCtt                                                                                                                                                                                                                                         >  2:467389/1‑289 (MQ=255)
                                          gatccgaaaCGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCt                                                                                                                                                                                                                             <  2:38562/282‑1 (MQ=255)
                                           atccgaaaCGGCCAGGTAAAGGCAAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACAGTAGTGCTGACCCGGTCATCCAGATGCAAACTAATGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGAGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTa                                                                                                                                                                                                                            <  2:1071696/283‑1 (MQ=255)
                                           accccaaccGGCCATATAAAGGCAAATCGAATCAAAATAGCCAGAAAGAAACACATTACGCACGGTATTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTa                                                                                                                                                                                                                            <  2:45546/281‑1 (MQ=255)
                                            tccgaaaCGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTa                                                                                                                                                                                                                            <  1:1350784/283‑1 (MQ=255)
                                            tccgaaaCGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTa                                                                                                                                                                                                                            <  2:1465995/283‑1 (MQ=255)
                                              cgaaaCGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGACAGAATCACCTGTCGCACGGTAGTGCTGACCCGATAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCTTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAgg                                                                                                                                                                                                                         <  1:1456283/286‑1 (MQ=255)
                                              caaaaCGGCCTGATGAATGCACACTAAACCAAAAGAGCCAGAAAGCAACACATTACCCACGGTAGTGGTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAgg                                                                                                                                                                                                                         <  2:1420036/286‑1 (MQ=255)
                                                  aCGGCCTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGttat                                                                                                                                                                                                                     >  1:1112991/1‑289 (MQ=255)
                                                   cgtccTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGTTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGttatt                                                                                                                                                                                                                    <  1:1893013/286‑1 (MQ=255)
                                                      ccTGATAAATGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAg                                                                                                                                                                                                                 >  1:1981276/1‑289 (MQ=255)
                                                               tagcaatgaaaacAAAAGAGCCAGAACACAGCACATTACGCACGGTAGTGCTGACACGGTAATTCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGGCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGGGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCg                                                                                                                                                                                                        <  2:1917372/277‑1 (MQ=255)
                                                               tGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCg                                                                                                                                                                                                        <  1:329620/289‑1 (MQ=255)
                                                               tGCCAATCGAATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCg                                                                                                                                                                                                        >  2:1600137/1‑289 (MQ=255)
                                                                gccaatcgaaTCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGa                                                                                                                                                                                                       >  2:1665378/10‑289 (MQ=255)
                                                                    atcgaaTCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGc                                                                                                                                                                                                                                                                                >  2:1019225/6‑212 (MQ=255)
                                                                    atcgaaTCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGc                                                                                                                                                                                                                                                                                <  1:1019225/207‑1 (MQ=255)
                                                                             aaaTAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGaaa                                                                                                                                                                                          <  1:599388/289‑1 (MQ=255)
                                                                                tAGCCAGAAAGAACGCCATTACGGACGGTAGTGCTGACTCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTGTAGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCa                                                                                                                                                                                       <  2:1639821/289‑1 (MQ=255)
                                                                                        aagaaTCCCATTACGACCGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  2:874522/286‑1 (MQ=255)
                                                                                        aagaaTCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  1:290233/286‑1 (MQ=255)
                                                                                        aagaaTCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  1:1085887/286‑1 (MQ=255)
                                                                                        aagaaTCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  2:1981276/286‑1 (MQ=255)
                                                                                        aagaaTCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  1:1512892/286‑1 (MQ=255)
                                                                                          gcattgataaaCGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCCGCTAAGGTTATTAAGAATGGGGCGAACGTTATAAGAAACGACCCAATGGtt                                                                                                                                                                             >  2:797344/11‑289 (MQ=255)
                                                                                            aTCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAgg                                                                                                                                                                                                                         <  2:683611/243‑1 (MQ=255)
                                                                                                     cGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATg                                                                                                                                                                   >  1:1447793/1‑288 (MQ=255)
                                                                                                      gcccGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATgcg                                                                                                                                                                 <  1:466826/286‑1 (MQ=255)
                                                                                                        gcGGTAGGGCAGCCCCGGTAATCCATATGCACCCTATAGCGCACGTAACAGGTTGTCTTTTTGCGATCCCAGAGGGTGTCGCCGCGGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTACCCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGGTATAAGAAACCACGCCATTGTTCAGCACAATgcgc                                                                                                                                                                <  2:298063/287‑1 (MQ=255)
                                                                                                          ggTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTa                                                                                                                                                                                                                            <  2:1212726/226‑1 (MQ=255)
                                                                                                          ggTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCGGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGt                                                                                                                                                             >  1:469237/1‑289 (MQ=255)
                                                                                                               tGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAACTCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCCCCTGCCAGCTAAGGTTATTACGAAGGGGGCGAGCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTCCGtt                                                                                                                                                        >  2:1760077/1‑289 (MQ=255)
                                                                                                               tGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCa                                                                                                                                                                                       <  2:559841/258‑1 (MQ=255)
                                                                                                               tGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGtt                                                                                                                                                        >  2:1536701/1‑289 (MQ=255)
                                                                                                               tGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGtt                                                                                                                                                        >  2:186561/1‑289 (MQ=255)
                                                                                                                    aCCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTtata                                                                                                                                                                                               >  1:1867716/1‑245 (MQ=255)
                                                                                                                    aCCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgg                                                                                                                                                   >  2:1779981/1‑289 (MQ=255)
                                                                                                                    aCCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCCCCTGGATTCCCCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTTTTCAGCACAAAGTGCGGTTCGTTGGCgg                                                                                                                                                   >  2:588609/1‑289 (MQ=255)
                                                                                                                      ccGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGt                                                                                                                                                 >  2:334537/1‑289 (MQ=255)
                                                                                                                               cAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGa                                                                                                                                        <  2:1818534/289‑1 (MQ=255)
                                                                                                                                  aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGccc                                                                                                                                                                                                                                                                    >  2:1251476/1‑162 (MQ=255)
                                                                                                                                  aTGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGccc                                                                                                                                                                                                                                                                    <  1:1251476/162‑1 (MQ=255)
                                                                                                                                   tGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACg                                                                                                                                      <  1:1172694/287‑1 (MQ=255)
                                                                                                                                        actactGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGtt                                                                                                                                                                             >  2:569006/1‑243 (MQ=255)
                                                                                                                                        actactGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGtt                                                                                                                                                                             <  1:569006/243‑1 (MQ=255)
                                                                                                                                                tgACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCAt                                                                                                                        <  2:1447793/288‑1 (MQ=255)
                                                                                                                                                         ggttattgtttttGATATCCCAGATGGTTTCGCCTCTGCCGCGACGTCGTCCGAAAAGCGTAAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCAtccggctccgg                                                                                                              <  2:350009/281‑1 (MQ=255)
                                                                                                                                                            tatGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCTGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAAAAACCCCGCCATTGTGCAGCACAATGCGCGGTTGGTTGGCGGGTTATGCGTAACGTGCTGGAGGTTCATCCGGCTCCGGCAg                                                                                                           >  2:250199/1‑289 (MQ=255)
                                                                                                                                                              tGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  2:279578/216‑1 (MQ=255)
                                                                                                                                                              tGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGtt                                                                                                         >  2:128371/1‑289 (MQ=255)
                                                                                                                                                                 tttttGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGca                                                                                                      <  1:1600137/289‑1 (MQ=255)
                                                                                                                                                                 tttttGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGca                                                                                                      <  1:1665378/289‑1 (MQ=255)
                                                                                                                                                                 tttttGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGca                                                                                                      <  1:1189000/289‑1 (MQ=255)
                                                                                                                                                                 tttttGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGca                                                                                                      <  1:223135/289‑1 (MQ=255)
                                                                                                                                                                 tttttGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGca                                                                                                      <  1:811141/289‑1 (MQ=255)
                                                                                                                                                                 tttttGATATCCCAGATGGTCTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTGTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGca                                                                                                      <  1:719689/289‑1 (MQ=255)
                                                                                                                                                                        tatCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTc                                                                                               <  1:1226658/289‑1 (MQ=255)
                                                                                                                                                                          tCCCATGTGGTTTCGCCGCTGCCGCGACGTCGTCCGAAACGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCaa                                                                                             <  2:1677059/289‑1 (MQ=255)
                                                                                                                                                                           cccAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAg                                                                                            <  1:137483/289‑1 (MQ=255)
                                                                                                                                                                                 tGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGtt                                                                                       <  1:1503570/288‑1 (MQ=255)
                                                                                                                                                                                 tGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCG‑CAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCa                                                                                     <  1:35648/289‑1 (MQ=255)
                                                                                                                                                                                    tttCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCgggg                                                                                                                                                 >  2:1152373/1‑226 (MQ=255)
                                                                                                                                                                                      tCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCCCGCCATTGTTCAGCCCAATGCGCGGTCCGTTGGCGGGTCAGGCCTGACGTGCTGAAGGTCCCTCCGGCTCCCGCAGCTGCCCACCTTCAACCTGCTCaaca                                                                                  >  2:1743463/1‑285 (MQ=255)
                                                                                                                                                                                      tCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGt                                                                                        >  1:671497/1‑282 (MQ=255)
                                                                                                                                                                                      tCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGt                                                                                        <  2:671497/282‑1 (MQ=255)
                                                                                                                                                                                                       tcgtcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGtt                                                                                                                                                                                                                                                   >  2:1541038/1‑110 (MQ=255)
                                                                                                                                                                                                           ccGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTg                                                            <  1:629587/289‑1 (MQ=255)
                                                                                                                                                                                                               aaaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGGGCttg                                                        >  2:536201/1‑289 (MQ=255)
                                                                                                                                                                                                                     tCAAATCGTTGCTGTAACCTTGCGGATGGTCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCCCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGAAGTTGCACACCTTCAAGCTTTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGa                                                   >  1:1931880/1‑288 (MQ=255)
                                                                                                                                                                                                                     tCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGAGCGACGAGTGCTTGTTGGa                                                   >  1:1320326/1‑288 (MQ=255)
                                                                                                                                                                                                                      cAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAg                                                  <  1:268715/288‑1 (MQ=255)
                                                                                                                                                                                                                            gTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTcc                                           <  1:797344/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aCCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGATGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGGTCCCCTTTAgc                                   >  1:1526559/1‑288 (MQ=255)
                                                                                                                                                                                                                                          gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGtt                                                                                       <  1:852887/231‑1 (MQ=255)
                                                                                                                                                                                                                                          gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTACCGCCa                               >  2:1103566/1‑287 (MQ=255)
                                                                                                                                                                                                                                          gCGGATGATCGCCAGTAACAAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGAGCGACGAGTGCTTGTTGGAGCAGTTCATCTTTATCTCCA‑Gt                            >  1:1066529/1‑289 (MQ=255)
                                                                                                                                                                                                                                           cGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCt                                                          >  1:320023/1‑259 (MQ=255)
                                                                                                                                                                                                                                           cGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑Gtt                           >  1:1480827/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   cGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCCGCAGTTGCGCGACGAGTGCTTGCTTGAGCAGCTGCTCTTTAGCGCCATGTTCCCGca                     >  2:693872/1‑287 (MQ=255)
                                                                                                                                                                                                                                                        gTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGATTCATCCGGCTCCGGCAGTTGCACACCTTCCAGCTGGTCACTATGCGCCAGTTGCGCCACGAGTGCTTGGTGGAGCCGTTACCCTTTTGCGCCA‑GTTTCCGCTCACGTGc              >  2:419351/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          acacAGGCTTAATGATGTCGATGTTCCTGCACCATTTGGCCCGACAATTGGCCAGTGTTGGCCGGGATGTACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCaa            <  2:1330087/286‑1 (MQ=255)
                                                                                                                                                                                                                                                           aCCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCa                                                                                                                                                                                  <  1:1836965/123‑1 (MQ=255)
                                                                                                                                                                                                                                                                gCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCAATCcgc       >  2:638400/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                  ttAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGATTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCAATCcgccag    <  1:419351/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   taataaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACTAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCAATCcgccagc   >  2:849320/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    aataaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTTACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCAATCcgccagc   >  2:1141758/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    aataaCGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCCTGTTGGAGCAGTTCCTCTTTCGCGCCA‑GTTTCCGCTAACGTGCCGTCcgccgtg   >  2:1562493/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                            |||                                                                                                                                                                                                                                                                                       
TGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTTGCTGGCGATCGCTAACGGCCTGATAAATGCCAATGCTATCAAAATAGCCAGAAAGAATCACATTACGCACGGTAGTGCTGACCCGGTAATCCAGATGCAAACTACTGCTGACGTAACCGATATGCTTTTTGATATCCCAGATGGTTTCGCCGCTGCCGCGACGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTGCTTTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTCACCTGCCAGCTAAGGTTATTAAGAATGGGGCGATCGTTATAAGAAACCACGCCATTGTTCAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCACTAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCA‑GTTTCCGCTAACGTGCAATCCGCCAGCA  >  CP042583/655455‑656003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A